The major antigenic determinants of the lipoglycans from Acholeplasma granularum and Acholeplasma axanthum were found to be the oligosaccharide sequences Glcp(β1→2)-Glcp(α1→4)-Glcp and Glcp(β1→2)-Glcp(β1→2)-Glcp, respectively. The disaccharides sophorose and maltose inhibited hemagglutination by specific antiserum of sheep erythrocytes coated with lipoglycan from A. granularum. Only sophorose possessed this capacity with the lipoglycan from A. axanthum. Periodate oxidation destroyed the ability of both lipoglycans to interact with specific antibody, a result compatible with structural data on the lipoglycans.
NCBI PubMed ID: 6188700Journal NLM ID: 0246127Publisher: American Society for Microbiology
Institutions: Department of Microbiology, University of South Dakota, Vermillion, South Dakota, USA
Methods: periodate oxidation, serological methods
Found