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1. (Article ID: 9000)
Terán Hilares R, Resende J, Orsi CA, Ahmed MA, Lacerda TM, da Silva SS, Santos JC
Exopolysaccharide (pullulan) production from sugarcane bagasse hydrolysate aiming to favor the development of biorefineries
International Journal of Biological Macromolecules 127 (2019)
169-177
Pullulan is a biopolymer used in food industry produced by Aureobasidium pullulans from starch. In the present work, sugarcane bagasse (SCB) hydrolysate was evaluated as an alternative substrate in fermentation process assisted by blue LED lights. The best fermentation conditions in Erlenmeyer flasks were 25.3 °C, stirring speed of 232 rpm and yeast extract concentration of 1.88 g/L, yielding 25.19 g/L of pullulan, that corresponded to yield of 0.48 g/g and 0.28 g/(L·h) of volumetric productivity. By using a column bubble photobioreactor, similar yield values were obtained. Thermal properties of the produced pullulan as glass transition (Tg) and melting (Tm) temperatures were 38 °C and 160 °C, respectively, which were similar to the corresponding values of commercial food grade pullulan. Therefore, SCB hydrolysate is a promising substrate to produce good quality pullulan (86% of purity) with application in food industry, besides to represent a new alternative for biorefineries.
pullulan, biorefinery, sugarcane bagasse
NCBI PubMed ID: 30639656Publication DOI: 10.1016/j.ijbiomac.2019.01.038Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: teran-2017
usp.br
Institutions: Department of Biotechnology, Engineering School of Lorena, University of São Paulo, Lorena, Brazil, Department of chemical engineering, MNS University of Engineering and Technology, Multan, Pakistan
Methods: HPLC, GPC, enzymatic digestion, differential scanning calorimetry (DSC), viscosity measurement, cell growth, precipitation, spectrophotometry, centrifugation, optical density measurement, thermogravimetric analysis
The publication contains the following compound(s):
- Compound ID: 12430
Structure type: polymer chemical repeating unit
Trivial name: pullulan
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide
Reference(s) to other database(s): GTC:G71532WE, CCSD:
45938, CBank-STR:4859, GenDB:KY767023; GenDB:KY767024
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2. (Article ID: 9047)
Singh RS, Kaur N, Kennedy JF
Pullulan production from agro-industrial waste and its applications in food industry: A review
Carbohydrate Polymers 217 (2019)
46-57
Pullulan is a microbial exopolysaccharide produced from Aureobasidium pullulans by submerged fermentation of a medium supplemented with carbon, nitrogen and other essential nutrients. These nutrients are expensive which increase the cost of pullulan production. The requirement of alternative cost-effective substrates for pullulan production is a prerequisite. Agro-based industries generate a large volume of solid/liquid waste and its accumulation generates a severe environmental impact. These wastes are composed of carbohydrates, proteins and other constituents, and can be used as substrates for the development of low-cost processes for the production of various microbial products. This could be a good environmental friendly waste management system. Pullulan production from agro-industrial wastes can be carried out by both submerged and solid-state fermentation by A. pullulans. Owing to its unique properties, pullulan has wide applications in many food-based industries. This review highlights pullulan production from agro-industrial wastes and potential applications of pullulan in various food industries.
pullulan, Aureobasidium pullulans, submerged fermentation, solid-state fermentation, agro-industrial waste
NCBI PubMed ID: 31079684Publication DOI: 10.1016/j.carbpol.2019.04.050Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Singh RS
pbi.ac.in>, Singh RS lycos.com>
Institutions: Carbohydrate and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala, India, Chembiotech Laboratories, Advanced Science and Technology Institute, Bromsgrove, UK
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