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Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_133754,IEDB_136105,IEDB_142488,IEDB_146664,IEDB_2116141,IEDB_225177,IEDB_885823,IEDB_983931,SB_192

• Article ID: 4016
  Fedonenko YP, Boyko AS, Zdorovenko EL, Konnova SA, Shashkov AS, Ignatov VV, Knirel YA "Structural pecularities of the O-specific polysaccharides of Azospirillum bacteria of serogroup III" - Biochemistry (Moscow) 76(7) (2011) 797-802
  

  Azospirillum brasilense SR55, isolated from the rhizosphere of Triticum durum, was classified as serogroup II on the basis of serological tests. Such serogroup affiliation is uncharacteristic of wheat-associated Azospirillum species. The lipid A of A. brasilense SR55 lipopolysaccharide contained 3-hydroxytetradecanoic, 3-hydroxyhexadecanoic, hexadecanoic and octadecenoic fatty acids. The structure of the lipopolysaccharide's O polysaccharide was established, with the branched octasaccharide repeating unit being represented by l-rhamnose, l-3-O-Me-rhamnose, d-galactose and d-glucuronic acid. The SR55 lipopolysaccharide induced deformations of wheat root hairs. The lipopolysaccharide was not involved in bacterial cell aggregation, but its use to pretreat wheat roots was conducive to cell adsorption. This study shows that Azospirillum bacteria can utilise their own lipopolysaccharide as a carbon source, which may give them an advantage in competitive natural environments.

  Lipopolysaccharide, O-antigen, O-Polysaccharide structure, serological characterization, Azospirillum

  NCBI PubMed ID: 21999541
  Publication DOI: 10.1134/S0006297911070108
  Journal NLM ID: 0376536
  Publisher: Nauka/Interperiodica
  Correspondence: room308@ibppm.sgu.ru
  Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Laboratory of Biochemistry, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov 410049, Russia, Saratov State University, ul. Astrakhanskaya 83, 410012 Saratov, Russia
  Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, SDS-PAGE, sugar analysis, acid hydrolysis, GLC
  
   
  
  Azospirillum brasilense S54
  CSDB ID 26618 (all data & tools)
• Article ID: 4723
  Sigida EN, Fedonenko YP, Shashkov AS, Grinev VS, Zdorovenko EL, Konnova SA, Ignatov VV, Knirel YA "Structural studies of the polysaccharides from the lipopolysaccharides of Azospirillum brasilense Sp246 and SpBr14" - Carbohydrate Research 398 (2014) 40-44
  

  Lipopolysaccharides from closely related Azospirillum brasilense strains, Sp246 and SpBr14, were obtained by phenol-water extraction. Mild acid hydrolysis of the lipopolysaccharides followed by GPC on Sephadex G-50 resulted in polysaccharide mixtures. On the basis of sugar and methylation analyses, Smith degradation and (1)H and (13)C NMR spectroscopy data, it was concluded that both bacteria possess the same two distinct polysaccharides having structures 1 and 2: [structure: see text]. Structure 1 has been reported earlier for a polysaccharide of A. brasilense 54 [Fedonenko et al., 2011] whereas to our knowledge structure 2 has not been hitherto found in bacterial polysaccharides.

  Lipopolysaccharide, Azospirillum brasilense, bacterial polysaccharide structure

  NCBI PubMed ID: 25240180
  Publication DOI: 10.1016/j.carres.2014.05.008
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: Sigida EN
  Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Prospekt Entuziastov 13, 410049 Saratov, Russia, Chernyshevsky Saratov State University, Russia
  Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, GLC, mild acid hydrolysis, Smith degradation, composition analysis, GPC
  
   
  
  Azospirillum brasilense Sp246, Azospirillum brasilense 54
  CSDB ID 30132 (all data & tools)
• Article ID: 4844
  Fedonenko YP, Sigida EN, Konnova SA, Ignatov VV "Structure and serology of O-antigens of nitrogen-fixing rhizobacteria of the genus Azospirillum" - Russian Chemical Bulletin = Izvestiia Akademii nauk. Seriia khimicheskaia 64(5) (2015) 1024-1031
  

  The review presents a discussion of the published data of 2002 to 2014 dealing with structural studies of O-specific polysaccharides, representatives of the Azospirillum diazotrophic rhizobacteria, summarizing the results of a series of authors? studies. Some general structures incorporated in O-specific polysaccharides responsible for classification of bacterial cultures into serogroups on the basis of immunochemical studies were characterized. The structures of O-specific polysaccharides of seven azospirillum strains identical to those of previously studied strains are presented in this review for the first time.

  lipopolysaccharides, O-specific polysaccharides, Structures, Azospirillum, O-antigenic determinants

