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1. (Article ID: 913)
 
Lam JS, Rocchetta HL, Burrows LL
Glycosyltransferases of Pseudomonas aeruginosa that assemble the O antigens of A band and B band lipopolysaccharide
Journal of Endotoxin Research (1999) 96-101
 

Pseudomonas aeruginosa produces two forms of lipopolysaccharide (LPS) designated A band and B band. The O-polysaccharide region of A band is a conserved D-rhamnan polymer arranged alfa1-2,alfa1-3,alfa1-3, while B band is serotype-specific with differences in the O-antigenic region dividing P. aeruginosa into 20 stereotypes. The B band O-antigen unit of serotype O5 is [-4)bDMan(2NAc3N)A(1-4)bDMan(2NAc3NAc)A(1-3)aDFuc2NAc]. The glycosidic structure of LPS molecules specified by the action of dedicated glycosyltransferases. The wbp clusters of A band and B band (serotype O5) were each found to contain three genes coding for putative glycosyltransferases: wbpX, wbpY, wbpZ, and wbpH, wbpJ, wbpL, respectively. To examine the role of these potential transferases in LPS assembly, chromosomal mutaions were generated within all 6 genes. LPS analysis reveals that wbpX, wbpY and wbpZ mutants express and A-B+ phenotype, while wbpH and wbpJ metanst are A+B-. Interstingly, mutations in wbpL, and Escherichia coli wecA homologue, abrogates both A band and B band LPS synthesis, Based on amino acid homologies, O-polysaccharide structures and LPS phenotypes of transferase mutants, we propose and assembly scheme for these two LPS molecules.

Lipopolysaccharide, biosynthesis, genetic, antigen, characterization, O-antigen, B-band, O antigen, Pseudomonas, Pseudomonas aeruginosa, antigens, O antigens, O-antigens, glycosyltransferases, biochemical, review, glycosyltransferase, operon, A-band

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