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1. Compound ID: 1466
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a-D-Fucp3NAc-(1-2)-+
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a-D-Fucp3NAc-(1-2)-+ |
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Me-3)-{{{-a-D-Rhap-(1-3)-a-D-Rhap-(1-2)-a-D-Rhap-(1-2)-a-D-Rhap-(1-3)-}}}/n=16/-{{{-a-D-Rhap-(1-3)-}}}/n=1-3/-b-D-GalpNAc-(1--/Thr67 of S-layer protein/ |
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Structure type: oligomer
Aglycon: Thr67 of S-layer protein
Compound class: S-layer glycoprotein
Contained glycoepitopes: IEDB_130648,IEDB_137473,IEDB_1394181,IEDB_144827,IEDB_145005,IEDB_145006,IEDB_145010
The structure is contained in the following publication(s):
- Article ID: 467
Schäffer C, Messner P "Surface-layer glycoproteins: an example for the diversity of bacterial glycosylation with promising impacts on nanobiotechnology" -
Glycobiology 14(8) (2004) 31R-42R
Bacterial cell surface layers, referred to simply as S-layers, have been described for all major phylogenetic groups of bacteria, which may indicate their pivotal role for a bacterium in its natural habitat. They have the unique ability to assemble into two-dimensional crystalline arrays that completely cover the bacterial cells. Glycosylation represents the most frequent modification of S-layer proteins. S-layer glycoproteins constitute a class of glycoconjugates first isolated in the mid-1970s, but S-layer glycoprotein research is still being regarded as an 'exotic field of glycobiology,' possibly because of its 'noneukaryotic' character. Extensive work over the past 30 years provided evidence of an enormous diversity of S-layer glycoproteins that have been created in nature over 3 billion years of prokaryotic evolution. These glycoconjugates are substantially different from eukaryotic glycoproteins, with regard to both composition and structure; nevertheless, some general structural concepts may be deduced. The awareness of the high application potential of S-layer glycoproteins, especially in combination with their intrinsic cell surface display feature, in the field of modern nanobiotechnology as a base for glycoengineering has recently led to the investigation of the S-layer protein glycosylation process at the molecular level, which has lagged behind the structural studies due to the lack of suitable molecular tools. From that work an even more interesting picture of this class of glycoconjugates is emerging. The availability of purified enzymes from S-layer glycan biosynthesis pathways exhibiting increased stabilities and/or rare sugar specificities in conjunction with preliminary genomic data on S-layer glycan biosynthesis clusters will pave the way for the rational design of S-layer neoglycoproteins.
LPS, bacterial glycosylation, genomic glycosylation loci, glycan diversity, glycoengineering, S-layer nanoglycobiology
NCBI PubMed ID: 15044388Publication DOI: 10.1093/glycob/cwh064Journal NLM ID: 9104124Publisher: IRL Press at Oxford University Press
Correspondence: paul.messner@boka.ac.at
Institutions: Center for NanoBiotechnology, University of Applied Life Sciences and Natural Resources, Gregor-Mendel-Strasse 33, A-1180 Wien, Austria
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2. Compound ID: 1482
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a-D-Fucp3NAc-(1-2)-+
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a-D-Fucp3NAc-(1-2)-+ |
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-3)-a-D-Rhap-(1-3)-a-D-Rhap-(1-2)-a-D-Rhap-(1-2)-a-D-Rhap-(1- |
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Structure type: polymer chemical repeating unit
; n=15
Trivial name: S-layer glycoprotein
Contained glycoepitopes: IEDB_1394181,IEDB_144827,IEDB_145005,IEDB_145006,IEDB_145010
The structure is contained in the following publication(s):
- Article ID: 470
Novotny R, Pfoestl A, Messner P, Schäffer C "Genetic organization of chromosomal S-layer glycan biosynthesis loci of Bacillaceae" -
Glycoconjugate Journal 20(7-8) (2004) 435-447
S-layer glycoproteins are cell surface glycoconjugates that have been identified in archaea and in bacteria. Usually, S-layer glycoproteins assemble into regular, crystalline arrays covering the entire bacterium. Our research focuses on thermophilic Bacillaceae, which are considered a suitable model system for studying bacterial glycosylation. During the past decade, investigations of S-layer glycoproteins dealt with the elucidation of the highly variable glycan structures by a combination of chemical degradation methods and nuclear magnetic resonance spectroscopy. It was only recently that the molecular characterization of the genes governing the formation of the S-layer glycoprotein glycan chains has been initiated. The S-layer glycosylation (slg) gene clusters of four of the 11 known S-layer glycan structures from members of the Bacillaceae have now been studied. The clusters are approximately 16 to approximately 25 kb in size and transcribed as polycistronic units. They include nucleotide sugar pathway genes that are arranged as operons, sugar transferase genes, glycan processing genes, and transporter genes. So far, the biochemical functions only of the genes required for nucleotide sugar biosynthesis have been demonstrated experimentally. The presence of insertion sequences and the decrease of the G + C content at the slg locus suggest that the investigated organisms have acquired their specific S-layer glycosylation potential by lateral gene transfer. In addition, S-layer protein glycosylation requires the participation of housekeeping genes that map outside the cluster. The gene encoding the respective S-layer target protein is transcribed monocistronically and independently of the slg cluster genes. Its chromosomal location is not necessarily in close vicinity to the slg gene cluster.
bacterial glycosylation, S-layer, glycosylation gene cluster, sugar nucleotides, S-layer gene, glycan biosynthesis
NCBI PubMed ID: 15316277Publication DOI: 10.1023/B:GLYC.0000038290.74944.65Journal NLM ID: 8603310Publisher: Kluwer Academic Publishers
Institutions: University of Applied Life Sciences and Natural Resources, Center for NanoBiotechnology, Gregor-Mendel Strasse 33, A-1180 Wien, Austria
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3. Compound ID: 1483
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a-D-Fucp3NAc-(1-2)-+
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a-D-Fucp3NAc-(1-2)-+ |
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Me-3)-{{{-a-D-Rhap-(1-3)-a-D-Rhap-(1-2)-a-D-Rhap-(1-2)-a-D-Rhap-(1-3)-}}}/n=16/-{{{-a-D-Rhap-(1-3)-}}}/n=0-2/-a-D-Rhap-(1-3)-b-D-GalpNAc-(1-3)-Thr-(?--/Thr-67 in S-layer protein/ |
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Structure type: oligomer
Aglycon: Thr-67 in S-layer protein
Trivial name: S-layer glycoprotein
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130648,IEDB_137473,IEDB_1394181,IEDB_144827,IEDB_145005,IEDB_145006,IEDB_145010
The structure is contained in the following publication(s):
- Article ID: 470
Novotny R, Pfoestl A, Messner P, Schäffer C "Genetic organization of chromosomal S-layer glycan biosynthesis loci of Bacillaceae" -
Glycoconjugate Journal 20(7-8) (2004) 435-447
S-layer glycoproteins are cell surface glycoconjugates that have been identified in archaea and in bacteria. Usually, S-layer glycoproteins assemble into regular, crystalline arrays covering the entire bacterium. Our research focuses on thermophilic Bacillaceae, which are considered a suitable model system for studying bacterial glycosylation. During the past decade, investigations of S-layer glycoproteins dealt with the elucidation of the highly variable glycan structures by a combination of chemical degradation methods and nuclear magnetic resonance spectroscopy. It was only recently that the molecular characterization of the genes governing the formation of the S-layer glycoprotein glycan chains has been initiated. The S-layer glycosylation (slg) gene clusters of four of the 11 known S-layer glycan structures from members of the Bacillaceae have now been studied. The clusters are approximately 16 to approximately 25 kb in size and transcribed as polycistronic units. They include nucleotide sugar pathway genes that are arranged as operons, sugar transferase genes, glycan processing genes, and transporter genes. So far, the biochemical functions only of the genes required for nucleotide sugar biosynthesis have been demonstrated experimentally. The presence of insertion sequences and the decrease of the G + C content at the slg locus suggest that the investigated organisms have acquired their specific S-layer glycosylation potential by lateral gene transfer. In addition, S-layer protein glycosylation requires the participation of housekeeping genes that map outside the cluster. The gene encoding the respective S-layer target protein is transcribed monocistronically and independently of the slg cluster genes. Its chromosomal location is not necessarily in close vicinity to the slg gene cluster.
bacterial glycosylation, S-layer, glycosylation gene cluster, sugar nucleotides, S-layer gene, glycan biosynthesis
NCBI PubMed ID: 15316277Publication DOI: 10.1023/B:GLYC.0000038290.74944.65Journal NLM ID: 8603310Publisher: Kluwer Academic Publishers
Institutions: University of Applied Life Sciences and Natural Resources, Center for NanoBiotechnology, Gregor-Mendel Strasse 33, A-1180 Wien, Austria
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4. Compound ID: 2908
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b-D-GlcpNAc-(1-4)-+
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-3)-a-D-Rhap-(1-3)-a-D-Rhap-(1-2)-a-D-Rhap-(1-2)-a-D-Rhap-(1- |
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Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_1394181,IEDB_141807,IEDB_144827,IEDB_145005,IEDB_145006,IEDB_145008,IEDB_145010,IEDB_151531
The structure is contained in the following publication(s):
- Article ID: 1038
Müller P, Zähringer U, Rudolph K "Induced resistance by bacterial lipopolysaccharides (LPS)" -
Proceedings of Plant Pathogenic Bacteria, International Conference (9th : 1996 : Madras, India) (1998) 569-575
Lipopolysaccharides (LPS) were extracted from the Pseudomonas syringae pathovars: tomato race 1 (GSPB Nr. 1778), tomato race 0 (GSPB Nr. 1776), and glycinea race 9 (GSPB Nr. 1986) by the phenol/chloroform/petroleumbenzene-method (Galanos -et al 1979). The chemical analysis revealed 10% contamination by levan, a polyfructose, but also high amounts of KDO, PO4 (3-), GlcNAc and rhamnose. Chemical structure-analysis identified the O-specific chain, the-core region and lipid A. Lipid A was composed of the typical fatty acids of Pseudomonas such as 3-OH-C 10:0, C12:0, 3-OH C 12:0 and 2-OH C12:0. The structure of the O-chain corresponded to the one reported by Zdovorvenko et al. 1992, typical for Pseudomonas syringae pv. glycinea. Determination of the core-region has not been completely finished, but seems to show strong similarities to Pseudomonas aeruginosa and P. fluorescens. Induced resistance was studied in tomato and tobacco leaves. We investigated the role of whole LPS as well as its subunits O-chain and core region on induced resistance in compatible and incompatible systems. Resistance was induced by 50 jig LPS/ml in the incompatible system (LPS of Pseudomonas syringae pv. glycinea race 9 against P. s. pv. tomato race 1 in tomato cv. "Lyconorma"). Neither the O-specific chain nor the core region alone induced resistance. When similar experiments were performed by pretreatment with P.s. pv. tomato LPS followed by inoculation with P. s. pv. tomato (compatible system) resistance was not induced. Tobacco leaves were pretreated with 50-1000 uxj LPS/ml of P. s. pv. glycinea race 9 followed by inoculation with 108cfu/ml P. s. pv. glycinea race 9 48 h later. The pretreatment delayed appearance of the HR from 20 to 60 hrs after bacterial inoculation. The O-chain or the coreregion did not cause this effect. Further experiments have to show why the plants can differentiate between compatible and incompatible LPS, and whether the complete LPS-molecule is necessary for resistance induction or whether lipid A alone can cause this effect.
