Show legend Show as text

Structure type: oligomer
Trivial name: GQ1b
Compound class: ganglioside
Contained glycoepitopes: IEDB_130648,IEDB_130675,IEDB_130676,IEDB_130678,IEDB_130679,IEDB_130680,IEDB_130681,IEDB_130683,IEDB_130684,IEDB_130686,IEDB_134627,IEDB_136044,IEDB_136095,IEDB_136794,IEDB_137339,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_147450,IEDB_147451,IEDB_149174,IEDB_150933,IEDB_150937,IEDB_153198,IEDB_153199,IEDB_190606,IEDB_433718,IEDB_547903,IEDB_858580,IEDB_983931,SB_116,SB_13,SB_14,SB_144,SB_164,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_2,SB_22,SB_23,SB_24,SB_25,SB_28,SB_3,SB_35,SB_37,SB_38,SB_39,SB_4,SB_41,SB_42,SB_5,SB_6,SB_68,SB_7,SB_70,SB_71,SB_76,SB_8,SB_84,SB_88,SB_94,SB_95,SB_96,SB_97,SB_98

• Article ID: 335
  Neisser A, Bernheimer H, Berger T, Moran AP, Schwerer B "Serum antibodies against gangliosides and Campylobacter jejuni lipopolysaccharides in Miller-Fisher syndrome" - Infection and Immunity 65(10) (1997) 4038-4042
  

  Seven patients with Miller Fisher syndrome (MFS), six in the acute phase and one in the recovery phase, were investigated for serum antibodies against gangliosides and purified lipopolysaccharides (LPS) from different strains of Campylobacter jejuni, including the MFS-associated serotypes O:2 and O:23. Immunoglobulin G antibodies against gangliosides GT1a and GQ1b were found in five of six patients in the acute phase of disease. Three of these patients also displayed antibodies to ganglioside GD2, a finding not previously reported for MFS. All anti-GT1a- and anti-GQ1b-seropositive patients showed antibody binding to C. jejuni LPS, predominantly to O:2 and O:23 LPS. Antibody cross-reactivity between gangliosides GT1a and GQ1b and O:2 and O:23 LPS was demonstrated by adsorption studies. This cross-reactivity between gangliosides and C.jejuni LPS, which is obviously due to oligosaccharide homologies, may be an important pathogenetic factor in the development of MFS after C. jejuni infection

  lipopolysaccharides, antibodies, Campylobacter, Campylobacter jejuni, gangliosides, Miller-Fisher syndrome

  NCBI PubMed ID: 9317004
  Journal NLM ID: 0246127
  Publisher: American Society for Microbiology
  Correspondence: andrea.neisser@univie.ac.at
  Institutions: Institute of Neurology and Department of Neurology, University of Vienna, Vienna, Austria, Department of Microbiology, University College, Galway, Ireland
  Methods: serological methods, TLC-immunostaining
  
   
  
  Campylobacter jejuni
  CSDB ID 7831 (all data & tools)

Show legend Show as text

Structure type: oligomer
Trivial name: GT1b
Compound class: ganglioside
Contained glycoepitopes: IEDB_130648,IEDB_130678,IEDB_130679,IEDB_130683,IEDB_130686,IEDB_134627,IEDB_136044,IEDB_136095,IEDB_136794,IEDB_137339,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_147450,IEDB_147451,IEDB_149174,IEDB_150933,IEDB_150937,IEDB_153198,IEDB_153199,IEDB_190606,IEDB_547903,IEDB_983931,SB_116,SB_13,SB_144,SB_164,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_2,SB_22,SB_23,SB_24,SB_25,SB_3,SB_35,SB_37,SB_39,SB_4,SB_41,SB_42,SB_5,SB_6,SB_68,SB_7,SB_70,SB_71,SB_76,SB_8,SB_84,SB_88,SB_96,SB_97,SB_98

• Article ID: 335
  Neisser A, Bernheimer H, Berger T, Moran AP, Schwerer B "Serum antibodies against gangliosides and Campylobacter jejuni lipopolysaccharides in Miller-Fisher syndrome" - Infection and Immunity 65(10) (1997) 4038-4042
  

  Seven patients with Miller Fisher syndrome (MFS), six in the acute phase and one in the recovery phase, were investigated for serum antibodies against gangliosides and purified lipopolysaccharides (LPS) from different strains of Campylobacter jejuni, including the MFS-associated serotypes O:2 and O:23. Immunoglobulin G antibodies against gangliosides GT1a and GQ1b were found in five of six patients in the acute phase of disease. Three of these patients also displayed antibodies to ganglioside GD2, a finding not previously reported for MFS. All anti-GT1a- and anti-GQ1b-seropositive patients showed antibody binding to C. jejuni LPS, predominantly to O:2 and O:23 LPS. Antibody cross-reactivity between gangliosides GT1a and GQ1b and O:2 and O:23 LPS was demonstrated by adsorption studies. This cross-reactivity between gangliosides and C.jejuni LPS, which is obviously due to oligosaccharide homologies, may be an important pathogenetic factor in the development of MFS after C. jejuni infection

  lipopolysaccharides, antibodies, Campylobacter, Campylobacter jejuni, gangliosides, Miller-Fisher syndrome

