Show legend Show as text

Structure type: oligomer
Trivial name: GQ1b
Compound class: ganglioside
Contained glycoepitopes: IEDB_130648,IEDB_130675,IEDB_130676,IEDB_130678,IEDB_130679,IEDB_130680,IEDB_130681,IEDB_130683,IEDB_130684,IEDB_130686,IEDB_134627,IEDB_136044,IEDB_136095,IEDB_136794,IEDB_137339,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_147450,IEDB_147451,IEDB_149174,IEDB_150933,IEDB_150937,IEDB_153198,IEDB_153199,IEDB_190606,IEDB_433718,IEDB_547903,IEDB_858580,IEDB_983931,SB_116,SB_13,SB_14,SB_144,SB_164,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_2,SB_22,SB_23,SB_24,SB_25,SB_28,SB_3,SB_35,SB_37,SB_38,SB_39,SB_4,SB_41,SB_42,SB_5,SB_6,SB_68,SB_7,SB_70,SB_71,SB_76,SB_8,SB_84,SB_88,SB_94,SB_95,SB_96,SB_97,SB_98

• Article ID: 335
  Neisser A, Bernheimer H, Berger T, Moran AP, Schwerer B "Serum antibodies against gangliosides and Campylobacter jejuni lipopolysaccharides in Miller-Fisher syndrome" - Infection and Immunity 65(10) (1997) 4038-4042
  

  Seven patients with Miller Fisher syndrome (MFS), six in the acute phase and one in the recovery phase, were investigated for serum antibodies against gangliosides and purified lipopolysaccharides (LPS) from different strains of Campylobacter jejuni, including the MFS-associated serotypes O:2 and O:23. Immunoglobulin G antibodies against gangliosides GT1a and GQ1b were found in five of six patients in the acute phase of disease. Three of these patients also displayed antibodies to ganglioside GD2, a finding not previously reported for MFS. All anti-GT1a- and anti-GQ1b-seropositive patients showed antibody binding to C. jejuni LPS, predominantly to O:2 and O:23 LPS. Antibody cross-reactivity between gangliosides GT1a and GQ1b and O:2 and O:23 LPS was demonstrated by adsorption studies. This cross-reactivity between gangliosides and C.jejuni LPS, which is obviously due to oligosaccharide homologies, may be an important pathogenetic factor in the development of MFS after C. jejuni infection

  lipopolysaccharides, antibodies, Campylobacter, Campylobacter jejuni, gangliosides, Miller-Fisher syndrome

  NCBI PubMed ID: 9317004
  Journal NLM ID: 0246127
  Publisher: American Society for Microbiology
  Correspondence: andrea.neisser@univie.ac.at
  Institutions: Institute of Neurology and Department of Neurology, University of Vienna, Vienna, Austria, Department of Microbiology, University College, Galway, Ireland
  Methods: serological methods, TLC-immunostaining
  
   
  
  Campylobacter jejuni
  CSDB ID 7831 (all data & tools)

Show legend Show as text

Structure type: oligomer
Trivial name: GD2
Compound class: ganglioside
Contained glycoepitopes: IEDB_130648,IEDB_130676,IEDB_130679,IEDB_130683,IEDB_130684,IEDB_136044,IEDB_136095,IEDB_136794,IEDB_137339,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_150937,IEDB_153198,IEDB_153199,IEDB_190606,IEDB_433718,IEDB_858580,IEDB_983931,SB_116,SB_144,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_25,SB_3,SB_35,SB_37,SB_39,SB_4,SB_41,SB_42,SB_5,SB_6,SB_68,SB_7,SB_71,SB_76,SB_84,SB_88,SB_94,SB_95

• Article ID: 335
  Neisser A, Bernheimer H, Berger T, Moran AP, Schwerer B "Serum antibodies against gangliosides and Campylobacter jejuni lipopolysaccharides in Miller-Fisher syndrome" - Infection and Immunity 65(10) (1997) 4038-4042
  

  Seven patients with Miller Fisher syndrome (MFS), six in the acute phase and one in the recovery phase, were investigated for serum antibodies against gangliosides and purified lipopolysaccharides (LPS) from different strains of Campylobacter jejuni, including the MFS-associated serotypes O:2 and O:23. Immunoglobulin G antibodies against gangliosides GT1a and GQ1b were found in five of six patients in the acute phase of disease. Three of these patients also displayed antibodies to ganglioside GD2, a finding not previously reported for MFS. All anti-GT1a- and anti-GQ1b-seropositive patients showed antibody binding to C. jejuni LPS, predominantly to O:2 and O:23 LPS. Antibody cross-reactivity between gangliosides GT1a and GQ1b and O:2 and O:23 LPS was demonstrated by adsorption studies. This cross-reactivity between gangliosides and C.jejuni LPS, which is obviously due to oligosaccharide homologies, may be an important pathogenetic factor in the development of MFS after C. jejuni infection

  lipopolysaccharides, antibodies, Campylobacter, Campylobacter jejuni, gangliosides, Miller-Fisher syndrome

  NCBI PubMed ID: 9317004
  Journal NLM ID: 0246127
  Publisher: American Society for Microbiology
  Correspondence: andrea.neisser@univie.ac.at
  Institutions: Institute of Neurology and Department of Neurology, University of Vienna, Vienna, Austria, Department of Microbiology, University College, Galway, Ireland
  Methods: serological methods, TLC-immunostaining
  
