Found 3 structures.
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1. Compound ID: 5491
Kdop-(2-4)-+
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a-D-Galp-(1-4)-b-D-Galp-(1-4)-a-D-Glcp-(1-2)-b-D-Glcp-(1-6)-+ |
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a-D-Galp-(1-4)-b-D-Galp-(1-4)-a-D-GlcpNAc-(1-2)-b-D-Glcp-(1-4)-a-D-Glcp-(1-5)-a-Kdop
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b-D-Glcp-(1-3)-+ |
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Structure type: oligomer
Compound class: LOS
Contained glycoepitopes: IEDB_130650,IEDB_130651,IEDB_130659,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_140108,IEDB_141794,IEDB_141806,IEDB_141807,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_144991,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_152217,IEDB_158560,IEDB_190606,IEDB_742247,IEDB_983931,SB_165,SB_166,SB_177,SB_178,SB_187,SB_192,SB_195,SB_30,SB_31,SB_6,SB_62,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 50
Edebrink P, Jansson P, Jansson PE, Rahman MM, Widmalm G, Holme T, Rahman M "Structural studies of the O-antigen oligosaccharides from two strains of Moraxella catarrhalis serotype C" -
Carbohydrate Research 266 (1995) 237-261
The oligosaccharide parts from Moraxella (Branhamella) catarrhalis serotype C lipooligosaccharides were isolated by mild acid hydrolysis followed by gel permeation chromatography. Four different oligosaccharides could be identified from strain RS26 and two from strain RS10. The structures of the O-oligosaccharides were established by methylation analyses, mass spectrometry, and NMR spectroscopy. It is concluded that the oligosaccharide O-antigens from RS26 are a mixture of octa-, deca-, and undeca-saccharides, and most likely a heptasaccharide. Strain RS10 contains the deca- and the undeca-saccharide only. The structures for the oligosaccharides are shown below. [formula: see text] OS(7) [formula: see text] OS(8) [formula: see text] OS(10) [formula: see text] OS(11) Methylation analysis of the intact lipooligosaccharides showed that two Kdo residues were present, one terminal and one 4,5-substituted residue. It also showed that they consisted of a lipid A portion with 6-substituted glucosamine residues.
NMR, Lipooligosaccharide, Moraxella catarrhalis, Branhamella
NCBI PubMed ID: 7535189Publication DOI: 10.1016/0008-6215(94)00276-LJournal NLM ID: 0043535Publisher: Elsevier
Institutions: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden
Methods: NMR-2D, methylation, NMR, sugar analysis, MS
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2. Compound ID: 9407
a-Kdop-(2-4)-+
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a-D-Galp-(1-4)-b-D-Galp-(1-4)-a-D-Glcp-(1-2)-b-D-Glcp-(1-6)-+ |
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a-D-Galp-(1-4)-b-D-Galp-(1-4)-a-D-GlcpNAc-(1-2)-b-D-Glcp-(1-4)-a-D-Glcp-(1-5)-a-Kdop-(2--/lipid A/
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b-D-Glcp-(1-3)-+ |
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Structure type: oligomer
Aglycon: lipid A
Compound class: LPS
Contained glycoepitopes: IEDB_130650,IEDB_130651,IEDB_130659,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_140108,IEDB_141794,IEDB_141806,IEDB_141807,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_144991,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_152217,IEDB_158560,IEDB_190606,IEDB_742247,IEDB_983931,SB_165,SB_166,SB_177,SB_178,SB_187,SB_192,SB_195,SB_30,SB_31,SB_6,SB_62,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 4004
Cox AD, St Michael F, Cairns CM, Lacelle S, Filion AL, Neelamegan D, Wenzel CQ, Horan H, Richards JC "Investigating the potential of conserved inner core oligosaccharide regions of Moraxella catarrhalis lipopolysaccharide as vaccine antigens: accessibility and functional activity of monoclonal antibodies and glycoconjugate derived sera" -
Glycoconjugate Journal 28(3-4) (2011) 165-182
We investigated the conservation and antibody accessibility of inner core epitopes of Moraxella catarrhalis lipopolysaccharide (LPS) in order to assess their potential as vaccine candidates. Two LPS mutants, a single mutant designated lgt2 and a double mutant termed lgt2/lgt4, elaborating truncated inner core structures were generated in order to preclude expression of host-like outer core structures and to create an inner core structure that was shared by all three serotypes A, B and C of M. catarrhalis. Murine monoclonal antibodies (mAbs), designated MC2-1 and MC2-10 were obtained by immunising mice with the lgt2 mutant of M. catarrhalis serotype A strain. We showed that mAb MC2-1 can bind to the core LPS of wild-type (wt) serotype A, B and C organisms and concluded that mAb MC2-1 defines an immunogenic inner core epitope of M. catarrhalis LPS. We were unsuccessful in obtaining mAbs to the lgt2/lgt4 mutant. MAb MC2-10 only recognised the lgt2 mutant and the wt serotype A strain, and exhibited a strong requirement for the terminal N-acetyl-glucosamine residue of the lgt2 mutant core oligosaccharide, suggesting that this residue was immunodominant. Subsequently, we showed that both mAbs MC2-1 and MC2-10 could facilitate bactericidal killing of the lgt2 mutant, however neither mAb could facilitate bactericidal killing of the wt serotype A strain. We then confirmed and extended the candidacy of the inner core LPS by demonstrating that it is possible to elicit functional antibodies against M. catarrhalis wt strains following immunisation of rabbits with glycoconjugates elaborating the conserved inner core LPS antigen. The present study describes three conjugation strategies that either uses amidases produced by Dictyostelium discoideum, targeting the amino functionality created by the amidase activity as the attachment point on the LPS molecule, or a strong base treatment to remove all fatty acids from the LPS, thus creating amino functionalities in the lipid A region to conjugate via maleimide-thiol linker strategies targeting the carboxyl residues of the carrier protein and the free amino functionalities of the derived lipid A region of the carbohydrate resulted in a high loading of carbohydrates per carrier protein from these carbohydrate preparations. Immunisation derived antisera from rabbits recognised fully extended M. catarrhalis LPS and whole cells. Moreover, bactericidal activity was demonstrated to both the immunising carbohydrate antigen and importantly to wt cells, thus further supporting the consideration of inner core LPS as a potential vaccine antigen to combat disease caused by M. catarrhalis.
