A sulfated galactoglucan (3-SS) was discovered in Antrodia cinnamomea with antiproliferative and anti-inflammatory activities. Chemical identification of 3-SS resulted in the determination of a partial repeat unit as a 2-O sulfated 1,3-/1,4-linked galactoglucan with a two-residual 1,6-O-β-Glc branch on the 3-O position of a Glc, by monosaccharide analysis and 1D and 2D NMR spectroscopy. The anti-inflammation effects of 3-SS on RAW264.7 macrophage cells, such as IL-6 inhibition, restoration of LPS-induced IκB protein degradation, and inhibited LPS-induced TGFRII protein degradation, were confirmed to occur via AKT, ERK1/2, and p-38. In addition, 3-SS impaired the proliferation of H1975 lung cancer cells through EGFR/ERK/slug signaling. This is the first finding of 2-O sulfated 1,3-/1,4-galactoglucan with 1,6-β-Glc branches with dual functions of anti-inflammatory and antiproliferative activities.

sulfated polysaccharides, Anti-inflammatory effects, Antrodia cinnamomea, antiproliferation

• Compound ID: 23525
  

  S-2)-+ | S-2)-b-D-Glcp-(1-6)-b-D-Glcp-(1-3)-+ | S-2)-+ S-2)-+ S-2)-+ | | | | | -3)-b-D-Glcp-(1-3)-a-D-Galp-(1-4)-b-D-Glcp-(1-4)-a-D-Glcp-(1- | S-2)-+

  Show graphically

  Show legend Show as text

  Structure type: structural motif or average structure
  Trivial name: sulfated galactoglucan (3-SS)
  
   
  
  Antrodia cinnamomea B86
  CSDB ID 41067 (all data & tools)