  NCBI PubMed ID: 9317004
  Journal NLM ID: 0246127
  Publisher: American Society for Microbiology
  Correspondence: andrea.neisser@univie.ac.at
  Institutions: Institute of Neurology and Department of Neurology, University of Vienna, Vienna, Austria, Department of Microbiology, University College, Galway, Ireland
  Methods: serological methods, TLC-immunostaining
  
   
  
  Campylobacter jejuni
  CSDB ID 7832 (all data & tools)

Show legend Show as text

Structure type: oligomer
Trivial name: GD2
Compound class: ganglioside
Contained glycoepitopes: IEDB_130648,IEDB_130676,IEDB_130679,IEDB_130683,IEDB_130684,IEDB_136044,IEDB_136095,IEDB_136794,IEDB_137339,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_150937,IEDB_153198,IEDB_153199,IEDB_190606,IEDB_433718,IEDB_858580,IEDB_983931,SB_116,SB_144,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_25,SB_3,SB_35,SB_37,SB_39,SB_4,SB_41,SB_42,SB_5,SB_6,SB_68,SB_7,SB_71,SB_76,SB_84,SB_88,SB_94,SB_95

• Article ID: 335
  Neisser A, Bernheimer H, Berger T, Moran AP, Schwerer B "Serum antibodies against gangliosides and Campylobacter jejuni lipopolysaccharides in Miller-Fisher syndrome" - Infection and Immunity 65(10) (1997) 4038-4042
  

  Seven patients with Miller Fisher syndrome (MFS), six in the acute phase and one in the recovery phase, were investigated for serum antibodies against gangliosides and purified lipopolysaccharides (LPS) from different strains of Campylobacter jejuni, including the MFS-associated serotypes O:2 and O:23. Immunoglobulin G antibodies against gangliosides GT1a and GQ1b were found in five of six patients in the acute phase of disease. Three of these patients also displayed antibodies to ganglioside GD2, a finding not previously reported for MFS. All anti-GT1a- and anti-GQ1b-seropositive patients showed antibody binding to C. jejuni LPS, predominantly to O:2 and O:23 LPS. Antibody cross-reactivity between gangliosides GT1a and GQ1b and O:2 and O:23 LPS was demonstrated by adsorption studies. This cross-reactivity between gangliosides and C.jejuni LPS, which is obviously due to oligosaccharide homologies, may be an important pathogenetic factor in the development of MFS after C. jejuni infection

  lipopolysaccharides, antibodies, Campylobacter, Campylobacter jejuni, gangliosides, Miller-Fisher syndrome

  NCBI PubMed ID: 9317004
  Journal NLM ID: 0246127
  Publisher: American Society for Microbiology
  Correspondence: andrea.neisser@univie.ac.at
  Institutions: Institute of Neurology and Department of Neurology, University of Vienna, Vienna, Austria, Department of Microbiology, University College, Galway, Ireland
  Methods: serological methods, TLC-immunostaining
  
   
  
  Campylobacter jejuni
  CSDB ID 7833 (all data & tools)

Show legend Show as text

Structure type: oligomer
Aglycon: Ceramide
Trivial name: GQ1b
Compound class: ganglioside
Contained glycoepitopes: IEDB_130648,IEDB_130675,IEDB_130676,IEDB_130678,IEDB_130679,IEDB_130680,IEDB_130681,IEDB_130683,IEDB_130684,IEDB_130686,IEDB_134627,IEDB_136044,IEDB_136794,IEDB_137339,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_147450,IEDB_147451,IEDB_149174,IEDB_150933,IEDB_150937,IEDB_153198,IEDB_153199,IEDB_190606,IEDB_433718,IEDB_547903,IEDB_858580,IEDB_983931,SB_116,SB_13,SB_14,SB_144,SB_164,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_2,SB_22,SB_23,SB_24,SB_25,SB_28,SB_3,SB_35,SB_37,SB_38,SB_39,SB_4,SB_41,SB_42,SB_5,SB_6,SB_68,SB_7,SB_70,SB_71,SB_76,SB_8,SB_84,SB_88,SB_94,SB_95,SB_96,SB_97,SB_98

• Article ID: 1689
  Jacobs BC, Endtz HP, van der Meche FGA, Hazenberg MP, Achtereekte HAM, Van Doorn PA "Serum anti-GQ1b IgG antibodies recognize surface epitopes on Campylobacter jejuni from patients with Miller Fisher syndrome" - Annals of Neurology 37 (1995) 260-264
  

