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1. (Article ID: 8843)
Choukade R, Kango N
Characterization of a mycelial fructosyltransferase from Aspergillus tamarii NKRC 1229 for efficient synthesis of fructooligosaccharides
Food Chemistry 286 (2019)
434-440
An efficient system for biotransformation of sucrose to fructooligosaccharides (FOS) was obtained using Aspergillus tamarii NKRC 1229 mycelial fructosyltransferase (m-FTase). Zymographic analysis confirmed mycelial localization of the FTase (36 U/g) and lyophilized fungal pellets were used for bioconversion. m-FTase had molecular weight ~75 kDa with optimum activity at pH 7.0 and 20 °C. FOS production after parametric optimization (sucrose - 50% w/v, m-FTase dose - 4.5% w/v, inoculum age - 48 h and incubation time - 24 h) reached 325 g/L (55% yield) with 14% residual sucrose, 25% glucose and 6% fructose. FTase activity was enhanced after pre-treatment with organic solvents and SDS. FOS was purified in a single step using gel filtration matrix, Bio-Gel P2. FOS was characterized using Diffusion ordered spectroscopy-Nuclear Magnetic Resonance (1H DOSY-NMR) and Fourier-transform infrared spectroscopy (FTIR). Continuous generation of FOS was achieved using recyclable mycelia upto 10 consecutive cycles.
Prebiotics, Aspergillus, fructooligosaccharides (FOS), fructosyltransferase (FTase), zymography, Bio-Gel P2
NCBI PubMed ID: 30827630Publication DOI: 10.1016/j.foodchem.2019.02.025Journal NLM ID: 7702639Publisher: Elsevier Applied Science Publishers
Correspondence: Choukade R
gmail.com>; Kango N gmail.com>
Institutions: Enzyme Technology and Molecular Catalysis Laboratory, Department of Microbiology, Dr. Harisingh Gour Vishwavidyalaya (A Central University), Sagar, India
Methods: 13C NMR, IR, SDS-PAGE, DNA techniques, TLC, HPLC, enzymatic digestion, colorimetry, extraction, CC, zymographic analysis, dialysis, HPLC-SEC, enzymatic assay, DOSY, precipitation, sonication, centrifugation, dinitrosalicylic acid (DNS) method, BLAST
The publication contains the following compound(s):
- Compound ID: 347
Structure type: oligomer
Trivial name: 1-kestose, inulin
Compound class: fructan, fructo-oligosaccharide
Reference(s) to other database(s): GTC:G11260CN, GlycomeDB:
396, CCSD:
26122, CBank-STR:5624, GenDB:MH445969, US-PT:US2017298332A1
- Compound ID: 547
Structure type: oligomer
Trivial name: nystose, inulin, 1,1-kestotetraose
Compound class: fructan
Reference(s) to other database(s): GTC:G60726JL, GlycomeDB:
63, CCSD:
23519, CBank-STR:9595, GenDB:MH445969, US-PT:US2017298332A1
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