Lipopolysaccharide, lipopolysaccharides, LPS, structure, core, Bacterial, lipid A, induced, O polysaccharide, bacteria, Pseudomonas syringae, composition, resistance, plant, pathogenic bacteria, pathogenic, HR
Correspondence: PMUELLE@GWDG.DE
Editors: Mahadevan A
Institutions: Institut fur Phytopathologie and Pflanzenschutz der Universitat Gottingen Grisebachstr. 6, D-37077 Gottingen, Germany, Zentrum fur Medizin and Biowissenschaften, Borstel, Germany
Methods: sugar analysis
- Article ID: 1777
Knirel YA, Kochetkov NK "The structure of lipopolysaccharides of gram-negative bacteria. III. The structure of O-antigens: A review" -
Biochemistry (Moscow) 59(12) (1994) 1325-1383
This review summarizes data on the composition and structure of the O-antigens, the polysaccharide chains of the outer-membrane lipopolysaccharides (LPS) of Gram-negative bacteria defining the immunospecificity of these microbial cells. Special reference is given to some structural features of the O-antigens, such as the presence of unique monosaccharides and noncarbohydrate components, masked regularity, and the occurrence in one microorganism of LPS with structurally different polysaccharide chains. Antigenic relationships between microorganisms belonging to different taxonomic groups are discussed.
structure, O-antigen, chemical composition, bacterial lipopolysaccharides, Salmonella livingstone C1
NCBI PubMed ID: 7533007Journal NLM ID: 0376536Publisher: Nauka/Interperiodica
Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
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5. Compound ID: 2933
Structure type: polymer chemical repeating unit
Trivial name: D-rhamnan
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_1394181,IEDB_144827,IEDB_145005,IEDB_145006,IEDB_145010
The structure is contained in the following publication(s):
- Article ID: 1071
Ovod V, Rudolph K, Knirel YA, Krohn K "Immunochemical characterization of O polysaccharides composing the a-D-rhamnose backbone of lipopolysaccharide of Pseudomonas syringae and classification of bacteria into serogroups O1 and O2 with monoclonal antibodies" -
Journal of Bacteriology 178 (1996) 6459-6465
Murine monoclonal antibodies (MAbs) reacting with Pseudomonas syringae lipopolysaccharide (LPS) O polysaccharides (OPS) composed of tetra- and tri-α-D-rhamnose repeats in the backbone [3)D-Rha(α1-3)D-Rha(α1-2)D-Rha(α1-2)D-Rha(α1] and [3)D-Rha(α1-3)D-Rha(α1-2)D-Rha(α1] were generated and used for immunochemical analysis and for serological classification of the bacteria. A total of 195 of 358 P. syringae strains tested representing 21 pathovars were shown to share a common epitope, 1a, and were classified into serogroup O1. All strains with pathovars aptata, glycinea, japonica, phaseolicola, and pisi, most of the strains with pathovars atrofaciens and striafaciens, and half of the strains with pathovar syringae were classified into serotypes O1a', O1b, O1c, and O1d within serogroup O1. Serogroup-specific epitope 1a was inferred to be related to the (α1-2)D-Rha(α1-3) site of the OPS backbone. The serotype-specific epitopes 1b, 1c, 1d, and 1a' were inferred as relating to the immunodominant lateral (α1-3)D-Rha, (β1-4)D-GlcNAc, and (α1-4)D-Fuc substituents and backbone-located site (α1-3)D-Rha(α1-2), respectively, of OPSs that share the common tetra-D-rhamnose repeats in the backbone. A total of 7.3% of the strains studied, all with pathovars morsprunorum and lapsa, were classified as serotypes O2a and O2d within serogroup 02. Serotype-specific epitope 2a was inferred as being related to the backbone-located site D-Rha(α1-3)D-Rha and epitope 2d to the immunodominant lateral (α1-4)D-Fuc residue of OPS consisting of tri-D-rhamnose repeats in the backbone. Epitope 2d alternated with 2a within the same LPS molecule and did not cross-react with epitope 1d. Serotypes O2a and O2d were observed in some strains correlating with the coexpression of the two chemotypes of OPS by the same strain. The serogroup O1-specific MAb Ps1a reacted weakly but definitely with all strains from serogroup 02. We propose serological formulas for serogroups O1 and 02 as well as for individual strains within these serogroups.
Lipopolysaccharide, LPS, characterization, polysaccharide, polysaccharides, Pseudomonas, antibodies, antibody, epitope, monoclonal, monoclonal antibodies, monoclonal antibody, O-polysaccharide, O polysaccharide, bacteria, serogroup, backbone, immunochemical, Pseudomonas syringae, classification, D-rhamnose
NCBI PubMed ID: 8932301Journal NLM ID: 2985120RPublisher: American Society for Microbiology
Correspondence: Ltvlov@uta.fi
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Medical Technology, University of Tampere, Tampere, Finland, Department of Microbiology and Immunology, University of Kiev, Kiev, Ukraine, Institut fur Pflanzenpathologie und Pflanzenschutz der Georg-August-Universitat, Gottingen, Germany
Methods: serological methods
- Article ID: 1073
Ovod V, Knirel Y, Krohn K "Demonstration of the immunochemical diversity of O-chains of lipopolysaccharide of Pseudomonas syringae and inferring of the serogroup- and serotype-specific epitopes with monoclonal antibodies" -
Proceedings of International Conference on Pseudomonas syringae Pathovars and Related Pathogens (5th : 1995 : Berlin, Germany) (1997) Vol. 9, 532-537
Using serogroup- and serotyppe-specific murine monoclonal antibodies (MAbs) to Pseudomonas syringae lipopolysacharide (LPS) O-polysaccharides (OPS) (=O chains) with elucidated primary chemical structure of the O-repeating units, a rather high diversity of the OPS-related epitopes was demonstrated and most of them were inferred. The immunogenic properties of the O-serogroup- and O-serotype-specific epitopes were shown to depend on the nature and the number of sugar residues in the O-repeat as well as on the arrangement of the monosaccharides and the mode of linkages between them.
Lipopolysaccharide, LPS, structure, strain, Pseudomonas, chain, group, antibodies, antibody, epitope, monoclonal, monoclonal antibodies, monoclonal antibody, epitopes, O-polysaccharide, serogroup, immunochemical, O-chain, pathogen, pathogens, pathovar, Pseudomonas syringae, classification, diversity, serotype-specific
Publisher: Kluwer Academic Publishers, The Netherlands
Correspondence: knirel@ioc.ac.ru
Editors: Rudolph K, Burr TJ, Mansfield JW, Stead DE, Vivian A, von Kietzell J
Institutions: Institute of Medical Technology, University of Tampere, Tampere, Finland, N.D. Zelinsy Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
- Article ID: 1074
Ovod V, Knirel YA, Samson R, Krohn K "Immunochemical characterization and taxonomic evaluation of the O polysaccharides of the lipopolysaccharides of Pseudomonas syringae serogroup O1 strains" -
Journal of Bacteriology 181(22) (1999) 6937-6947
The O polysaccharide (OPS) of the lipopolysaccharide (LPS) of Pseudomonas syringae pv. atrofaciens IMV 7836 and some other strains that are classified in serogroup O1 was shown to be a novel linear α-D-rhamnan with the tetrasaccharide O repeat →3)-α-D-Rhap-(1→3)-α-D-Rhap-(1→2)-α-D-Rhap-(1→2)-α-D-Rhap-(1→ (chemotype 1A). The same α-D-rhamnan serves as the backbone in branched OPSs with lateral (α1→3)-linked D-Rhap, (β1→4)-linked D-GlcpNAc, and (α1→4)-linked D-Fucf residues (chemotypes 1B, 1C, and 1D, respectively). Strains of chemotype 1C demonstrated variations resulting in a decrease of the degree of substitution of the backbone 1A with the lateral D-GlcNAc residue (chemotype 1C-1A), which may be described as branched regular <→ branched irregular → linear OPS structure alterations (1C <→ 1C-1A → 1A). Based on serological data, chemotype 1D was suggested to undergo a 1D <→ 1D-1A alteration, whereas chemotype 1B showed no alteration. A number of OPS backbone-specific monoclonal antibodies (MAbs), Ps(1-2)a, Ps(1-2)a(1), Ps1a, Ps1a(1), and Ps1a(2), as well as MAbs Ps1b, Ps1c, Ps1c(1), Ps1d, Ps(1-2)d, and Ps(1-2)d(1) specific to epitopes related to the lateral sugar substituents of the OPSs, were produced against P. syringae serogroup O1 strains. By using MAbs, some specific epitopes were inferred, serogroup O1 strains were serotyped in more detail, and thus, the serological classification scheme of P. syringae was improved. Screening with MAbs of about 800 strains representing all 56 known P. syringae pathovars showed that the strains classified in serogroup O1 were found among 15 pathovars and the strains with the linear OPSs of chemotype 1A were found among 9 of the 15 pathovars. A possible role for the LPS of P. syringae and related pseudomonads as a phylogenetic marker is discussed.
Lipopolysaccharide, lipopolysaccharides, LPS, strain, structural, characterization, polysaccharide, polysaccharides, Pseudomonas, antibody, epitope, monoclonal, monoclonal antibody, O-polysaccharide, O polysaccharide, bacteria, serogroup, evaluation, backbone, immunochemical, Pseudomonas syringae, classification, linear, taxonomic, D-rhamnose
NCBI PubMed ID: 10559159Journal NLM ID: 2985120RPublisher: American Society for Microbiology
Correspondence: Ltvlov@uta.fi
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Medical Technology, University of Tampere, Tampere, Finland, Pathologie Vegetale, Institut National de la Recherche Agronomique, Beaucouze Cedex, France
Methods: NMR
- Article ID: 1313
Zdorovenko EL, Ovod V, Zatonsky GV, Shashkov AS, Kocharova NA, Knirel YA "Location of the O-methyl groups in the O polysaccharide of Pseudomonas syringae pv. phaseolicola" -
Carbohydrate Research 330(4) (2001) 505-510
The O-methylation pattern of the O polysaccharide (OPS) of the lipopolysaccharide of Pseudomonas syringae pv. phaseolicola GSPB 1552 was revealed by methylation (CD3I) analysis, Smith degradation, and NMR spectroscopy. Together with the major O repeats consisting of D-rhamnopyranose (D-Rhap) and D-fucofuranose (D-Fucf), there are minor repeats (approximately 30%) containing 3-O-methyl-D-rhamnose (D-acofriose), which is 2-substituted in the interior repeats and occupies the terminal non-reducing end of the OPS. It was suggested that the methylated O repeats are linked to each other nearby the non-reducing end of the OPS and that the 'biological' O repeat of the OPS has the following structure: [molecular structure: see text]
Lipopolysaccharide, Pseudomonas syringae, O-polysaccharides, O-methylation, phytopathogens
NCBI PubMed ID: 11269402Publication DOI: 10.1016/S0008-6215(01)00005-2Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: knirel@ioc.ac.ru
Institutions: Institute of Medical Technology, University of Tampere, Tampere, Finland, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Institute of Medical Technology, University of Tampere, Tampere, Finalnd
Methods: NMR-2D, methylation, NMR, Smith degradation
- Article ID: 1452
Corsaro MM, De Castro C, Molinaro A, Parrilli M "Structure of lipopolysaccharides from phytopathogenic Gram-negative bacteria" -
Book: Recent Research Developments in Phytochemistry (2001) Vol. 5, 119-138
This review collects the structural data of lipopolysaccharide components arising from all phytopathogenic bacteria so far investigated. The structural approaches and the main biological role of these macromolecules are also reported.
Lipopolysaccharide, lipopolysaccharides, structure, core, lipid A, O-polysaccharide, gram negative bacteria
WWW link: https://books.google.ru/books/about/Recent_Research_Developments_in_Phytoche.html?id=5CJacgAACAAJ&redir_esc=yPublisher: Research Signpost, Trivandrum, India
Editors: Pandalai SG
Institutions: Dipartimento di Chimica Organica e Biochimica, Complesso Universitario Monte S.Angelo Via Cintia, 4, 80126 Napoli, Italy
- Article ID: 1465
Knirel YA, Zdorovenko GM "Structures of O-polysaccharide chains of lipopolysaccharides as the basis for classification of Pseudomonas syringae and related strains" -
Book: Pseudomonas Syringae Pathovars and Related Pathogens (series: Developments in Plant Pathology) (1997) 475-480
The O-polysaccharides of various serogroups of P. syringae were found to have similar structures with the main chain of a rhamnan which may carry a monosaccharide side chain of D-rhamnose, D-fucose, 2-acetamido-2-deoxy-D-glucose or 3-acetamido-3,6-dideoxy-D-galactose. The relationship between the serological specificity and the host-plant specificity of P. syringae and the structures of the O-polysaccharides is discussed.
