Found 14 structures.
Displayed structures from 1 to 14
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1. Compound ID: 1660
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a-D-Glcp-(1-2)-b-D-Glcp-(1-2)-+
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a-D-Glcp-(1-3)-+ |
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a-D-GalpNAc-(1-2)-b-D-Manp-(1-4)-a-D-Manp-(1-5)-a-Kdop-(2-6)-b-D-GlcpN-(1-6)-D-GlcN
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Subst-(1-4)-+
Subst = O-antigen |
Show graphically |
Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130648,IEDB_130650,IEDB_130701,IEDB_137340,IEDB_137473,IEDB_137485,IEDB_1391961,IEDB_141584,IEDB_141807,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_152206,IEDB_153219,IEDB_244149,IEDB_885822,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_72
The structure is contained in the following publication(s):
- Article ID: 516
Vinogradov E, Perry MB "Characterisation of the core part of the lipopolysaccharide O-antigen of Francisella novicida (U112)" -
Carbohydrate Research 339(9) (2004) 1643-1648
Francisella novicida (U112), a close relative of the highly virulent bacterium F. tularensis, is known to produce a lipopolysaccharide that is significantly different in biological properties from the LPS of F. tularensis. Here we present the results of the structural analysis of the F. novicida LPS core part, which is found to be similar to that of F. tularensis, differing only by one additional α-Glc residue:where R is an O-chain, linked via a β-bacillosamine (2,4-diamino-2,4,6-trideoxyglucose) residue. The lipid part of F. novicida LPS contains no phosphate substituent and apparently has a free reducing end, a feature also noted in F. tularensis LPS.
LPS, core structure, Francisella, Francisella novicida
NCBI PubMed ID: 15183739Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: evguenii.vinogradov@nrc-cnrc.gc.ca
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada K1A 0R6
Methods: NMR-2D, methylation, NMR, MS
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2. Compound ID: 1661
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a-D-Glcp-(1-2)-b-D-Glcp-(1-2)-+
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a-D-Glcp-(1-3)-+ |
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a-D-GalpNAc-(1-2)-b-D-Manp-(1-4)-a-D-Manp-(1-5)-Kdo |
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Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130648,IEDB_130650,IEDB_130701,IEDB_137473,IEDB_137485,IEDB_1391961,IEDB_141584,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_152206,IEDB_153219,IEDB_244149,IEDB_885822,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_72
The structure is contained in the following publication(s):
- Article ID: 516
Vinogradov E, Perry MB "Characterisation of the core part of the lipopolysaccharide O-antigen of Francisella novicida (U112)" -
Carbohydrate Research 339(9) (2004) 1643-1648
Francisella novicida (U112), a close relative of the highly virulent bacterium F. tularensis, is known to produce a lipopolysaccharide that is significantly different in biological properties from the LPS of F. tularensis. Here we present the results of the structural analysis of the F. novicida LPS core part, which is found to be similar to that of F. tularensis, differing only by one additional α-Glc residue:where R is an O-chain, linked via a β-bacillosamine (2,4-diamino-2,4,6-trideoxyglucose) residue. The lipid part of F. novicida LPS contains no phosphate substituent and apparently has a free reducing end, a feature also noted in F. tularensis LPS.
