A novel polysaccharide AAMP-A70 (5.6 kDa) has been purified from the fruiting bodies of Amillariella mellea. Compositional analysis and 1H and 13C NMR spectra indicate that AAMP-A70 is a branched β-glucan with a main chain that consists of β-D-(1→6) linked Glcp residues substituted at O-3 by β-Glcp or α-D-(1→6)-linked Galp side chains. AAMP-A70 increases macrophage phagocytosis and secretion of NO, ROS, TNF-α, IL-6 and IL-1β. Mechanistically, AAMP-A70 promotes degradation of I kappa B-alpha and nuclear translocation of the NF-kappa B p65 subunit, and enhances phosphorylation of MAPKs. In particular, the function blocking antibody to TLR2 substantially suppresses TNF-α and IL-6 production. Our data demonstrate that AAMP-A70 activates macrophages via NF-kappa B/MAPK signaling pathways and the TLR2 receptor. Overall, AAMP-A70 may serve as a good food supplement to enhance immunity.

polysaccharides, oxidative stress, Morchella conica

• Compound ID: 21858
  

  b-Glcp-(1-6)-?%a-D-Galp-(1-3)-+ | -6)-b-D-Glcp-(1-6)-b-D-Glcp-(1-6)-b-D-Glcp-(1-6)-b-D-Glcp-(1-

  Show graphically

  Show legend Show as text

  Structure type: structural motif or average structure ; 5600
  Reference(s) to other database(s): GTC:G79943VS
  
   
  
  Amillariella mellea
  CSDB ID 49147 (all data & tools)