Lipopolysaccharide, structure, O-antigen, O-polysaccharide, serological specificity, Pseudomonas syringae, Serogrouping, Host-plant specificity
Publication DOI: 10.1007/978-94-011-5472-7_85Publisher: Springer Netherlands
Editors: Rudolph K, Burr TJ, Mansfield JW, Stead D, Vivian A, von Kietzell J
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Leninsky Pr. 47, Moscow B-334, Russia, D.K. Zabolotny Institute of Microbiology and Virology, Zabolotnogo 154, Kiev-143, Ukraine
- Article ID: 1853
Knirel YA, Zdorovenko GM, Shashkov AS, Gubanova NY, Yakovleva LM, Gvozdyak RI "Antigenic polysaccharides of bacteria. 27. Structure of the O-specific polysaccharide chain of lipopolysaccharides from Pseudomonas syringae pvs atrofaciens 2399, phaseolicola 120a and Pseudomonas holci 8299, belonging to serogroup VI" -
Bioorganicheskaya Khimia = Bioorganic Chemistry [Russian] 14(1) (1988) 92-99
Lipopolysaccharides from Pseudomonas syringae pvs atrofaciens 2399. phaseolicola 120a and Pseudomonas holci 8299, belonging to serogroup VI. possess an identical polysaccharide chain composed of D-rhamnose and D-fucose. On the hasis of methylation, partial acid hydrolysis, 1H- and 13C-NMR data, it was concluded that the backbone of the polysaccharide represents D-rhamnan built up of tetrasaccharide repeating units and α-D-fucofuranose residues are attached to the backbone as the monosaccharide branches. The following structure of the repeating unit is established: (Formula: see text).
NCBI PubMed ID: 2454626Journal NLM ID: 7804941WWW link: http://www.rjbc.ru/arc/14/1/0092-0099.pdfPublisher: Moskva: Nauka
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the USSR, Moscow, Russia
Methods: 13C NMR, 1H NMR
- Article ID: 2789
Ovod V, Ashorn P, Yakovleva L, Krohn K "Classification of Pseudomonas syringae with monoclonal antibodies against the core and O-side chains of the lipopolysaccharide" -
Phytopathology 85 (1995) 226-232
Journal NLM ID: 9427222Publisher: American Phytopathological Society
- Article ID: 3968
Zdorovenko GM, Zdorovenko EL "Pseudomonas syringae lipopolysaccharides: Immunochemical characteristics and structure as a basis for strain classification" -
Mikrobiologiia = Microbiology [Russian] 79(1) (2010) 47-57
Lipopolysaccharide (LPS) preparations of 34 Pseudomonas syringae strains of 19 pathovars were prepared by saline extraction from wet cells and purified by repeated ultracentrifugation. The preparations reacted with homologous O-antisera, obtained by rabbit immunization with heat-killed bacterial cells. Through inhibition of homologous reactions between LPS preparations of heterologous strains (enzyme immunoassay, EIA), it was established for the first time that high serological affinity between strains is observed only if their LPS contains O-specific polysaccharide chains (OPS) comprised of completely identical rather than partially similar units. The central linear part of the OPS was found to be serologically inert when shielded with side groups. Data on immunochemical characteristics of the LPS and OPS structure are analyzed in relation to the design of P. syringae classification scheme.
Lipopolysaccharide, structure, O-specific polysaccharide, Pseudomonas syringae, classification, immunochemistry
NCBI PubMed ID: 20411661Publication DOI: 10.1134/S0026261710010078Journal NLM ID: 0376652Publisher: Moskva: Izdatelstvo Nauka
Correspondence: evelina@ioc.ac.ru
Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Zabolotnyi Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, ul. Zabolotnogo 154, Kyiv, 03143 Ukraine
Methods: partial acid hydrolysis, EIA, serological methods, de-N-acetylation/deamination
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6. Compound ID: 2978
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a-D-Rhap-(1-3)-+
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-3)-a-D-Rhap-(1-3)-a-D-Rhap-(1-2)-a-D-Rhap-(1-2)-a-D-Rhap-(1- |
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Structure type: polymer chemical repeating unit
Trivial name: D-rhamnan
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_1394181,IEDB_144827,IEDB_144828,IEDB_145005,IEDB_145006,IEDB_145007,IEDB_145010
The structure is contained in the following publication(s):
- Article ID: 1071
Ovod V, Rudolph K, Knirel YA, Krohn K "Immunochemical characterization of O polysaccharides composing the a-D-rhamnose backbone of lipopolysaccharide of Pseudomonas syringae and classification of bacteria into serogroups O1 and O2 with monoclonal antibodies" -
Journal of Bacteriology 178 (1996) 6459-6465
Murine monoclonal antibodies (MAbs) reacting with Pseudomonas syringae lipopolysaccharide (LPS) O polysaccharides (OPS) composed of tetra- and tri-α-D-rhamnose repeats in the backbone [3)D-Rha(α1-3)D-Rha(α1-2)D-Rha(α1-2)D-Rha(α1] and [3)D-Rha(α1-3)D-Rha(α1-2)D-Rha(α1] were generated and used for immunochemical analysis and for serological classification of the bacteria. A total of 195 of 358 P. syringae strains tested representing 21 pathovars were shown to share a common epitope, 1a, and were classified into serogroup O1. All strains with pathovars aptata, glycinea, japonica, phaseolicola, and pisi, most of the strains with pathovars atrofaciens and striafaciens, and half of the strains with pathovar syringae were classified into serotypes O1a', O1b, O1c, and O1d within serogroup O1. Serogroup-specific epitope 1a was inferred to be related to the (α1-2)D-Rha(α1-3) site of the OPS backbone. The serotype-specific epitopes 1b, 1c, 1d, and 1a' were inferred as relating to the immunodominant lateral (α1-3)D-Rha, (β1-4)D-GlcNAc, and (α1-4)D-Fuc substituents and backbone-located site (α1-3)D-Rha(α1-2), respectively, of OPSs that share the common tetra-D-rhamnose repeats in the backbone. A total of 7.3% of the strains studied, all with pathovars morsprunorum and lapsa, were classified as serotypes O2a and O2d within serogroup 02. Serotype-specific epitope 2a was inferred as being related to the backbone-located site D-Rha(α1-3)D-Rha and epitope 2d to the immunodominant lateral (α1-4)D-Fuc residue of OPS consisting of tri-D-rhamnose repeats in the backbone. Epitope 2d alternated with 2a within the same LPS molecule and did not cross-react with epitope 1d. Serotypes O2a and O2d were observed in some strains correlating with the coexpression of the two chemotypes of OPS by the same strain. The serogroup O1-specific MAb Ps1a reacted weakly but definitely with all strains from serogroup 02. We propose serological formulas for serogroups O1 and 02 as well as for individual strains within these serogroups.
Lipopolysaccharide, LPS, characterization, polysaccharide, polysaccharides, Pseudomonas, antibodies, antibody, epitope, monoclonal, monoclonal antibodies, monoclonal antibody, O-polysaccharide, O polysaccharide, bacteria, serogroup, backbone, immunochemical, Pseudomonas syringae, classification, D-rhamnose
NCBI PubMed ID: 8932301Journal NLM ID: 2985120RPublisher: American Society for Microbiology
Correspondence: Ltvlov@uta.fi
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Medical Technology, University of Tampere, Tampere, Finland, Department of Microbiology and Immunology, University of Kiev, Kiev, Ukraine, Institut fur Pflanzenpathologie und Pflanzenschutz der Georg-August-Universitat, Gottingen, Germany
Methods: serological methods
- Article ID: 1073
Ovod V, Knirel Y, Krohn K "Demonstration of the immunochemical diversity of O-chains of lipopolysaccharide of Pseudomonas syringae and inferring of the serogroup- and serotype-specific epitopes with monoclonal antibodies" -
Proceedings of International Conference on Pseudomonas syringae Pathovars and Related Pathogens (5th : 1995 : Berlin, Germany) (1997) Vol. 9, 532-537
Using serogroup- and serotyppe-specific murine monoclonal antibodies (MAbs) to Pseudomonas syringae lipopolysacharide (LPS) O-polysaccharides (OPS) (=O chains) with elucidated primary chemical structure of the O-repeating units, a rather high diversity of the OPS-related epitopes was demonstrated and most of them were inferred. The immunogenic properties of the O-serogroup- and O-serotype-specific epitopes were shown to depend on the nature and the number of sugar residues in the O-repeat as well as on the arrangement of the monosaccharides and the mode of linkages between them.
Lipopolysaccharide, LPS, structure, strain, Pseudomonas, chain, group, antibodies, antibody, epitope, monoclonal, monoclonal antibodies, monoclonal antibody, epitopes, O-polysaccharide, serogroup, immunochemical, O-chain, pathogen, pathogens, pathovar, Pseudomonas syringae, classification, diversity, serotype-specific
Publisher: Kluwer Academic Publishers, The Netherlands
Correspondence: knirel@ioc.ac.ru
Editors: Rudolph K, Burr TJ, Mansfield JW, Stead DE, Vivian A, von Kietzell J
Institutions: Institute of Medical Technology, University of Tampere, Tampere, Finland, N.D. Zelinsy Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
- Article ID: 1074
Ovod V, Knirel YA, Samson R, Krohn K "Immunochemical characterization and taxonomic evaluation of the O polysaccharides of the lipopolysaccharides of Pseudomonas syringae serogroup O1 strains" -
Journal of Bacteriology 181(22) (1999) 6937-6947
The O polysaccharide (OPS) of the lipopolysaccharide (LPS) of Pseudomonas syringae pv. atrofaciens IMV 7836 and some other strains that are classified in serogroup O1 was shown to be a novel linear α-D-rhamnan with the tetrasaccharide O repeat →3)-α-D-Rhap-(1→3)-α-D-Rhap-(1→2)-α-D-Rhap-(1→2)-α-D-Rhap-(1→ (chemotype 1A). The same α-D-rhamnan serves as the backbone in branched OPSs with lateral (α1→3)-linked D-Rhap, (β1→4)-linked D-GlcpNAc, and (α1→4)-linked D-Fucf residues (chemotypes 1B, 1C, and 1D, respectively). Strains of chemotype 1C demonstrated variations resulting in a decrease of the degree of substitution of the backbone 1A with the lateral D-GlcNAc residue (chemotype 1C-1A), which may be described as branched regular <→ branched irregular → linear OPS structure alterations (1C <→ 1C-1A → 1A). Based on serological data, chemotype 1D was suggested to undergo a 1D <→ 1D-1A alteration, whereas chemotype 1B showed no alteration. A number of OPS backbone-specific monoclonal antibodies (MAbs), Ps(1-2)a, Ps(1-2)a(1), Ps1a, Ps1a(1), and Ps1a(2), as well as MAbs Ps1b, Ps1c, Ps1c(1), Ps1d, Ps(1-2)d, and Ps(1-2)d(1) specific to epitopes related to the lateral sugar substituents of the OPSs, were produced against P. syringae serogroup O1 strains. By using MAbs, some specific epitopes were inferred, serogroup O1 strains were serotyped in more detail, and thus, the serological classification scheme of P. syringae was improved. Screening with MAbs of about 800 strains representing all 56 known P. syringae pathovars showed that the strains classified in serogroup O1 were found among 15 pathovars and the strains with the linear OPSs of chemotype 1A were found among 9 of the 15 pathovars. A possible role for the LPS of P. syringae and related pseudomonads as a phylogenetic marker is discussed.