LPS, core structure, Francisella, Francisella novicida
NCBI PubMed ID: 15183739Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: evguenii.vinogradov@nrc-cnrc.gc.ca
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada K1A 0R6
Methods: NMR-2D, methylation, NMR, MS
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3. Compound ID: 1662
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a-D-Glcp-(1-2)-b-D-Glcp-(1-2)-+
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a-D-GalpNAc-(1-2)-+ |
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a-D-GalpNAcA6NH2-(1-4)-a-D-GalpNAcA6NH2-(1-4)-a-D-GalpNAcA6NH2-(1-3)-b-D-QuipNAc4NAc-(1-4)-b-D-Manp-(1-4)-a-D-Manp-(1-5)-Kdo
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a-D-Glcp-(1-3)-+ |
Show graphically |
Structure type: oligomer
Compound class: core oligosaccharide with O-unit
Contained glycoepitopes: IEDB_130648,IEDB_130650,IEDB_130701,IEDB_137473,IEDB_137485,IEDB_1391961,IEDB_141584,IEDB_142345,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_152206,IEDB_153219,IEDB_244149,IEDB_885822,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_72
The structure is contained in the following publication(s):
- Article ID: 516
Vinogradov E, Perry MB "Characterisation of the core part of the lipopolysaccharide O-antigen of Francisella novicida (U112)" -
Carbohydrate Research 339(9) (2004) 1643-1648
Francisella novicida (U112), a close relative of the highly virulent bacterium F. tularensis, is known to produce a lipopolysaccharide that is significantly different in biological properties from the LPS of F. tularensis. Here we present the results of the structural analysis of the F. novicida LPS core part, which is found to be similar to that of F. tularensis, differing only by one additional α-Glc residue:where R is an O-chain, linked via a β-bacillosamine (2,4-diamino-2,4,6-trideoxyglucose) residue. The lipid part of F. novicida LPS contains no phosphate substituent and apparently has a free reducing end, a feature also noted in F. tularensis LPS.
LPS, core structure, Francisella, Francisella novicida
NCBI PubMed ID: 15183739Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: evguenii.vinogradov@nrc-cnrc.gc.ca
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada K1A 0R6
Methods: NMR-2D, methylation, NMR, MS
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4. Compound ID: 1663
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a-D-Glcp-(1-2)-b-D-Glcp-(1-2)-+
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a-D-GalpNAc-(1-2)-+ |
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a-D-GalpNAcA6NH2-(1-4)-a-D-GalpNAcA6NH2-(1-4)-a-D-GalpNAcA6NH2-(1-3)-a-D-QuipNAc4NAc-(1-4)-a-D-GalpNAcA6NH2-(1-4)-a-D-GalpNAcA6NH2-(1-4)-a-D-GalpNAcA6NH2-(1-3)-b-D-QuipNAc4NAc-(1-4)-b-D-Manp-(1-4)-a-D-Manp-(1-5)-Kdo
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a-D-Glcp-(1-3)-+ |
Show graphically |
Structure type: oligomer
Compound class: core oligosaccharide with two O-units
Contained glycoepitopes: IEDB_130648,IEDB_130650,IEDB_130701,IEDB_137473,IEDB_137485,IEDB_1391961,IEDB_141584,IEDB_142345,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_152206,IEDB_153219,IEDB_244149,IEDB_885822,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_72
The structure is contained in the following publication(s):
- Article ID: 516
Vinogradov E, Perry MB "Characterisation of the core part of the lipopolysaccharide O-antigen of Francisella novicida (U112)" -
Carbohydrate Research 339(9) (2004) 1643-1648
Francisella novicida (U112), a close relative of the highly virulent bacterium F. tularensis, is known to produce a lipopolysaccharide that is significantly different in biological properties from the LPS of F. tularensis. Here we present the results of the structural analysis of the F. novicida LPS core part, which is found to be similar to that of F. tularensis, differing only by one additional α-Glc residue:where R is an O-chain, linked via a β-bacillosamine (2,4-diamino-2,4,6-trideoxyglucose) residue. The lipid part of F. novicida LPS contains no phosphate substituent and apparently has a free reducing end, a feature also noted in F. tularensis LPS.