   
  
  Campylobacter jejuni
  CSDB ID 7833 (all data & tools)

Show legend Show as text

Structure type: oligomer
Aglycon: Ceramide
Trivial name: GQ1b
Compound class: ganglioside
Contained glycoepitopes: IEDB_130648,IEDB_130675,IEDB_130676,IEDB_130678,IEDB_130679,IEDB_130680,IEDB_130681,IEDB_130683,IEDB_130684,IEDB_130686,IEDB_134627,IEDB_136044,IEDB_136794,IEDB_137339,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_147450,IEDB_147451,IEDB_149174,IEDB_150933,IEDB_150937,IEDB_153198,IEDB_153199,IEDB_190606,IEDB_433718,IEDB_547903,IEDB_858580,IEDB_983931,SB_116,SB_13,SB_14,SB_144,SB_164,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_2,SB_22,SB_23,SB_24,SB_25,SB_28,SB_3,SB_35,SB_37,SB_38,SB_39,SB_4,SB_41,SB_42,SB_5,SB_6,SB_68,SB_7,SB_70,SB_71,SB_76,SB_8,SB_84,SB_88,SB_94,SB_95,SB_96,SB_97,SB_98

• Article ID: 1689
  Jacobs BC, Endtz HP, van der Meche FGA, Hazenberg MP, Achtereekte HAM, Van Doorn PA "Serum anti-GQ1b IgG antibodies recognize surface epitopes on Campylobacter jejuni from patients with Miller Fisher syndrome" - Annals of Neurology 37 (1995) 260-264
  

  Three patients who had diarrhea prior to the development of Miller Fisher syndrome are presented. Campylobacter jejuni was isolated from stool specimens from all patients. High titers of anti-GQ1b IgG antibodies were demonstrated in the serum of these patients by enzyme-linked immunosorbent assay and thin-layer chromatography overlay. In enzyme-linked immunosorbent assay inhibition studies the anti-GQ1b IgG antibodies bound specifically to whole bacteria of the Miller Fisher syndrome-associated C. jejuni strains. The presence of anti-GQ1b IgG binding epitopes on the surface of the C. jejuni from the patients was not exclusively associated with a specific Penner serotype. It is suggested that anti-GQ1b antibodies are formed during the initial infection that elicits Miller Fisher syndrome. The cross-reactivity of anti-GQ1b IgG antibodies with surface epitopes on Miller Fisher syndrome-associated C. jejuni strains supports the hypothesis of molecular mimicry between bacteria and neural tissue.

  NCBI PubMed ID: 7531419
  Journal NLM ID: 7707449
  Institutions: Department of Neurology, University Hospital Dijkzigt, The Netherlands
  
   
  
  Campylobacter jejuni
  CSDB ID 103669 (all data & tools)

Show legend Show as text

Structure type: oligomer
Aglycon: Ceramide
Trivial name: GD3
Compound class: ganglioside
Contained glycoepitopes: IEDB_130676,IEDB_130679,IEDB_136044,IEDB_136794,IEDB_137339,IEDB_137472,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_150937,IEDB_153198,IEDB_153199,IEDB_190606,IEDB_858580,IEDB_983931,SB_116,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_3,SB_35,SB_37,SB_39,SB_42,SB_5,SB_6,SB_68,SB_7,SB_71,SB_76,SB_84,SB_88,SB_95

• Article ID: 1303
  Yuki N, Handa S, Tai T, Takahashi M, Saito K, Tsujino Y, Taki T "Ganglioside-like epitopes of lipopolysaccharides from Campylobacter jejuni (PEN 19) in three isolates from patients with Guillain-Barré syndrome" - Journal of the Neurological Sciences 130 (1995) 112-116
  

  Sera from patients with Guillain-Barre syndrome (GBS) frequently have anti-GM1 antibody. We earlier showed that an lipopolysaccharides (LPS) from Campylobacter jejuni (PEN 19) isolated from a GBS patient has a GM1 ganglioside-like structure. Aspinall et al. (Biochemistry,61 (1994) 335-337) reported that OH 4382 has an LPS that bears a CD3 ganglioside-like structure and that OH 4384 has an LPS that bears a GT1a-like structure; both strains were isolated from patients with GBS. They also suggested a GM1-like structure is present in the LPSs from OH 4384, but failed to show the presence in the LPSs from OH 4382. To clarify the pathogenesis of GBS after infection by C. jejuni (PEN 19), we investigated the carbohydrate structures of the three strains by thin-layer chromatography immunostaining with cholera toxin and monoclonal anti-ganglioside antibodies. We found that both OH 4382 and OH 4384 have an LPS with the GM1 epitope as well as one with the GT1a or GD3 epitope.

