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Structure type: polymer chemical repeating unit
Trivial name: sphingan, S-198
Compound class: EPS
Contained glycoepitopes: IEDB_115136,IEDB_136105,IEDB_140630,IEDB_142488,IEDB_146664,IEDB_189517,IEDB_225177,IEDB_423153,IEDB_885823,IEDB_983931,SB_192

• Article ID: 253
  Hashimoto W, Murata K "a-L-rhamnosidase of Sphingomonas sp. R1 producing an unusual exopolysaccharide of sphingan" - Bioscience, Biotechnology, and Biochemistry 62(6) (1998) 1068-1074
  

  A soil bacterium with α-L-rhamnosidase was isolated from a cumulative mixed culture containing a polysaccharide of gellan as a carbon source and identified to be Sphingomonas paucimobilis, known as a potent producer of gellan. The isolate (designated Sphingomonas sp. R1) produced an unusual exopolysaccharide of sphingan (denoted HWR1) distinct from gellan. The rhamnose in gellan was replaced with mannose in HWR1. The bacterium had a peculiar cell surface covered with many complicated plaits. α-L-Rhamnosidase purified from Sphingomonas sp. R1 grown in the presence of naringin was a monomer with a molecular mass of 110 kDa and most active at pH 8.0 and 50 degrees C. The enzyme required divalent metal ions for the activity and released L-rhamnose from various rhamnosyl glycosides.

  polysaccharide, Sphingomonas, exopolysaccharide, sphingan, a-L-rhamnosidase

  NCBI PubMed ID: 9692187
  Publication DOI: 10.1271/bbb.62.1068
  Journal NLM ID: 9205717
  Publisher: Japan Society for Bioscience, Biotechnology, and Agrochemistry
  Correspondence: hasimoto@food2.food.kyoto-u.ac.jp
  Institutions: Research Institute for Food Science, Kyoto University, Uji 611-0011, Japan
  Methods: SDS-PAGE, TLC, acid hydrolysis, HPLC, electron microscopy, enzyme assay
  
   
  
  Sphingomonas
  CSDB ID 31512 (all data & tools)
• Article ID: 2076
  Chowdhury TA, Lindberg B, Lindquist U, Baird J "Structural studies of an extracellular polysaccharide (S-198) elaborated by alcaligenes ATCC 31853" - Carbohydrate Research 161 (1987) 127-132
  
  Journal NLM ID: 0043535
  Publisher: Elsevier
  
   
  
  Alcaligenes sp. ATCC 31853
  CSDB ID 115032 (all data & tools)

Show legend Show as text

Structure type: polymer chemical repeating unit
Compound class: EPS
Contained glycoepitopes: IEDB_115136,IEDB_136105,IEDB_1394182,IEDB_140630,IEDB_142488,IEDB_146664,IEDB_225177,IEDB_423153,IEDB_885823,IEDB_983930,IEDB_983931,SB_192

• Article ID: 2076
  Chowdhury TA, Lindberg B, Lindquist U, Baird J "Structural studies of an extracellular polysaccharide (S-198) elaborated by alcaligenes ATCC 31853" - Carbohydrate Research 161 (1987) 127-132
  
  Journal NLM ID: 0043535
  Publisher: Elsevier
  
   
  
  Alcaligenes sp. ATCC 31853
  CSDB ID 115031 (all data & tools)