LPS, core structure, Francisella, Francisella novicida
NCBI PubMed ID: 15183739Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: evguenii.vinogradov@nrc-cnrc.gc.ca
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada K1A 0R6
Methods: NMR-2D, methylation, NMR, MS
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5. Compound ID: 1667
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a-D-Glcp-(1-2)-b-D-Glcp-(1-2)-+
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a-D-GalpNAc-(1-2)-+ |
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b-L-4dthrHexp4enNA-(1-3)-b-D-QuipNAc4NAc-(1-4)-b-D-Manp-(1-4)-a-D-Manp-(1-5)-a-Kdo-(2-6)-b-D-GlcpNAc-(1-6)-D-GlcNAc-ol
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a-D-Glcp-(1-3)-+ |
Show graphically |
Structure type: oligomer
Compound class: LPS
Contained glycoepitopes: IEDB_130648,IEDB_130650,IEDB_130701,IEDB_135813,IEDB_137340,IEDB_137473,IEDB_137485,IEDB_1391961,IEDB_141584,IEDB_141807,IEDB_142345,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_152206,IEDB_153219,IEDB_244149,IEDB_885822,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_72
The structure is contained in the following publication(s):
- Article ID: 516
Vinogradov E, Perry MB "Characterisation of the core part of the lipopolysaccharide O-antigen of Francisella novicida (U112)" -
Carbohydrate Research 339(9) (2004) 1643-1648
Francisella novicida (U112), a close relative of the highly virulent bacterium F. tularensis, is known to produce a lipopolysaccharide that is significantly different in biological properties from the LPS of F. tularensis. Here we present the results of the structural analysis of the F. novicida LPS core part, which is found to be similar to that of F. tularensis, differing only by one additional α-Glc residue:where R is an O-chain, linked via a β-bacillosamine (2,4-diamino-2,4,6-trideoxyglucose) residue. The lipid part of F. novicida LPS contains no phosphate substituent and apparently has a free reducing end, a feature also noted in F. tularensis LPS.
LPS, core structure, Francisella, Francisella novicida
NCBI PubMed ID: 15183739Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: evguenii.vinogradov@nrc-cnrc.gc.ca
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada K1A 0R6
Methods: NMR-2D, methylation, NMR, MS
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6. Compound ID: 7116
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b-D-Glcp-(1-2)-+
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a-D-GalpNAc-(1-2)-+ |
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Subst-(?-3)-b-D-QuipNAc-(1-4)-b-D-Manp-(1-4)-a-D-Manp-(1-5)-a-Kdop-(2-6)-b-D-GlcpN-(1-6)-D-GlcN
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a-D-Glcp-(1-3)-+
Subst = O-antigen |
Show graphically |
Structure type: oligomer
Compound class: LOS
Contained glycoepitopes: IEDB_130648,IEDB_130650,IEDB_130701,IEDB_137340,IEDB_137473,IEDB_137485,IEDB_1391961,IEDB_141584,IEDB_141807,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_152206,IEDB_153219,IEDB_244149,IEDB_885822,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_72
The structure is contained in the following publication(s):
- Article ID: 3230
Kay W, Petersen BO, Duus J, Perry MB, Vinogradov E "Characterization of the lipopolysaccharide and b-glucan of the fish pathogen Francisella victoria" -
FEBS Journal 273(13) (2006) 3002-3013
Lipopolysaccharide (LPS) and b-glucan from Francisella victoria, a fish pathogen and close relative of highly virulent mammal pathogen Francisella tularensis, have been analyzed using chemical and spectroscopy methods. The polysaccharide part of the LPS was found to contain a nonrepetitive sequence of 20 monosaccharides as well as alanine, 3-aminobutyric acid, and a novel branched amino acid, thus confirming F. victoria as a unique species. The structure identified composes the largest oligosaccharide elucidated by NMR so far, and was possible to solve using high field NMR with cold probe technology combined with the latest pulse sequences, including the first application of H2BC sequence to oligosaccharides. The nonphosphorylated lipid A region of the LPS was identical to that of other Francisellae, although one of the lipid A components has not been found in Francisella novicida. The heptoseless core-lipid A region of the LPS contained a linear pentasaccharide fragment identical to the corresponding part of F. tularensis and F. novicida LPSs, differing in side-chain substituents. The linkage region of the O-chain also closely resembled that of other Francisella. LPS preparation contained two characteristic glucans, previously observed as components of LPS preparations from other strains of Francisella: amylose and the unusual b-(1-6)-glucan with (glycerol)2phosphate at the reducing end.
