The capsular polysaccharide (CPS) of Streptococcus suis serotype 2 was isolated, purified, chemically modified, and characterized. Sugar and absolute configuration analyses of the CPS gave the following composition: D-Gal, 3; D-Glc, 1; D-GlcNAc, 1; D-Neu5Ac, 1; L-Rha, 1. Sialic acid was found to be terminal, and the CPS was quantitatively desialylated by mild acid hydrolysis. The CPS was also submitted to periodate oxidation followed by borohydride reduction and Smith degradation. Sugar and methylation analysis, 1H and 13C nuclear magnetic resonance, and mass spectrometry of the native CPS or of its specifically modified products allowed to determine the repeating unit sequence: [4)[Neu5Ac(α2-6)Gal(β1-4)GlcNAc(β1-3)]Gal(β1-4)[Gal(α1-3) ]Rha(β1-4)Glc(β1-]n. The backbone sequence was found to be identical to that of Streptococcus agalactiae or group B Streptococcus (GBS) type VIII and Streptococcus pneumoniae type 23F. The S. suis CPS shares the sequence Neu5Ac-Gal-GlcNAc-Gal in common with GBS types Ia, Ib, II, III, and IV CPSs but differs from them by the presence of rhamnose and the fact that sialic acid is 2,6- rather than 2,3-linked to the following Gal. A correlation between the S. suis CPS sequence and genes of the serotype 2 cps locus encoding putative enzymes responsible for the biosynthesis of the repeating unit was tentatively established.
chemical modification, nuclear magnetic resonance (NMR), mass spectrometry (MS), repeating unit sequence
NCBI PubMed ID: 20555393Publication DOI: 10.1139/O09-170Journal NLM ID: 8606068Publisher: Ottawa: National Research Council of Canada
Correspondence: marie-rose.vancalsteren@agr.gc.ca
Institutions: Centre de recherche et de developpement sur les aliments, Agriculture et Agroalimentaire Canada, Saint-Hyacinthe, QC J2S 8E3, Canada, Groupe de recherche sur les maladies infectieuses du porc (GREMIP) and Centre de recherche en infectiologie porcine (CRIP), Faculté de médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, QC J2S 2M2, Canada
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, ESI-MS, mild acid hydrolysis, GC, Smith degradation, NMR-1D, methanolysis, SEC-MALS
Found