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1. (Article ID: 95)
Gamian A, Katzenellenbogen E, Romanowska E, Grosskurth H, Dabrowski J
Reinvestigation of the O-specific polysaccharides of Hafnia alvei lipopolysaccharides isolated from strains ATCC 13337 and 1187
Carbohydrate Research 307(1-2) (1998)
173-176
The structure of the O-specific polysaccharides of the lipopolysaccharides produced by Hafnia alvei strains ATCC 13337 and 1187 was reinvestigated. The position of phosphate group in the repeating units of the polysaccharides was established with the aid of 1H detected, 31P edited NMR spectra. According to the results obtained, the polysaccharides are teichoic acid-like polymers with the repeating units of the following structure: [formula: see text] where Acyl = D-3-hydroxylbutyryl, and 3-O-acetylation was approximately 30%
Lipopolysaccharide, O-specific polysaccharide, Hafnia alvei
NCBI PubMed ID: 9658571Publication DOI: 10.1016/S0008-6215(98)00035-4Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: gamian
immuno.iitd.pan.wroc.pl
Institutions: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Wroclaw, Poland
Methods: methylation, NMR-2D, FAB-MS, NMR, sugar analysis, Smith degradation
The publication contains the following compound(s):
- Compound ID: 338
|
R-3HOBut-(1-2)-+ a-D-Glcp-(1-6)-+
| |
-6)-a-D-GlcpN3(30%)Ac-(1-4)-a-D-GalpNAc-(1-3)-b-D-GalpNAc-(1-2)-a-D-Glcp-(1-P- |
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Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide, O-antigen
- Compound ID: 339
|
R-3HOBut-(1-2)-+
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-2)-a-D-Glcp-(1--P--6)--a-D-GlcpN-(1-4)-a-D-GalpNAc-(1-3)-b-D-GalpNAc-(1- |
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Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide, O-antigen
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2. (Article ID: 130)
Tuinier R, van Casteren WH, Looijesteijn PJ, Schols HA, Voragen AG, Zoon P
Effects of structural modifications on some physical characteristics of exopolysaccharides from Lactococcus lactis
Biopolymers 59(3) (2001)
160-166
The relationship between the primary structure and the chain stiffness of exopolysaccharides (EPSs) and modified EPSs produced by two strains of Lactococcus lactis subsp. cremoris was investigated. The molar mass and radius of gyration of these exopolysaccharides were analyzed by multiangle static light scattering after size-exclusion chromatography. From these results and the chemical structure of the repeating units of the investigated EPSs, the Kuhn lengths could be calculated. We found that the initial Kuhn lengths of the two native EPSs are similar. Modification of the EPSs by removing parts of the side groups resulted in a decrease in both the absolute value and the normalized value of the Kuhn length. It is therefore concluded that partial removal of the side groups of these polysaccharides could make them less efficient as thickeners if no specific interaction with other components occurs.
exopolysaccharide, chemical structure, light scattering, interaction, Lactococcus lactis, size-exclusion chromatography, Kuhn lenth
NCBI PubMed ID: 11391565Publication DOI: 10.1002/1097-0282(200109)59:3<160::AID-BIP1015>3.0.CO;2-VJournal NLM ID: 0372525Publisher: Wiley Interscience
Correspondence: r.tuinier
chem.uu.nl
Institutions: NIZO food research, P. O. Box 20, 6710 BA Ede, The Netherlands, Laboratory of Food Chemistry, Department of grotechnology and Food Sciences, Wageningen University, Bomenweg 2, 6700 EV Wageningen, The Netherlands, Van't Hoff Laboratory, Debye Research Institute, University of Utrecht, Utrecht, The Netherlands
Methods: SEC-MALS
The publication contains the following compound(s):
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