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1. (Article ID: 9391)
Li H, Xie W, Sun H, Cao K, Yang X
Effect of the structural characterization of the fungal polysaccharides on their immunomodulatory activity
International Journal of Biological Macromolecules 164 (2020)
3603-3610
The immunomodulatory effects of the four extracellular polysaccharides, namely WPA, WPB, AP2A, and TP1A, which were isolated from the fermented broth of Aspergillus aculeatus, A. terreus and Trichoderma sp. KK19L1, were investigated in vitro. WPA, WPB, AP2A, and TP1A were not toxic to RAW264.7 cells. These polysaccharides enhanced cell viability. WPA, WPB, AP2A, and TP1A showed increased immunomodulatory effect by strengthening the phagocytic activity and enhancing the release of NO, TNF-α and IL-6 from RAW264.7 cells. WPA, WPB, AP2A, and TP1A exhibited different immunomodulatory activity in vitro due to their different structural characterizations, and their immunoregulatory effects decreased successively in the following order: WPA, WPB, AP2A, and TP1A. The extracellular polysaccharides WPA, WPB, AP2A, and TP1A had potent immunomodulatory effects and could be used as potential immunomodulatory agents in the fields of functional food and medicine.
extracellular polysaccharide, immunomodulatory activity, Structural characterization, fungus
NCBI PubMed ID: 32860795Publication DOI: 10.1016/j.ijbiomac.2020.08.189Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: yangxihong63
163.com
Institutions: College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Shandong, China, Shandong Provincial Key Laboratory of Biochemical Engineering, Shandong, China, Qingdao Academy of Agricultural Sciences, Shandong, China, College of Electromechanical Engineering, Qingdao University of Science and Technology, Shandong, China
Methods: 13C NMR, 1H NMR, ELISA, biological assays, ion-exchange chromatography, extraction, optical rotation measurement, cell growth, determination of NO production, precipitation, SEM, Congo Red assay
The publication contains the following compound(s):
- Compound ID: 22730
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a-D-Glcp-(1-?)-+
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a-D-Glcp-(1-?)-+ |
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a-D-Glcp-(1-2)-+ | |
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a-D-Glcp-(1-2)-+ | | |
| | | |
a-D-Manp-(1-6)-a-D-Glcp-(1-6)-a-D-Glcp-(1-6)-+ a-D-Glcp-(1-2)-+ | | | |
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-4)-a-D-Glcp-(1-6)-b-D-Galf-(1-6)-b-D-Galf-(1-6)-b-D-Galf-(1-6)-b-D-Galf-(1-6)-b-D-Galf-(1-?)-a-D-Manp-(1-?)-a-D-Manp-(1- |
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Structure type: structural motif or average structure
; 40000
Compound class: polysaccharide, glucogalactomannan
- Compound ID: 22728
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a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Manp-(1-2)-+
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-5)-b-D-Galf-(1-6)-a-D-Manp-(1- |
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Structure type: structural motif or average structure
; 28100
Compound class: polysaccharide, galactomannan
Reference(s) to other database(s): GenDB:JX501394
- Compound ID: 22729
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a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Manp-(1-2)-+
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-6)-a-D-Manp-(1- |
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Structure type: structural motif or average structure
; 21000
Compound class: polysaccharide, mannan
Reference(s) to other database(s): GenDB:JX501394
- Compound ID: 22727
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66%a-D-Manp-(1-2)-50%a-D-Manp-(1-2)-+
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66%a-D-Manp-(1-2)-50%a-D-Manp-(1-2)-+ |
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-3)-a-D-Manp-(1-3)-a-D-Manp-(1-6)-{{{-a-D-Manp-(1-6)-}}}/n=2/-a-D-Manp-(1- |
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Structure type: structural motif or average structure
; 30300
Compound class: polysaccharide, mannan
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2. (Article ID: 9392)
Li H, Gao T, Wang J, Tian S, Yuan X, Zhu H
Structural identification and antitumor activity of the extracellular polysaccharide from Aspergillus terreus
Process Biochemistry 51(10) (2016)
1714-1720
A water-soluble extracellular polysaccharide, AP2A, was purified from the culture medium of Aspergillus terreus. AP2A was mainly composed of mannose with the average molecular weight of about 30.3 kDa. By chemical, IR, GC/MS, 1D and 2D NMR spectroscopic analysis, the backbone of AP2A was found to be composed of [→3)-α-D-Manp(1→], [→6)-α-D-Manp(1→] and [→2,6)-α-D-Manp(1→] with [α-D-Manp-(1→2)-α-D-Manp(1→] and [α-D-Manp-(1→] as branches substituted at the C-2 position of [→2,6)-α-D-Manp(1→]. AP2A showed an anti-proliferative effect in vitro on HeLa and MCF-7 cells in a dose- and time-dependent manner. Flow cytometry showed that AP2A inhibited HeLa cell proliferation by arresting cell cycle in G2/M phase, and Annexin V-FITC/PI double staining assay indicated that AP2A could also induce HeLa cells apoptosis. These results suggest that the extracellular polysaccharide, AP2A from Aspergillus terreus, has possible chemotherapeutic applications.
structure, extracellular polysaccharide, antitumor activity, Aspergillus terreus
Publication DOI: 10.1016/j.procbio.2016.08.001Journal NLM ID: 9211419Publisher: Barking, Essex: Elsevier Applied Science
Correspondence: Li H
163.com>; Zhu H hbu.edu.cn>
Institutions: College of Chemistry and Environment Science, Hebei University, Baoding, China, College of Pharmaceutical Science, Hebei University, Baoding, China, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, Baoding, China
Methods: 13C NMR, 1H NMR, methylation, IR, GC-MS, acid hydrolysis, biological assays, HPLC, optical rotation measurement, acetylation, reduction, CC, cell growth, flow cytometry analysis, HPGPC, precipitation, phenol-sulfuric acid assay, derivatization, Bradford method, evaporation, MTT, carbazole method, filtration, cell proliferation assay, apoptosis assay, staining
The publication contains the following compound(s):
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