Natural products that inhibit cell cycle progression may have potential as anticancer agents. In this study, cell cycle inhibition of microbial culture extracts was screened by fluorescent images using HeLa/Fucci2 cells. The culture extract of a fungus, Akanthomyces sp., inhibited the cell cycle progression at the S/G2/M phases, and bioassay-guided fractionation of the extract afforded three previously undescribed aphidicolin derivatives, namely akanthomins A-C, and an undescribed chromone glycoside, specifically 9-hydroxyeugenetin 9-O-β-d-(4-O-methyl)glucopyranoside, in addition to aphidicolin. The chemical structures of these compounds were elucidated by spectroscopic analysis and chemical derivatization. Using a flow cytometer, akanthomin A and aphidicolin were found to inhibit cell cycle progression at the S phase.

pharmaceutical, fungus, cordycipitaceae, Akanthomyces sp., Aphidicolin derivative, cell cycle inhibitor

• Compound ID: 23469
  

  b-D-Glcp4Me-(1-9)-Subst Subst = 9-hydroxyeugenetin = SMILES O{9}CC(OC1=CC(OC)=C(C){5}C(O)=C12)=CC2=O

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  Structure type: monomer
  C19H24O10
  Trivial name: chromone glycoside, 9-hydroxyeugenetin 9-O-β-d-(4-O-methyl)glucopyranoside
  
   
  
  Akanthomyces sp. 18F18403
  CSDB ID 41044 (all data & tools)