Three zinniol analogues, pleoniols A-C (1-3), together with a known compound (4), were isolated from a mixed fermentation of two endophytic fungi, Pleosporales sp. F46 and Acremonium pilosum F47, both of which are found the pedicel of the medicinal plant Mahonia fortunei. High-performance liquid chromatography analysis showed that none of compounds 1-4 were detected when either one of the two fungi was cultured alone. The structures of compounds 1-4, including the absolute configurations, were deduced based on the interpretation of HRMS, NMR, and optical rotation data. Compounds 1-3 exhibited no antimicrobial efficacies against four Gram-positive or Gram-negative bacteria and two plant pathogenic fungi. Moreover, they demonstrated no obvious cytotoxic activities against four cancer cell lines, A549, MDA-MB-231, CT-26 and MCF-7. Our work indicates that the co-culture of endophytes in the same ecological niche is an effective approach for promoting interspecies interaction and enriching chemical diversity.

endophytes, bioactivity, secondary metabolites, co-cultivation, zinniol analogues

• Compound ID: 22495
  

  Subst1-(1-3)-+ | b-D-Glcp-(1-5)-Subst Subst = 4-(hydroxymethyl)-2,5,6-trimethylbenzene-1,3-diol = SMILES CC1={5}C(O)C(CO)=C(C)C(C)={3}C1O; Subst1 = 3-methylbut-2-en-1-ol = SMILES O{1}C/C=C(C)/C

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  Structure type: monomer ; 435.1981 [M+Na]+
  C21H32O8
  Trivial name: pleoniol A
  Compound class: glycoside
  
   
  
  Pleosporales sp. F46, Acremonium pilosum F47
  CSDB ID 50586 (all data & tools)