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Structure type: oligomer
Aglycon: 3-thiopropyl, maleimide-derivatized BSA
Trivial name: tGPI-mucin
Contained glycoepitopes: IEDB_131186,IEDB_134624,IEDB_135818,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_151528,IEDB_167072,IEDB_190606,IEDB_742245,IEDB_742248,IEDB_742250,IEDB_918315,SB_163,SB_165,SB_166,SB_187,SB_195,SB_7,SB_88

• Article ID: 5987
  Ortega-Rodriguez U, Portillo S, Ashmus RA, Duran JA, Schocker NS, Iniguez E, Montoya AL, Zepeda BG, Olivas JJ, Karimi NH, Alonso-Padilla J, Izquierdo L, Pinazo MJ, de Noya BA, Noya O, Maldonado RA, Torrico F, Gascon J, Michael K, Almeida IC "Purification of Glycosylphosphatidylinositol-Anchored Mucins from Trypanosoma cruzi Trypomastigotes and Synthesis of α-Gal-Containing Neoglycoproteins: Application as Biomarkers for Reliable Diagnosis and Early Assessment of Chemotherapeutic Outcomes of Chagas Disease" - Methods in Molecular Biology 1955 (2019) 287-308
  

  Chagas disease (ChD), caused by the protozoan parasite Trypanosoma cruzi, affects millions of people worldwide. Chemotherapy is restricted to two drugs, which are partially effective and may cause severe side effects, leading to cessation of treatment in a significant number of patients. Currently, there are no biomarkers to assess therapeutic efficacy of these drugs in the chronic stage. Moreover, no preventive or therapeutic vaccines are available. In this chapter, we describe the purification of Trypanosoma cruzi trypomastigote-derived glycosylphosphatidylinositol (GPI)-anchored mucins (tGPI-mucins) for their use as antigens for the reliable primary or confirmatory diagnosis and as prognostic biomarkers for early assessment of cure following ChD chemotherapy. We also describe, as an example, the synthesis of a potential tGPI-mucin-derived α-Gal-terminating glycan and its coupling to a carrier protein for use as diagnostic and prognostic biomarker in ChD.

  neoglycoproteins, Trypanosoma cruzi, diagnosis, biomarkers, mucins, Chagas disease, anti-α-Gal antibodies, carbohydrate synthesis, chemiluminescent enzyme-linked immunosorbent assay, chemotherapy

  NCBI PubMed ID: 30868536
  Publication DOI: 10.1007/978-1-4939-9148-8_22
  Journal NLM ID: 9214969
  Publisher: Springer
  Correspondence: icalmeida@utep.edu
  Institutions: Department of Biological Sciences, University of Texas at El Paso, El Paso, TX, USA, Department of Chemistry and Biochemistry, University of Texas at El Paso, El Paso, TX, USA, Barcelona Institute for Global Health (ISGlobal), Hospital Clinic - Universitat de Barcelona, Barcelona, Spain, Barcelona Centre for International Health Research (ISGlobal), Barcelona, Spain, Facultad de Medicina, Instituto de Medicina Tropical, Universidad Central de Venezuela, Caracas, Venezuela, Faculty of Medicine, Universidad Mayor de San Simon, Cochabamba, Bolivia, Fundación CEADES, Cochabamba, Bolivia
  Methods: chemical synthesis, MALDI-TOF MS, enzymatic digestion, extraction, LC-MS, conjugation, immunoreactivity assays, CL-ELISA
  
   
  
  Trypanosoma cruzi
  CSDB ID 8283 (all data & tools)

Show legend Show as text

Structure type: structural motif or average structure ; 126000
Contained glycoepitopes: IEDB_130701,IEDB_131186,IEDB_134624,IEDB_135818,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_137485,IEDB_1394182,IEDB_140529,IEDB_140628,IEDB_141794,IEDB_141806,IEDB_142487,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_152206,IEDB_153201,IEDB_153217,IEDB_153218,IEDB_156493,IEDB_167069,IEDB_167072,IEDB_190606,IEDB_221845,IEDB_241097,IEDB_241101,IEDB_742245,IEDB_742248,IEDB_742250,IEDB_918315,IEDB_983930,IEDB_983931,SB_163,SB_165,SB_166,SB_187,SB_192,SB_195,SB_44,SB_6,SB_67,SB_7,SB_72,SB_88

• Article ID: 6871
  Lee JS, Kwon JS, Won DP, Lee JH, Lee KE, Lee SY, Hong EK "Study of Macrophage Activation and Structural Characteristics of Purified Polysaccharide from the Fruiting Body of Cordyceps militaris" - Journal of Microbiology and Biotechnology 20(7) (2010) 1053-1060
  

  Cordyceps militaris, an entomopathogenic fungus belonging to the class Ascomycetes, has been reported to have beneficial biological activities such as hypoglycemic, anti-inflammatory, antitumor, antimetastatic, hypolipidemic, immunomodulatory, and antioxidant effects. In this study, the crude water-soluble polysaccharide CMP, which was obtained from the fruiting body of C. militaris by hot water extraction and ethanol precipitation, was fractionated by DEAE-cellulose and Sepharose CL-6B column chromatographies. This process resulted in three polysaccharide fractions, termed CMP Fr I, CMP Fr II, and CMP Fr III. Of these fractions, CMP Fr II, with an average molecular mass of 127 kDa, was able to upregulate effectively the phenotypic functions of macrophages such as NO production and cytokine expression. The chemical property of the stimulatory polysaccharide, CMP Fr II, was determined based on its monosaccharide composition, which consisted of glucose (56.4%), galactose (26.4%), and mannose (17.2%). Its structural characteristics were investigated by a combination of chemical and instrumental analyses, including methylation, reductive cleavage, acetylation, Fourier transform infrared spectroscopy (FT- IR), and gas chromatography mass spectrometry (GC- MS). Results indicated that CMP Fr II consisted of the (1 -> 4) or (1 -> 2) linked glucopyranosyl or galactopyranosyl residue with a (1 -> 2) or (1 -> 6) linked mannopyranosyl, glucopyranosyl, or galactopyranosyl residue as a side chain. The configuration of the beta-linkage and random coil conformation of CMP Fr II were confirmed using a Fungi-Fluor kit and Congo red reagent, respectively.

  immunostimulating polysaccharide, macrophage activation, Cordyceps militaris, mushroom-derived hetero-beta-glucan

  NCBI PubMed ID: 20668397
  Publication DOI: 10.4014/jmb.0910.10022
  Journal NLM ID: 9431852
  Publisher: Seoul: Korean Society for Microbiology and Biotechnology
  Correspondence: ekhong@kangwon.ac.kr
  Institutions: Department of Bioengineering and Technology, Kangwon National University, Chuncheon, South Korea
  Methods: gel filtration, GC-MS, ELISA, FTIR, composition analysis, RT-PCR, statistical analysis, methylation analysis, RP-HPLC, TNF-a assay
  
   
  
  Cordyceps militaris
  CSDB ID 41713 (all data & tools)