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1. (Article ID: 8862)
 
Zhang S, Jiang H, Xue S, Ge N, Sun Y, Liu G, Chi Z
Efficient conversion of cane molasses into fructooligosaccharides by a glucose derepression mutant of Aureobasidium melanogenum with high β-fructofuranosidase activity
Journal of Agricultural and Food Chemistry 67(49) (2019) 13665-13672
 

Fructooligosaccharides (FOSs) are excellent food ingredients or feed additives by stimulating probiotics. In this paper, a CREA gene encoding a glucose repressor in the β-fructofuranosidase producer Aureobasidium melanogenum 33 with high-level FOS biosynthesis was disrupted, and glucose repression in disruptant D28 was relieved. The disruptant D28 produced up to 2100 U/mL of β-fructofuranosidase activity, whereas the enzyme activities produced by parent strain 33 and complemented strain C11 were below 600 U/mL. The whole cells of the disruptant D28 was used to convert cane molasses into FOSs, and 0.58 g of FOSs/g of molasses sugar was synthesized from 350 g/L cane molasses sugar within 4 h. Results demonstrated that the industrial waste cane molasses can be efficiently converted into FOSs by the glucose derepression mutant D28 with high β-fructofuranosidase activity. This low-cost and environmentally friendly bioprocess has great potential applications in bioengineering and biotechnology for FOS production.

Aureobasidium melanogenum, cane molasses, glucose derepression, high-level FOS biosynthesis

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