A structure of the O-polysaccharide (O-antigen) of Escherichia coli O158 has been reported (Datta, A. K.; Basu, S.; Roy, N. Carbohydr. Res.1999, 322, 219-227). In this work, we reinvestigated the O158 polysaccharide using sugar analyses, Smith degradation, and (1)H and (13)C NMR spectroscopy and established the following structure, which is at variance with the structure established earlier: [see formula in text]. This structure is in agreement with the predicted functions of genes found in the O-antigen gene cluster of E. coli O158.
O-antigen, Escherichia coli, O-antigen gene cluster, O-Polysaccharide structure
NCBI PubMed ID: 21861990Publication DOI: 10.1016/j.carres.2011.07.021Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: perepel@ioc.ac.ru
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, GLC, mild acid hydrolysis, Smith degradation
Found