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1. (Article ID: 9136)
 
Singh RS, Singh T, Pandey A
Fungal endoinulinase production from raw Asparagus inulin for the production of fructooligosaccharides
Bioresource Technology Reports 10 (2020) ID 100417
 

In present study, statistical optimization of medium composition for endoinulinase production from raw inulin using Aspergillus tritici was performed in shake-flask fermentations. The concentration of four independent variables viz. raw inulin (1.5–3.5%), peptone (0.2–1.8%), (NH4)H2PO4 (0.2–1.8%) and pH (4.5–6.5) was explored to optimize the composition of fermentation medium for maximum endoinulinase production. Fermentation medium containing 2.5% raw inulin, 1% peptone, 1% (NH4)H2PO4 and medium pH adjusted to 5.5 supported maximum (25.39 IU/mL) endoinulinase production. Adequate precision value >4, closeness of coefficient of determination (R2) value to 1, good co-relation between the predicted and experimental values, percentage error <5%, high F-value 164.52 and low Lack of fit F-value 0.19 authenticates the fitness of quadratic model. TLC fingerprints studies confirmed fructooligosaccharides synthesis from inulin by crude fungal endoinulinase. Fructooligosaccharides yield (19.40%) containing 3.70% ketose (GF2), 2.71% nystose (GF3) and 1.42% fructofuranosyl nystose (GF4) were obtained from inulin (10%).

response surface methodology, fructooligosaccharides, endoinulinase, Aspergillus tritici, raw-inulin

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2. (Article ID: 9137)
 
Singh RS, Singh T, Kennedy JF
Purification, thermodynamics and kinetic characterization of fungal endoinulinase for the production of fructooligosaccharides from inulin
International Journal of Biological Macromolecules 164 (2020) 3535-3545
 

Three-step purification technique (isopropanol precipitation, ion-exchange and size-exclusion chromatography) was used for the purification of an endoinulinase from the culture broth of Aspergillus tritici BGPUP6. The molecular mass of purified endoinulinase was found to be 53.45 kDa and 53.70 kDa by denatured protein gel (SDS-PAGE) and size-exclusion (Sephadex G-100) chromatographic analysis, respectively. Higher Km (0.90 mM), Vmax (19.60 mM/min·mg), Kcat (0.0013 1/min) and Vmax/Km ratio (21.77 1/min·mg) of purified endoinulinase for inulin than stachyose depicts its higher affinity towards inulin. Purified enzyme was found stable in a pH range 4.0-7.0 with an optimal pH 5.5. The optimal temperature of purified biocatalyst was 55 °C with thermostability in the range of 50-70 °C. D-value and Z-value for endoinulinase at 55 °C was found to be 100.08 h and 11.62 °C, respectively. Thermodynamics inactivation parameters (ΔG, ΔH and ΔS) of endoinulinase shows its wide range thermal stability. Endoinulinase activity was enhanced by CaCl2 and MnSO4, while CuSO4, CoCl2, AgNO3, CdCl2, NiCl2, ZnSO4, BaCl2, HgCl2 and EDTA inhibited the activity of enzyme. Purified endoinulinase was successfully used for the production of fructooligosaccharides from inulin.

fructooligosaccharides, endoinulinase, Aspergillus tritici, kinetics characterization

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