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1. (Article ID: 98)
Tambara Y, Hormaza JV, Perez C, Leon A, Arrieta J, Hernandez L
Structural analysis and optimised production of fructo-oligosaccharides by levansucrase from Acetobacter diazotrophicus SRT4
Biotechnology Letters 21(2) (1999)
117-121
Major fructo-oligosaccharides (FOS) produced by levansucrase (EC 2.4.1.10) from Acetobacter diazotrophicus SRT4 were characterised as 1-kestose and nystose by acid hydrolysis and 13C NMR spectroscopy. The highest yields of 1-kestose (481 mM; 241 g/l) and nystose (81 mM; 54 g/l) were achieved at initial sucrose concentration of 1754 mM (600 g/l), pH 5.5 and 40 C. The synthesized FOS reached 50% (w/w) of total sugars in the reaction mixture, with a conversion efficiency over 70% (w/w) based on the amount of sucrose converted to 1-kestose.
oligosaccharide, structural, analysis, Oligosaccharides, structural analysis, production, 1-kestose, Acetobacter, Acetobacter diazotrophicus, fructo-oligosaccharides, levansucrase, LsdA
Journal NLM ID: 8008051Publisher: Kluwer Academic Publishers
Correspondence: ipmlab
cigb.edu.cu
Institutions: Center for Genetic Engineering and Biotechnology, Ave 31 e/ 158 y 190, P.O. Box 6162, CP10600, Havana, Cuba, Cuban Institute of Sugar Research, Havana, Cuba, Havana University, Faculty of Chemistry, Havana, Cuba
Methods: 13C NMR
The publication contains the following compound(s):
- Compound ID: 347
Structure type: oligomer
Trivial name: 1-kestose, inulin
Compound class: fructan, fructo-oligosaccharide
Reference(s) to other database(s): GTC:G11260CN, GlycomeDB:
396, CCSD:
26122, CBank-STR:5624, GenDB:MH445969, US-PT:US2017298332A1
- Compound ID: 547
Structure type: oligomer
Trivial name: nystose, inulin, 1,1-kestotetraose
Compound class: fructan
Reference(s) to other database(s): GTC:G60726JL, GlycomeDB:
63, CCSD:
23519, CBank-STR:9595, GenDB:MH445969, US-PT:US2017298332A1
- Compound ID: 548
Structure type: oligomer
Trivial name: 6-kestose
Compound class: fructan
Reference(s) to other database(s): GTC:G48786WR, GlycomeDB:
6073
- Compound ID: 549
Structure type: homopolymer
Trivial name: levan, levan-type polysaccharide
Compound class: EPS, polysaccharide, fructan
Reference(s) to other database(s): GTC:G29465PK, GlycomeDB:
9593
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