core, lipid A, O-chain, Francisella, Francisella victoria, lipopolysacchride
NCBI PubMed ID: 16759227Publication DOI: 10.1111/j.1742-4658.2006.05311.xJournal NLM ID: 101229646Publisher: Blackwell Publishing
Correspondence: evguenii.vinogradov@nrc.ca
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada, Department of Biochemistry and Microbiology, University of Victoria, BC, Canada, Carlsberg Laboratory, Copenhagen, Denmark
Methods: NMR, ESI-MS, MALDI-MS, composition analysis
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7. Compound ID: 7117
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a-D-Glcp-(1-2)-b-D-Glcp-(1-2)-+
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a-D-GalpNAc-(1-2)-+ |
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Subst-(?-3)-b-D-QuipNAc4NAc-(1-4)-b-D-Manp-(1-4)-a-D-Manp-(1-5)-a-Kdop-(2-6)-b-D-GlcpN-(1-6)-D-GlcN
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a-D-Glcp-(1-3)-+
Subst = O-antigen |
Show graphically |
Structure type: oligomer
Compound class: LOS
Contained glycoepitopes: IEDB_130648,IEDB_130650,IEDB_130701,IEDB_137340,IEDB_137473,IEDB_137485,IEDB_1391961,IEDB_141584,IEDB_141807,IEDB_142345,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_152206,IEDB_153219,IEDB_244149,IEDB_885822,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_72
The structure is contained in the following publication(s):
- Article ID: 3230
Kay W, Petersen BO, Duus J, Perry MB, Vinogradov E "Characterization of the lipopolysaccharide and b-glucan of the fish pathogen Francisella victoria" -
FEBS Journal 273(13) (2006) 3002-3013
Lipopolysaccharide (LPS) and b-glucan from Francisella victoria, a fish pathogen and close relative of highly virulent mammal pathogen Francisella tularensis, have been analyzed using chemical and spectroscopy methods. The polysaccharide part of the LPS was found to contain a nonrepetitive sequence of 20 monosaccharides as well as alanine, 3-aminobutyric acid, and a novel branched amino acid, thus confirming F. victoria as a unique species. The structure identified composes the largest oligosaccharide elucidated by NMR so far, and was possible to solve using high field NMR with cold probe technology combined with the latest pulse sequences, including the first application of H2BC sequence to oligosaccharides. The nonphosphorylated lipid A region of the LPS was identical to that of other Francisellae, although one of the lipid A components has not been found in Francisella novicida. The heptoseless core-lipid A region of the LPS contained a linear pentasaccharide fragment identical to the corresponding part of F. tularensis and F. novicida LPSs, differing in side-chain substituents. The linkage region of the O-chain also closely resembled that of other Francisella. LPS preparation contained two characteristic glucans, previously observed as components of LPS preparations from other strains of Francisella: amylose and the unusual b-(1-6)-glucan with (glycerol)2phosphate at the reducing end.
core, lipid A, O-chain, Francisella, Francisella victoria, lipopolysacchride
NCBI PubMed ID: 16759227Publication DOI: 10.1111/j.1742-4658.2006.05311.xJournal NLM ID: 101229646Publisher: Blackwell Publishing
Correspondence: evguenii.vinogradov@nrc.ca
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada, Department of Biochemistry and Microbiology, University of Victoria, BC, Canada, Carlsberg Laboratory, Copenhagen, Denmark
Methods: NMR, ESI-MS, MALDI-MS, composition analysis
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8. Compound ID: 7597
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a-D-Glcp-(1-2)-b-D-Glcp-(1-2)-+
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a-D-Glcp-(1-3)-+ |
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a-D-GalpNAc-(1-2)-b-D-Manp-(1-4)-a-D-Manp-(1-5)-a-Kdop-(2--/(2->6)lipid A/
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Subst-(1-4)-+
Subst = O-antigen |
Show graphically |
Structure type: oligomer
Aglycon: (2->6)lipid A
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130648,IEDB_130650,IEDB_130701,IEDB_137473,IEDB_137485,IEDB_1391961,IEDB_141584,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_152206,IEDB_153219,IEDB_244149,IEDB_885822,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_72