  Lipopolysaccharide, lipopolysaccharides, LPS, isolate, epitope, Campylobacter, Campylobacter jejuni, ganglioside, mimicry, syndrome, epitopes

  NCBI PubMed ID: 7544402
  Journal NLM ID: 0375403
  Publisher: Elsevier
  Institutions: Department of Biochemistry, Faculty of Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo113, Japan, Department of Tumor Immunology, Tokyo Metropolitan Institute of Medical Science, Bunkyo-ku, Tokyo, Japan, Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Shinjuku-ku, Tokyo, Japan, Department of Pediatrics, Ohtemae Hospital, Chuo-ku, Osaka, Japan
  Methods: NMR-2D, NMR
  
   
  
  Campylobacter jejuni PEN 19
  CSDB ID 141386 (all data & tools)

Show legend Show as text

Structure type: oligomer
Trivial name: tetrasaccharide GD3-like epitope
Contained glycoepitopes: IEDB_130676,IEDB_130679,IEDB_136044,IEDB_136794,IEDB_137472,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_144998,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_150937,IEDB_153198,IEDB_153199,IEDB_190606,IEDB_858580,IEDB_983931,SB_116,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_35,SB_37,SB_39,SB_42,SB_6,SB_68,SB_7,SB_76,SB_84,SB_88

• Article ID: 3184
  Usuki S, Thompson SA, Rivner MH, Taguchi K, Shibata K, Ariga T, Yu RK "Molecular mimicry: Sensitization of Lewis rats with Campylobacter jejuni lipopolysaccharides induces formation of antibody toward GD3 ganglioside" - Journal of Neuroscience Research 83(2) (2006) 274-284
  

  Recently we have reported cases of demyelinating inflammatory neuropathy showing elevated titers of anti-GD3 antibodies, which occurs rarely in Guillain-Barre syndrome. To examine the correlation between the anti-GD3 antibody titer and Campylobacter jejuni infection, we sensitized female Lewis rats with lipopolysaccharides (LPSs) from serotype HS19 of C. jejuni and examined changes in nerve conduction velocity and nerve conduction block (P/D ratio). After 16 weeks of sensitization, animals revealed decreases of nerve conduction velocity and conduction block (P/D ratio) and high titer of anti-GD3 antibodies. These anti-GD3 antibodies also blocked transmission in neuromuscular junctions of spinal cord-muscle cells cocultures. The GD3 epitope was verified to be located on the Schwann cell surface and nodes of Ranvier in rat sciatic nerve. To determine the target epitope for GD3 antibodies in causing nerve dysfunction, the LPS fraction containing the GD3 epitope was purified from the total LPS by using an anti-GD3 monoclonal antibody-immobilized affinity column. Subsequently, chemical analysis of the oligosaccharide portion was performed and confirmed the presence of a GD3-like epitope as having the following tetrasaccharide structure: NeuAcα2-8NeuAc2-3Galβ1-4Hep. Our data thus support the possibility of a contribution of GD3 mimicry as a potential pathogenic mechanism of peripheral nerve dysfunction.

  LPS, antibody, Campylobacter, Campylobacter jejuni, ganglioside, Guillain-Barre syndrome, anti-gangliosid antibody, GD3

  NCBI PubMed ID: 16342208
  Publication DOI: 10.1002/jnr.20717
  Journal NLM ID: 7600111
  Publisher: New York, NY: Wiley Interscience
  Correspondence: ryu@mcg.edu
  Institutions: Institute of Molecular Medicine and Genetics and Institute of Neuroscience, Medical College of Georgia, Augusta, Georgia, Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, Georgia, Department of Neurology, Medical College of Georgia, Augusta, Georgia, Department of Neuroscience, Showa Pharmaceutical University, Tokyo, Japan
  Methods: chemical analysis, MALDI-TOF MS, composition analysis, serological methods
  
   
  
  Campylobacter jejuni ATCC 43446 HS19
  CSDB ID 20201 (all data & tools)

Show legend Show as text

Structure type: fragment of a bigger structure
Aglycon: (1->4) core Hep I
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130676,IEDB_130679,IEDB_136044,IEDB_136794,IEDB_137472,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_150937,IEDB_153198,IEDB_153199,IEDB_190606,IEDB_858580,IEDB_983931,SB_116,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_35,SB_37,SB_39,SB_42,SB_6,SB_68,SB_7,SB_76,SB_84,SB_88

• Article ID: 3345
  Schweda EK, Richards JC, Hood DW, Moxon ER "Expression and structural diversity of the lipopolysaccharide of Haemophilus influenzae: implication in virulence" - International Journal of Medical Microbiology 297(5) (2007) 297-306
  

  Lipopolysaccharide (LPS) is a major virulence determinant of the human bacterial pathogen Haemophilus influenzae. A characteristic feature of H. influenzae LPS is the extensive intra- and inter-strain heterogeneity of glycoform structure which is key to the role of the molecule in both commensal and disease-causing behaviour of the bacterium. Through the combination of genetics and detailed structural analyses, H. influenzae is an exemplar Gram-negative bacterium for which now the most extensive and detailed LPS structural data and functional correlates are available. LPS from H. influenzae consists of a conserved glucose-substituted triheptosyl inner-core moiety L-α-D-Hepp-(1→2)-[PEtn→6]-L-α-D-Hepp-(1→3)-[β-D-Glcp-(1→4)]-L-α-D-Hepp linked to lipid A via Kdo 4-phosphate. The inner-core unit provides the template for attachment of oligosaccharide- and non-carbohydrate substituents. Here, the structure, genetics and expression of LPS glycoforms in the outer core are reviewed as well as their implication on virulence

  Lipopolysaccharide, Haemophilus influenzae, molecular mimicry, Phosphocholine, sialic acid, Digalactoside

  NCBI PubMed ID: 17452015
  Publication DOI: 10.1016/j.ijmm.2007.03.007
  Journal NLM ID: 100898849
  Publisher: Jena, Germany: Urban & Fischer Verlag
  Correspondence: elke.schweda@crc.ki.se
  Institutions: Clinical Research Centre, Karolinska Institutet and University College of South Stockholm, NOVUM, S-141 86 Huddinge, Sweden
  