Lipopolysaccharide, lipopolysaccharides, LPS, strain, structural, characterization, polysaccharide, polysaccharides, Pseudomonas, antibody, epitope, monoclonal, monoclonal antibody, O-polysaccharide, O polysaccharide, bacteria, serogroup, evaluation, backbone, immunochemical, Pseudomonas syringae, classification, linear, taxonomic, D-rhamnose
NCBI PubMed ID: 10559159Journal NLM ID: 2985120RPublisher: American Society for Microbiology
Correspondence: Ltvlov@uta.fi
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Medical Technology, University of Tampere, Tampere, Finland, Pathologie Vegetale, Institut National de la Recherche Agronomique, Beaucouze Cedex, France
Methods: NMR
- Article ID: 1452
Corsaro MM, De Castro C, Molinaro A, Parrilli M "Structure of lipopolysaccharides from phytopathogenic Gram-negative bacteria" -
Book: Recent Research Developments in Phytochemistry (2001) Vol. 5, 119-138
This review collects the structural data of lipopolysaccharide components arising from all phytopathogenic bacteria so far investigated. The structural approaches and the main biological role of these macromolecules are also reported.
Lipopolysaccharide, lipopolysaccharides, structure, core, lipid A, O-polysaccharide, gram negative bacteria
WWW link: https://books.google.ru/books/about/Recent_Research_Developments_in_Phytoche.html?id=5CJacgAACAAJ&redir_esc=yPublisher: Research Signpost, Trivandrum, India
Editors: Pandalai SG
Institutions: Dipartimento di Chimica Organica e Biochimica, Complesso Universitario Monte S.Angelo Via Cintia, 4, 80126 Napoli, Italy
- Article ID: 1465
Knirel YA, Zdorovenko GM "Structures of O-polysaccharide chains of lipopolysaccharides as the basis for classification of Pseudomonas syringae and related strains" -
Book: Pseudomonas Syringae Pathovars and Related Pathogens (series: Developments in Plant Pathology) (1997) 475-480
The O-polysaccharides of various serogroups of P. syringae were found to have similar structures with the main chain of a rhamnan which may carry a monosaccharide side chain of D-rhamnose, D-fucose, 2-acetamido-2-deoxy-D-glucose or 3-acetamido-3,6-dideoxy-D-galactose. The relationship between the serological specificity and the host-plant specificity of P. syringae and the structures of the O-polysaccharides is discussed.
Lipopolysaccharide, structure, O-antigen, O-polysaccharide, serological specificity, Pseudomonas syringae, Serogrouping, Host-plant specificity
Publication DOI: 10.1007/978-94-011-5472-7_85Publisher: Springer Netherlands
Editors: Rudolph K, Burr TJ, Mansfield JW, Stead D, Vivian A, von Kietzell J
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Leninsky Pr. 47, Moscow B-334, Russia, D.K. Zabolotny Institute of Microbiology and Virology, Zabolotnogo 154, Kiev-143, Ukraine
- Article ID: 1777
Knirel YA, Kochetkov NK "The structure of lipopolysaccharides of gram-negative bacteria. III. The structure of O-antigens: A review" -
Biochemistry (Moscow) 59(12) (1994) 1325-1383
This review summarizes data on the composition and structure of the O-antigens, the polysaccharide chains of the outer-membrane lipopolysaccharides (LPS) of Gram-negative bacteria defining the immunospecificity of these microbial cells. Special reference is given to some structural features of the O-antigens, such as the presence of unique monosaccharides and noncarbohydrate components, masked regularity, and the occurrence in one microorganism of LPS with structurally different polysaccharide chains. Antigenic relationships between microorganisms belonging to different taxonomic groups are discussed.
structure, O-antigen, chemical composition, bacterial lipopolysaccharides, Salmonella livingstone C1
NCBI PubMed ID: 7533007Journal NLM ID: 0376536Publisher: Nauka/Interperiodica
Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
- Article ID: 1854
Knirel YA, Zdorovenko GM, Yakovleva LM, Shashkov AS, Solyanik LP, Zakharova IY "Antigenic polysaccharides of bacteria. 28. Structure of O-specific chain of lipopolysaccharide of Pseudomonas syringae pv. atrofaciens K-1025 and Pseudomonas holci 90a (serogroup II)" -
Bioorganicheskaya Khimia = Bioorganic Chemistry [Russian] 14(2) (1988) 166-171
Lipopolysaccharides of serologically related strains of Pseudomonas syringae pv. atrofaciens K-1025 and Pseudomonas holci 90a possess the identical O-specific polysaccharide chains, representing a homopolymer of D-rhamnose. On the basis of methylation, partial and complete Smith degradation, and analysis by 1H- and 13C-NMR-spectroscopy, it was concluded that the repeating unit of the polysaccharide is a branched pentasaccharide of the following structure: (formula; see text)
NCBI PubMed ID: 2454627Journal NLM ID: 7804941Publisher: Moskva: Nauka
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the USSR, Moscow, Russia
Methods: 13C NMR, 1H NMR, methylation, sugar analysis, Smith degradation
- Article ID: 2188
Vinogradov EV, Shashkov AS, Knirel YA, Zdorovenko GM, Solyanik LP, Gvozdyak RI "Somatic antigens of pseudomonads: structure of the O-specific polysaccharide chain of Pseudomonas syringae pv. syringae (cerasi) 435 lipopolysaccharide" -
Carbohydrate Research 212 (1991) 295-299
No abstract available
NCBI PubMed ID: 1720346Publication DOI: 10.1016/0008-6215(91)84069-qJournal NLM ID: 0043535Publisher: Elsevier
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the U.S.S.R., Moscow
Methods: 13C NMR, methylation, GLC-MS, sugar analysis, GLC, Smith degradation, GPC
- Article ID: 2234
Lipkind GM, Shashkov AS, Nifant'ev NE, Kochetkov NK "Computer-assisted analysis of the structure of regular branched polysaccharides containing 2,3-disubstituted rhamnopyranose and mannopyranose residues on the basis of 13C NMR" -
Carbohydrate Research 237 (1992) 11-22
A computer-assisted approach to the analysis of the structure of branched polysaccharides that contain 2,3-di-O-glycosylated α-rhamnopyranose and α-mannopyranose residues is based on evaluation of the 13C NMR spectra, using glycosylation effects and their deviations from additivity (delta delta values) at the branch points. This approach, in combination with monosaccharide and methylation analysis data, has been verified on a series of bacterial polysaccharides of known structure
NCBI PubMed ID: 1284111Publication DOI: 10.1016/S0008-6215(92)84229-LJournal NLM ID: 0043535Publisher: Elsevier
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow
Methods: 13C NMR
- Article ID: 2789
Ovod V, Ashorn P, Yakovleva L, Krohn K "Classification of Pseudomonas syringae with monoclonal antibodies against the core and O-side chains of the lipopolysaccharide" -
Phytopathology 85 (1995) 226-232
Journal NLM ID: 9427222Publisher: American Phytopathological Society
- Article ID: 3313
Zdorovenko GM, Zdorovenko EL, Varbanets LD "Composition, structure, and biological properties of lipopolysaccharides from different strains of Pseudomonas syringae pv. atrofaciens" -
Mikrobiologiia = Microbiology [Russian] 76(6) (2007) 683-697
The composition, structure, and certain biological properties of lipopolysaccharides (LPS) isolated from six strains of bacteria Pseudomonas syringae pv.atrofaciens pathogenic for grain-crops (wheat, rye) are presented. The LPS-protein complexes were isolated by a sparing procedure (extraction from microbial cells with a weak salt solution). They reacted with the homologous O sera and contained one to three antigenic determinants. Against the cells of warm-blooded animals (mice, humans) they exhibited the biological activity typical of endotoxins (stimulation of cytokine production, mitogenetic activity, etc.). The LCD of the biovar type strain was highly toxic to mice sensitized with D-galactosamine. The structural components of LPS macromolecules obtained by mild acidic degradation were characterized: lipid A, core oligosaccharide, and O-specific polysaccharide (OPS). Fatty acids 3-HO-C10:0, C12:0, 2-HO-C12:0, 3-HO-C12:0, C16:0, C16:1, C18:0, and C18:1 were identified in lipid A of all the strains, as well as the components of the hydrophilic part: glucosamine (GlcN), ethanolamine (EtN), phosphate, and phosphoethanolamine (EtN-P). In the core LPS, glucose (Glc), rhamnose(Rha), L-glycero-D-manno-heptose (Hep), GlcN, galactosamine (GalN), 2-keto-3-deoxy-D-mannooctonoi acid (KDO), alanine (Ala), and phosphate were present. The O chain of all the strains consisted of repeated elements containing a linear chain of three to four L- (two strains) or D-Rha (four strains) residues supplemented with a single residue of 3-acetamido-3,6-dideoxy-D-galactose (D-Fucp3Nac), N-acetyl-D-glucosamine(D-GlcpNAc), D-fucose (D-Fucf), or D-Rhap (strain-dependent) as a side substituent. In different strains the substitution position for Rha residues in the repeated components of the major rhamnan chain was also different.One strain exhibited a unique type of O-chain heterogeneity. Immunochemical investigation of the LPS antigenic properties revealed the absence of close serological relations between the strains of one pathovar; this finding correlates with the differences in their OPS structure. Resemblance between the investigated strains and other P.syringae strains with similar LPS structures was revealed. The results of LPS analysis indicate the absence of correlation between the OPS structure and the pathovar affiliation of the strains.
Lipopolysaccharide, structure, lipid A, core oligosaccharide, O-specific polysaccharide, biological activity, immunochemistry, Pseudomonas syringae pv.atrofaciens
NCBI PubMed ID: 18297868Journal NLM ID: 0376652Publisher: Moskva: Izdatelstvo Nauka
Correspondence: alz@i.com.ua; evelina@ioc.ac.ru
Institutions: Zabolotnyi Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kiev, Zelinskii Institute of Organic Chemistry, Russian Academy of Sciences, Moscow
Methods: 13C NMR, 1H NMR, GLC-MS, sugar analysis, serological methods
- Article ID: 3968
Zdorovenko GM, Zdorovenko EL "Pseudomonas syringae lipopolysaccharides: Immunochemical characteristics and structure as a basis for strain classification" -
Mikrobiologiia = Microbiology [Russian] 79(1) (2010) 47-57
Lipopolysaccharide (LPS) preparations of 34 Pseudomonas syringae strains of 19 pathovars were prepared by saline extraction from wet cells and purified by repeated ultracentrifugation. The preparations reacted with homologous O-antisera, obtained by rabbit immunization with heat-killed bacterial cells. Through inhibition of homologous reactions between LPS preparations of heterologous strains (enzyme immunoassay, EIA), it was established for the first time that high serological affinity between strains is observed only if their LPS contains O-specific polysaccharide chains (OPS) comprised of completely identical rather than partially similar units. The central linear part of the OPS was found to be serologically inert when shielded with side groups. Data on immunochemical characteristics of the LPS and OPS structure are analyzed in relation to the design of P. syringae classification scheme.