  Three patients who had diarrhea prior to the development of Miller Fisher syndrome are presented. Campylobacter jejuni was isolated from stool specimens from all patients. High titers of anti-GQ1b IgG antibodies were demonstrated in the serum of these patients by enzyme-linked immunosorbent assay and thin-layer chromatography overlay. In enzyme-linked immunosorbent assay inhibition studies the anti-GQ1b IgG antibodies bound specifically to whole bacteria of the Miller Fisher syndrome-associated C. jejuni strains. The presence of anti-GQ1b IgG binding epitopes on the surface of the C. jejuni from the patients was not exclusively associated with a specific Penner serotype. It is suggested that anti-GQ1b antibodies are formed during the initial infection that elicits Miller Fisher syndrome. The cross-reactivity of anti-GQ1b IgG antibodies with surface epitopes on Miller Fisher syndrome-associated C. jejuni strains supports the hypothesis of molecular mimicry between bacteria and neural tissue.

  NCBI PubMed ID: 7531419
  Journal NLM ID: 7707449
  Institutions: Department of Neurology, University Hospital Dijkzigt, The Netherlands
  
   
  
  Campylobacter jejuni
  CSDB ID 103669 (all data & tools)

Show legend Show as text

Structure type: oligomer
Aglycon: Ceramide
Trivial name: GM3, II3NeuAc-Lac-Cer
Compound class: ganglioside
Contained glycoepitopes: IEDB_130679,IEDB_136044,IEDB_136794,IEDB_137339,IEDB_137472,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_190606,IEDB_983931,SB_116,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_3,SB_37,SB_39,SB_5,SB_6,SB_68,SB_7,SB_71,SB_76,SB_84,SB_88

• Article ID: 2536
  Ono E, Abe K, Nakazawa M, Naiki M "Ganglioside epitope recognized by K99 fimbriae from enterotoxigenic Escherichia coli" - Infection and Immunity 57 (1989) 907-911
  

  The receptor structure recognized by Escherichia coli possessing K99 fimbriae and by isolated K99 fimbrial fractions was examined by using an equine erythrocyte hemagglutination inhibition test. Both K99-positive organisms (strain B41) and fimbrial preparations reacted with N-glycolylneuraminyl-lactosyl-ceramide (NeuGcLacCer) purified from equine erythrocytes with very high potency. Fimbrial preparations were 253 times more potent than intact organisms, indicating that isolated fimbriae more precisely recognize the structure of NeuGcLacCer than do fimbriae located on the bacterial cell wall. Structurally, the N-glycolyl group of the sialic acid was shown to be essential because substitution of the N-acetyl group for the N-glycolyl group caused the reactivity to completely disappear. The substitution of the O-acetyl group for the C4 hydroxyl group of the sialic acid (4-O-Ac-NeuGcLacCer) also diminished the reactivity by about 500 times, indicating that the fine structure of NeuGc is necessary for recognition. N-Glycolylneuraminyl-neolactotetraosyl-ceramide (NeuGcnLc4Cer) and N-glycolylneuraminyl-neolactohexaosyl-ceramide (NeuGcnLc6Cer), both of which have identical disaccharides at the nonreducing terminal and longer carbohydrate chains, showed reduced reactivity, indicating that the ceramide of NeuGcLacCer is also involved in the recognition. Indeed, NeuGcLac oligosaccharides altered by cleavage of the ceramide or the terminal sialic acid (NeuGc) showed dramatically reduced reactivities. Ten other E. coli strains (isolated from diseased calves) and two strains (isolated from diseased piglets) which possessed the same K99 antigen and various O antigens were used for the recognition test. The results obtained were similar to those mentioned above.

  NCBI PubMed ID: 2465273
  Journal NLM ID: 0246127
  Publisher: American Society for Microbiology
  Institutions: Department of Biochemistry, Faculty of Veterinary Genetics, Hokkaido University, Sapporo, Japan
  
   
  
  Escherichia coli
  CSDB ID 130228 (all data & tools)

Show legend Show as text

Structure type: oligomer
Aglycon: Ceramide
Trivial name: GM1
Compound class: ganglioside
Contained glycoepitopes: IEDB_130648,IEDB_130678,IEDB_130679,IEDB_130683,IEDB_134627,IEDB_136044,IEDB_136794,IEDB_137339,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_147450,IEDB_147451,IEDB_149174,IEDB_150933,IEDB_190606,IEDB_547903,IEDB_983931,SB_116,SB_13,SB_144,SB_164,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_2,SB_23,SB_24,SB_25,SB_3,SB_37,SB_39,SB_4,SB_41,SB_5,SB_6,SB_68,SB_7,SB_71,SB_76,SB_8,SB_84,SB_88,SB_96

