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1. (Article ID: 8858)
 
Tódero LM, Rechia CGV, Guimarães LHS
Production of short-chain fructooligosaccharides (scFOS) using extracellular β-D-fructofuranosidase produced by Aspergillus thermomutatus
Journal of Food Biochemistry 43(8) (2019) ID e12937
 

Aspergillus thermomutatus produces an extracellular β-D-fructofuranosidase when cultured in Khanna medium with sucrose as additional carbon source at 30°C under agitation for 72 hr. Addition of glucose and fructose in the culture medium affected the production of the enzyme negatively. The optimum hydrolytic activity was achieved at 60°C and pH 5.0, with half-life (T50) of 30 hr at 50°C and 62% of its activity maintained at pH 5.0 for 48 hr. The extracellular extract containing β-D-fructofuranosidase was effective in producing fructooligosaccharides (FOS), mainly 1-kestose. The highest concentration of FOS was obtained at 30°C and 60°C, indicating the existence of at least two enzymes with transfructosylating activity. At 30°C, the maximal FOS concentration was obtained from 48 to 72 hr, while at 60°C, it was achieved only at 72 hr. The best production of FOS (86.7 g/L) was obtained using 500 g/L sucrose as substrate.

Aspergillus, fructooligosaccharide, transfructosylating, β-D-fructofuranosidase

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