Lipopolysaccharide, structure, O-specific polysaccharide, Pseudomonas syringae, classification, immunochemistry
NCBI PubMed ID: 20411661Publication DOI: 10.1134/S0026261710010078Journal NLM ID: 0376652Publisher: Moskva: Izdatelstvo Nauka
Correspondence: evelina@ioc.ac.ru
Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Zabolotnyi Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, ul. Zabolotnogo 154, Kyiv, 03143 Ukraine
Methods: partial acid hydrolysis, EIA, serological methods, de-N-acetylation/deamination
Expand this compound
Collapse this compound
7. Compound ID: 2979
|
b-D-GlcpNAc-(1-4)-+
|
-3)-a-D-Rhap-(1-3)-a-D-Rhap-(1-2)-a-D-Rhap-(1-2)-a-D-Rhap-(1- |
Show graphically |
Structure type: polymer chemical repeating unit
Trivial name: D-rhamnan
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_1394181,IEDB_141807,IEDB_144827,IEDB_144829,IEDB_145005,IEDB_145006,IEDB_145008,IEDB_145010,IEDB_151531
The structure is contained in the following publication(s):
- Article ID: 1071
Ovod V, Rudolph K, Knirel YA, Krohn K "Immunochemical characterization of O polysaccharides composing the a-D-rhamnose backbone of lipopolysaccharide of Pseudomonas syringae and classification of bacteria into serogroups O1 and O2 with monoclonal antibodies" -
Journal of Bacteriology 178 (1996) 6459-6465
Murine monoclonal antibodies (MAbs) reacting with Pseudomonas syringae lipopolysaccharide (LPS) O polysaccharides (OPS) composed of tetra- and tri-α-D-rhamnose repeats in the backbone [3)D-Rha(α1-3)D-Rha(α1-2)D-Rha(α1-2)D-Rha(α1] and [3)D-Rha(α1-3)D-Rha(α1-2)D-Rha(α1] were generated and used for immunochemical analysis and for serological classification of the bacteria. A total of 195 of 358 P. syringae strains tested representing 21 pathovars were shown to share a common epitope, 1a, and were classified into serogroup O1. All strains with pathovars aptata, glycinea, japonica, phaseolicola, and pisi, most of the strains with pathovars atrofaciens and striafaciens, and half of the strains with pathovar syringae were classified into serotypes O1a', O1b, O1c, and O1d within serogroup O1. Serogroup-specific epitope 1a was inferred to be related to the (α1-2)D-Rha(α1-3) site of the OPS backbone. The serotype-specific epitopes 1b, 1c, 1d, and 1a' were inferred as relating to the immunodominant lateral (α1-3)D-Rha, (β1-4)D-GlcNAc, and (α1-4)D-Fuc substituents and backbone-located site (α1-3)D-Rha(α1-2), respectively, of OPSs that share the common tetra-D-rhamnose repeats in the backbone. A total of 7.3% of the strains studied, all with pathovars morsprunorum and lapsa, were classified as serotypes O2a and O2d within serogroup 02. Serotype-specific epitope 2a was inferred as being related to the backbone-located site D-Rha(α1-3)D-Rha and epitope 2d to the immunodominant lateral (α1-4)D-Fuc residue of OPS consisting of tri-D-rhamnose repeats in the backbone. Epitope 2d alternated with 2a within the same LPS molecule and did not cross-react with epitope 1d. Serotypes O2a and O2d were observed in some strains correlating with the coexpression of the two chemotypes of OPS by the same strain. The serogroup O1-specific MAb Ps1a reacted weakly but definitely with all strains from serogroup 02. We propose serological formulas for serogroups O1 and 02 as well as for individual strains within these serogroups.
Lipopolysaccharide, LPS, characterization, polysaccharide, polysaccharides, Pseudomonas, antibodies, antibody, epitope, monoclonal, monoclonal antibodies, monoclonal antibody, O-polysaccharide, O polysaccharide, bacteria, serogroup, backbone, immunochemical, Pseudomonas syringae, classification, D-rhamnose
NCBI PubMed ID: 8932301Journal NLM ID: 2985120RPublisher: American Society for Microbiology
Correspondence: Ltvlov@uta.fi
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Medical Technology, University of Tampere, Tampere, Finland, Department of Microbiology and Immunology, University of Kiev, Kiev, Ukraine, Institut fur Pflanzenpathologie und Pflanzenschutz der Georg-August-Universitat, Gottingen, Germany
Methods: serological methods
- Article ID: 1073
Ovod V, Knirel Y, Krohn K "Demonstration of the immunochemical diversity of O-chains of lipopolysaccharide of Pseudomonas syringae and inferring of the serogroup- and serotype-specific epitopes with monoclonal antibodies" -
Proceedings of International Conference on Pseudomonas syringae Pathovars and Related Pathogens (5th : 1995 : Berlin, Germany) (1997) Vol. 9, 532-537
Using serogroup- and serotyppe-specific murine monoclonal antibodies (MAbs) to Pseudomonas syringae lipopolysacharide (LPS) O-polysaccharides (OPS) (=O chains) with elucidated primary chemical structure of the O-repeating units, a rather high diversity of the OPS-related epitopes was demonstrated and most of them were inferred. The immunogenic properties of the O-serogroup- and O-serotype-specific epitopes were shown to depend on the nature and the number of sugar residues in the O-repeat as well as on the arrangement of the monosaccharides and the mode of linkages between them.
Lipopolysaccharide, LPS, structure, strain, Pseudomonas, chain, group, antibodies, antibody, epitope, monoclonal, monoclonal antibodies, monoclonal antibody, epitopes, O-polysaccharide, serogroup, immunochemical, O-chain, pathogen, pathogens, pathovar, Pseudomonas syringae, classification, diversity, serotype-specific
Publisher: Kluwer Academic Publishers, The Netherlands
Correspondence: knirel@ioc.ac.ru
Editors: Rudolph K, Burr TJ, Mansfield JW, Stead DE, Vivian A, von Kietzell J
Institutions: Institute of Medical Technology, University of Tampere, Tampere, Finland, N.D. Zelinsy Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
- Article ID: 1320
Zdorovenko GM, Shashkov AS, Zdorovenko EL, Kocharova NA, Yakovleva LM, Knirel YA, Rudolph K "Characterization of the lipopolysaccharide and structure of the O-specific polysaccharide of the bacterium Pseudomonas syringae pv. atrofaciens IMV 948" -
Biochemistry (Moscow) 66(4) (2001) 369-377
Lipopolysaccharide (LPS) was isolated from the phytopathogenic bacterium Pseudomonas syringae pv. atrofaciens IMV 948 by mild extraction of the microbial cells with saline, and the properties, composition, and structure of the LPS were studied. The LPS showed low toxicity in D- galactosamine-sensitized mice and low biological activity in plants. Structural components of LPS--lipid A, core oligosaccharide, and O-specific polysaccharide (OPS)--were obtained by mild acid degradation and characterized. The lipid A contained fatty acids 3-HO-C10:0, C12:0, 2-HO-C12:0, 3-HO-C12:0, C16:0, C16:1, C18:0, and C18:1, as well as components of the hydrophilic moiety: GlcN, ethanolamine, phosphate, and phosphoethanolamine. The LPS core contained components typical of pseudomonads: glucose, rhamnose (Rha), L-glycero-D-manno-heptose, GlcN, GalN, 2-keto-3-deoxy-D-manno-octonic acid, alanine, and phosphate. The OPS consisted of L-Rha and D-GlcNAc in the ratio 4 : 1 and was structurally heterogeneous. The main pentasaccharide repeating unit of the OPS has the following structure: [structure see text]. Immunochemical studies showed that P. syringae pv. atrofaciens IMV 948 is serologically separate from other P. syringae strains, including those that have structurally similar OPS
Lipopolysaccharide, structure, Pseudomonas syringae, composition, immunochemistry, omposition
NCBI PubMed ID: 11403642Journal NLM ID: 0376536Publisher: Nauka/Interperiodica
Correspondence: zdorov@i.kiev.ua
Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kiev, 252143, Ukraine, Institut fur Pflazenpathologie und Pfalnzenschutz, Georg-August-Universitat, Gottingen, 37077, Germany
Methods: NMR-2D, methylation, NMR
- Article ID: 1452
Corsaro MM, De Castro C, Molinaro A, Parrilli M "Structure of lipopolysaccharides from phytopathogenic Gram-negative bacteria" -
Book: Recent Research Developments in Phytochemistry (2001) Vol. 5, 119-138
This review collects the structural data of lipopolysaccharide components arising from all phytopathogenic bacteria so far investigated. The structural approaches and the main biological role of these macromolecules are also reported.
Lipopolysaccharide, lipopolysaccharides, structure, core, lipid A, O-polysaccharide, gram negative bacteria
WWW link: https://books.google.ru/books/about/Recent_Research_Developments_in_Phytoche.html?id=5CJacgAACAAJ&redir_esc=yPublisher: Research Signpost, Trivandrum, India
Editors: Pandalai SG
Institutions: Dipartimento di Chimica Organica e Biochimica, Complesso Universitario Monte S.Angelo Via Cintia, 4, 80126 Napoli, Italy
- Article ID: 1465
Knirel YA, Zdorovenko GM "Structures of O-polysaccharide chains of lipopolysaccharides as the basis for classification of Pseudomonas syringae and related strains" -
Book: Pseudomonas Syringae Pathovars and Related Pathogens (series: Developments in Plant Pathology) (1997) 475-480
The O-polysaccharides of various serogroups of P. syringae were found to have similar structures with the main chain of a rhamnan which may carry a monosaccharide side chain of D-rhamnose, D-fucose, 2-acetamido-2-deoxy-D-glucose or 3-acetamido-3,6-dideoxy-D-galactose. The relationship between the serological specificity and the host-plant specificity of P. syringae and the structures of the O-polysaccharides is discussed.
Lipopolysaccharide, structure, O-antigen, O-polysaccharide, serological specificity, Pseudomonas syringae, Serogrouping, Host-plant specificity
Publication DOI: 10.1007/978-94-011-5472-7_85Publisher: Springer Netherlands
Editors: Rudolph K, Burr TJ, Mansfield JW, Stead D, Vivian A, von Kietzell J
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Leninsky Pr. 47, Moscow B-334, Russia, D.K. Zabolotny Institute of Microbiology and Virology, Zabolotnogo 154, Kiev-143, Ukraine
- Article ID: 1834
Knirel YA, Zdorovenko GM, Dashunin VM, Yakovleva LM, Shashkov AS, Zakharova IY, Gvozdyak RI, Kochetkov NK "Antigenic polysaccharides of bacteria. 15. Structure of the repeating unit of O-specific polysaccharide chain of Pseudomonas wieringae lipopolysaccharide" -
Bioorganicheskaya Khimia = Bioorganic Chemistry [Russian] 12(9) (1986) 1253-1262
No abstract available
Journal NLM ID: 7804941WWW link: http://www.rjbc.ru/arc/12/9/1253-1262.pdfPublisher: Moskva: Nauka
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the USSR, Moscow, Russia
Methods: 13C NMR, 1H NMR
- Article ID: 2789
Ovod V, Ashorn P, Yakovleva L, Krohn K "Classification of Pseudomonas syringae with monoclonal antibodies against the core and O-side chains of the lipopolysaccharide" -
Phytopathology 85 (1995) 226-232
Journal NLM ID: 9427222Publisher: American Phytopathological Society
- Article ID: 5242
Zdorovenko EL, Besarab NV, Shashkov AS, Novik GI, Shirokov AA, Burov AM, Knirel YA "Investigation of O-polysaccharides from bacterial strains of Pseudomonas genus as potential receptors of bacteriophage BIM BV-45." -
International Journal of Biological Macromolecules 118 (2018) 1065-1072
The structure of potential bacteriophage receptors located on cell walls of Gram-negative bacteria deposited at Belarusian collection of microorganisms was investigated. Studies by 1D and 2D 1H and 13C NMR spectroscopy enabled to elucidate the structure of the O-specific polysaccharides (OPS) constituting lipopolysaccharide (LPS) of some Pseudomonas species. The capacity of bacteriophage to adsorb to LPS molecules was tested.