The structure is contained in the following publication(s):
- Article ID: 3402
Gunn JS, Ernst RK "The structure and function of Francisella lipopolysaccharide" -
Annals of the New York Academy of Sciences 1105 (2007) 202-218
A key factor in the biology of Francisella spp. is lipopolysaccharide (LPS). Francisella LPS has many unique structural properties and poorly activates proinflammatory responses due to its lack of interaction with toll-like receptor 4 (TLR4). The LPS of this organism can be modified by various carbohydrates including glucose, mannose and galactosamine, which affect various aspects of virulence. Spontaneously occurring colony variants of F. tularensis have altered LPS. This altered LPS may account for the novel phenotypes of these variants that include effects on susceptibility to killing by normal human serum, intracellular survival and animal virulence. Mutants devoid of O-antigen (directed mutants in O-antigen biosynthetic genes) show reduced intracellular survival and mouse virulence. Thus, this surface component is important in F. tularensis pathogenesis, and the inability of the LPS to alarm the immune system coupled with its frequent modification/alteration likely aid the success of this pathogen during human infection
Lipopolysaccharide, LPS, Phase variation, O-antigen, lipid A, variant, Francisella, gray variant, LPS modification, LPS structure
NCBI PubMed ID: 17395723Journal NLM ID: 7506858Publisher: New York Academy of Sciences
Correspondence: gunn.43@osu.edu
Institutions: The Center for Microbial Interface Biology, The Ohio State University, Biomedical Research Tower, Rm. 1006, 460 W. 12th Ave., Columbus, OH 43210-1214, USA
Methods: 13C NMR, 1H NMR, NMR-2D, GLC-MS, 31P NMR, GLC, chemical methods, MALDI-TOF MS, NMR-1D, serological methods, genetic methods, CE-ESI-MS
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9. Compound ID: 9086
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b-D-Glcp-(1-2)-+
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a-D-GalpNAc-(1-2)-+ |
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b-D-Quip4N-(1-4)-a-D-GalpNAcA6NH2-(1-4)-a-D-GalpNAcA6NH2-(1-3)-b-D-QuipNAc-(1-4)-b-D-Manp-(1-4)-a-D-Manp-(1-5)-a-Kdo
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a-D-Glcp-(1-3)-+ |
Show graphically |
Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130648,IEDB_130650,IEDB_130701,IEDB_137473,IEDB_137485,IEDB_1391961,IEDB_141584,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_152206,IEDB_153219,IEDB_244149,IEDB_885822,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_72
The structure is contained in the following publication(s):
- Article ID: 3902
Mokrievich AN, Kondakova AN, Valade E, Platonov ME, Vakhrameeva GM, Shaikhutdinova RZ, Mironova RI, Blaha D, Bakhteeva IV, Titareva GM, Kravchenko TB, Kombarova TI, Vidal D, Pavlov VM, Lindner B, Dyatlov IA, Knirel YA "Biological properties and structure of the lipopolysaccharide of a vaccine strain of Francisella tularensis generated by inactivation of a quorum sensing system gene qseC" -
Biochemistry (Moscow) 75(4) (2010) 443-451
A knockout mutant with a deletion in a quorum sensing system gene qseC was generated from the vaccine strain Francisella tularensis 15 by site-directed mutagenesis. The variant with the inactivated gene qseC differed from the parental strain in growth rate on solid nutrient medium but had the same growth dynamics in liquid nutrient medium. The mutation abolished almost completely the resistance of the vaccine strain to normal rabbit serum and its ability to survive in macrophages; in addition, the strain lost the residual virulence. A significant phenotypic alteration was observed in the lipopolysaccharide of F. tularensis. Particularly, the mutant strain synthesized no noticeable amount of the lipopolysaccharide with the high-molecular-mass O-polysaccharide, presumably as a result of impairing biosynthesis of the repeating unit, namely, a loss of the ability to incorporate a formyl group, an N-acyl substituent of 4-amino-4,6-dideoxy-D-glucose.