   
  
  Haemophilus influenzae
  CSDB ID 21831 (all data & tools)

Show legend Show as text

Structure type: fragment of a bigger structure
Aglycon: (1->2) core Hep III
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130676,IEDB_130679,IEDB_136044,IEDB_136794,IEDB_137472,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_150937,IEDB_153198,IEDB_153199,IEDB_190606,IEDB_858580,IEDB_983931,SB_116,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_35,SB_37,SB_39,SB_42,SB_6,SB_68,SB_7,SB_76,SB_84,SB_88

• Article ID: 3345
  Schweda EK, Richards JC, Hood DW, Moxon ER "Expression and structural diversity of the lipopolysaccharide of Haemophilus influenzae: implication in virulence" - International Journal of Medical Microbiology 297(5) (2007) 297-306
  

  Lipopolysaccharide (LPS) is a major virulence determinant of the human bacterial pathogen Haemophilus influenzae. A characteristic feature of H. influenzae LPS is the extensive intra- and inter-strain heterogeneity of glycoform structure which is key to the role of the molecule in both commensal and disease-causing behaviour of the bacterium. Through the combination of genetics and detailed structural analyses, H. influenzae is an exemplar Gram-negative bacterium for which now the most extensive and detailed LPS structural data and functional correlates are available. LPS from H. influenzae consists of a conserved glucose-substituted triheptosyl inner-core moiety L-α-D-Hepp-(1→2)-[PEtn→6]-L-α-D-Hepp-(1→3)-[β-D-Glcp-(1→4)]-L-α-D-Hepp linked to lipid A via Kdo 4-phosphate. The inner-core unit provides the template for attachment of oligosaccharide- and non-carbohydrate substituents. Here, the structure, genetics and expression of LPS glycoforms in the outer core are reviewed as well as their implication on virulence

  Lipopolysaccharide, Haemophilus influenzae, molecular mimicry, Phosphocholine, sialic acid, Digalactoside

  NCBI PubMed ID: 17452015
  Publication DOI: 10.1016/j.ijmm.2007.03.007
  Journal NLM ID: 100898849
  Publisher: Jena, Germany: Urban & Fischer Verlag
  Correspondence: elke.schweda@crc.ki.se
  Institutions: Clinical Research Centre, Karolinska Institutet and University College of South Stockholm, NOVUM, S-141 86 Huddinge, Sweden
  
   
  
  Haemophilus influenzae
  CSDB ID 21836 (all data & tools)

Show legend Show as text

Structure type: oligomer
Trivial name: oligosaccharide GD1b and GD2 mimics
Contained glycoepitopes: IEDB_120354,IEDB_123890,IEDB_130648,IEDB_130650,IEDB_130676,IEDB_130678,IEDB_130679,IEDB_130681,IEDB_130683,IEDB_130684,IEDB_134627,IEDB_136044,IEDB_136794,IEDB_137472,IEDB_137473,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_147450,IEDB_147451,IEDB_149174,IEDB_150933,IEDB_150937,IEDB_153198,IEDB_153199,IEDB_190606,IEDB_2189047,IEDB_433718,IEDB_547903,IEDB_858580,IEDB_983931,SB_116,SB_144,SB_164,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_23,SB_24,SB_25,SB_35,SB_37,SB_39,SB_42,SB_6,SB_68,SB_7,SB_76,SB_8,SB_84,SB_88,SB_96

• Article ID: 3903
  Moran A "The Role of Endotoxin in Infection: Helicobacter pylori and Campylobacter jejuni" - Book: Endotoxins: Structure, Function and Recognition (series: Subcellular Biochemistry, Part 1) (2010) Vol. 53, Chapter 10, 209-240
  

  Both Helicobacter pylori and Campylobacter jejuni are highly prevalent Gram-negative microaerophilic bacteria which are gastrointestinal pathogens of humans; H. pylori colonizes the gastroduodenal compartment and C. jejuni the intestinal mucosa. Although H. pylori causes chronic gastric infection leading to gastritis, peptic ulcers and eventually gastric cancer while C. jejuni causes acute infection inducing diarrhoeal disease, the endotoxin molecules of both bacterial species contrastingly contribute to their pathogenesis and the autoimmune sequelae each induces. Compared with enterobacterial endotoxin, that of H. pylori has significantly lower endotoxic and immuno-activities, the molecular basis for which is the underphosphorylation and underacylation of the lipid A component that interacts with immune receptors. This induction of low immunological responsiveness by endotoxin may aid the prolongation of H. pylori infection and therefore infection chronicity. On the other hand, this contrasts with acute infection-causing C. jejuni where overt inflammation contributes to pathology and diarrhoea production, and whose endotoxin is immunologically and endotoxically active. Futhermore, both H. pylori and C. jejuni exhibit molecular mimicry in the saccharide components of their endotoxins which can induce autoreactive antibodies; H. pylori expresses mimicry of Lewis and some ABO blood group antigens, C. jejuni mimicry of gangliosides. The former has been implicated in influencing the development of inflammation and gastric atrophy (a precursor of gastic cancer), the latter is central to the development of the neurological disorder Guillain-Barre syndrome. Both diseases raise important questions concerning infection-induced autoimmunity awaiting to be addressed.