Pseudomonas, NMR spectroscopy, Bacteriophages, electron microscopy, Adsorption, O-polysaccharides (OPS)
Publication DOI: 10.1016/j.ijbiomac.2018.06.165Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: zdorovenkoe@mail.ru
Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Microbiology, National Academy of Sciences of Belarus, 220141 Minsk, Belarus, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 410049, Saratov, Russian Federation
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, acid hydrolysis, mild acid hydrolysis, GC, GPC, analysis of bacteriophage morphology, analysis of the bacteriophages adsorption
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8. Compound ID: 2980
|
a-D-Fucp-(1-4)-+
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-3)-a-D-Rhap-(1-3)-a-D-Rhap-(1-2)-a-D-Rhap-(1-2)-a-D-Rhap-(1- |
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Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115015,IEDB_1394181,IEDB_142489,IEDB_144827,IEDB_145005,IEDB_145006,IEDB_145009,IEDB_145010,IEDB_149135,SB_86
The structure is contained in the following publication(s):
- Article ID: 1071
Ovod V, Rudolph K, Knirel YA, Krohn K "Immunochemical characterization of O polysaccharides composing the a-D-rhamnose backbone of lipopolysaccharide of Pseudomonas syringae and classification of bacteria into serogroups O1 and O2 with monoclonal antibodies" -
Journal of Bacteriology 178 (1996) 6459-6465
Murine monoclonal antibodies (MAbs) reacting with Pseudomonas syringae lipopolysaccharide (LPS) O polysaccharides (OPS) composed of tetra- and tri-α-D-rhamnose repeats in the backbone [3)D-Rha(α1-3)D-Rha(α1-2)D-Rha(α1-2)D-Rha(α1] and [3)D-Rha(α1-3)D-Rha(α1-2)D-Rha(α1] were generated and used for immunochemical analysis and for serological classification of the bacteria. A total of 195 of 358 P. syringae strains tested representing 21 pathovars were shown to share a common epitope, 1a, and were classified into serogroup O1. All strains with pathovars aptata, glycinea, japonica, phaseolicola, and pisi, most of the strains with pathovars atrofaciens and striafaciens, and half of the strains with pathovar syringae were classified into serotypes O1a', O1b, O1c, and O1d within serogroup O1. Serogroup-specific epitope 1a was inferred to be related to the (α1-2)D-Rha(α1-3) site of the OPS backbone. The serotype-specific epitopes 1b, 1c, 1d, and 1a' were inferred as relating to the immunodominant lateral (α1-3)D-Rha, (β1-4)D-GlcNAc, and (α1-4)D-Fuc substituents and backbone-located site (α1-3)D-Rha(α1-2), respectively, of OPSs that share the common tetra-D-rhamnose repeats in the backbone. A total of 7.3% of the strains studied, all with pathovars morsprunorum and lapsa, were classified as serotypes O2a and O2d within serogroup 02. Serotype-specific epitope 2a was inferred as being related to the backbone-located site D-Rha(α1-3)D-Rha and epitope 2d to the immunodominant lateral (α1-4)D-Fuc residue of OPS consisting of tri-D-rhamnose repeats in the backbone. Epitope 2d alternated with 2a within the same LPS molecule and did not cross-react with epitope 1d. Serotypes O2a and O2d were observed in some strains correlating with the coexpression of the two chemotypes of OPS by the same strain. The serogroup O1-specific MAb Ps1a reacted weakly but definitely with all strains from serogroup 02. We propose serological formulas for serogroups O1 and 02 as well as for individual strains within these serogroups.
Lipopolysaccharide, LPS, characterization, polysaccharide, polysaccharides, Pseudomonas, antibodies, antibody, epitope, monoclonal, monoclonal antibodies, monoclonal antibody, O-polysaccharide, O polysaccharide, bacteria, serogroup, backbone, immunochemical, Pseudomonas syringae, classification, D-rhamnose
NCBI PubMed ID: 8932301Journal NLM ID: 2985120RPublisher: American Society for Microbiology
Correspondence: Ltvlov@uta.fi
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Medical Technology, University of Tampere, Tampere, Finland, Department of Microbiology and Immunology, University of Kiev, Kiev, Ukraine, Institut fur Pflanzenpathologie und Pflanzenschutz der Georg-August-Universitat, Gottingen, Germany
Methods: serological methods
- Article ID: 1073
Ovod V, Knirel Y, Krohn K "Demonstration of the immunochemical diversity of O-chains of lipopolysaccharide of Pseudomonas syringae and inferring of the serogroup- and serotype-specific epitopes with monoclonal antibodies" -
Proceedings of International Conference on Pseudomonas syringae Pathovars and Related Pathogens (5th : 1995 : Berlin, Germany) (1997) Vol. 9, 532-537
Using serogroup- and serotyppe-specific murine monoclonal antibodies (MAbs) to Pseudomonas syringae lipopolysacharide (LPS) O-polysaccharides (OPS) (=O chains) with elucidated primary chemical structure of the O-repeating units, a rather high diversity of the OPS-related epitopes was demonstrated and most of them were inferred. The immunogenic properties of the O-serogroup- and O-serotype-specific epitopes were shown to depend on the nature and the number of sugar residues in the O-repeat as well as on the arrangement of the monosaccharides and the mode of linkages between them.
Lipopolysaccharide, LPS, structure, strain, Pseudomonas, chain, group, antibodies, antibody, epitope, monoclonal, monoclonal antibodies, monoclonal antibody, epitopes, O-polysaccharide, serogroup, immunochemical, O-chain, pathogen, pathogens, pathovar, Pseudomonas syringae, classification, diversity, serotype-specific
Publisher: Kluwer Academic Publishers, The Netherlands
Correspondence: knirel@ioc.ac.ru
Editors: Rudolph K, Burr TJ, Mansfield JW, Stead DE, Vivian A, von Kietzell J
Institutions: Institute of Medical Technology, University of Tampere, Tampere, Finland, N.D. Zelinsy Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
- Article ID: 3313
Zdorovenko GM, Zdorovenko EL, Varbanets LD "Composition, structure, and biological properties of lipopolysaccharides from different strains of Pseudomonas syringae pv. atrofaciens" -
Mikrobiologiia = Microbiology [Russian] 76(6) (2007) 683-697
The composition, structure, and certain biological properties of lipopolysaccharides (LPS) isolated from six strains of bacteria Pseudomonas syringae pv.atrofaciens pathogenic for grain-crops (wheat, rye) are presented. The LPS-protein complexes were isolated by a sparing procedure (extraction from microbial cells with a weak salt solution). They reacted with the homologous O sera and contained one to three antigenic determinants. Against the cells of warm-blooded animals (mice, humans) they exhibited the biological activity typical of endotoxins (stimulation of cytokine production, mitogenetic activity, etc.). The LCD of the biovar type strain was highly toxic to mice sensitized with D-galactosamine. The structural components of LPS macromolecules obtained by mild acidic degradation were characterized: lipid A, core oligosaccharide, and O-specific polysaccharide (OPS). Fatty acids 3-HO-C10:0, C12:0, 2-HO-C12:0, 3-HO-C12:0, C16:0, C16:1, C18:0, and C18:1 were identified in lipid A of all the strains, as well as the components of the hydrophilic part: glucosamine (GlcN), ethanolamine (EtN), phosphate, and phosphoethanolamine (EtN-P). In the core LPS, glucose (Glc), rhamnose(Rha), L-glycero-D-manno-heptose (Hep), GlcN, galactosamine (GalN), 2-keto-3-deoxy-D-mannooctonoi acid (KDO), alanine (Ala), and phosphate were present. The O chain of all the strains consisted of repeated elements containing a linear chain of three to four L- (two strains) or D-Rha (four strains) residues supplemented with a single residue of 3-acetamido-3,6-dideoxy-D-galactose (D-Fucp3Nac), N-acetyl-D-glucosamine(D-GlcpNAc), D-fucose (D-Fucf), or D-Rhap (strain-dependent) as a side substituent. In different strains the substitution position for Rha residues in the repeated components of the major rhamnan chain was also different.One strain exhibited a unique type of O-chain heterogeneity. Immunochemical investigation of the LPS antigenic properties revealed the absence of close serological relations between the strains of one pathovar; this finding correlates with the differences in their OPS structure. Resemblance between the investigated strains and other P.syringae strains with similar LPS structures was revealed. The results of LPS analysis indicate the absence of correlation between the OPS structure and the pathovar affiliation of the strains.
Lipopolysaccharide, structure, lipid A, core oligosaccharide, O-specific polysaccharide, biological activity, immunochemistry, Pseudomonas syringae pv.atrofaciens
NCBI PubMed ID: 18297868Journal NLM ID: 0376652Publisher: Moskva: Izdatelstvo Nauka
Correspondence: alz@i.com.ua; evelina@ioc.ac.ru
Institutions: Zabolotnyi Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kiev, Zelinskii Institute of Organic Chemistry, Russian Academy of Sciences, Moscow
Methods: 13C NMR, 1H NMR, GLC-MS, sugar analysis, serological methods
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9. Compound ID: 3003
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b-D-GlcpN3Ac-(1-4)-+
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-3)-a-D-Rhap-(1-3)-a-D-Rhap-(1-2)-a-D-Rhap-(1-2)-a-D-Rhap-(1- |
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Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_137340,IEDB_1394181,IEDB_141807,IEDB_144827,IEDB_145005,IEDB_145006,IEDB_145010,IEDB_151531
The structure is contained in the following publication(s):
- Article ID: 1074
Ovod V, Knirel YA, Samson R, Krohn K "Immunochemical characterization and taxonomic evaluation of the O polysaccharides of the lipopolysaccharides of Pseudomonas syringae serogroup O1 strains" -
Journal of Bacteriology 181(22) (1999) 6937-6947
The O polysaccharide (OPS) of the lipopolysaccharide (LPS) of Pseudomonas syringae pv. atrofaciens IMV 7836 and some other strains that are classified in serogroup O1 was shown to be a novel linear α-D-rhamnan with the tetrasaccharide O repeat →3)-α-D-Rhap-(1→3)-α-D-Rhap-(1→2)-α-D-Rhap-(1→2)-α-D-Rhap-(1→ (chemotype 1A). The same α-D-rhamnan serves as the backbone in branched OPSs with lateral (α1→3)-linked D-Rhap, (β1→4)-linked D-GlcpNAc, and (α1→4)-linked D-Fucf residues (chemotypes 1B, 1C, and 1D, respectively). Strains of chemotype 1C demonstrated variations resulting in a decrease of the degree of substitution of the backbone 1A with the lateral D-GlcNAc residue (chemotype 1C-1A), which may be described as branched regular <→ branched irregular → linear OPS structure alterations (1C <→ 1C-1A → 1A). Based on serological data, chemotype 1D was suggested to undergo a 1D <→ 1D-1A alteration, whereas chemotype 1B showed no alteration. A number of OPS backbone-specific monoclonal antibodies (MAbs), Ps(1-2)a, Ps(1-2)a(1), Ps1a, Ps1a(1), and Ps1a(2), as well as MAbs Ps1b, Ps1c, Ps1c(1), Ps1d, Ps(1-2)d, and Ps(1-2)d(1) specific to epitopes related to the lateral sugar substituents of the OPSs, were produced against P. syringae serogroup O1 strains. By using MAbs, some specific epitopes were inferred, serogroup O1 strains were serotyped in more detail, and thus, the serological classification scheme of P. syringae was improved. Screening with MAbs of about 800 strains representing all 56 known P. syringae pathovars showed that the strains classified in serogroup O1 were found among 15 pathovars and the strains with the linear OPSs of chemotype 1A were found among 9 of the 15 pathovars. A possible role for the LPS of P. syringae and related pseudomonads as a phylogenetic marker is discussed.
Lipopolysaccharide, lipopolysaccharides, LPS, strain, structural, characterization, polysaccharide, polysaccharides, Pseudomonas, antibody, epitope, monoclonal, monoclonal antibody, O-polysaccharide, O polysaccharide, bacteria, serogroup, evaluation, backbone, immunochemical, Pseudomonas syringae, classification, linear, taxonomic, D-rhamnose
NCBI PubMed ID: 10559159Journal NLM ID: 2985120RPublisher: American Society for Microbiology
Correspondence: Ltvlov@uta.fi
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Medical Technology, University of Tampere, Tampere, Finland, Pathologie Vegetale, Institut National de la Recherche Agronomique, Beaucouze Cedex, France
Methods: NMR
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10. Compound ID: 3004
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a-D-Fucf-(1-4)-+
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-3)-a-D-Rhap-(1-3)-a-D-Rhap-(1-2)-a-D-Rhap-(1-2)-a-D-Rhap-(1- |
Show graphically |
Structure type: polymer chemical repeating unit
Trivial name: D-rhamnan
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_1394181,IEDB_142489,IEDB_144827,IEDB_145005,IEDB_145006,IEDB_145010,SB_86
The structure is contained in the following publication(s):
- Article ID: 1074
Ovod V, Knirel YA, Samson R, Krohn K "Immunochemical characterization and taxonomic evaluation of the O polysaccharides of the lipopolysaccharides of Pseudomonas syringae serogroup O1 strains" -
Journal of Bacteriology 181(22) (1999) 6937-6947
The O polysaccharide (OPS) of the lipopolysaccharide (LPS) of Pseudomonas syringae pv. atrofaciens IMV 7836 and some other strains that are classified in serogroup O1 was shown to be a novel linear α-D-rhamnan with the tetrasaccharide O repeat →3)-α-D-Rhap-(1→3)-α-D-Rhap-(1→2)-α-D-Rhap-(1→2)-α-D-Rhap-(1→ (chemotype 1A). The same α-D-rhamnan serves as the backbone in branched OPSs with lateral (α1→3)-linked D-Rhap, (β1→4)-linked D-GlcpNAc, and (α1→4)-linked D-Fucf residues (chemotypes 1B, 1C, and 1D, respectively). Strains of chemotype 1C demonstrated variations resulting in a decrease of the degree of substitution of the backbone 1A with the lateral D-GlcNAc residue (chemotype 1C-1A), which may be described as branched regular <→ branched irregular → linear OPS structure alterations (1C <→ 1C-1A → 1A). Based on serological data, chemotype 1D was suggested to undergo a 1D <→ 1D-1A alteration, whereas chemotype 1B showed no alteration. A number of OPS backbone-specific monoclonal antibodies (MAbs), Ps(1-2)a, Ps(1-2)a(1), Ps1a, Ps1a(1), and Ps1a(2), as well as MAbs Ps1b, Ps1c, Ps1c(1), Ps1d, Ps(1-2)d, and Ps(1-2)d(1) specific to epitopes related to the lateral sugar substituents of the OPSs, were produced against P. syringae serogroup O1 strains. By using MAbs, some specific epitopes were inferred, serogroup O1 strains were serotyped in more detail, and thus, the serological classification scheme of P. syringae was improved. Screening with MAbs of about 800 strains representing all 56 known P. syringae pathovars showed that the strains classified in serogroup O1 were found among 15 pathovars and the strains with the linear OPSs of chemotype 1A were found among 9 of the 15 pathovars. A possible role for the LPS of P. syringae and related pseudomonads as a phylogenetic marker is discussed.
