Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Shigella flexneri [ICD11:
XN7Y2 
]
The structure was elucidated in this paperNCBI PubMed ID: 34830042Publication DOI: 10.3390/ijms222212160Journal NLM ID: 101092791Publisher: Basel, Switzerland: MDPI
Correspondence: carlo.x.giannelli
gsk.com
Institutions: GSK Vaccines Institute for Global Health (GVGH) S.r.l., via Fiorentina 1, 53100 Siena, Italy, Merck, Via Einaudi 11, 00012 Guidonia, Italy
Shigella is a leading diarrheal cause of morbidity and mortality worldwide, especially in low- and middle-income countries and in children under five years of age. Increasing levels of antimicrobial resistance make vaccine development an even higher global health priority. S. flexneri serotype 6 is one of the targets of many multicomponent vaccines in development to ensure broad protection against Shigella. The O-antigen (OAg) is a key active ingredient and its content is a critical quality attribute for vaccine release in order to monitor their stability and to ensure appropriate immune response. Here, the optimization of two methods to quantify S. flexneri 6 OAg is reported together with the characterization of their performances. The optimized Dische colorimetric method allows a tenfold increment of the sensitivity with respect to the original method and is useful for fast analysis detecting selectively methyl-pentoses, as rhamnose in S. flexneri 6 OAg. Also, a more specific HPAEC-PAD method was developed, detecting the dimer galacturonic acid-galactosamine (GalA-GalN) coming from S. flexneri 6 OAg acid hydrolysis. These methods will facilitate characterization of S. flexneri 6 OAg based vaccines. The colorimetric method can be used for quantification of other polysaccharide containing methyl-pentoses, and the HPAEC-PAD could be extended to other polysaccharides containing uronic acids.
polysaccharide, galacturonic acid, vaccine, quantification, HPAEC-PAD, design of experiment, Dische colorimetric method, methyl-pentose, S.flexneri O-antigen
Structure type: oligomer ; 356.1181 [M+H]+
C
12H
22NO
11Location inside paper: Fig. 3
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_137473
Methods: 13C NMR, 1H NMR, NMR-2D, ESI-MS, HPAEC-PAD, statistical analysis, HPLC-SEC, TFA/HCl hydrolysis, Dische colorimetric method
Comments, role: dimer obtained after TFA hydrolysis of S. flexner 6 OAg.
Related record ID(s): 10927
NCBI Taxonomy refs (TaxIDs): 424719
Show glycosyltransferases
There is only one chemically distinct structure:
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Shigella flexneri [ICD11:
XN7Y2 ]
NCBI PubMed ID: 34830042Publication DOI: 10.3390/ijms222212160Journal NLM ID: 101092791Publisher: Basel, Switzerland: MDPI
Correspondence: carlo.x.giannelli
gsk.com
Institutions: GSK Vaccines Institute for Global Health (GVGH) S.r.l., via Fiorentina 1, 53100 Siena, Italy, Merck, Via Einaudi 11, 00012 Guidonia, Italy
Shigella is a leading diarrheal cause of morbidity and mortality worldwide, especially in low- and middle-income countries and in children under five years of age. Increasing levels of antimicrobial resistance make vaccine development an even higher global health priority. S. flexneri serotype 6 is one of the targets of many multicomponent vaccines in development to ensure broad protection against Shigella. The O-antigen (OAg) is a key active ingredient and its content is a critical quality attribute for vaccine release in order to monitor their stability and to ensure appropriate immune response. Here, the optimization of two methods to quantify S. flexneri 6 OAg is reported together with the characterization of their performances. The optimized Dische colorimetric method allows a tenfold increment of the sensitivity with respect to the original method and is useful for fast analysis detecting selectively methyl-pentoses, as rhamnose in S. flexneri 6 OAg. Also, a more specific HPAEC-PAD method was developed, detecting the dimer galacturonic acid-galactosamine (GalA-GalN) coming from S. flexneri 6 OAg acid hydrolysis. These methods will facilitate characterization of S. flexneri 6 OAg based vaccines. The colorimetric method can be used for quantification of other polysaccharide containing methyl-pentoses, and the HPAEC-PAD could be extended to other polysaccharides containing uronic acids.
polysaccharide, galacturonic acid, vaccine, quantification, HPAEC-PAD, design of experiment, Dische colorimetric method, methyl-pentose, S.flexneri O-antigen
Structure type: polymer chemical repeating unit ; 22000
Location inside paper: Fig. 1
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_133754,IEDB_136105,IEDB_137473,IEDB_225177,IEDB_885823
Methods: 13C NMR, 1H NMR, NMR-2D, ESI-MS, HPAEC-PAD, statistical analysis, HPLC-SEC, TFA/HCl hydrolysis, Dische colorimetric method
Related record ID(s): 9750
NCBI Taxonomy refs (TaxIDs): 424719
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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