  lipid A, Campylobacter jejuni, molecular mimicry, Helicobacter pylori, bacterial pathogenesis

  NCBI PubMed ID: 20593269
  Publication DOI: 10.1007/978-90-481-9078-2_10
  Publisher: Springer Science+Business Media B.V.
  Correspondence: anthony.moran@nuigalway.ie
  Editors: Wang X, Quinn PJ
  Institutions: Laboratory of Molecular Biochemistry, Microbiology, School of Natural Sciences, National University of Ireland, Galway, Ireland
  
   
  
  Campylobacter jejuni 81-176
  CSDB ID 25779 (all data & tools)

Show legend Show as text

Structure type: oligomer
Trivial name: disialylated glycoform
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_115009,IEDB_116046,IEDB_120354,IEDB_123890,IEDB_130648,IEDB_130651,IEDB_130676,IEDB_130679,IEDB_136044,IEDB_136794,IEDB_136906,IEDB_137338,IEDB_137472,IEDB_137473,IEDB_137779,IEDB_1391964,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_140624,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_150937,IEDB_151528,IEDB_152217,IEDB_153198,IEDB_153199,IEDB_190606,IEDB_2189047,IEDB_241118,IEDB_423106,IEDB_742247,IEDB_858580,IEDB_983931,SB_116,SB_165,SB_166,SB_167,SB_170,SB_171,SB_172,SB_178,SB_187,SB_192,SB_195,SB_21,SB_31,SB_35,SB_37,SB_39,SB_42,SB_6,SB_61,SB_62,SB_68,SB_7,SB_76,SB_84,SB_88

• Article ID: 4125
  Twelkmeyer B, Burstrom PK, Li J, Richard ME, Hood DW, Schweda EK "Expression of a new disialyllacto structure in the lipopolysaccharide of non-typeable Haemophilus influenzae" - Carbohydrate Research 346(13) (2011) 1885-1897
  

  The structure of lipopolysaccharide (LPS) expressed by non-typeable Haemophilus influenzae (NTHi) strains 1008 and 1247 has been investigated by mass spectrometry and NMR analyses on O-deacylated LPS and core oligosaccharide material. Both strains express the conserved triheptosyl inner core, [L-α-D-Hepp-(1→2)-[PEtn→6]-L-α-D-Hepp-(1→3)-L-α-D-Hepp-(1→5)-[PPEtn→4]-α-Kdo-(2→6)-Lipid A] with PCho→6)-β-D-Glcp (GlcI) substituting the proximal heptose (HepI) at O-4. Strain 1247 expresses the common structural motifs of H. influenzae; globotetraose [β-D-GalpNAc-(1→3)-α-D-Galp-(1→4)-β-D-Galp-(1→4)-β-D-Glcp-(1→] and its truncated versions globoside [α-D-Galp-(1→4)-β-D-Galp-(1→4)-β-D-Glcp-(1→] and lactose [β-D-Galp-(1→4)-β-D-Glcp-(1→] linked to the terminal heptose of the inner core and GlcI. A genetically distinct NTHi strain, 1008, expresses identical structures to strain 1247 with the exception that it lacks GalNAc. A lpsA mutant of strain 1247 expressed LPS of reduced complexity that facilitated unambiguous structural determination of the oligosaccharide from HepI. By CE-ESI-MS/MS we identified disialylated glycoforms indicating disialyllactose [α-Neu5Ac-(2→8)-α-Neu5Ac-(2→3)-β-D-Gal-(1→4)-β-D-Glcp-(1→] as an extension from GlcI which is a novel finding for NTHi LPS.

  Lipopolysaccharide, Haemophilus influenzae, otitis media, Digalactoside, disialyllactoside

  NCBI PubMed ID: 21683945
  Publication DOI: 10.1016/j.carres.2011.05.020
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: E.K.H. Schweda
  Institutions: Karolinska Institutet, Clinical Research Centre, Huddinge, Sweden
  Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, HF solvolysis, ESI-MS, mild acid hydrolysis, de-O-acylation with hydrazine, composition analysis, NMR-1D, alkaline deacylation, HPAEC-PAD, CE-ESI-MS/MS, CE-ESI-MS, LC-ESI-MS
  
   
  
  Haemophilus influenzae NTHi 1247lpsA
  CSDB ID 26373 (all data & tools)

Show legend Show as text

Structure type: oligomer
Aglycon: (CH2)2NH
Trivial name: GD3
Compound class: CPS
Contained glycoepitopes: IEDB_130676,IEDB_130679,IEDB_136044,IEDB_136794,IEDB_137472,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_150937,IEDB_153198,IEDB_153199,IEDB_190606,IEDB_858580,IEDB_983931,SB_116,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_35,SB_37,SB_39,SB_42,SB_6,SB_68,SB_7,SB_76,SB_84,SB_88

• Article ID: 4373
  Pincus SH, Moran E, Maresh G, Jennings HJ, Pritchard DG, Egan ML, Blixt O "Fine specificity and cross-reactions of monoclonal antibodies to group B streptococcal capsular polysaccharide type III" - Vaccine 30(32) (2012) 4849-4858
  