• Article ID: 1303
  Yuki N, Handa S, Tai T, Takahashi M, Saito K, Tsujino Y, Taki T "Ganglioside-like epitopes of lipopolysaccharides from Campylobacter jejuni (PEN 19) in three isolates from patients with Guillain-Barré syndrome" - Journal of the Neurological Sciences 130 (1995) 112-116
  

  Sera from patients with Guillain-Barre syndrome (GBS) frequently have anti-GM1 antibody. We earlier showed that an lipopolysaccharides (LPS) from Campylobacter jejuni (PEN 19) isolated from a GBS patient has a GM1 ganglioside-like structure. Aspinall et al. (Biochemistry,61 (1994) 335-337) reported that OH 4382 has an LPS that bears a CD3 ganglioside-like structure and that OH 4384 has an LPS that bears a GT1a-like structure; both strains were isolated from patients with GBS. They also suggested a GM1-like structure is present in the LPSs from OH 4384, but failed to show the presence in the LPSs from OH 4382. To clarify the pathogenesis of GBS after infection by C. jejuni (PEN 19), we investigated the carbohydrate structures of the three strains by thin-layer chromatography immunostaining with cholera toxin and monoclonal anti-ganglioside antibodies. We found that both OH 4382 and OH 4384 have an LPS with the GM1 epitope as well as one with the GT1a or GD3 epitope.

  Lipopolysaccharide, lipopolysaccharides, LPS, isolate, epitope, Campylobacter, Campylobacter jejuni, ganglioside, mimicry, syndrome, epitopes

  NCBI PubMed ID: 7544402
  Journal NLM ID: 0375403
  Publisher: Elsevier
  Institutions: Department of Biochemistry, Faculty of Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo113, Japan, Department of Tumor Immunology, Tokyo Metropolitan Institute of Medical Science, Bunkyo-ku, Tokyo, Japan, Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Shinjuku-ku, Tokyo, Japan, Department of Pediatrics, Ohtemae Hospital, Chuo-ku, Osaka, Japan
  Methods: NMR-2D, NMR
  
   
  
  Campylobacter jejuni PEN 19
  CSDB ID 141385 (all data & tools)
• Article ID: 2545
  Barbieri CL, Giorgio S, Merjan AJC, Figueiredo EN "Glycosphingolipid antigens of Leishmania (Leishmania) amazonensisamastigotes identified by use of a monoclonal antibody" - Infection and Immunity 61 (1993) 2131-2137
  
  Journal NLM ID: 0246127
  Publisher: American Society for Microbiology
  
   
  
  Leishmania amazonensis
  CSDB ID 130463 (all data & tools)

Show legend Show as text

Structure type: oligomer
Aglycon: Ceramide
Trivial name: GD1a
Compound class: ganglioside
Contained glycoepitopes: IEDB_130648,IEDB_130678,IEDB_130679,IEDB_130683,IEDB_134627,IEDB_136044,IEDB_136794,IEDB_137339,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_147450,IEDB_147451,IEDB_149174,IEDB_150933,IEDB_190606,IEDB_547903,IEDB_983931,SB_116,SB_13,SB_144,SB_164,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_2,SB_22,SB_23,SB_24,SB_25,SB_3,SB_37,SB_39,SB_4,SB_41,SB_5,SB_6,SB_68,SB_7,SB_70,SB_71,SB_76,SB_8,SB_84,SB_88,SB_96,SB_97,SB_98

• Article ID: 2545
  Barbieri CL, Giorgio S, Merjan AJC, Figueiredo EN "Glycosphingolipid antigens of Leishmania (Leishmania) amazonensisamastigotes identified by use of a monoclonal antibody" - Infection and Immunity 61 (1993) 2131-2137
  
  Journal NLM ID: 0246127
  Publisher: American Society for Microbiology
  
   
  
  Leishmania amazonensis
  CSDB ID 130464 (all data & tools)

Show legend Show as text

Structure type: oligomer
Aglycon: Ceramide
Trivial name: GD3
Compound class: ganglioside
Contained glycoepitopes: IEDB_130676,IEDB_130679,IEDB_136044,IEDB_136794,IEDB_137339,IEDB_137472,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_150937,IEDB_153198,IEDB_153199,IEDB_190606,IEDB_858580,IEDB_983931,SB_116,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_3,SB_35,SB_37,SB_39,SB_42,SB_5,SB_6,SB_68,SB_7,SB_71,SB_76,SB_84,SB_88,SB_95

• Article ID: 1303
  Yuki N, Handa S, Tai T, Takahashi M, Saito K, Tsujino Y, Taki T "Ganglioside-like epitopes of lipopolysaccharides from Campylobacter jejuni (PEN 19) in three isolates from patients with Guillain-Barré syndrome" - Journal of the Neurological Sciences 130 (1995) 112-116
  

