Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Publication DOI: 10.1007/s11172-019-2432-4Journal NLM ID: 100912060Publisher: New York: Consultants Bureau
Correspondence: YA Knirel <yknirel
gmail.com>
Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia, Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health Queensland University of Technology, Brisbane, Australia, M. M. Shemyakin-Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia, Moscow Institute of Physics and Technology, 9 Institutskii per., Russian Federation, State Research Center for Applied Microbiology and Biotechnology, Obolensk, Russian Federation
The K5 capsular polysaccharide (CPS) was isolated from the bacterium Acinetobacter baumannii (A. baumannii) SDF and studied by 1D and 2D 1H and 13C NMR spectroscopy before and after O-deacetylation and partial acid hydrolysis. The CPS was found to be composed of branched tetrasaccharide repeats (K units) containing 5,7-diacetamido-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic acid (Leg5Ac7Ac). The KL5 capsule biosynthesis gene cluster at the K locus is consistent with the composition and structure of the CPS. KL5 is almost identical to the KL7 gene ulosonic acid (Leg5Ac7Ac A. baumannii LUH5533 that has been characterized earlier, and differs only in the gene for Wzy polymerase that is responsible for the formation of the linkage between the K units. The K5 CPS from strain SDF and the K7 CPS from strain LUH5533 have the same K-unit structure but a different linkage between the K units, which is formed by distinct Wzy polymerases. As opposite to Leg5Ac7Ac in the K7 CPS, this monosaccharide is O-acetylated at position 4 in the K5 CPS. No acetyltransferase for this modification of the K5 CPS is present in the KL5 gene cluster, and hence it is encoded by a gene located elsewhere in the genome.
Acinetobacter baumannii, capsular polysaccharide, legionaminic acid, Wzy polymerase, gene polymorphism, KL gene locus
Structure type: polymer chemical repeating unit
Location inside paper: p.164, Scheme 1, K7
Compound class: CPS
Contained glycoepitopes: IEDB_130648,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_141807,IEDB_151528,IEDB_151531,IEDB_190606,SB_7
Methods: 13C NMR, 1H NMR, NMR-2D, partial acid hydrolysis, sugar analysis, GLC, de-O-acetylation, bioinformatic analysis
Biosynthesis and genetic data: Gtr13,Gtr14, Gtr15, Wzy(K7)[ItrA2]
Related record ID(s): 1180, 1181, 1182
NCBI Taxonomy refs (TaxIDs): 470Reference(s) to other database(s): GTC:G59392AM
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There is only one chemically distinct structure:
Found 
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