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Liu MA, Kidambi A, Reeves PR
The low level of O antigen in Salmonella enterica Paratyphi A is due to inefficiency of the glycosyltransferase WbaV
FEMS Microbiology Letters 368(3) (2021)
fnab009
|
a-Tyvp-(1-3)-+
|
-2)-a-D-Manp-(1-4)-a-L-Rhap-(1-3)-a-D-Galp-(1- |
Show graphically |
Salmonella enterica D1
(Ancestor NCBI TaxID 28901,
species name lookup)
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: infection due to Salmonella enterica [ICD11:
XN5VC 
]
NCBI PubMed ID: 33476372Publication DOI: 10.1093/femsle/fnab009Journal NLM ID: 7705721Publisher: Blackwell Publishing
Correspondence: peter.reeves
sydney.edu.au
Institutions: School of Life and Environmental Sciences, The University of Sydney, NSW 2006, Australia
The group A O antigen is the major surface polysaccharide of Salmonella enterica serovar Paratyphi A (SPA), and the focal point for most current vaccine development efforts. The SPA O-antigen repeat (O unit) is structurally similar to the group D1 O unit of S. enterica serovar Typhi, differing only in the presence of a terminal side-branch paratose (Par) in place of tyvelose (Tyv), both of which are attached by the glycosyltransferase WbaV. The two O-antigen gene clusters are also highly similar, but with a loss-of-function mutation in the group A tyv gene and the tandem amplification of wbaV in most SPA strains. In this study, we show that SPA strains consistently produce less O antigen than their group D1 counterparts and use an artificial group A strain (D1 Deltatyv) to show this is due to inefficient Par attachment by WbaV. We also demonstrate that group A O-antigen production can be increased by overexpression of the wbaV gene in both the D1 Deltatyv strain and two multi-wbaV SPA strains. These findings should be broadly applicable in ongoing vaccine development pipelines, where efficient isolation and purification of large quantities of O antigen is of critical importance
biosynthesis, O antigen, glycosyltransferase, paratose, Paratyphi A, WbaV
Structure type: polymer chemical repeating unit
Location inside paper: Fig. 1A, group D1
Compound class: O-polysaccharide, O-antigen, CPS
Contained glycoepitopes: IEDB_130660,IEDB_130701,IEDB_136105,IEDB_136779,IEDB_136906,IEDB_137472,IEDB_139421,IEDB_141794,IEDB_144983,IEDB_151528,IEDB_152206,IEDB_174033,IEDB_174035,IEDB_190606,IEDB_225177,IEDB_885823,IEDB_983930,SB_44,SB_67,SB_7,SB_72
Methods: PCR, SDS-PAGE, genetic methods, extraction, growth assays
Enzymes that release or process the structure: WbaV, WbaU, WbaN
Related record ID(s): 10898
NCBI Taxonomy refs (TaxIDs): 28901Reference(s) to other database(s): GTC:G63488DE, GlycomeDB:
3507
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There is only one chemically distinct structure:
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Liu MA, Kidambi A, Reeves PR
The low level of O antigen in Salmonella enterica Paratyphi A is due to inefficiency of the glycosyltransferase WbaV
FEMS Microbiology Letters 368(3) (2021)
fnab009
|
a-Parp-(1-3)-+
|
-2)-a-D-Manp-(1-4)-a-L-Rhap-(1-3)-a-D-Galp-(1- |
Show graphically |
Salmonella enterica ssp. enterica sv. Paratyphi A
(NCBI TaxID 54388,
species name lookup)
Salmonella enterica A
(Ancestor NCBI TaxID 28901,
species name lookup)
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: infection due to Salmonella enterica [ICD11:
XN5VC ]
NCBI PubMed ID: 33476372Publication DOI: 10.1093/femsle/fnab009Journal NLM ID: 7705721Publisher: Blackwell Publishing
Correspondence: peter.reeves
sydney.edu.au
Institutions: School of Life and Environmental Sciences, The University of Sydney, NSW 2006, Australia
The group A O antigen is the major surface polysaccharide of Salmonella enterica serovar Paratyphi A (SPA), and the focal point for most current vaccine development efforts. The SPA O-antigen repeat (O unit) is structurally similar to the group D1 O unit of S. enterica serovar Typhi, differing only in the presence of a terminal side-branch paratose (Par) in place of tyvelose (Tyv), both of which are attached by the glycosyltransferase WbaV. The two O-antigen gene clusters are also highly similar, but with a loss-of-function mutation in the group A tyv gene and the tandem amplification of wbaV in most SPA strains. In this study, we show that SPA strains consistently produce less O antigen than their group D1 counterparts and use an artificial group A strain (D1 Deltatyv) to show this is due to inefficient Par attachment by WbaV. We also demonstrate that group A O-antigen production can be increased by overexpression of the wbaV gene in both the D1 Deltatyv strain and two multi-wbaV SPA strains. These findings should be broadly applicable in ongoing vaccine development pipelines, where efficient isolation and purification of large quantities of O antigen is of critical importance
biosynthesis, O antigen, glycosyltransferase, paratose, Paratyphi A, WbaV
Structure type: polymer chemical repeating unit
Location inside paper: Fig. 1A, group A
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130701,IEDB_136035,IEDB_136105,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_144983,IEDB_151528,IEDB_152206,IEDB_174034,IEDB_190606,IEDB_225177,IEDB_885823,IEDB_983930,SB_44,SB_67,SB_7,SB_72
Methods: PCR, SDS-PAGE, genetic methods, extraction, growth assays
Enzymes that release or process the structure: WbaV, WbaU, WbaN
Related record ID(s): 9691
NCBI Taxonomy refs (TaxIDs): 54388,
28901Reference(s) to other database(s): GTC:G37642RX, GlycomeDB:
27020
Show glycosyltransferases
There is only one chemically distinct structure:
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