  Group B streptococcus (GBS) is a major cause of neonatal sepsis and meningitis. Despite aggressive campaigns using antenatal prophylactic antibiotic therapy, infections continue. Developing an effective maternal vaccine is a public health priority. Antibody (Ab) to the capsular polysaccharide (CPS) is considered the dominant 'protective' immune mediator. Here we study the fine specificity and potential host reactivity of a panel of well-characterized murine monoclonal Abs against the type III CPS by examining the binding of the Abs to intact and neuraminidase-digested GBS, purified CPS, synthetic carbohydrate structures, and cells. The results showed marked differences in the fine specificity among these mAbs to a single carbohydrate structure. Cross-reactions with synthetic GD3 and GT3 carbohydrates, representing structures found on surfaces of neural and developing cells, were demonstrated using carbohydrate array technology. The anti-CPS(III) mAbs did not react with cells expressing GD3 and GT3, nor did mAbs specific for the host carbohydrates cross-react with GBS, raising questions about the physiological relevance of this cross-reaction. But in the process of these investigations, we serendipitously demonstrated cross-reactions of some anti-CPS(III) mAbs with antigens, likely carbohydrates, found on human leukocytes. These studies suggest caution in the development of a maternal vaccine to prevent infection by this important human pathogen.

  capsular polysaccharide, group B Streptococcus, cross-reaction, monoclonal Ab, fine specificity, carbohydrate array

  NCBI PubMed ID: 22634296
  Publication DOI: 10.1016/j.vaccine.2012.05.006
  Journal NLM ID: 8406899
  Publisher: Elsevier
  Correspondence: spincus@chnola-research.org
  Institutions: Research Institute for Children, Children's Hospital, New Orleans, LA 70118, United States, Institute for Biological Sciences, National Research Council of Canada, Ottawa, ON, Canada K1A 0R6, Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL 35294, United States, Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, United States, Copenhagen Center for Glycomics (CCG), Departments of Cellular and Molecular Medicine and Dentistry, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, DK-2200, Copenhagen N, Denmark
  Methods: ELISA, biological assays, serological methods, binding assays, immunofluorescence analyses
  
   
  
  Streptococcus sp. B
  CSDB ID 28583 (all data & tools)

Show legend Show as text

Structure type: oligomer
Aglycon: (CH2)2NH
Trivial name: GT3
Compound class: CPS
Contained glycoepitopes: IEDB_130676,IEDB_130679,IEDB_136044,IEDB_136794,IEDB_137472,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150072,IEDB_150933,IEDB_150937,IEDB_153198,IEDB_153199,IEDB_190606,IEDB_558870,IEDB_858580,IEDB_983929,IEDB_983931,SB_116,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_35,SB_37,SB_39,SB_42,SB_6,SB_68,SB_7,SB_76,SB_84,SB_88

• Article ID: 4373
  Pincus SH, Moran E, Maresh G, Jennings HJ, Pritchard DG, Egan ML, Blixt O "Fine specificity and cross-reactions of monoclonal antibodies to group B streptococcal capsular polysaccharide type III" - Vaccine 30(32) (2012) 4849-4858
  

  Group B streptococcus (GBS) is a major cause of neonatal sepsis and meningitis. Despite aggressive campaigns using antenatal prophylactic antibiotic therapy, infections continue. Developing an effective maternal vaccine is a public health priority. Antibody (Ab) to the capsular polysaccharide (CPS) is considered the dominant 'protective' immune mediator. Here we study the fine specificity and potential host reactivity of a panel of well-characterized murine monoclonal Abs against the type III CPS by examining the binding of the Abs to intact and neuraminidase-digested GBS, purified CPS, synthetic carbohydrate structures, and cells. The results showed marked differences in the fine specificity among these mAbs to a single carbohydrate structure. Cross-reactions with synthetic GD3 and GT3 carbohydrates, representing structures found on surfaces of neural and developing cells, were demonstrated using carbohydrate array technology. The anti-CPS(III) mAbs did not react with cells expressing GD3 and GT3, nor did mAbs specific for the host carbohydrates cross-react with GBS, raising questions about the physiological relevance of this cross-reaction. But in the process of these investigations, we serendipitously demonstrated cross-reactions of some anti-CPS(III) mAbs with antigens, likely carbohydrates, found on human leukocytes. These studies suggest caution in the development of a maternal vaccine to prevent infection by this important human pathogen.

  capsular polysaccharide, group B Streptococcus, cross-reaction, monoclonal Ab, fine specificity, carbohydrate array

  NCBI PubMed ID: 22634296
  Publication DOI: 10.1016/j.vaccine.2012.05.006
  Journal NLM ID: 8406899
  Publisher: Elsevier
  Correspondence: spincus@chnola-research.org
  Institutions: Research Institute for Children, Children's Hospital, New Orleans, LA 70118, United States, Institute for Biological Sciences, National Research Council of Canada, Ottawa, ON, Canada K1A 0R6, Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL 35294, United States, Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, United States, Copenhagen Center for Glycomics (CCG), Departments of Cellular and Molecular Medicine and Dentistry, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, DK-2200, Copenhagen N, Denmark
  Methods: ELISA, biological assays, serological methods, binding assays, immunofluorescence analyses
  
   
  
  Streptococcus sp. B
  CSDB ID 28584 (all data & tools)

