Show legend Show as text

Structure type: oligomer
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_114709,IEDB_115136,IEDB_130701,IEDB_135813,IEDB_137340,IEDB_140630,IEDB_141807,IEDB_144983,IEDB_151531,IEDB_152206,IEDB_423153,IEDB_983930,SB_44,SB_67,SB_72

• Article ID: 93
  Gamian A, Katzenellenbogen E, Romanowska E, Fernandez JMG, Pedersen C, Ulrich J, Defaye J "Structure of the Hafnia alvei strain PCM 1188 O-specific polysaccharide" - Carbohydrate Research 277 (1995) 245-255
  

  The lipopolysaccharide was extracted from cells of Hafnia alvei PCM 1188 strain and, after mild acid hydrolysis, the O-specific polysaccharide isolated and characterized. On the basis of sugar and methylation analysis, FAB mass spectrometry and NMR spectroscopy of the polysaccharide and oligosaccharides obtained after Smith degradation, or solvolysis with anhydrous hydrogen fluoride, the repeating unit of the O-specific polysaccharide was shown to be the pentasaccharide: [formula: see text]

  LPS, O-antigen, endotoxin, Hafnia alvei

  NCBI PubMed ID: 8556734
  Publication DOI: 10.1016/0008-6215(95)00200-D
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: gamian@immuno.iitd.pan.wroc.pl
  Institutions: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Wroclaw, Poland, Universidad de Sevillia, Departamento de Quimica Organica, Facultad de Quimica, Sevillie, Spain, The Technical University of Denmark, Institute of Organic Chemistry, Lyngby, Denmark, CNRS and CEA, Institut de Biologie Structurale, Grenoble, France, CNRS and CEA, Departament de Recherche Fondamentale sur la Matiere Condensee, Grenoble, France
  Methods: NMR-2D, methylation, FAB-MS, NMR, HF solvolysis, sugar analysis, Smith degradation
  
   
  
  Hafnia alvei PCM 1188
  CSDB ID 2202 (all data & tools)

Show legend Show as text

Structure type: oligomer
Contained glycoepitopes: IEDB_114709

• Article ID: 609
  Aspinall GO, Lynch CM, Pang H, Shaver RT, Moran AP "Chemical structures of the core region of Campylobacter jejuni O:3 lipopolysaccharide and an associated polysaccharide" - European Journal of Biochemistry 231 (1995) 570-578
  

  The complete structure for the core oligosaccharide region of the water-insoluble low-Mr, lipopolysaccharide of Campylobacter jejuni serotype 0:3 from phenol/water extraction of bacterial cells was assigned through studies on derivatives of the liberated oligosaccharide. Structure determinations were performed using 1H-NMR and 31P-NMR spectroscopies, methylation analysis supported by fast-atom-bombardment mass spectrometry, and Smith degradation experiments. It was concluded that the complete chains in the core oligosaccharide had the following structure in which a proportion of the terminal residues were phosphorylated: . From a similar series of experiments, it was concluded that an associated polysaccharide, which was isolated from the water phase of the phenoVwater extracts, had the following repeating unit in which a proportion of the previously unknown L-glycero-D-ido-heptose (L-a-D-ido-Hep) residues were present as 3-hydroxypropanoyl esters, and were not covalently linked to the lipopolysaccharide: -[→3)-L-a-D-Ido-Hep-(l→4)-a-D-Gal-(l-]n-.

  Lipopolysaccharide, LPS, structure, core, polysaccharide, O-antigen, Campylobacter, Campylobacter jejuni, core region, region

  NCBI PubMed ID: 7544281
  Journal NLM ID: 0107600
  Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
  Institutions: Department of Microbiology, University College, Galway, Ireland, Department of Chemistry, York University, Toronto, Ontario, Canada, Carbohydrate Research Centre, Department of Molecular and Medical Genetics, University of Toronto, Ontario, Canada
  Methods: deacetylation, NMR-2D, FAB-MS, GC-MS, dephosphorylation, GC, Smith degradation
  
   
  
  Campylobacter jejuni O3
  CSDB ID 118 (all data & tools)

Show legend Show as text

Structure type: oligomer
Contained glycoepitopes: IEDB_114709,IEDB_135813,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_141794,IEDB_141807,IEDB_151528,IEDB_151531,IEDB_190606,SB_173,SB_7