Lipopolysaccharide, lipopolysaccharides, LPS, strain, structural, characterization, polysaccharide, polysaccharides, Pseudomonas, antibody, epitope, monoclonal, monoclonal antibody, O-polysaccharide, O polysaccharide, bacteria, serogroup, evaluation, backbone, immunochemical, Pseudomonas syringae, classification, linear, taxonomic, D-rhamnose
NCBI PubMed ID: 10559159Journal NLM ID: 2985120RPublisher: American Society for Microbiology
Correspondence: Ltvlov@uta.fi
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Medical Technology, University of Tampere, Tampere, Finland, Pathologie Vegetale, Institut National de la Recherche Agronomique, Beaucouze Cedex, France
Methods: NMR
- Article ID: 1313
Zdorovenko EL, Ovod V, Zatonsky GV, Shashkov AS, Kocharova NA, Knirel YA "Location of the O-methyl groups in the O polysaccharide of Pseudomonas syringae pv. phaseolicola" -
Carbohydrate Research 330(4) (2001) 505-510
The O-methylation pattern of the O polysaccharide (OPS) of the lipopolysaccharide of Pseudomonas syringae pv. phaseolicola GSPB 1552 was revealed by methylation (CD3I) analysis, Smith degradation, and NMR spectroscopy. Together with the major O repeats consisting of D-rhamnopyranose (D-Rhap) and D-fucofuranose (D-Fucf), there are minor repeats (approximately 30%) containing 3-O-methyl-D-rhamnose (D-acofriose), which is 2-substituted in the interior repeats and occupies the terminal non-reducing end of the OPS. It was suggested that the methylated O repeats are linked to each other nearby the non-reducing end of the OPS and that the 'biological' O repeat of the OPS has the following structure: [molecular structure: see text]
Lipopolysaccharide, Pseudomonas syringae, O-polysaccharides, O-methylation, phytopathogens
NCBI PubMed ID: 11269402Publication DOI: 10.1016/S0008-6215(01)00005-2Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: knirel@ioc.ac.ru
Institutions: Institute of Medical Technology, University of Tampere, Tampere, Finland, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Institute of Medical Technology, University of Tampere, Tampere, Finalnd
Methods: NMR-2D, methylation, NMR, Smith degradation
- Article ID: 1317
Zdorovenko GM, Solyanik LP, Zakharova IY, Gubanova NY, Iakovleva LM, Gvozdyak RI "Characterization of lipid A of lipopolysaccharides of Pseudomonas syringae strains" -
Mikrobiologichnyi Zhurnal = Microbiological Journal [Ukrainian] 57(5) (1995) 30-39
Component composition of lipid A of Pseudomonas syringae pv. syringae 218 and P-55, pv. syringae (holci) 8299, pv. phaseolicola 120a, pv. atrofaciens 2399 has been studied. The lipid A composition of all the strains studied included 3-hydroxydecanoic fatty acid (3-OH-C10:0), 2-hydroxydodecanoic (2-OH-C12:0), 3-hydroxydodecanoic (3-OH-C12:0), dodecanoic (C12:0), hexadecanoic (C16:0), octadecanoic (C18:0), hexadecenic (C16:1), octadecenic (C18:1) fatty acids. The carbohydrate part of the lipid A macromolecule of all strains after acid hydrolysis contains ethanolamine, phosphoethanolamine, glucosamine.
lipid A, Pseudomonas syringae, component composision
Journal NLM ID: 7910045Publisher: Kyiv: Naukova Dumka
Correspondence: zdorov@i.kiev.ua
Institutions: Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kiev, Ukraine
- Article ID: 1452
Corsaro MM, De Castro C, Molinaro A, Parrilli M "Structure of lipopolysaccharides from phytopathogenic Gram-negative bacteria" -
Book: Recent Research Developments in Phytochemistry (2001) Vol. 5, 119-138
This review collects the structural data of lipopolysaccharide components arising from all phytopathogenic bacteria so far investigated. The structural approaches and the main biological role of these macromolecules are also reported.
Lipopolysaccharide, lipopolysaccharides, structure, core, lipid A, O-polysaccharide, gram negative bacteria
WWW link: https://books.google.ru/books/about/Recent_Research_Developments_in_Phytoche.html?id=5CJacgAACAAJ&redir_esc=yPublisher: Research Signpost, Trivandrum, India
Editors: Pandalai SG
Institutions: Dipartimento di Chimica Organica e Biochimica, Complesso Universitario Monte S.Angelo Via Cintia, 4, 80126 Napoli, Italy
- Article ID: 1465
Knirel YA, Zdorovenko GM "Structures of O-polysaccharide chains of lipopolysaccharides as the basis for classification of Pseudomonas syringae and related strains" -
Book: Pseudomonas Syringae Pathovars and Related Pathogens (series: Developments in Plant Pathology) (1997) 475-480
The O-polysaccharides of various serogroups of P. syringae were found to have similar structures with the main chain of a rhamnan which may carry a monosaccharide side chain of D-rhamnose, D-fucose, 2-acetamido-2-deoxy-D-glucose or 3-acetamido-3,6-dideoxy-D-galactose. The relationship between the serological specificity and the host-plant specificity of P. syringae and the structures of the O-polysaccharides is discussed.
Lipopolysaccharide, structure, O-antigen, O-polysaccharide, serological specificity, Pseudomonas syringae, Serogrouping, Host-plant specificity
Publication DOI: 10.1007/978-94-011-5472-7_85Publisher: Springer Netherlands
Editors: Rudolph K, Burr TJ, Mansfield JW, Stead D, Vivian A, von Kietzell J
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Leninsky Pr. 47, Moscow B-334, Russia, D.K. Zabolotny Institute of Microbiology and Virology, Zabolotnogo 154, Kiev-143, Ukraine
- Article ID: 1777
Knirel YA, Kochetkov NK "The structure of lipopolysaccharides of gram-negative bacteria. III. The structure of O-antigens: A review" -
Biochemistry (Moscow) 59(12) (1994) 1325-1383
This review summarizes data on the composition and structure of the O-antigens, the polysaccharide chains of the outer-membrane lipopolysaccharides (LPS) of Gram-negative bacteria defining the immunospecificity of these microbial cells. Special reference is given to some structural features of the O-antigens, such as the presence of unique monosaccharides and noncarbohydrate components, masked regularity, and the occurrence in one microorganism of LPS with structurally different polysaccharide chains. Antigenic relationships between microorganisms belonging to different taxonomic groups are discussed.
structure, O-antigen, chemical composition, bacterial lipopolysaccharides, Salmonella livingstone C1
NCBI PubMed ID: 7533007Journal NLM ID: 0376536Publisher: Nauka/Interperiodica
Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
- Article ID: 1853
Knirel YA, Zdorovenko GM, Shashkov AS, Gubanova NY, Yakovleva LM, Gvozdyak RI "Antigenic polysaccharides of bacteria. 27. Structure of the O-specific polysaccharide chain of lipopolysaccharides from Pseudomonas syringae pvs atrofaciens 2399, phaseolicola 120a and Pseudomonas holci 8299, belonging to serogroup VI" -
Bioorganicheskaya Khimia = Bioorganic Chemistry [Russian] 14(1) (1988) 92-99
Lipopolysaccharides from Pseudomonas syringae pvs atrofaciens 2399. phaseolicola 120a and Pseudomonas holci 8299, belonging to serogroup VI. possess an identical polysaccharide chain composed of D-rhamnose and D-fucose. On the hasis of methylation, partial acid hydrolysis, 1H- and 13C-NMR data, it was concluded that the backbone of the polysaccharide represents D-rhamnan built up of tetrasaccharide repeating units and α-D-fucofuranose residues are attached to the backbone as the monosaccharide branches. The following structure of the repeating unit is established: (Formula: see text).
NCBI PubMed ID: 2454626Journal NLM ID: 7804941WWW link: http://www.rjbc.ru/arc/14/1/0092-0099.pdfPublisher: Moskva: Nauka
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the USSR, Moscow, Russia
Methods: 13C NMR, 1H NMR
- Article ID: 2789
Ovod V, Ashorn P, Yakovleva L, Krohn K "Classification of Pseudomonas syringae with monoclonal antibodies against the core and O-side chains of the lipopolysaccharide" -
Phytopathology 85 (1995) 226-232
Journal NLM ID: 9427222Publisher: American Phytopathological Society
- Article ID: 3968
Zdorovenko GM, Zdorovenko EL "Pseudomonas syringae lipopolysaccharides: Immunochemical characteristics and structure as a basis for strain classification" -
Mikrobiologiia = Microbiology [Russian] 79(1) (2010) 47-57
Lipopolysaccharide (LPS) preparations of 34 Pseudomonas syringae strains of 19 pathovars were prepared by saline extraction from wet cells and purified by repeated ultracentrifugation. The preparations reacted with homologous O-antisera, obtained by rabbit immunization with heat-killed bacterial cells. Through inhibition of homologous reactions between LPS preparations of heterologous strains (enzyme immunoassay, EIA), it was established for the first time that high serological affinity between strains is observed only if their LPS contains O-specific polysaccharide chains (OPS) comprised of completely identical rather than partially similar units. The central linear part of the OPS was found to be serologically inert when shielded with side groups. Data on immunochemical characteristics of the LPS and OPS structure are analyzed in relation to the design of P. syringae classification scheme.
Lipopolysaccharide, structure, O-specific polysaccharide, Pseudomonas syringae, classification, immunochemistry
NCBI PubMed ID: 20411661Publication DOI: 10.1134/S0026261710010078Journal NLM ID: 0376652Publisher: Moskva: Izdatelstvo Nauka
Correspondence: evelina@ioc.ac.ru
Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Zabolotnyi Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, ul. Zabolotnogo 154, Kyiv, 03143 Ukraine
Methods: partial acid hydrolysis, EIA, serological methods, de-N-acetylation/deamination
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11. Compound ID: 3202
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a-D-Fucp3NAc-(1-2)-+
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a-D-Fucp3NAc-(1-2)-+ |
| |
-3)-a-D-Rhap-(1-3)-a-D-Rhap-(1-2)-a-D-Rhap-(1-2)-a-D-Rhap-(1- |
Show graphically |
Structure type: polymer biological repeating unit
; n=17
Contained glycoepitopes: IEDB_1394181,IEDB_144827,IEDB_145005,IEDB_145006,IEDB_145010
The structure is contained in the following publication(s):
- Article ID: 1173
Schafer C, Müller N, Christian R, Graninger M, Wugeditsch T, Scheberl A, Messner P "Complete glycan structure of the S-layer glycoprotein of Aneurinibacillus thermoaerophilus GS4-97" -
Glycobiology 9(4) (1999) 407-419
Isolate GS4-97 was purified from an extraction juice sample of an Austrian beet sugar factory and affiliated to the newly described species Aneurinibacillus thermoaerophilus. It is closely related to the type strain of this species, A.thermoaerophilus L420-91(T), and possesses a square surface layer (S-layer) array composed of identical glycoprotein monomers as its outermost cell envelope component. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the purified S-layer showed an apparent molecular mass of approximately 109,000. After thorough proteolytic degradation of this material by pronase E and purification of the reaction mixture by gel permeation, chromatofocusing, and reversed-phase chromatography, a homogeneous glycopeptide fraction was obtained which was subjected to one- and two-dimensional nuclear magnetic resonance spectroscopy. The combined chemical and spectroscopic evidence, together with N-terminal sequencing, suggest the following structure of the O-glycosidically linked S-layer glycan chain of the glycopeptide: This is the first description of a β-D-GalNAc-Thr linkage in glycoproteins.