Show legend Show as text

Structure type: oligomer
Aglycon: lipid A
Trivial name: disialylated Hep3 glycoform
Compound class: LPS
Contained glycoepitopes: IEDB_115009,IEDB_116046,IEDB_120354,IEDB_123890,IEDB_130650,IEDB_130676,IEDB_130679,IEDB_136044,IEDB_136794,IEDB_137472,IEDB_137777,IEDB_137779,IEDB_138949,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_140624,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_144998,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_150937,IEDB_153198,IEDB_153199,IEDB_190606,IEDB_2189047,IEDB_241118,IEDB_858580,IEDB_983931,SB_116,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_35,SB_37,SB_39,SB_42,SB_6,SB_68,SB_7,SB_76,SB_84,SB_88

• Article ID: 4412
  Vitiazeva V, Li J, Hood DW, Richard ME, Schweda EK "The structural diversity of lipopolysaccharide expressed by non-typeable Haemophilus influenzae strains 1158 and 1159" - Carbohydrate Research 357 (2012) 98-110
  

  A heterogeneous population of glycoforms expressed by NTHi strains 1158 and 1159 has been elucidated using NMR spectroscopy and capillary electrophoresis coupled to electrospray ionization mass spectrometry (CE-ESI-MS) on O-deacylated LPS (LPS-OH) and core oligosaccharide (OS) materials, as well as HPLC-ESI-MS(n) on dephosphorylated and methylated OS samples. The most abundant glycoform contained a disaccharide chain: PCho→7)-D-α-D-Hepp-(1→6)-β-D-Glcp linked to HepI from the common structural element of H. influenzae LPS: L-α-D-HepIIIp-(1→2)-[PEtn→6]-L-α-D-HepIIp-(1→3)-L-α-D-HepIp-(1→5)-[PPEtn→4]-α-Kdop-(2→6)-lipid A. Phosphocholine (PCho) was found at two positions in the LPS glycoforms; PCho substituted the 6-position of β-D-Glcp attached to HepIII and was also located at a novel position linked to D-α-D-Hepp; this latter position was determined by structural analysis of LPS from a 1158lpsA mutant strain. Additionally, HPLC-ESI-MS(n) experiments indicated glycoforms that have chain elongation from HepII, this was found only in glycoforms, which lack the additional heptose in the outer core region. Structural details of these glycoforms were confirmed by analyses of LPS from a 1158losB2 mutant strain; the losB2 gene is required for addition of the D,D-Hep to the outer core region in strain 1158.

  Lipopolysaccharide, Haemophilus influenzae, Phosphocholine, globotetraose, sialyllactose

  NCBI PubMed ID: 22705099
  Publication DOI: 10.1016/j.carres.2012.04.023
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: E.K.H. Schweda
  Institutions: Clinical Research Centre, Karolinska Institutet, Novum, S-141 86 Huddinge, Sweden
  Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, HF solvolysis, sugar analysis, 31P NMR, ESI-MS, mild acid hydrolysis, de-O-acylation with hydrazine, NMR-1D, HPLC, CE-ESI-MS
  
   
  
  Haemophilus influenzae NTHi 1158, Haemophilus influenzae NTHi 1159
  CSDB ID 28679 (all data & tools)

Show legend Show as text

Structure type: oligomer
Aglycon: lipid A
Trivial name: disialylated Hep4 glycoform
Compound class: LPS
Contained glycoepitopes: IEDB_120354,IEDB_123890,IEDB_130650,IEDB_130676,IEDB_130679,IEDB_136044,IEDB_136794,IEDB_137472,IEDB_137777,IEDB_137779,IEDB_138949,IEDB_139428,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_150937,IEDB_153198,IEDB_153199,IEDB_190606,IEDB_2189046,IEDB_2189047,IEDB_858580,IEDB_983931,SB_116,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_35,SB_37,SB_39,SB_42,SB_6,SB_68,SB_7,SB_76,SB_84,SB_88

• Article ID: 4412
  Vitiazeva V, Li J, Hood DW, Richard ME, Schweda EK "The structural diversity of lipopolysaccharide expressed by non-typeable Haemophilus influenzae strains 1158 and 1159" - Carbohydrate Research 357 (2012) 98-110
  

  A heterogeneous population of glycoforms expressed by NTHi strains 1158 and 1159 has been elucidated using NMR spectroscopy and capillary electrophoresis coupled to electrospray ionization mass spectrometry (CE-ESI-MS) on O-deacylated LPS (LPS-OH) and core oligosaccharide (OS) materials, as well as HPLC-ESI-MS(n) on dephosphorylated and methylated OS samples. The most abundant glycoform contained a disaccharide chain: PCho→7)-D-α-D-Hepp-(1→6)-β-D-Glcp linked to HepI from the common structural element of H. influenzae LPS: L-α-D-HepIIIp-(1→2)-[PEtn→6]-L-α-D-HepIIp-(1→3)-L-α-D-HepIp-(1→5)-[PPEtn→4]-α-Kdop-(2→6)-lipid A. Phosphocholine (PCho) was found at two positions in the LPS glycoforms; PCho substituted the 6-position of β-D-Glcp attached to HepIII and was also located at a novel position linked to D-α-D-Hepp; this latter position was determined by structural analysis of LPS from a 1158lpsA mutant strain. Additionally, HPLC-ESI-MS(n) experiments indicated glycoforms that have chain elongation from HepII, this was found only in glycoforms, which lack the additional heptose in the outer core region. Structural details of these glycoforms were confirmed by analyses of LPS from a 1158losB2 mutant strain; the losB2 gene is required for addition of the D,D-Hep to the outer core region in strain 1158.

