Found 1 publication.
Displayed publication 1
Expand all publications
Collapse all publications
Show all as text (SweetDB notation)
Show all graphically (SNFG notation)
1. (Article ID: 9171)
Li H, Zhao Y, Yang W, Zhang Z
Characterization of astragaloside I-IV based on the separation of HPTLC from Pleurotus ostreatus cultivated with Astragalus
Journal of Food Science 85(10) (2020)
3183-3190
In this study, total saponins were extracted from Pleurotus ostreatus cultivated with Astragalus as one of organic culture substrates. High Performance Thin Layer Chromatography (HPTLC) assay showed total saponins could be separated effectively, and four kinds of spots were identified as AG I, AG II, AG III, and AG IV, respectively. FTIR spectra based on HPTLC separation assay showed the saponin characteristic groups including -OH, C-H, C=O, and the glycoside linkaged to sapogenin group C-O-C, suggesting the four kinds of spots belonged to cycloartane-type triterpene saponins. The primary mass spectra of precursor ion (HPTLC-ESI-MS) assay further proved the main composition of four kinds of spots was AG I-IV, respectively. Physical properties, including the detection of specific rotation and melting point, revealed the separation of high-purity saponin monomer by HPTLC. HPTLC-dual wavelength spectrodensitometric method detection showed that content of astragaloside I-IV was ranged from 0.2 to 0.5 mg/g, and the total astragalosides contents attained to 1.397 mg/g, indicating P. ostreatus could bioaccumulate astragalosides from Astragalus. These results demonstrated the characterization of astragalosides based on the separation of HPTLC was effective, and supported to consider astragalosides-enriched P. ostreatus as functional edible fungus for food and medical applications. Currently, the consumption of enriched foods has become common and continues to increase due to urgent demanding for foods with high nutritional value. Pleurotus ostreatus is a functional edible fungus, which not only can produce secondary metabolites, but can enrich bioactive ingredients. Astragalosides have a wide range of biological activities, especially currently being tested as cardioprotective agent. In this study, P. ostreatus was cultivated through adding Astragalus into culture substrates, which realized massive enrichment of astragalosides. Astragalosides-enriched P. ostreatus as functional edible fungus could be extensively used in food and medical areas, especially for the prevention of cardiovascular diseases.
Pleurotus ostreatus, Astragalus, HPTLC, HPTLC-ESI-MS
NCBI PubMed ID: 32856317Publication DOI: 10.1111/1750-3841.15398Journal NLM ID: 0014052Publisher: Institute of Food Technologists
Correspondence: Zhang Z
163.com>
Institutions: School of Chemical Engineering and Technology, North University of China, Taiyuan, China, Provincial and Local Joint Cultivation Base, Shanxi Key Laboratory of Phytochemistry in Lvliang, Shanxi Engineering Research Center of Plant Functional Composition, Department of Life Science, Lvliang College, Lishi, China
Methods: IR, extraction, optical rotation measurement, HPTLC, melting point determination, evaporation, sonication, centrifugation, optical density measurement, filtration, HPTLC-ESI-MS
The publication contains the following compound(s):
- Compound ID: 22357
|
b-D-Glcp-(1-6)-+
|
b-D-Xylp2Ac3Ac-(1-3)-Subst
Subst = 20R,24S-epoxy-9,19-cyclolanostane-3β,6α,16β,25-tetrol, astramembrangenin, cycloastragenol SMILES CC1(C){3}[C@@H](O)CC[C@]2(C3)C43CC[C@]5(C)[C@@H]([C@](C)(O6)CC[C@H]6{25}C(C)(O)C){16}[C@@H](O)C[C@](C)5[C@]4([H])C{6}[C@H](O)[C@@]12[H] |
Show graphically |
Structure type: oligomer
; 891.7 [M+Na]+
Trivial name: astragaloside I
Compound class: glycoside
- Compound ID: 22359
|
b-D-Glcp-(1-6)-+
|
b-D-Xylp2Ac-(1-3)-Subst
Subst = 20R,24S-epoxy-9,19-cyclolanostane-3β,6α,16β,25-tetrol, astramembrangenin, cycloastragenol SMILES CC1(C){3}[C@@H](O)CC[C@]2(C3)C43CC[C@]5(C)[C@@H]([C@](C)(O6)CC[C@H]6{25}C(C)(O)C){16}[C@@H](O)C[C@](C)5[C@]4([H])C{6}[C@H](O)[C@@]12[H] |
Show graphically |
Structure type: oligomer
; 825.8 [M+H]+, 849.7 [M+Na]+
Trivial name: astragaloside II
Compound class: glycoside
- Compound ID: 22361
|
b-D-Glcp-(1-2)-b-D-Xylp-(1-3)-Subst
Subst = 20R,24S-epoxy-9,19-cyclolanostane-3β,6α,16β,25-tetrol, astramembrangenin, cycloastragenol SMILES CC1(C){3}[C@@H](O)CC[C@]2(C3)C43CC[C@]5(C)[C@@H]([C@](C)(O6)CC[C@H]6{25}C(C)(O)C){16}[C@@H](O)C[C@](C)5[C@]4([H])C{6}[C@H](O)[C@@]12[H] |
Show graphically |
Structure type: oligomer
; 807.6 [M+Na]+
Trivial name: astragaloside III
Compound class: glycoside
- Compound ID: 22362
|
b-D-Glcp-(1-6)-+
|
b-D-Xylp-(1-3)-Subst
Subst = 20R,24S-epoxy-9,19-cyclolanostane-3β,6α,16β,25-tetrol, astramembrangenin, cycloastragenol SMILES CC1(C){3}[C@@H](O)CC[C@]2(C3)C43CC[C@]5(C)[C@@H]([C@](C)(O6)CC[C@H]6{25}C(C)(O)C){16}[C@@H](O)C[C@](C)5[C@]4([H])C{6}[C@H](O)[C@@]12[H] |
Show graphically |
Structure type: oligomer
; 807.6 [M+Na]+
Trivial name: astragaloside IV
Compound class: glycoside
- Compound ID: 22358
|
b-D-Glcp-(1-6)-+
|
b-D-Xylp2Ac4Ac-(1-3)-Subst
Subst = 20R,24S-epoxy-9,19-cyclolanostane-3β,6α,16β,25-tetrol, astramembrangenin, cycloastragenol SMILES CC1(C){3}[C@@H](O)CC[C@]2(C3)C43CC[C@]5(C)[C@@H]([C@](C)(O6)CC[C@H]6{25}C(C)(O)C){16}[C@@H](O)C[C@](C)5[C@]4([H])C{6}[C@H](O)[C@@]12[H] |
Show graphically |
Structure type: oligomer
Trivial name: isoastragaloside I
Compound class: glycoside
- Compound ID: 22360
|
b-D-Glcp-(1-6)-+
|
b-D-Xylp3Ac-(1-3)-Subst
Subst = 20R,24S-epoxy-9,19-cyclolanostane-3β,6α,16β,25-tetrol, astramembrangenin, cycloastragenol SMILES CC1(C){3}[C@@H](O)CC[C@]2(C3)C43CC[C@]5(C)[C@@H]([C@](C)(O6)CC[C@H]6{25}C(C)(O)C){16}[C@@H](O)C[C@](C)5[C@]4([H])C{6}[C@H](O)[C@@]12[H] |
Show graphically |
Structure type: oligomer
Trivial name: isoastragaloside II
Compound class: glycoside
Expand this publication
Collapse this publication
Total list of publication IDs on all result pages of the current query:
Total list of corresponding CSDB IDs (permanent record IDs):
Execution: <1 sec