• Article ID: 606
  Arbatsky NP, Mamyan SS, Shashkov AS, Knirel YA, Kochetkov NK, Zych K, Sidorczyk Z "Structure of the O-specific polysaccharide of a serologically separate Proteus penneri strain 22" - Carbohydrate Research 310(1-2) (1998) 85-90
  

  The O-specific polysaccharide chain (O-antigen) of Proteus penneri strain 22 lipopolysaccharide was studied using chemical methods, including partial acid hydrolysis and Smith degradation, as well as one- and two-dimensional 1H and 13C NMR spectroscopy. The following structure of the pentasaccharide repeating unit was established: -4)bDGalp(1-3)[Ac(1-2)]bDGlcpN(1-3)[Ac(1-2)aDGalfN(1-4)bDGlcpA(1-4)]aDGalp(1-. The O-specific polysaccharide contains a GalNAc residue in the furanose form which has not been hitherto found in bacterial polysaccharides. The O-antigen studied is serologically and structurally unique among Proteus strains and, therefore, a new Proteus serogroup O63 is proposed for P. penneri strain 22.

  Lipopolysaccharide, O-antigen, Proteus penneri, O-Serogrouping, 2-Acetamido-2-deoxy-d-galactofuranose

  NCBI PubMed ID: 9794073
  Publication DOI: 10.1016/S0008-6215(98)00157-8
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: knirel@ioc.ac.ru
  Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Microbiology and Immunology, University of Lodz, 90-237 Lodz, Poland
  Methods: NMR-2D, acid hydrolysis, GLC, Smith degradation
  
   
  
  Proteus penneri 22
  CSDB ID 185 (all data & tools)

Show legend Show as text

Structure type: oligomer
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_114709,IEDB_142345

• Article ID: 675
  Hanniffy O, Shashkov AS, Senchenkova SN, Tomshich SV, Komandrova NA, Romanenko LA, Knirel YA, Savage AV "Structure of an acidic O-specific polysaccharide of Pseudoalteromonas haloplanktis type strain ATCC14393 containing 2-acetamido-2-deoxy-D- and -L-galacturonic acids and 3-(N-acetyl-D-alanyl)amino-3,6-dideoxy-D-glucose" - Carbohydrate Research 321(1-2) (1999) 132-138
  

  An acidic O-specific polysaccharide was obtained from the lipopolysaccharide of Pseudoalteromonas haloplanktis ATCC14393 and found to contain D-galactose, 3-(N-acetyl-D-alanyl)amino-3,6-dideoxy-D-glucose (DQui3NxDAlaAc), 2,4-diacetamido-2,4,6-trideoxy-D-glucose (D-QuiNAc4NAc), 2-acetamido-2-deoxy-D- and -L-galacturonic acids (D- and L-GalNAcA), and O-acetyl groups. On the basis of Smith degradation and 1H and 13C NMR spectroscopic studies, including 2D COSY, TOCSY, NOESY, 1H, 13C HMQC, and HMBC experiments, the following structure of the pentasaccharide repeating unit of the polysaccharide was established: -4)-a-L-GalpNAcA-(1-3)-b-D-QuipNAc4NAc-(1-2)-b-D-Quip3NxDAlaAc-(1-4)-a-D-GalpNAcA-(1-4)-a-D-Galp2,6Ac2-(1- where O-acetylation of the galactose residue at each position is partial (50-70%).

  NMR spectroscopy, O-Specific polysaccharide structure, 2, 4, Pseudoalteromonas haloplanktis, L-iduronic acid, 4-diamino-2, 6-trideoxy-D-glucose, (S)-3-hydroxybutyric acid

  Publication DOI: 10.1016/S0008-6215(97)10108-2
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: angela.savage@nuigalway.ie
  Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Department of Chemistry, National University of Ireland, Galway, Ireland, Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Vladivostok 690022, Russian Federation
  Methods: NMR-2D, NMR, Smith degradation
  
   
  
  Pseudoalteromonas haloplanktis ATCC 14393
  CSDB ID 576 (all data & tools)

Show legend Show as text

Structure type: oligomer
Contained glycoepitopes: IEDB_114709,IEDB_1394181,IEDB_145010

