Lipoteichoic acid (LTA) is an important cell envelope compound of Gram-positive bacteria. LTA isolated from allergy-protective Staphylococcus sciuri W620 strain was characterized by chemical analyses as well as 1D and 2D NMR experiments. Compositional analyses indicated the presence of glycerol (Gro), phosphate-Gro, alanine-Gro, glucose (Glc) and fatty acids. The studied strain produced LTA with backbone composed of glycerol-phosphate repeating units only substituted with d-alanine (Ala) and the lipid anchor, typically for genus Staphyloccocus, possessing the structure β-d-Glcp(1→6)-β-d-Glcp(1→3)-1,2-diacyl-sn-Gro.
structure, NMR analysis, lipoteichoic acid, LTA structure, Allergy-protection, Staphylococcus sciuri
NCBI PubMed ID: 27196311Publication DOI: 10.1016/j.carres.2016.04.026Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: kduda@fz-borstel.de
Institutions: Junior Research Group Allergobiochemistry, Division of Structural Biochemistry, Priority Area Asthma and Allergies, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Parkallee 4a/c, D-23845 Borstel, Germany
Methods: 13C NMR, 1H NMR, NMR-2D, GLC-MS, de-O-acylation, 31P NMR, acid hydrolysis, GLC, composition analysis, NMR-1D, methanolysis, HPLC
Found