  Publication DOI: 10.1007/s11172-015-0971-x
  Journal NLM ID: 100912060
  Publisher: New York: Consultants Bureau
  Correspondence: si_elena@mail.ru
  Institutions: Institute of Biochemistry and Physiology of Plants and Microorganisms Russian Academy of Sciences, 13 prosp. Entuziastov, 410049 Saratov, Russian Federation
  
   
  
  Azospirillum brasilense 54, Azospirillum brasilense SR8, Azospirillum brasilense Sp246, Azospirillum brasilense SpBr14
  CSDB ID 11174 (all data & tools)
• Article ID: 5498
  Sigida EN, Fedonenko YP, Shashkov AS, Toukach PV, Shelud'ko AV, Zdorovenko EL, Knirel YA, Konnova SA "Structural studies of O-specific polysaccharide(s) and biological activity toward plants of the lipopolysaccharide from Azospirillum brasilense SR8" - International Journal of Biological Macromolecules 126 (2019) 246-253
  

  Lipopolysaccharide (LPS) was extracted from dry bacterial cells of plant-growth-promoting bacterium Azospirillum brasilense SR8 (IBPPM 5). The O-specific polysaccharide (OPS) was obtained by mild acid hydrolysis of the lipopolysaccharide and studied by sugar analysis, 1H and 13C NMR spectroscopy, including 1H,1H COSY, TOCSY, ROESY, and 1H,13C HSQC and HMBC experiments, computational NMR-based structure analysis, and Smith degradation. The OPS was shown to contain two types of repeating units of the following structure: Both OPS structures are present in A. brasilense 54, from which structure 1 has been reported earlier (Fedonenko et al., 2011), whereas to our knowledge structure 2 has not been hitherto found in bacterial saccharides. Treatment of wheat seedling roots with LPS of A. brasilense SR8 increased the number of root hair deformations as compared to seedlings grown without LPS, but had no effect on adsorption of the bacteria to the root surface. A. brasilense SR8 was able to utilize LPS of several structurally related Azospirillum strains.

  Lipopolysaccharide, structure, O-specific polysaccharide, Azospirillum brasilense, Chemotaxis, NMR simulation, Root hair deformation

  NCBI PubMed ID: 30590146
  Publication DOI: 10.1016/j.ijbiomac.2018.12.229
  Journal NLM ID: 7909578
  Publisher: Butterworth-Heinemann
  Correspondence: E.N. Sigida
  Institutions: Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospect Entuziastov, Saratov 410049, Russia, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 47 Leninsky Prospect, Moscow, Russia, N. G. Chernyshevsky Saratov State University, 83 Ulitsa Astrakhanskaya, Saratov 410012, Russia
  Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, de-O-acylation, SDS-PAGE, sugar analysis, acid hydrolysis, GLC, GC, Smith degradation, GPC, 13C NMR analysis by GRASS, experiments on plants
  
   
  
  Azospirillum brasilense SR8 (IBPPM 5), Azospirillum brasilense 54, Azospirillum brasilense 246, Azospirillum brasilense SpBr14
  CSDB ID 1108 (all data & tools)

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Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_130648,IEDB_136105,IEDB_137473,IEDB_225177,IEDB_885813,IEDB_885823

• Article ID: 4844
  Fedonenko YP, Sigida EN, Konnova SA, Ignatov VV "Structure and serology of O-antigens of nitrogen-fixing rhizobacteria of the genus Azospirillum" - Russian Chemical Bulletin = Izvestiia Akademii nauk. Seriia khimicheskaia 64(5) (2015) 1024-1031
  

  The review presents a discussion of the published data of 2002 to 2014 dealing with structural studies of O-specific polysaccharides, representatives of the Azospirillum diazotrophic rhizobacteria, summarizing the results of a series of authors? studies. Some general structures incorporated in O-specific polysaccharides responsible for classification of bacterial cultures into serogroups on the basis of immunochemical studies were characterized. The structures of O-specific polysaccharides of seven azospirillum strains identical to those of previously studied strains are presented in this review for the first time.

  lipopolysaccharides, O-specific polysaccharides, Structures, Azospirillum, O-antigenic determinants

  Publication DOI: 10.1007/s11172-015-0971-x
  Journal NLM ID: 100912060
  Publisher: New York: Consultants Bureau
  Correspondence: si_elena@mail.ru
  Institutions: Institute of Biochemistry and Physiology of Plants and Microorganisms Russian Academy of Sciences, 13 prosp. Entuziastov, 410049 Saratov, Russian Federation
  
   
  
  Azospirillum brasilense 54, Azospirillum brasilense SR8
  CSDB ID 11175 (all data & tools)