A novel oligosaccharide was isolated and identified from the lipooligosaccharide fraction of the halophilic marine bacterium Arcobacter halophilus. The complete structure was achieved by chemical analysis, 2D NMR spectroscopy, and MALDI mass spectrometry as the following: α-Glc-(1→7)-α-Hep-(1→5)-α-Kdo4P-(2→6)-β-GlcN4P-(1→6)-α-GlcN1P.
oligosaccharide structure, Lipooligosaccharide, NMR spectroscopy, Marine bacteria, Arcobacter halophilus
NCBI PubMed ID: 20149349Publication DOI: 10.1016/j.carres.2010.01.012Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: A. Molinaro
Institutions: Dipartimento di Chimica Organica e Biochimica, Universita di Napoli Federico II, via Cintia 4, Naples, Italy
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, HF solvolysis, SDS-PAGE, sugar analysis, 31P NMR, GLC, alkaline degradation, MALDI-TOF MS, NMR-1D
Found