• Article ID: 763
  Cérantola S, Montrozier H "Structural elucidation of two polysaccharides present in the lipopolysaccharide of a clinical isolate of Burkholderia cepacia" - European Journal of Biochemistry 246(2) (1997) 360-366
  

  Based on the sugar composition, methylation analyses and Smith degradation, supported by NMR spectroscopic analyses and fast-atom-bombardment MS experiments, the lipopolysaccharide produced by a clinical isolate of Burkholderia cepacia was shown to contain two distinct polymers, both with linear trisaccharide repeating units; a major, containing D-rhamnose and D-galactose residues (2:1) with the structure →3)-α-D-Rhap(1→3)-α-D-Rhap(1→4)-α-D-Galp(1→ (major), and a minor repeating unit, constituted by D-rhamnosyl residues, with the structure →3)-α-D-Rhap(1→3)-α-D-Rhap(1→2)-α-D-Rhap(1→ (minor).

  Lipopolysaccharide, LPS, clinical, isolate, structural, polysaccharide, Burkholderia, Burkholderia cepacia, polysaccharides, elucidation, cystic fibrosis, D-rhamnose, fast-atom-bombardment MS

  NCBI PubMed ID: 9208925
  Journal NLM ID: 0107600
  Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
  Institutions: Institut de Pharmacologie et de Biologie Structurale du CNRS, Toulouse, France
  Methods: NMR-2D, methylation, NMR, sugar analysis, Smith degradation, partial depolymerization
  
   
  
  Burkholderia cepacia
  CSDB ID 1314 (all data & tools)

Show legend Show as text

Structure type: oligomer
Contained glycoepitopes: IEDB_114709,IEDB_135813,IEDB_136907,IEDB_137340,IEDB_141807,IEDB_151531

• Article ID: 940
  Linnerborg M, Wollin R, Widmalm G "Structural studies of the O-antigen polysaccharide from Escherichia coli O167" - European Journal of Biochemistry 246 (1997) 565-573
  

  The structure of the O-antigenic polysaccharide from Escherichia coli O167:H5 has been investigated. Sugar and methylation analyses, fast-atom-bombardment mass spectrometry and 1H- and 13C NMR spectroscopy were the main methods used. The structure of the repeating unit of the polysaccharide was found to be: [formula in text]. Oligosaccharide derivatives of the polysaccharide were obtained by HF solvolysis and by a Smith degradation. Furthermore, base treatment of the polysaccharide led to a degraded polymeric material. For the methylated polysaccharide the amide linkage between alanine and the galacturonic acid residue was reductively cleaved with LiBD4 in ethanol, to give, among other things, a 3-O-methyl galactose derivative.

  Lipopolysaccharide, NMR, LPS, structure, structural, polysaccharide, O-antigen, polysaccharides, O antigen, Escherichia, Escherichia coli, structural studies, galactofuranose, galacturonic acid, amide, L-alanine

  NCBI PubMed ID: 9208951
  Publication DOI: 10.1111/j.1432-1033.1997.00565.x
  Journal NLM ID: 0107600
  Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
  Institutions: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden, Swedish Insitute of Infectious Disease Control (SMI), Stockholm, Sweden
  Methods: NMR-2D, methylation, FAB-MS, partial acid hydrolysis, NMR, sugar analysis, Smith degradation
  
   
  
  Escherichia coli O167
  CSDB ID 3230 (all data & tools)

Show legend Show as text

Structure type: oligomer
Contained glycoepitopes: IEDB_114709,IEDB_130648,IEDB_137473,IEDB_1391961,IEDB_141584,IEDB_149136,IEDB_885822

• Article ID: 957
  MacLean LL, Perry MB "Structural characterization of the serotype O:5 O-polysaccharide antigen of the lipopolysaccharide of Escherichia coli O:5" - Biochemistry and Cell Biology 75(3) (1997) 199-205
  

  The structure of the antigenic O-polysaccharide component of the smooth lipopolysaccharide produced by Escherichia coli serotype O:5 was investigated by composition, methylation, and periodate oxidation methods, and by 1D and 2D nuclear magnetic resonance spectroscopy. The antigenic O-chain was determined to be a high molecular weight polysaccharide composed of repeating tetrasaccharide units containing 2-acetamido-2-deoxy-D-galactose, D-galactose, D-ribose, and 3-acetamido-3,6-dideoxy-D-glucose and having the structure -[-4)-β-D-Galp-(1-3)-α-D-GalpNAc-(1-4)-β-D-Quinp3NAc-(1- 3)-β-D-Ribf-(1-]-.