Lipopolysaccharide, Francisella tularensis, tularemia, quorum sensing, gene qseC, sitedirected mutagenesis
NCBI PubMed ID: 20618133Journal NLM ID: 0376536Publisher: Nauka/Interperiodica
Correspondence: mokrievich@obolensk.org
Institutions: State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, 142279, Russia
Methods: SDS-PAGE, DNA techniques, mild acid hydrolysis, ESI-FTICR-MS, Western blotting, genetic methods
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10. Compound ID: 9087
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b-D-Glcp-(1-2)-+
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a-D-GalpNAc-(1-2)-b-D-Manp-(1-4)-a-D-Manp-(1-5)-a-Kdo
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a-D-Glcp-(1-3)-+ |
Show graphically |
Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130648,IEDB_130650,IEDB_130701,IEDB_137473,IEDB_137485,IEDB_1391961,IEDB_141584,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_152206,IEDB_153219,IEDB_244149,IEDB_885822,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_72
The structure is contained in the following publication(s):
- Article ID: 3902
Mokrievich AN, Kondakova AN, Valade E, Platonov ME, Vakhrameeva GM, Shaikhutdinova RZ, Mironova RI, Blaha D, Bakhteeva IV, Titareva GM, Kravchenko TB, Kombarova TI, Vidal D, Pavlov VM, Lindner B, Dyatlov IA, Knirel YA "Biological properties and structure of the lipopolysaccharide of a vaccine strain of Francisella tularensis generated by inactivation of a quorum sensing system gene qseC" -
Biochemistry (Moscow) 75(4) (2010) 443-451
A knockout mutant with a deletion in a quorum sensing system gene qseC was generated from the vaccine strain Francisella tularensis 15 by site-directed mutagenesis. The variant with the inactivated gene qseC differed from the parental strain in growth rate on solid nutrient medium but had the same growth dynamics in liquid nutrient medium. The mutation abolished almost completely the resistance of the vaccine strain to normal rabbit serum and its ability to survive in macrophages; in addition, the strain lost the residual virulence. A significant phenotypic alteration was observed in the lipopolysaccharide of F. tularensis. Particularly, the mutant strain synthesized no noticeable amount of the lipopolysaccharide with the high-molecular-mass O-polysaccharide, presumably as a result of impairing biosynthesis of the repeating unit, namely, a loss of the ability to incorporate a formyl group, an N-acyl substituent of 4-amino-4,6-dideoxy-D-glucose.
Lipopolysaccharide, Francisella tularensis, tularemia, quorum sensing, gene qseC, sitedirected mutagenesis
NCBI PubMed ID: 20618133Journal NLM ID: 0376536Publisher: Nauka/Interperiodica
Correspondence: mokrievich@obolensk.org
Institutions: State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, 142279, Russia
Methods: SDS-PAGE, DNA techniques, mild acid hydrolysis, ESI-FTICR-MS, Western blotting, genetic methods
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11. Compound ID: 9852
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b-D-Glcp-(1-2)-+
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a-D-GalpNAc-(1-2)-+ |
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Subst-(?-3)-b-D-QuipNAc-(1-4)-b-D-Manp-(1-4)-a-D-Manp-(1-5)-a-Kdop-(2-6)-b-D-GlcpN-(1-6)-D-GlcpN
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a-D-Glcp-(1-3)-+
Subst = O-antigen |
Show graphically |
Structure type: oligomer
Compound class: LPS
Contained glycoepitopes: IEDB_130648,IEDB_130650,IEDB_130701,IEDB_137340,IEDB_137473,IEDB_137485,IEDB_1391961,IEDB_141584,IEDB_141807,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_152206,IEDB_153219,IEDB_244149,IEDB_885822,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_72
The structure is contained in the following publication(s):
- Article ID: 4111
Shilova NV, Navakouski MJ, Huflejt M, Kuehn A, Grunow R, Blixt O, Bovin NV "Changes in the repertoire of natural antibodies caused by immunization with bacterial antigens" -
Biochemistry (Moscow) 76(7) (2011) 862-866
The repertoire of natural anti-glycan antibodies in naive chickens and in chickens immunized with bacteria Burkholderia mallei, Burkholderia pseudomallei, and Francisella tularensis as well as with peptides from an outer membrane protein of B. pseudomallei was studied. A relatively restricted pattern of natural antibodies (first of all IgY against bacterial cell wall peptidoglycan fragments, L-Rha, and core N-acetyllactosamine) shrank and, moreover, the level of detectable antibodies decreased as a result of immunization.