  Lipopolysaccharide, Haemophilus influenzae, Phosphocholine, globotetraose, sialyllactose

  NCBI PubMed ID: 22705099
  Publication DOI: 10.1016/j.carres.2012.04.023
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: E.K.H. Schweda
  Institutions: Clinical Research Centre, Karolinska Institutet, Novum, S-141 86 Huddinge, Sweden
  Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, HF solvolysis, sugar analysis, 31P NMR, ESI-MS, mild acid hydrolysis, de-O-acylation with hydrazine, NMR-1D, HPLC, CE-ESI-MS
  
   
  
  Haemophilus influenzae NTHi 1158, Haemophilus influenzae NTHi 1159
  CSDB ID 28680 (all data & tools)

Show legend Show as text

Structure type: structural motif or average structure
Trivial name: trisialoganglioside
Compound class: CPS
Contained glycoepitopes: IEDB_130648,IEDB_130676,IEDB_130678,IEDB_130679,IEDB_130680,IEDB_130681,IEDB_130683,IEDB_130684,IEDB_134627,IEDB_136044,IEDB_136794,IEDB_137339,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_147450,IEDB_147451,IEDB_149174,IEDB_150933,IEDB_150937,IEDB_153198,IEDB_153199,IEDB_190606,IEDB_433718,IEDB_547903,IEDB_858580,IEDB_983931,SB_116,SB_13,SB_14,SB_144,SB_164,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_2,SB_22,SB_23,SB_24,SB_25,SB_28,SB_3,SB_35,SB_37,SB_39,SB_4,SB_41,SB_42,SB_5,SB_6,SB_68,SB_7,SB_70,SB_71,SB_76,SB_8,SB_84,SB_88,SB_94,SB_95,SB_96,SB_97,SB_98

• Article ID: 4430
  Ovodov YS "Bacterial capsular antigens. Structural patterns of capsular antigens" - Biochemistry (Moscow) 71(9) (2006) 937-954
  

  Structural patterns of bacterial capsular antigens including capsular polysaccharides and exoglycans are given in this review. In addition, the immunological activity of capsular antigens and their role in type specificity of bacteria are discussed.

  structure, capsular polysaccharides, bacterial capsular antigens, bacterial exoglycans, immunological activity, type specificity

  NCBI PubMed ID: 17009947
  Publication DOI: 10.1134/S000629790609001X
  Journal NLM ID: 0376536
  Publisher: Nauka/Interperiodica
  Correspondence: ovoys@physiol.komisc.ru
  Institutions: Institute of Physiology, Komi Science Center, Urals Branch of the Russian Academy of Sciences, Syktyvkar 167982, Russia
  
   
  
  Escherichia coli K1
  CSDB ID 28748 (all data & tools)

Show legend Show as text

Structure type: fragment of a bigger structure
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130676,IEDB_130679,IEDB_136044,IEDB_136794,IEDB_137472,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_150937,IEDB_153198,IEDB_153199,IEDB_190606,IEDB_858580,IEDB_983931,SB_116,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_35,SB_37,SB_39,SB_42,SB_6,SB_68,SB_7,SB_76,SB_84,SB_88

• Article ID: 6049
  Di Lorenzo F, Duda KA, Lanzetta R, Silipo A, De Castro C, Molinaro A "A Journey from Structure to Function of Bacterial Lipopolysaccharides" - Chemical Reviews (2021)
  

  Lipopolysaccharide (LPS) is a crucial constituent of the outer membrane of most Gram-negative bacteria, playing a fundamental role in the protection of bacteria from environmental stress factors, in drug resistance, in pathogenesis, and in symbiosis. During the last decades, LPS has been thoroughly dissected, and massive information on this fascinating biomolecule is now available. In this Review, we will give the reader a third millennium update of the current knowledge of LPS with key information on the inherent peculiar carbohydrate chemistry due to often puzzling sugar residues that are uniquely found on it. Then, we will drive the reader through the complex and multifarious immunological outcomes that any given LPS can raise, which is strictly dependent on its chemical structure. Further, we will argue about issues that still remain unresolved and that would represent the immediate future of LPS research. It is critical to address these points to complete our notions on LPS chemistry, functions, and roles, in turn leading to innovative ways to manipulate the processes involving such a still controversial and intriguing biomolecule.

  Lipopolysaccharide, LPS, structure, Pathogenesis, carbohydrate, function, gram negative bacteria

  NCBI PubMed ID: 34286971
  Publication DOI: 10.1021/acs.chemrev.0c01321
  Journal NLM ID: 2985134R
  Publisher: Chem Rev
  Correspondence: Antonio Molinaro
  Institutions: Department of Chemical Sciences, University of Naples Federico II, via Cinthia 4, 80126 Naples, Italy, Task Force on Microbiome Studies, University of Naples Federico II, Via Cinthia 4, 80126 Naples, Italy, Research Center Borstel Leibniz Lung Center, Parkallee 4a, 23845 Borstel, Germany, Department of Agricultural Sciences, University of Naples Federico II, Via Universita 96, 80055 Portici, Naples, Italy, Department of Chemistry, School of Science, Osaka University, 1-1 Osaka University Machikaneyama, Toyonaka, Osaka 560-0043, Japan
  
   
  
  Haemophilus influenzae
  CSDB ID 7915 (all data & tools)