  Lipopolysaccharide, NMR, antigen, LPS, structural, characterization, polysaccharide, serotype, O antigen, Escherichia, Escherichia coli, O-polysaccharide, O polysaccharide

  NCBI PubMed ID: 9404639
  Journal NLM ID: 8606068
  Publisher: Ottawa: National Research Council of Canada
  Correspondence: malcolm.perry@nrc.ca
  Institutions: Institute for Biological Sciences, National Research Council, Ottawa, ON K1A OR6, Canada
  Methods: NMR-2D, methylation, NMR, composition analysis, periodate oxidation
  
   
  
  Escherichia coli O5
  CSDB ID 3232 (all data & tools)

Show legend Show as text

Structure type: monomer
Contained glycoepitopes: IEDB_114709,IEDB_130648,IEDB_137473

• Article ID: 1233
  Shin JEN, Ackloo S, Mainkar AS, Monteiro MA, Pang H, Penner JL, Aspinall GO "Lipo-oligosaccharides of Campylobacter jejuni serotype O:10. Structures of core oligosaccharide regions from a bacterial isolate from a patient with the Miller-Fisher syndrome and from the serotype reference strain" - Carbohydrate Research 305(2) (1998) 223-232
  

  Lipo-oligosaccharide (LOSa) was obtained by phenol-water extraction of bacterial cells of an isolate PG 836, identified as Campylobacter jejuni serotype O:10, from a patient who subsequently developed the Miller-Fisher syndrome (MFS). The product was separated into a water-insoluble gel of low Mr and a water-soluble component of high Mr. The structure of the core oligosaccharide region in LOSa is reported herein for comparison with LOSb from the C. jejuni O:10 reference strain, and is based on investigations carried out on: (1) O-deacylated LOSa; (2) the core oligosaccharide (OS 1a) liberated on acetic acid hydrolysis of the ketosidic linkages to lipid A, with accompanying loss of N-acetylneuraminic acid residues; (3) the product of the removal of phosphate residues from OS 1a to give OS 2a; and (4) the Smith degradation of OS 2a to yield a mixture of Os 3a and OS 4a. The results revealed that the core oligosaccharide region in LOSa from the MFS bacterial isolate had chains (1a), of which some were terminated by an N-acetylneuraminobiose [Neu5Ac(α 2-8)Neu5Ac] unit in a GD3 [Neu5Ac-Neu5Ac-Gal] epitope, and the inner regions of which were different from those of other C. jejuni serotypes. Similar experiments on LOSb from bacterial cells of the C. jejuni O:10 reference strain showed that the core oligosaccharide unit [1a, R = P (phosphoric monoester)] of LOSa from the MFS isolate was more uniformly complete than that of the O:10 reference strain [1b, R = AEP (2-aminoethylphosphate)] differing in the nature of the phosphate substituent at the inner heptose residue. The close structural relationship of LOSa from the MFS associated bacterium to LOSb from the O:10 reference strain runs parallel to that of the previously studied Guillain-Barré syndrome (GBS) associated bacterium typed as C. jejuni O:19 in comparison with the lipo-oligosaccharide from the reference strain. Preliminary studies on the high Mr components showed that those from the O:10 strains were indistinguishable from each other, but were structurally unrelated to those from the GBS associated C. jejuni serotype O:19 isolates and the O:19 reference strain [G.O. Aspinall, A.G. McDonald, and H. Pang, Biochemistry, 33 (1994) 250-255].

  Guillain-Barre syndrome, lipo-oligosaccharide, Miller-Fisher syndrome, Campylobacter jejuni serotype O:10, GD3 epitope, N-acetylneuraminobiose units

  NCBI PubMed ID: 9581276
  Publication DOI: 10.1016/s0008-6215(97)00259-0
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: aspinall@yorku.ca
  Institutions: Department of Chemistry, York University, North York, Toronto, Canada, Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario, Canada M5S 1A8, Department of Microbiology, University of Toronto, Toronto, Ontario, Canada M5S 1A8
  Methods: NMR
  
   
  
  Campylobacter jejuni O10 (PG836)
  CSDB ID 4763 (all data & tools)