Burkholderia pseudomallei, Francisella tularensis, bacterial polysaccharides, Burkholderia mallei, natural antibodies
NCBI PubMed ID: 21999548Publication DOI: 10.1134/S0006297911070170Journal NLM ID: 0376536Publisher: Nauka/Interperiodica
Correspondence: bovin@carbohydrate.ru
Institutions: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. MiklukhoMaklaya 16/10, 117997 Moscow, Russia
Methods: serological methods, immunization
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12. Compound ID: 11357
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R-3HOSte-(1-2)-+
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a-D-Glcp-(1-2)-b-D-Glcp-(1-2)-+ |
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a-D-Glcp-(1-3)-+ | Pam-(1-3)-R-3HOSte-(1-2)-+ |
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a-D-GalpNAc-(1-2)-b-D-Manp-(1-4)-a-D-Manp-(1-5)-a-Kdop-(2-6)-b-D-GlcpN-(1-6)-a-D-GlcpN-(1--P--1)--a-D-GalpN
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Subst-(1-4)-+ R-3HOSte-(1-3)-+
Subst = O-antigen (ID 29300) |
Show graphically |
Structure type: oligomer
Compound class: LOS
Contained glycoepitopes: IEDB_130648,IEDB_130650,IEDB_130701,IEDB_137340,IEDB_137473,IEDB_137485,IEDB_1391961,IEDB_141181,IEDB_141584,IEDB_141807,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_152206,IEDB_153219,IEDB_244149,IEDB_885822,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_72
The structure is contained in the following publication(s):
- Article ID: 4585
Okan NA, Kasper DL "The atypical lipopolysaccharide of Francisella" -
Carbohydrate Research 378 (2013) 79-83
Bacterial lipopolysaccharides (LPSs) are ubiquitous molecules that are prominent components of the outer membranes of most gram-negative bacteria. Genetic and structural characterizations of Francisella LPS have revealed substantial differences when compared to more commonly studied LPSs of the Enterobacteriaceae. This review discusses both the general characteristics and the unusual features of Francisella LPS.
structure, O-antigen, lipid A, Francisella tularensis, Kdo hydrolase, lipopolysachharide
NCBI PubMed ID: 23916469Publication DOI: 10.1016/j.carres.2013.06.015Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: D.L. Kasper
Institutions: Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA, USA
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13. Compound ID: 11358
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R-3HOSte-(1-2)-+
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b-D-Glcp-(1-2)-+ |
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a-D-Glcp-(1-3)-+ | Pam-(1-3)-R-3HOSte-(1-2)-+ |
| | | |
a-D-GalpNAc-(1-2)-b-D-Manp-(1-4)-a-D-Manp-(1-5)-a-Kdop-(2-6)-b-D-GlcpN-(1-6)-a-D-GlcpN-(1--P--1)--a-D-GalpN
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Subst-(1-4)-+ R-3HOSte-(1-3)-+
Subst = O-antigen (ID 29667) |
Show graphically |
Structure type: oligomer
Compound class: LOS
Contained glycoepitopes: IEDB_130648,IEDB_130650,IEDB_130701,IEDB_137340,IEDB_137473,IEDB_137485,IEDB_1391961,IEDB_141181,IEDB_141584,IEDB_141807,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_152206,IEDB_153219,IEDB_244149,IEDB_885822,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_72
The structure is contained in the following publication(s):
- Article ID: 4585
Okan NA, Kasper DL "The atypical lipopolysaccharide of Francisella" -
Carbohydrate Research 378 (2013) 79-83
Bacterial lipopolysaccharides (LPSs) are ubiquitous molecules that are prominent components of the outer membranes of most gram-negative bacteria. Genetic and structural characterizations of Francisella LPS have revealed substantial differences when compared to more commonly studied LPSs of the Enterobacteriaceae. This review discusses both the general characteristics and the unusual features of Francisella LPS.
