Oligo-deoxyadenylic acid (dAX) forms a novel 1:2 triple-helix with β-1,3-D-glucan schizophyllan (SPG). We found that dAX meticulously selects the most suitable length of SPG to bind; for example, dA30 only complexes with a short SPG chain having 30, 60, or 90 main-chain glucoses, and they can be easily isolated with each other. This study demonstrated such a novel stoichiometric complex formation by using gel permeation chromatography coupled with multi-angle light scattering and synchrotron small-angle X-ray scattering. These oligo-DNA/polysaccharide complexes can be used as a tool for delivering therapeutic oligonucleotides to immunocytes that express the β-1,3-D-glucan receptors. The present study provides a robust platform technique to characterize them in terms of modern regulatory science of nanomedicines, which is requisite to transfer drug candidates into clinical trial. Our findings are important for characterizing these complexes as well as for providing a new insight into nucleotide and saccharide chemistry.

binding, schizophyllan, triple-helix, oligo-DNA, delivering, nanomedicine

NCBI PubMed ID: 33186018
Publication DOI: 10.1021/acs.biomac.0c01038
Journal NLM ID: 100892849
Publisher: Washington, DC: American Chemical Society
Correspondence: sakuraikitakyu-u.ac.jp
Institutions: Department of Chemistry and Biochemistry, University of Kitakyushu, Kitakyushu, Japan
Methods: 13C NMR, viscosity measurement, CD, elemental analysis, dialysis, precipitation, sonication, filtration, SAXS, GPC-MALLS, qNMR
 

• Compound ID: 16857
  

  b-D-Glcp-(1-6)-+ | -3)-b-D-Glcp-(1-3)-b-D-Glcp-(1-3)-b-D-Glcp-(1-

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  Structure type: polymer chemical repeating unit
  Trivial name: β-D-glucan, β-glucan, schizophyllan, carboxymethylglucan, pleuran, scleroglucan, lentinan, GRN, SPG, sizofiran, shizophyllan, Scleroglucan, lentinan-type beta-glucans (Ths-2), b-(1-3,6)-glucan, HEP3, grifolan LE, schizophyllan, scleroglucan, lentinan, termitan, grifolan, schizophyllan, scleroglucan, schizophyllan, sizofiran, grifolan, scleroglucan, schizophyllan, sonifilan, schizophyllan, grifolan, G. frondosa polysaccharide (GFP), alkali-soluble β-glucan (PeA3), schizophyllan (SPG), β-glucan, schizophyllan, β-glucan, schizophyllan, scleroglucan, pleuran
  Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide, β-glucan, b-glucan, scleroglucan, D-glucan
  Reference(s) to other database(s): GTC:G66305IS, CCSD:49943, CBank-STR:12679, CA:9050-67-3
  
   
  
  Schizophyllum commune
  CSDB ID 50669 (all data & tools)