Show legend Show as text

Structure type: oligomer
Contained glycoepitopes: IEDB_114709,IEDB_135813,IEDB_136095,IEDB_137340,IEDB_137472,IEDB_141807,IEDB_151531,IEDB_190606

• Article ID: 1243
  Sorum U, Robertsen B, Kenne L "Structural studies of the major polysaccharide in the cell wall of Renibacterium salmoninarum" - Carbohydrate Research 306(1-2) (1998) 305-314
  

  The galactose-rich polysaccharide (GPS) in the cell wall of the Gram-positive bacterium Renibacterium salmoninarum, the causative agent in of bacterial kidney disease (BKD) of salmonids, has been studied by sugar and methylation analysis, partial acid hydrolysis, Smith degradation, FABMS, and 1H and 13C NMR spectroscopy. The data show that the GPS has a heptasaccharide repeating unit with the following structure: α-D-Rhap-(1→3)-α-L-FucpNAc-(1→)-β-D-GlcpNAc 1 decreases 2 →3)-β-D-Galf-(1→6)-β-D-Galf-(1→3)-β-D-Galf-(1→6)-β-D-Galf-(1→.

  NMR, structure, cell wall polysaccharide, Renibacterium salmoninarum

  NCBI PubMed ID: 9691455
  Publication DOI: 10.1016/s0008-6215(97)10071-4
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Institutions: Department of Marine Biochemistry, The Norwegian College of Fishery Science, University of Tromsø, N-9037 Tromsø, Norway, Department of Chemistry, Swedish University of Agricultural Sciences, Box 7015, S-750 07 Uppsala, Sweden
  Methods: 13C NMR, 1H NMR, methylation, FAB-MS, partial acid hydrolysis, Smith degradation
  
   
  
  Renibacterium salmoninarum
  CSDB ID 5011 (all data & tools)

Show legend Show as text

Structure type: oligomer
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_114709,IEDB_130648,IEDB_136906,IEDB_137472,IEDB_137473,IEDB_141501,IEDB_141794,IEDB_151528,IEDB_190606,SB_21,SB_7

• Article ID: 1859
  Kocharova NA, Vinogradov EV, Knirel YA, Shashkov AS, Kochetkov NK, Stanislavsky ES, Kholodkova EV "The structure of the O-specific polysaccharide chains of the lipopolysaccharides of Citrobacter O32 and Salmonella arizonae O64 (Arizona 29)" - Bioorganicheskaya Khimia = Bioorganic Chemistry [Russian] 14(5) (1988) 697-700
  

  On the basis of acid hydrolysis, methylation, Smith degradation, selective cleavage with anhydrous hydrogen fluoride, and 13C NMR analysis, the repeating unit of the O-specific polysaccharide of Citrobacter O32 was concluded to have the following structure: (Formula: see text). The repeating unit of the Salmonella arizonae O64 O-specific polysaccharide has the same structure lacking the O-acetyl group.

  NCBI PubMed ID: 2458736
  Journal NLM ID: 7804941
  Publisher: Moskva: Nauka
  Institutions: N.D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the USSR, Moscow, Russia
  Methods: 13C NMR, HF solvolysis, Smith degradation, de-O-acetylation, NaBH4 reduction
  
   
  
  Citrobacter sp. O32, Salmonella arizonae O64
  CSDB ID 110645 (all data & tools)

Show legend Show as text

Structure type: oligomer
Compound class: CPS
Contained glycoepitopes: IEDB_114709,IEDB_136044,IEDB_137472,IEDB_141794,IEDB_141807,IEDB_142487,IEDB_142488,IEDB_146664,IEDB_151531,IEDB_190606,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_6,SB_7,SB_88

• Article ID: 1912
  Richards JC, Perry MB, Kniskern PJ "The structure of the specific capsular polysaccharide of Streptococcus pneumoniae type 11F (American type 11)" - Canadian Journal of Biochemistry and Cell Biology = Revue canadienne de biochimie et biologie cellulaire 63(12) (1985) 953-968
  

  The specific polysaccharide of Streptococcus pneumoniae type 11F (American type 11) is composed of 2-acetamido-2-deoxy-D-glucose (one part), D-glucose (one part), D-galactose (two parts), ribitol (one part), phosphate (one part), and O-acetyl (two parts). Hydrolysis, dephosphorylation, periodate oxidation, methylation, optical rotation, and 1H and 13C nuclear magnetic resonance studies showed that the polysaccharide is an unbranched linear polymer of a ribitol-phosphate substituted repeating tetrasaccharide unit having the structure: (Formula: see text). The specific capsular polysaccharides of S. pneumoniae type 11B and 11C (American types 76 and 53) were found to have the same tetrasaccharide repeating unit as the 11F polysaccharide, but differed from it in their mode of O-acetylation and the replacement of the ribitol phosphate by glycerol phosphate in the 11C specific polysaccharide.

