A series of six isopimarane-type diterpene glycosides, along with an eremophilane-type sesquiterpene, i.e., elaeicolasides A-C (1-3, resp.), 16-(α-D-mannopyranosyloxy)isopimar-7-en-19-oic acid (4), hymatoxin K (5), hymatoxin L (6), and elaeicolalactone (7), were isolated from the AcOEt extract of the fermented broth of Stilbohypoxylon elaeicola YMJ173. Among these, 1-3 and 7 are new compounds based on their spectroscopic data and sugar composition analysis. The effects of 1-7 on the inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-activated murine macrophage RAW264.7 cells were evaluated. All these compounds inhibited NO production, detected as nitrite in the culture medium, in activated macrophages without any cytotoxicity at a concentration of 100 μM. Among these compounds, 2 showed a significant activity with the average maximum inhibition (E(max)) and median inhibitory concentration (IC(50)) values of 93.3±0.5% and 79.3±0.4 μM, respectively.
NCBI PubMed ID: 22253110Publication DOI: 10.1002/cbdv.201100025Journal NLM ID: 101197449Publisher: Verlag Helvetica Chimica Acta
Correspondence: thlee
tmu.edu.tw
Institutions: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei, Taiwan, L5 Research Center, China Medical University Hospital, Taichung, Taiwan, School of Pharmacy, Taipei Medical University, Taipei, Taiwan, Genomics Research Center, Academia Sin, Taipei, Taiwan
Methods: 13C NMR, 1H NMR, IR, TLC, ESI-MS, acid hydrolysis, UV, HPAEC-PAD, extraction, optical rotation measurement, CC, RP-HPLC, cell growth, HR-ESI-MS, determination of NO production, cell viability assay, evaporation
Found