Structure determination, Aneurinibacillus thermoaerophilus, surface layer (S-layer), prokaryotic glycoprotein, O-glycan
NCBI PubMed ID: 10089215Publication DOI: 10.1093/glycob/9.4.407Journal NLM ID: 9104124Publisher: IRL Press at Oxford University Press
Institutions: Zentrum für Ultrastrukturforshung und Ludwig Boltzmann-Institut fur Molekulare Nanotechnologie Universitat fur Bodenkultur,Wien,Austria
Methods: NMR
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12. Compound ID: 3203
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a-D-Fucp3NAc-(1-2)-+
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a-D-Fucp3NAc-(1-2)-+ |
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Me-3)-{{{-a-D-Rhap-(1-3)-a-D-Rhap-(1-2)-a-D-Rhap-(1-2)-a-D-Rhap-(1-3)-}}}/n=17/-{{{-a-D-Rhap-(1-3)-}}}/n=0-2/-a-D-Rhap-(1-3)-b-D-GalpNAc-(1--/(1->3)Thr peptide/ |
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Structure type: oligomer
Aglycon: (1->3)Thr peptide
Contained glycoepitopes: IEDB_130648,IEDB_137473,IEDB_1394181,IEDB_144827,IEDB_145005,IEDB_145006,IEDB_145010
The structure is contained in the following publication(s):
- Article ID: 1173
Schafer C, Müller N, Christian R, Graninger M, Wugeditsch T, Scheberl A, Messner P "Complete glycan structure of the S-layer glycoprotein of Aneurinibacillus thermoaerophilus GS4-97" -
Glycobiology 9(4) (1999) 407-419
Isolate GS4-97 was purified from an extraction juice sample of an Austrian beet sugar factory and affiliated to the newly described species Aneurinibacillus thermoaerophilus. It is closely related to the type strain of this species, A.thermoaerophilus L420-91(T), and possesses a square surface layer (S-layer) array composed of identical glycoprotein monomers as its outermost cell envelope component. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the purified S-layer showed an apparent molecular mass of approximately 109,000. After thorough proteolytic degradation of this material by pronase E and purification of the reaction mixture by gel permeation, chromatofocusing, and reversed-phase chromatography, a homogeneous glycopeptide fraction was obtained which was subjected to one- and two-dimensional nuclear magnetic resonance spectroscopy. The combined chemical and spectroscopic evidence, together with N-terminal sequencing, suggest the following structure of the O-glycosidically linked S-layer glycan chain of the glycopeptide: This is the first description of a β-D-GalNAc-Thr linkage in glycoproteins.
Structure determination, Aneurinibacillus thermoaerophilus, surface layer (S-layer), prokaryotic glycoprotein, O-glycan
NCBI PubMed ID: 10089215Publication DOI: 10.1093/glycob/9.4.407Journal NLM ID: 9104124Publisher: IRL Press at Oxford University Press
Institutions: Zentrum für Ultrastrukturforshung und Ludwig Boltzmann-Institut fur Molekulare Nanotechnologie Universitat fur Bodenkultur,Wien,Austria
Methods: NMR
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13. Compound ID: 3372
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b-D-GlcNAc-(1-4)-+
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-3)-a-D-Rhap-(1-3)-a-D-Rhap-(1-2)-a-D-Rhap-(1-2)-a-D-Rhap-(1- |
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Structure type: polymer chemical repeating unit
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_1394181,IEDB_141807,IEDB_144827,IEDB_144829,IEDB_145005,IEDB_145006,IEDB_145008,IEDB_145010,IEDB_151531
The structure is contained in the following publication(s):
- Article ID: 1247
Spitali M, Rees N, Wait R, Smith ARW "Structures of lipopolysaccharide side-chains of NCPPB 871, the neopathotype strain of Pseudomonas syringae pv aptata, strain 299A of pv pisi and NCPPB 52, the neopathotype strain of pv phaseolicola, in relation to O-serogroup" -
Phytopathologische Zeitschrift = Journal of Phytopathology 143(11-12) (1995) 663-669
The side-chains of lipopolysaccharides from Pseudomonas syringae pvs. aptata, pisi and phaseolicola contain D-rhamnose. Using 'H- and I3C-nuclear magnetic resonance spectroscopy and methylation analysis, the side-chains of NCPPB 871, the neopathotype strain of pv. aptata, and strain 299 A of pv. pisi race 1 were found to be of identical structure: [formula: see text]. Side-chains from NCPPB 52, the neopathotype strain of pv. phaseolicola, were based on the same backbone structure, but were substituted to the extent of approximately 67+ACU- with branch D-fucose residues: [formula: see text]. Side-chain structures are correlated with a previously proposed ser-ogrouping system.
Lipopolysaccharide, structure, strain, Pseudomonas, side chain, Pseudomonas syringae, O-serotype
Journal NLM ID: 9875585Publisher: Berlin: Parey
Institutions: School of Biological and Chemical Sciences, University of Greenwich, London SE18 6PF, UK, Public Health Laboratory Service, Centre for Applied Microbiology Research, Porton Down, Salisbury SP4 OJG, UK, School of Biological and Chemical Sciences, University of Greenwich, London SE18 6PF, UK, Public Health Laboratory Service, Centre for Applied Microbiology Research, Porton Down, Salisbury SP4 OJG, UK
Methods: 13C NMR, 1H NMR, methylation, serological methods
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14. Compound ID: 3401
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67%a-D-Fucf-(1-4)-+
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-3)-a-D-Rhap-(1-3)-a-D-Rhap-(1-2)-a-D-Rhap-(1-2)-a-D-Rhap-(1- |
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Structure type: polymer chemical repeating unit
Trivial name: side-chain polysaccharide
Contained glycoepitopes: IEDB_1394181,IEDB_142489,IEDB_144827,IEDB_145005,IEDB_145006,IEDB_145010,SB_86
The structure is contained in the following publication(s):
- Article ID: 1247
Spitali M, Rees N, Wait R, Smith ARW "Structures of lipopolysaccharide side-chains of NCPPB 871, the neopathotype strain of Pseudomonas syringae pv aptata, strain 299A of pv pisi and NCPPB 52, the neopathotype strain of pv phaseolicola, in relation to O-serogroup" -
Phytopathologische Zeitschrift = Journal of Phytopathology 143(11-12) (1995) 663-669
The side-chains of lipopolysaccharides from Pseudomonas syringae pvs. aptata, pisi and phaseolicola contain D-rhamnose. Using 'H- and I3C-nuclear magnetic resonance spectroscopy and methylation analysis, the side-chains of NCPPB 871, the neopathotype strain of pv. aptata, and strain 299 A of pv. pisi race 1 were found to be of identical structure: [formula: see text]. Side-chains from NCPPB 52, the neopathotype strain of pv. phaseolicola, were based on the same backbone structure, but were substituted to the extent of approximately 67+ACU- with branch D-fucose residues: [formula: see text]. Side-chain structures are correlated with a previously proposed ser-ogrouping system.
Lipopolysaccharide, structure, strain, Pseudomonas, side chain, Pseudomonas syringae, O-serotype
Journal NLM ID: 9875585Publisher: Berlin: Parey
Institutions: School of Biological and Chemical Sciences, University of Greenwich, London SE18 6PF, UK, Public Health Laboratory Service, Centre for Applied Microbiology Research, Porton Down, Salisbury SP4 OJG, UK, School of Biological and Chemical Sciences, University of Greenwich, London SE18 6PF, UK, Public Health Laboratory Service, Centre for Applied Microbiology Research, Porton Down, Salisbury SP4 OJG, UK
Methods: 13C NMR, 1H NMR, methylation, serological methods
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15. Compound ID: 3455
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a-D-Fucf-(1-4)-+
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-2)-a-D-Rhap3(30%)Me-(1-3)-a-D-Rhap-(1-3)-a-D-Rhap-(1-2)-a-D-Rhap-(1- |
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Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_1394181,IEDB_142489,IEDB_144827,IEDB_145005,IEDB_145006,IEDB_145010,SB_86
The structure is contained in the following publication(s):
- Article ID: 1313
Zdorovenko EL, Ovod V, Zatonsky GV, Shashkov AS, Kocharova NA, Knirel YA "Location of the O-methyl groups in the O polysaccharide of Pseudomonas syringae pv. phaseolicola" -
Carbohydrate Research 330(4) (2001) 505-510
The O-methylation pattern of the O polysaccharide (OPS) of the lipopolysaccharide of Pseudomonas syringae pv. phaseolicola GSPB 1552 was revealed by methylation (CD3I) analysis, Smith degradation, and NMR spectroscopy. Together with the major O repeats consisting of D-rhamnopyranose (D-Rhap) and D-fucofuranose (D-Fucf), there are minor repeats (approximately 30%) containing 3-O-methyl-D-rhamnose (D-acofriose), which is 2-substituted in the interior repeats and occupies the terminal non-reducing end of the OPS. It was suggested that the methylated O repeats are linked to each other nearby the non-reducing end of the OPS and that the 'biological' O repeat of the OPS has the following structure: [molecular structure: see text]
Lipopolysaccharide, Pseudomonas syringae, O-polysaccharides, O-methylation, phytopathogens
NCBI PubMed ID: 11269402Publication DOI: 10.1016/S0008-6215(01)00005-2Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: knirel@ioc.ac.ru
Institutions: Institute of Medical Technology, University of Tampere, Tampere, Finland, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Institute of Medical Technology, University of Tampere, Tampere, Finalnd
Methods: NMR-2D, methylation, NMR, Smith degradation
- Article ID: 3968
Zdorovenko GM, Zdorovenko EL "Pseudomonas syringae lipopolysaccharides: Immunochemical characteristics and structure as a basis for strain classification" -
Mikrobiologiia = Microbiology [Russian] 79(1) (2010) 47-57
Lipopolysaccharide (LPS) preparations of 34 Pseudomonas syringae strains of 19 pathovars were prepared by saline extraction from wet cells and purified by repeated ultracentrifugation. The preparations reacted with homologous O-antisera, obtained by rabbit immunization with heat-killed bacterial cells. Through inhibition of homologous reactions between LPS preparations of heterologous strains (enzyme immunoassay, EIA), it was established for the first time that high serological affinity between strains is observed only if their LPS contains O-specific polysaccharide chains (OPS) comprised of completely identical rather than partially similar units. The central linear part of the OPS was found to be serologically inert when shielded with side groups. Data on immunochemical characteristics of the LPS and OPS structure are analyzed in relation to the design of P. syringae classification scheme.
Lipopolysaccharide, structure, O-specific polysaccharide, Pseudomonas syringae, classification, immunochemistry
NCBI PubMed ID: 20411661Publication DOI: 10.1134/S0026261710010078Journal NLM ID: 0376652Publisher: Moskva: Izdatelstvo Nauka
Correspondence: evelina@ioc.ac.ru
Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Zabolotnyi Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, ul. Zabolotnogo 154, Kyiv, 03143 Ukraine
Methods: partial acid hydrolysis, EIA, serological methods, de-N-acetylation/deamination
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