  NCBI PubMed ID: 4075231
  Publication DOI: 10.1139/o85-118
  Journal NLM ID: 8302763
  Publisher: Ottawa: National Research Council Of Canada
  Institutions: Division of Biological Sciences, National Research Council of Canada, Ottawa, Ontario K1A OR6 Canada, Merck Sharp & Dohme Research Laboratories, West Point, PA, U.S.A. 19486
  Methods: gel filtration, 13C NMR, 1H NMR, methylation, GLC-MS, sugar analysis, dephosphorylation, TLC, GLC, paper chromatography, de-O-acetylation, ion-exchange chromatography, immunodiffusion assays, periodate oxidation, optical rotation measurement
  
   
  
  Streptococcus pneumoniae 11F
  CSDB ID 112148 (all data & tools)

Show legend Show as text

Structure type: oligomer
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_114709,IEDB_130648,IEDB_136906,IEDB_137472,IEDB_137473,IEDB_141501,IEDB_141794,IEDB_151528,IEDB_190606,SB_21,SB_7

• Article ID: 1859
  Kocharova NA, Vinogradov EV, Knirel YA, Shashkov AS, Kochetkov NK, Stanislavsky ES, Kholodkova EV "The structure of the O-specific polysaccharide chains of the lipopolysaccharides of Citrobacter O32 and Salmonella arizonae O64 (Arizona 29)" - Bioorganicheskaya Khimia = Bioorganic Chemistry [Russian] 14(5) (1988) 697-700
  

  On the basis of acid hydrolysis, methylation, Smith degradation, selective cleavage with anhydrous hydrogen fluoride, and 13C NMR analysis, the repeating unit of the O-specific polysaccharide of Citrobacter O32 was concluded to have the following structure: (Formula: see text). The repeating unit of the Salmonella arizonae O64 O-specific polysaccharide has the same structure lacking the O-acetyl group.

  NCBI PubMed ID: 2458736
  Journal NLM ID: 7804941
  Publisher: Moskva: Nauka
  Institutions: N.D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the USSR, Moscow, Russia
  Methods: 13C NMR, HF solvolysis, Smith degradation, de-O-acetylation, NaBH4 reduction
  
   
  
  Citrobacter sp. O32, Salmonella arizonae O64
  CSDB ID 110646 (all data & tools)

Show legend Show as text

Structure type: oligomer
Contained glycoepitopes: IEDB_114709,IEDB_130648,IEDB_136105,IEDB_137473,IEDB_1391961,IEDB_141584,IEDB_225177,IEDB_885822,IEDB_885823

• Article ID: 3499
  Kumirska J, Szafranek J, Czerwicka M, Golebiowski M, Paszkiewicz M, Dziadziuszko H, Kunikowska D, Stepnowski P "Smith degradation of the O-antigenic polysaccharide of Salmonella Dakar: structural studies of the products" - Carbohydrate Research 343(6) (2008) 1120-1125
  

  Two different oligosaccharides were obtained from the Smith degradation of the O-polysaccharide isolated from the lipopolysaccharide of Salmonella Dakar. The structures of these oligosaccharides were investigated by chemical analysis, NMR spectroscopy and MALDI-TOF mass spectrometry. The following structures of these products were determined: α-D-GalpNAc-(1→4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→2)-threitol and where Quip3NAc is 3-acetamido-3,6-dideoxyglucose. The reaction products confirmed the structure of the repeating unit of the Salmonella Dakar O-polysaccharide reported previously [Kumirska, J.; Szafranek, J.; Czerwicka, M.; Paszkiewicz, M.; Dziadziuszko, H.; Kunikowska, D.; Stepnowski, P. Carbohydr. Res. 2007,342, 2138-2143]