structure, O-antigen, lipid A, Francisella tularensis, Kdo hydrolase, lipopolysachharide
NCBI PubMed ID: 23916469Publication DOI: 10.1016/j.carres.2013.06.015Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: D.L. Kasper
Institutions: Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA, USA
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14. Compound ID: 11768
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b-D-Glcp-(1-2)-+
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a-D-Glcp-(1-3)-+ | Kdo-(2-?)-+
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a-D-GalpNAc-(1-2)-b-D-Manp-(1-4)-a-D-Manp-(1-5)-a-Kdop-(2--/lipid A/ |
Show graphically |
Structure type: oligomer
Aglycon: lipid A
Compound class: LOS
Contained glycoepitopes: IEDB_130648,IEDB_130650,IEDB_130658,IEDB_130659,IEDB_130701,IEDB_137473,IEDB_137485,IEDB_1391961,IEDB_141584,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_152206,IEDB_153219,IEDB_244149,IEDB_885822,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_72
The structure is contained in the following publication(s):
- Article ID: 4717
Rasmussen JA, Post DM, Gibson BW, Lindemann SR, Apicella MA, Meyerholz DK, Jones BD "Francisella tularensis Schu S4 LPS Core Sugar and O-antigen Mutants are Attenuated in a Mouse Model of Tularemia" -
Infection and Immunity 82(4) (2014) 1523-1539
The virulence factors mediating Francisella pathogenesis are being investigated with emphasis on understanding how the organism evades innate immunity mechanisms. F. tularensis produces a lipopolysaccharide (LPS) that is essentially inert and a polysaccharide capsule that helps the organism evade detection by components of innate immunity. We have identified, from a F. tularensis Schu S4 mutant library, strains that are disrupted for capsule and O-antigen production. These serum-sensitive strains lack both capsule production and O-antigen laddering. Analysis of the predicted protein sequences of the disrupted genes (FTT1236 and FTT1238c) revealed similarity to waa (rfa) biosynthetic genes in other bacteria. Mass spectrometry further revealed that these proteins are involved in LPS core sugar biosynthesis and the ligation of O-antigen to the LPS core sugars. The LD50 values of these strains are increased 100- to 1000-fold for mice. Histopathology revealed that the immune response to the F. tularensis mutant strains was significantly different than that observed with wild type infected mice. The lung tissue from mutant infected mice had widespread necrotic debris but the spleens lacked necrosis and displayed neutrophilia. In contrast, the lungs of wild type infected mice had nominal necrosis but the spleens had widespread necrosis. These data indicate that murine death caused by the wild type strains occurs by a mechanism different from that by which the mutant strains killed mice. Mice immunized with these mutant strains displayed greater than ten-fold protective effects against virulent type A F. tularensis challenge.
Lipopolysaccharide, O-antigen, Francisella tularensis, virulence factor
NCBI PubMed ID: 24452684Publication DOI: 10.1128/IAI.01640-13Journal NLM ID: 0246127Publisher: American Society for Microbiology
Correspondence: Bradley D. Jones
Institutions: Department of Microbiology, University of Iowa Carver College of Medicine, Iowa City, Iowa, USA, Sciences Division/Microbiology, Pacific Northwest National Laboratory, Richland, Washington, USAd, The Buck Institute for Age Research, Novato, California, USA, The Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, USA, Midwest Regional Center for Excellence in Biodefense and Emerging Infectious Disease Research, Washington University, St. Louis, Missouri, USA
Methods: GC-MS, de-O-acylation, ELISA, MALDI-MS, MS/MS, biological assays, composition analysis, genetic methods, immunoblotting, PAGE
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Total list of corresponding CSDB IDs (record IDs):
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