  Sera from patients with Guillain-Barre syndrome (GBS) frequently have anti-GM1 antibody. We earlier showed that an lipopolysaccharides (LPS) from Campylobacter jejuni (PEN 19) isolated from a GBS patient has a GM1 ganglioside-like structure. Aspinall et al. (Biochemistry,61 (1994) 335-337) reported that OH 4382 has an LPS that bears a CD3 ganglioside-like structure and that OH 4384 has an LPS that bears a GT1a-like structure; both strains were isolated from patients with GBS. They also suggested a GM1-like structure is present in the LPSs from OH 4384, but failed to show the presence in the LPSs from OH 4382. To clarify the pathogenesis of GBS after infection by C. jejuni (PEN 19), we investigated the carbohydrate structures of the three strains by thin-layer chromatography immunostaining with cholera toxin and monoclonal anti-ganglioside antibodies. We found that both OH 4382 and OH 4384 have an LPS with the GM1 epitope as well as one with the GT1a or GD3 epitope.

  Lipopolysaccharide, lipopolysaccharides, LPS, isolate, epitope, Campylobacter, Campylobacter jejuni, ganglioside, mimicry, syndrome, epitopes

  NCBI PubMed ID: 7544402
  Journal NLM ID: 0375403
  Publisher: Elsevier
  Institutions: Department of Biochemistry, Faculty of Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo113, Japan, Department of Tumor Immunology, Tokyo Metropolitan Institute of Medical Science, Bunkyo-ku, Tokyo, Japan, Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Shinjuku-ku, Tokyo, Japan, Department of Pediatrics, Ohtemae Hospital, Chuo-ku, Osaka, Japan
  Methods: NMR-2D, NMR
  
   
  
  Campylobacter jejuni PEN 19
  CSDB ID 141386 (all data & tools)

Show legend Show as text

Structure type: oligomer
Aglycon: Ceramide
Trivial name: GT1a
Compound class: ganglioside
Contained glycoepitopes: IEDB_130648,IEDB_130678,IEDB_130679,IEDB_130683,IEDB_130686,IEDB_134627,IEDB_136044,IEDB_136794,IEDB_137339,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_147450,IEDB_147451,IEDB_149174,IEDB_150933,IEDB_150937,IEDB_153198,IEDB_153199,IEDB_190606,IEDB_547903,IEDB_983931,SB_116,SB_13,SB_144,SB_164,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_2,SB_22,SB_23,SB_24,SB_25,SB_3,SB_35,SB_37,SB_39,SB_4,SB_41,SB_42,SB_5,SB_6,SB_68,SB_7,SB_70,SB_71,SB_76,SB_8,SB_84,SB_88,SB_96,SB_97,SB_98

• Article ID: 1303
  Yuki N, Handa S, Tai T, Takahashi M, Saito K, Tsujino Y, Taki T "Ganglioside-like epitopes of lipopolysaccharides from Campylobacter jejuni (PEN 19) in three isolates from patients with Guillain-Barré syndrome" - Journal of the Neurological Sciences 130 (1995) 112-116
  

  Sera from patients with Guillain-Barre syndrome (GBS) frequently have anti-GM1 antibody. We earlier showed that an lipopolysaccharides (LPS) from Campylobacter jejuni (PEN 19) isolated from a GBS patient has a GM1 ganglioside-like structure. Aspinall et al. (Biochemistry,61 (1994) 335-337) reported that OH 4382 has an LPS that bears a CD3 ganglioside-like structure and that OH 4384 has an LPS that bears a GT1a-like structure; both strains were isolated from patients with GBS. They also suggested a GM1-like structure is present in the LPSs from OH 4384, but failed to show the presence in the LPSs from OH 4382. To clarify the pathogenesis of GBS after infection by C. jejuni (PEN 19), we investigated the carbohydrate structures of the three strains by thin-layer chromatography immunostaining with cholera toxin and monoclonal anti-ganglioside antibodies. We found that both OH 4382 and OH 4384 have an LPS with the GM1 epitope as well as one with the GT1a or GD3 epitope.

