Pullulan is a microbial exopolysaccharide produced from Aureobasidium pullulans by submerged fermentation of a medium supplemented with carbon, nitrogen and other essential nutrients. These nutrients are expensive which increase the cost of pullulan production. The requirement of alternative cost-effective substrates for pullulan production is a prerequisite. Agro-based industries generate a large volume of solid/liquid waste and its accumulation generates a severe environmental impact. These wastes are composed of carbohydrates, proteins and other constituents, and can be used as substrates for the development of low-cost processes for the production of various microbial products. This could be a good environmental friendly waste management system. Pullulan production from agro-industrial wastes can be carried out by both submerged and solid-state fermentation by A. pullulans. Owing to its unique properties, pullulan has wide applications in many food-based industries. This review highlights pullulan production from agro-industrial wastes and potential applications of pullulan in various food industries.

pullulan, Aureobasidium pullulans, submerged fermentation, solid-state fermentation, agro-industrial waste

• Compound ID: 22175
  

  -6)-a-D-Glcp-(1-4)-a-D-Glcp-(1-4)-a-D-Glcp-(1-

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  Structure type: polymer chemical repeating unit ; 45000-600000
  Trivial name: pullulan
  Compound class: glucan, polysaccharide
  Reference(s) to other database(s): GTC:G71532WE
  
   
  
  Aureobasidium pullulans P 56, Aureobasidium pullulans MTCC 2195, Aureobasidium pullulans MTCC 1991, Aureobasidium pullulans MTCC 2670, Aureobasidium pullulans RBF 4A3, Aureobasidium pullulans ATCC 42023, Aureobasidium pullulans MTCC 6994, Aureobasidium pullulans NRRLY 6220, Aureobasidium pullulans NCIM 1049, Aureobasidium pullulans 201253, Aureobasidium pullulans CCTCCM2012259, Aureobasidium pullulans HP-2001, Aureobasidium pullulans LB83, Aureobasidium pullulans AP329
  CSDB ID 50082 (all data & tools)

In the present investigation, polyethylene glycol (PEG 6000) was used for downstream processing of pullulan from Aureobasidium pullulans by aqueous phase separation (APS) technique. The cell-free broth was processed with PEG solution (10-35%, w/w) and pullulan formed a clear separate phase with all concentrations of PEG i.e. pullulan in lower phase and PEG solution along with impurities in upper phase. Maximum pullulan recovery from cell-free broth was obtained by PEG 25% (w/w). The sample handling in APS technique is quite easier due to lesser volume of PEG used in comparison to organic solvent precipitation i.e. minimum required ratio of cell-free broth to PEG for maximum pullulan yield was 0.5:1 (PEG:cell-free broth). Additionally, APS technique was found to be temperature independent. Further, structural attributes of pullulan recovered by APS was confirmed by FTIR, NMR and TLC. This is the first report on downstream processing of pullulan using PEG based aqueous solution.

pullulan, Aureobasidium pullulans, aqueous phase separation, polyethylene glycol

• Compound ID: 12430
  

  -6)-a-D-Glcp-(1-4)-a-D-Glcp-(1-4)-a-D-Glcp-(1-

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  Structure type: polymer chemical repeating unit
  Trivial name: pullulan
  Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide
  Reference(s) to other database(s): GTC:G71532WE, CCSD:45938, CBank-STR:4859, GenDB:KY767023; GenDB:KY767024
  
   
  
  Aureobasidium pullulans MTCC 6994
  CSDB ID 50083 (all data & tools)