  NMR, structure, O-polysaccharide, mass spectrometry, Smith degradation, Salmonella Dakar

  NCBI PubMed ID: 18336805
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: kumirska@chem.univ.gda.pl
  Institutions: Faculty of Chemistry, University of Gdansk, Gdansk, Poland
  Methods: 13C NMR, 1H NMR, NMR-2D, GLC-MS, sugar analysis, acid hydrolysis, GLC, Smith degradation, MALDI-TOF MS, NMR-1D
  
   
  
  Salmonella sp. Dakar
  CSDB ID 22681 (all data & tools)

Show legend Show as text

Structure type: monomer
Contained glycoepitopes: IEDB_114709

• Article ID: 3690
  Ganguly J, Choudhury BP, Balakrish Nair G, Sen AK "Structural characterization of the O-antigenic polysaccharide from the lipopolysaccharide of Vibrio cholerae O37" - Indian Journal of Chemistry 48(5) (2009) 729-734
  

  The chemical structure of the O-antigenic polysaccharide isolated from the lipopolysaccharide of Vibrio cholerae O37 by mild acid hydrolysis was elucidated. The O-antigenic polysac­charide is found to consist of D-glucose, N-acetyl-D-Quinovos­amine and small amount of 4-O-methyl-N-acetyl-D-quinovas­amine. The structure of the O-antigen is established by using sugar and methylation analyses, Smith degradation studies and by using GLC, GC-MS, FAB-MS, one dimensional 1H and 13C NMR spectroscopy and two dimensional NMR spectroscopy including COSY, TOCSY, HSQC, experiments.

  Lipopolysaccharide, structural, characterization, polysaccharide, O-antigen, O-antigenic, O-antigenic polysaccharide, Vibrio, Vibrio cholerae, cholera, Vibrio cholerae O37

  Journal NLM ID: 7613423
  WWW link: http://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijcb/ijcb2k9/ijcb_may09.asp
  Correspondence: ganguly_jhuma@rediffmail.com; arsen@iicb.res.in
  Institutions: Department of Organic Chemistry (Carbohydrate), Indian Institute of Chemical Biology, 4, Raja S. C. Mullick Road, Jadavpur, Kolkata 700 032, India
  Methods: 13C NMR, 1H NMR, NMR-2D, methylation, FAB-MS, GC-MS, SDS-PAGE, sugar analysis, acid hydrolysis, GLC, Smith degradation
  
   
  
  Vibrio cholerae O37
  CSDB ID 23998 (all data & tools)

Show legend Show as text

Structure type: oligomer
Contained glycoepitopes: IEDB_114709

• Article ID: 3737
  MacLean LL, Perry MB, Chen W, Vinogradov E "The structure of the polysaccharide O-chain of the LPS from Acinetobacter baumannii strain ATCC 17961" - Carbohydrate Research 344(4) (2009) 474-478
  

  The gram-negative bacterium Acinetobacter baumannii strain ATCC17961 has been used by several laboratories in mouse models of respiratory A. baumannii infection, and a study of the role of its lipopolysaccharide in the pathogenicity is of interest. The structure of the O-deacylated polysaccharide O-chain component of its LPS has been determined by 2D NMR spectroscopy and mass spectrometry methods, and by the structural identification of oligosaccharides obtained by sequential application of the Smith degradation of the O-antigen. The O-chain was determined to be a polymer of a branched pentasaccharide repeating unit composed of 2,3-diacetamido-2,3-dideoxy-d-glucuronic acid, 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-d-galactose, d-glucose, and d-galactose, and has the following structure: (see text).

  Lipopolysaccharide, NMR, LPS, structure, polysaccharide, O-antigen, Acinetobacter, Baumannii

  NCBI PubMed ID: 19187931
  Publication DOI: 10.1016/j.carres.2008.12.026
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: E. Vinogradov
  Institutions: Institute for Biological Sciences, National Research Council Canada, 100 Sussex Dr., Ottawa, ON, Canada K1A 0R6
  Methods: 13C NMR, 1H NMR, NMR-2D, GLC-MS, HF solvolysis, SDS-PAGE, sugar analysis, ESI-MS, Smith degradation, NMR-1D, methanolysis, hydrazinolysis
  
   
  
  Acinetobacter baumannii ATCC 17961
  CSDB ID 24093 (all data & tools)