  Lipopolysaccharide, lipopolysaccharides, LPS, isolate, epitope, Campylobacter, Campylobacter jejuni, ganglioside, mimicry, syndrome, epitopes

  NCBI PubMed ID: 7544402
  Journal NLM ID: 0375403
  Publisher: Elsevier
  Institutions: Department of Biochemistry, Faculty of Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo113, Japan, Department of Tumor Immunology, Tokyo Metropolitan Institute of Medical Science, Bunkyo-ku, Tokyo, Japan, Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Shinjuku-ku, Tokyo, Japan, Department of Pediatrics, Ohtemae Hospital, Chuo-ku, Osaka, Japan
  Methods: NMR-2D, NMR
  
   
  
  Campylobacter jejuni PEN 19
  CSDB ID 141387 (all data & tools)

Show legend Show as text

Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130648,IEDB_130679,IEDB_130683,IEDB_136044,IEDB_136794,IEDB_137339,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_190606,IEDB_983931,SB_116,SB_144,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_25,SB_3,SB_37,SB_39,SB_4,SB_41,SB_5,SB_6,SB_68,SB_7,SB_71,SB_76,SB_84,SB_88

• Article ID: 3181
  Kanipes MI, Papp-Szabo E, Guerry P, Monteiro MA "Mutation of waaC, Encoding Heptosyltransferase I in Campylobacter jejuni 81-176, Affects the Structure of both Lipooligosaccharide and Capsular Carbohydrate" - Journal of Bacteriology 188(9) (2006) 3273-3279
  

  Campylobacter jejuni 81-176 lipooligosaccharide (LOS) is composed of two covalently linked domains: lipid A, a hydrophobic anchor, and a nonrepeating core oligosaccharide, consisting of an inner and outer core region. We report the isolation and characterization of the deepest rough C. jejuni 81-176 mutant by insertional mutagenesis into the waaC gene, encoding heptosyltransferase I that catalyzes the transfer of the first l-glycero-d-manno-heptose residue to 3-deoxy-d-manno-octulosonic residue (Kdo)-lipid A. Tricine gel electrophoresis, followed by silver staining, showed that site-specific mutation in the waaC gene resulted in the expression of a severely truncated LOS compared to wild-type strain 81-176. Gas-liquid chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy showed that the waaC LOS species lacked all sugars distal to Kdo-lipid A. Parallel structural studies of the capsular polysaccharides of the wild-type strain 81-176 and waaC mutant revealed loss of the 3-O-methyl group in the waaC mutant. Complementation of the C. jejuni mutant by insertion of the wild-type C. jejuni waaC gene into a chromosomal locus resulted in LOS and capsular structures identical to those expressed in the parent strain. We also report here the presence of O-methyl phosphoramidate in wild-type strain 81-176 capsular polysaccharide.

  capsular polysaccharide, Campylobacter jejuni, core region, heptosyltransferase I

  NCBI PubMed ID: 16621820
  Publication DOI: 10.1128/JB.188.9.3273-3279.2006
  Journal NLM ID: 2985120R
  Publisher: American Society for Microbiology
  Correspondence: guerryp@nmrc.navy.mil
  Institutions: Department of Chemistry, University of Guelph, Guelph, ON N1G 2W1, Canada, Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, and Enteric Diseases Department, Naval Medical Research Center, Silver Spring, Maryland 20910
  Methods: GLC-MS, FAB-MS, NMR, SDS-PAGE, GLC, composition analysis, genetic methods
  
   
  
  Campylobacter jejuni O23/O36 (81-176)
  CSDB ID 20528 (all data & tools)

Show legend Show as text

Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130679,IEDB_136044,IEDB_136794,IEDB_137339,IEDB_137472,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_190606,IEDB_983931,SB_116,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_3,SB_37,SB_39,SB_5,SB_6,SB_68,SB_7,SB_71,SB_76,SB_84,SB_88

• Article ID: 3181
  Kanipes MI, Papp-Szabo E, Guerry P, Monteiro MA "Mutation of waaC, Encoding Heptosyltransferase I in Campylobacter jejuni 81-176, Affects the Structure of both Lipooligosaccharide and Capsular Carbohydrate" - Journal of Bacteriology 188(9) (2006) 3273-3279
  

  Campylobacter jejuni 81-176 lipooligosaccharide (LOS) is composed of two covalently linked domains: lipid A, a hydrophobic anchor, and a nonrepeating core oligosaccharide, consisting of an inner and outer core region. We report the isolation and characterization of the deepest rough C. jejuni 81-176 mutant by insertional mutagenesis into the waaC gene, encoding heptosyltransferase I that catalyzes the transfer of the first l-glycero-d-manno-heptose residue to 3-deoxy-d-manno-octulosonic residue (Kdo)-lipid A. Tricine gel electrophoresis, followed by silver staining, showed that site-specific mutation in the waaC gene resulted in the expression of a severely truncated LOS compared to wild-type strain 81-176. Gas-liquid chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy showed that the waaC LOS species lacked all sugars distal to Kdo-lipid A. Parallel structural studies of the capsular polysaccharides of the wild-type strain 81-176 and waaC mutant revealed loss of the 3-O-methyl group in the waaC mutant. Complementation of the C. jejuni mutant by insertion of the wild-type C. jejuni waaC gene into a chromosomal locus resulted in LOS and capsular structures identical to those expressed in the parent strain. We also report here the presence of O-methyl phosphoramidate in wild-type strain 81-176 capsular polysaccharide.

