We conducted a structural analysis of the cell wall mannans isolated from two Candida tropicalis strains, IFO 0199 and IFO 1647, exhibiting strong agglutinabilities against anti-Candida factor sera 5 and 6. The products released from these mannans by acid treatment were identified as the oligosaccharides, from biose to pentaose, consisting solely of β-1,2-linked mannopyranose units corresponding to common epitopes of Candida albicans serotypes A and B (factor 5). Mild acetolysis of acid- and alkali-treated mannans produced large amounts of hexaose and heptaose, Man rho β-1-2Man rho β-1-2Man rho α 1-2Man rho α 1-2Man rho α 1-2Man and Man rho β-1-2Man rho β-1-2Man rho β-1-2Man rho α 1-2 Man rho α 1-2Man, corresponding to the C. albicans serotype A-specific epitopes (factor 6). However, the homologous pentaose, Man rho β-1-2Man rho α 1-2 Man, was not generated by this procedure. The oligosaccharides (biose to hexaose) obtained from the mannans by conventional acetolysis were composed exclusively of α-1,2-linked mannopyranose units. Therefore, the mannans of C. tropicalis IFO 0199 and IFO 1647 do not have the α-1,3-linked mannopyranose units previously observed in the mannans of C. albicans and Candida stellatoidea. The results of this study and previous findings indicate that the similarity of the antigenicities of three Candida species, C. albicans serotype A, C. stellatoidea type II, and C. tropicalis, reside in the β-1,2 and α-1,2 linkages containing oligomannosyl side chain (factor 6) in the cell wall mannan.
NCBI PubMed ID: 7507898Journal NLM ID: 0246127Publisher: American Society for Microbiology
Institutions: Second Department of Hygienic Chemistry, Tohoku College of Pharmacy, Miyagi, Japan
Methods: 1H NMR, acid hydrolysis, enzymatic digestion, acetolysis, slide agglutination reaction
Found