Lipopolysaccharide, epitope, lipid A, monoclonal antibodies, Moraxella catarrhalis, amidase
NCBI PubMed ID: 21590368Publication DOI: 10.1007/s10719-011-9332-7Journal NLM ID: 8603310Publisher: Kluwer Academic Publishers
Correspondence: Andrew.Cox@nrc-cnrc.gc.ca
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, ON, K1A 0R6, Canada.
Methods: 13C NMR, 1H NMR, NMR-2D, SDS-PAGE, DNA techniques, ELISA, MALDI-MS, Western blotting, de-O-acylation with hydrazine, NMR-1D, serological methods, de-N-O-acylation, alkaline hydrolysis, CE-ESI-MS, de-N-acylation
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3. Compound ID: 14535
a-Kdop-(2-4)-+
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a-D-Galp-(1-4)-b-D-Galp-(1-4)-a-D-Glcp-(1-2)-b-D-Glcp-(1-6)-+ |
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a-D-Galp-(1-4)-b-D-Galp-(1-4)-a-D-GlcpNAc-(1-2)-b-D-Glcp-(1-4)-a-D-Glcp-(1-5)-a-Kdop-(2--/(2->6)lipid A/
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b-D-Glcp-(1-3)-+ |
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Structure type: oligomer
Aglycon: (2->6)lipid A
Compound class: LOS
Contained glycoepitopes: IEDB_130650,IEDB_130651,IEDB_130659,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_140108,IEDB_141794,IEDB_141806,IEDB_141807,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_144991,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_152217,IEDB_158560,IEDB_190606,IEDB_742247,IEDB_983931,SB_165,SB_166,SB_177,SB_178,SB_187,SB_192,SB_195,SB_30,SB_31,SB_6,SB_62,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 5771
Gao Y, Lee J, Widmalm G, Im W "Preferred conformations of lipooligosaccharides and oligosaccharides of Moraxella catarrhalis" -
Glycobiology 30(2) (2020) 86-94
Moraxella catarrhalis (M. catarrhalis) is a pathogenic gram-negative bacterium that causes otitis media and sinusitis in children. Three major serotypes A, B and C are identified to account for approximately 95% of the clinical isolates. Understanding the conformational properties of different serotypes of M. catarrhalis provides insights into antigenic determinants. In this work, all-atom molecular dynamics simulations were conducted for M. catarrhalis lipooligosaccharide (LOS) bilayer systems and oligosaccharides (OS) in water solution to investigate the conformational similarities and differences of three serotypes. For up to 10 neutral monosaccharides in the core part, the conformational ensembles described by the pair-wise root mean square deviation distributions are similar among the three serotypes of either the LOS or OS. At the central β-(1→4)-linkage, anti-psi conformation in conjunction with the gauche-gauche (g-) conformation of the central trisubstituted glucosyl residue is observed as the dominant conformation to sustain the structural characteristics of M. catarrhalis three types, which is further supported by calculated transglycosidic 3{J}{C,H}({psiH}) of serotype A in comparison to experimental data. Interestingly, the conformational variability of three serotypes is more restricted for the OS in water solution than that in the LOS bilayer systems. The LOS-LOS interactions in the bilayer systems are responsible for the increased conformational diversity despite of tight packing. Solvent-accessible surface area analysis suggests that a trisaccharide attached to the β-(1→6)-linked sugar in all three serotypes of LOS could be the common epitope and have the possibility to interact with antibodies.
antigenic determinant, epitope, Gram-negative bacteria, engineering, molecular dynamics simulation, bacterial outer membrane
NCBI PubMed ID: 31616921Publication DOI: 10.1093/glycob/cwz086Journal NLM ID: 9104124Publisher: IRL Press at Oxford University Press
Correspondence: wonpil@lehigh.edu
Institutions: School of Mathematics, Physics and Statistics, Shanghai University of Engineering Science, 333 Longteng Road, Songjiang District, Shanghai 201620, China, Departments of Biological Sciences and Bioengineering, Lehigh University, 111 Research Drive, Bethlehem, PA 18015, USA, Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Svante Arrhenius vag 16C, SE-10691 Stockholm, Sweden, School of Computational Sciences, Korea Institute for Advanced Study, 85 Hoegiro, Dongdaemun-gu, Seoul 02455, Republic of Korea
Methods: NMR, conformation analysis, MD simulations, CHARMM-GUI Membrane Builder
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