  capsular polysaccharide, Campylobacter jejuni, core region, heptosyltransferase I

  NCBI PubMed ID: 16621820
  Publication DOI: 10.1128/JB.188.9.3273-3279.2006
  Journal NLM ID: 2985120R
  Publisher: American Society for Microbiology
  Correspondence: guerryp@nmrc.navy.mil
  Institutions: Department of Chemistry, University of Guelph, Guelph, ON N1G 2W1, Canada, Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, and Enteric Diseases Department, Naval Medical Research Center, Silver Spring, Maryland 20910
  Methods: GLC-MS, FAB-MS, NMR, SDS-PAGE, GLC, composition analysis, genetic methods
  
   
  
  Campylobacter jejuni O23/O36 (81-176)
  CSDB ID 20529 (all data & tools)

Show legend Show as text

Structure type: oligomer
Aglycon: ceramide
Trivial name: GM1
Compound class: ganglioside
Contained glycoepitopes: IEDB_130648,IEDB_130678,IEDB_130679,IEDB_130683,IEDB_134627,IEDB_136044,IEDB_136794,IEDB_137339,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_147450,IEDB_147451,IEDB_149174,IEDB_150933,IEDB_190606,IEDB_547903,IEDB_983931,SB_116,SB_13,SB_144,SB_164,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_2,SB_23,SB_24,SB_25,SB_3,SB_37,SB_39,SB_4,SB_41,SB_5,SB_6,SB_68,SB_7,SB_71,SB_76,SB_8,SB_84,SB_88,SB_96

• Article ID: 3646
  Komagamine T, Yuki N "Ganglioside mimicry as a cause of Guillain-Barre syndrome" - CNS and Neurological Disorders Drug Targets 5(4) (2006) 391-400
  

  Guillain-Barre syndrome (GBS), characterized by acute progressive limb weakness and areflexia, is the prototype of postinfectious autoimmune diseases. Campylobacter jejuni is the most frequently identified agent of infection in GBS patients, often preceding acute motor axonal neuropathy (AMAN), a variant of GBS. Anti-GM1, anti-GM1b, anti-GD1a, and anti-GalNAc-GD1a IgG antibodies are associated with AMAN. Carbohydrate mimicry [Galβ1-3GalNAcβ1-4(NeuAcα2-3)Galβ1-] was seen between the lipo-oligosaccharide of C. jejuni isolated from an AMAN patient and human GM1 ganglioside. Sensitization with the lipo-oligosaccharide of C. jejuni induces AMAN in rabbits as does sensitization with GM1 ganglioside. Paralyzed rabbits have pathological changes in their peripheral nerves identical to changes seen in human GBS. C. jejuni infection may induce anti-ganglioside antibodies by molecular mimicry, eliciting AMAN. This is the first verification of the causative mechanism of molecular mimicry in an autoimmune disease. To express ganglioside mimics, C. jejuni requires specific gene combinations that function in sialic acid biosynthesis or transfer. The knockout mutants of these landmark genes of GBS show reduced reactivity with GBS patients' sera, and fail to induce an anti-ganglioside antibody response in mice. These genes are crucial for the induction of neuropathogenic cross-reactive antibodies. An approach for evaluating intravenous immune globulin, a treatment for GBS, based on our animal model of AMAN is also discussed in this review, and recent advances made in this field are described.

  Campylobacter jejuni, ganglioside, Guillain-Barre syndrome, molecular mimicry, Autoimmune disease

  NCBI PubMed ID: 16918391
  Journal NLM ID: 101269155
  Publisher: Bentham Science Publishers
  Correspondence: yuki@dokkyomed.ac.jp
  Institutions: Department of Neurology and Research Institute for Neuroimmunological Diseases, Dokkyo Medical University School of Medicine, Tochigi, Japan
  
   
  
  Campylobacter jejuni
  CSDB ID 23623 (all data & tools)

Show legend Show as text

Structure type: structural motif or average structure
Trivial name: trisialoganglioside
Compound class: CPS
Contained glycoepitopes: IEDB_130648,IEDB_130676,IEDB_130678,IEDB_130679,IEDB_130680,IEDB_130681,IEDB_130683,IEDB_130684,IEDB_134627,IEDB_136044,IEDB_136794,IEDB_137339,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_147450,IEDB_147451,IEDB_149174,IEDB_150933,IEDB_150937,IEDB_153198,IEDB_153199,IEDB_190606,IEDB_433718,IEDB_547903,IEDB_858580,IEDB_983931,SB_116,SB_13,SB_14,SB_144,SB_164,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_2,SB_22,SB_23,SB_24,SB_25,SB_28,SB_3,SB_35,SB_37,SB_39,SB_4,SB_41,SB_42,SB_5,SB_6,SB_68,SB_7,SB_70,SB_71,SB_76,SB_8,SB_84,SB_88,SB_94,SB_95,SB_96,SB_97,SB_98

