Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: cystic fibrosis (CF) [ICD11:
CA25 
]
NCBI PubMed ID: 31229548Publication DOI: 10.1016/j.ijbiomac.2019.06.140Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: pcescutti
units.it
Institutions: Department of Life Sciences, University of Trieste, Via Licio Giorgieri 1, 34127 Trieste, Italy, Department of Chemical and Pharmaceutical Sciences, University of Trieste, Via Licio Giorgieri 1, 34127 Trieste, Italy, Department of Food Sciences, Cornell University, M10 Stocking Hall, Ithaca, NY 14853-5701, USA
Microorganisms often grow in communities called biofilms where cells are imbedded in a complex self-produced biopolymeric matrix composed mainly of polysaccharides, proteins, and DNA. This matrix, together with cell proximity, confers many advantages to these microbial communities, but also constitutes a serious concern when biofilms develop in human tissues or on implanted prostheses. Although polysaccharides are considered the main constituents of the matrices, their specific role needs to be clarified. We have investigated the chemical and morphological properties of the polysaccharide extracted from biofilms produced by the C1576 reference strain of the opportunistic pathogen Burkholderia multivorans, which causes lung infections in cystic fibrosis patients. The aim of the present study is the definition of possible interactions of the polysaccharide and the three-dimensional conformation of its chain within the biofilm matrix. Surface plasmon resonance experiments confirmed the ability of the polysaccharide to bind hydrophobic molecules, due to the presence of rhamnose dimers in its primary structure. In addition, atomic force microscopy studies evidenced an extremely compact three-dimensional structure of the polysaccharide which may form aggregates, suggesting a novel view of its structural role into the biofilm matrix.
conformation, polysaccharide, Biofilm, AFM, Burkholderia multivorans, SPR, Molecular interactions, Polysaccharide functions
Structure type: polymer chemical repeating unit
Location inside paper: p.945, fig.1
Trivial name: rhamno-mannan, rhamnomannan, Epol C1576
Compound class: EPS
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_1394181,IEDB_143632,IEDB_144983,IEDB_145010,IEDB_152206,IEDB_983930,SB_136,SB_196,SB_44,SB_67,SB_72
Methods: SPS, atomic force microscopy (AEM)
Comments, role: published polymerization frame was shifted for conformity with other records
3D data: 3D data
NCBI Taxonomy refs (TaxIDs): 87883Reference(s) to other database(s): GTC:G72472ZR
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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