• Article ID: 4430
  Ovodov YS "Bacterial capsular antigens. Structural patterns of capsular antigens" - Biochemistry (Moscow) 71(9) (2006) 937-954
  

  Structural patterns of bacterial capsular antigens including capsular polysaccharides and exoglycans are given in this review. In addition, the immunological activity of capsular antigens and their role in type specificity of bacteria are discussed.

  structure, capsular polysaccharides, bacterial capsular antigens, bacterial exoglycans, immunological activity, type specificity

  NCBI PubMed ID: 17009947
  Publication DOI: 10.1134/S000629790609001X
  Journal NLM ID: 0376536
  Publisher: Nauka/Interperiodica
  Correspondence: ovoys@physiol.komisc.ru
  Institutions: Institute of Physiology, Komi Science Center, Urals Branch of the Russian Academy of Sciences, Syktyvkar 167982, Russia
  
   
  
  Escherichia coli K1
  CSDB ID 28748 (all data & tools)

Show legend Show as text

Structure type: oligomer
Trivial name: GM1
Compound class: ganglioside
Contained glycoepitopes: IEDB_130648,IEDB_130678,IEDB_130679,IEDB_130683,IEDB_134627,IEDB_136044,IEDB_136794,IEDB_137339,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_147450,IEDB_147451,IEDB_149174,IEDB_150933,IEDB_190606,IEDB_547903,IEDB_983931,SB_116,SB_13,SB_144,SB_164,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_2,SB_23,SB_24,SB_25,SB_3,SB_37,SB_39,SB_4,SB_41,SB_5,SB_6,SB_68,SB_7,SB_71,SB_76,SB_8,SB_84,SB_88,SB_96

• Article ID: 5525
  Yoshida F, Yoshinaka H, Tanaka H, Hanashima S, Yamaguchi Y, Ishihara M, Saburomaru M, Karo Y, Saito R, Ando H, Kiso M, Imamura A, Ishida H "Synthesis of the Core Oligosaccharides of Lipooligosaccharides from Campylobacter jejuni: A Putative Cause of Guillain-Barré Syndrome" - Chemistry: a European Journal 25(3) (2019) 796-805
  

  The chemical synthesis of the highly branched core oligosaccharides of lipooligosaccharides (LOSs) found in Campylobacter jejuni, which causes Guillain-Barré syndrome by a preceding infection, is described. The target LOS mimics, consisting of eight or nine monosaccharides, were classified into three groups as key building blocks: ganglioside-core tetra-/pentasaccharides (GM1-/GD1a-like), l-glycero-d-manno-heptose-containing trisaccharides, and 3-deoxy-d-manno-2-octulosonic acid (KDO) residues. These synthetic fragments were obtained from commercially available monosaccharides. Less obtainable l-glycero-d-manno-heptose and KDO residues, as key components of the LOSs, were synthesized from p-methoxyphenyl d-mannoside and di-O-isopropylidene-protected d-mannose, respectively. The synthesis of α-KDO glycoside, as one of the most difficult stereocontrolled glycosidic constructions, was achieved by treating a 2,3-ene derivative of KDO with phenylselenyl trifluoromethanesulfonate as a suitable alpha-directing reagent. All synthetic blocks were constructed through a convergent synthetic route, which resulted in the first synthesis of structurally challenging LOS core glycans containing ganglioside GM1 and GD1a-core sequences.

  chemistry, structural, group, Research, gangliosides, Guillain-Barre syndrome, cluster, lipooligosaccharides, glycosylation, PDF, Science, glycobiology, arbohydrates

  NCBI PubMed ID: 30351481
  Publication DOI: 10.1002/chem.201804862
  Journal NLM ID: 9513783
  Publisher: Weinheim: VCH Verlagsgesellschaft/Verlag I
  Correspondence: Akihiro Imamura ; Hideharu Ishida
  Institutions: Department of Applied Bio-organic Chemistry, Gifu University, 1-1 Yanagido, Gifu-shi, Gifu, 501-1193, Japan, Center for Highly Advanced Integration and Nano and Life Sciences, (G-CHAIN), Gifu University, 1-1 Yanagido, Gifu-shi, Gifu, 501-1193, Japan, Structural Glycobiology Team, Systems Glycobiology Research Group, RIKEN Global Research Cluster, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan
  Methods: 13C NMR, 1H NMR, TLC, chemical synthesis, UV, glycosylation, optical rotation measurement, ESI-TOF-MS, flash chromatography
  
   
  
  Campylobacter jejuni PEN 19
  CSDB ID 2360 (all data & tools)