Found 12 structures.
Displayed structures from 1 to 12
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1. Compound ID: 717
Structure type: polymer chemical repeating unit
; n=4
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_1394181,IEDB_141807,IEDB_145008,IEDB_151531
The structure is contained in the following publication(s):
- Article ID: 190
Vinogradov E, Petersen BO, Duus JO, Radziejewska-Lebrecht J "The structure of the polysaccharide part of the LPS from Serratia marcescens serotype O19, including linkage region to the core and the residue at the non-reducing end" -
Carbohydrate Research 338(23) (2003) 2757-2761
The structure of the LPS from Serratia marcescens serotype O19 was investigated. Deamination of the LPS released the O-chain polysaccharide together with a fragment of the core oligosaccharide. The following structure of the product was determined by NMR spectroscopy, mass spectrometry, and chemical methods: [carbohydrate structure: see text] The main polymer consists of a repeating disaccharide V-U and is present on average of 18 units per chain as estimated by integration of signals in the NMR spectra. The residue O corresponds to the primer, which initiates biosynthesis of the O-chain, and an oligomer of a disaccharide R-S is an insert between the primer and the main polymer. The polysaccharide has a β-Kdo residue at the non-reducing end, a feature similar to that observed previously in the LPS from Klebsiella O12.
LPS, structure, core, polysaccharide, serotype, linkage, Serratia marcescens, region, nonreducing, Serratia
NCBI PubMed ID: 14670734Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: evguenii.vinogradov@nrc-cnrc.gc.ca
Institutions: Carlsberg Laboratory, Department of Chemistry, Gamle Carlsberg Vej 10, DK-2500 Valby, Copenhagen, Denmark, Institute for Biological Sciences, National Research Council, 100 Sussex Dr., Ottawa, ON, Canada K1A 0R6, Uniwersytet Slaski, Katedra Mikrobiologii, ul. Jagiellonska 28, 40-032 Katowice, Poland
Methods: NMR-2D, NMR, chemical methods, MS
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2. Compound ID: 2908
|
b-D-GlcpNAc-(1-4)-+
|
-3)-a-D-Rhap-(1-3)-a-D-Rhap-(1-2)-a-D-Rhap-(1-2)-a-D-Rhap-(1- |
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Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_1394181,IEDB_141807,IEDB_144827,IEDB_145005,IEDB_145006,IEDB_145008,IEDB_145010,IEDB_151531
The structure is contained in the following publication(s):
- Article ID: 1038
Müller P, Zähringer U, Rudolph K "Induced resistance by bacterial lipopolysaccharides (LPS)" -
Proceedings of Plant Pathogenic Bacteria, International Conference (9th : 1996 : Madras, India) (1998) 569-575
Lipopolysaccharides (LPS) were extracted from the Pseudomonas syringae pathovars: tomato race 1 (GSPB Nr. 1778), tomato race 0 (GSPB Nr. 1776), and glycinea race 9 (GSPB Nr. 1986) by the phenol/chloroform/petroleumbenzene-method (Galanos -et al 1979). The chemical analysis revealed 10% contamination by levan, a polyfructose, but also high amounts of KDO, PO4 (3-), GlcNAc and rhamnose. Chemical structure-analysis identified the O-specific chain, the-core region and lipid A. Lipid A was composed of the typical fatty acids of Pseudomonas such as 3-OH-C 10:0, C12:0, 3-OH C 12:0 and 2-OH C12:0. The structure of the O-chain corresponded to the one reported by Zdovorvenko et al. 1992, typical for Pseudomonas syringae pv. glycinea. Determination of the core-region has not been completely finished, but seems to show strong similarities to Pseudomonas aeruginosa and P. fluorescens. Induced resistance was studied in tomato and tobacco leaves. We investigated the role of whole LPS as well as its subunits O-chain and core region on induced resistance in compatible and incompatible systems. Resistance was induced by 50 jig LPS/ml in the incompatible system (LPS of Pseudomonas syringae pv. glycinea race 9 against P. s. pv. tomato race 1 in tomato cv. "Lyconorma"). Neither the O-specific chain nor the core region alone induced resistance. When similar experiments were performed by pretreatment with P.s. pv. tomato LPS followed by inoculation with P. s. pv. tomato (compatible system) resistance was not induced. Tobacco leaves were pretreated with 50-1000 uxj LPS/ml of P. s. pv. glycinea race 9 followed by inoculation with 108cfu/ml P. s. pv. glycinea race 9 48 h later. The pretreatment delayed appearance of the HR from 20 to 60 hrs after bacterial inoculation. The O-chain or the coreregion did not cause this effect. Further experiments have to show why the plants can differentiate between compatible and incompatible LPS, and whether the complete LPS-molecule is necessary for resistance induction or whether lipid A alone can cause this effect.
Lipopolysaccharide, lipopolysaccharides, LPS, structure, core, Bacterial, lipid A, induced, O polysaccharide, bacteria, Pseudomonas syringae, composition, resistance, plant, pathogenic bacteria, pathogenic, HR
Correspondence: PMUELLE@GWDG.DE
Editors: Mahadevan A
Institutions: Institut fur Phytopathologie and Pflanzenschutz der Universitat Gottingen Grisebachstr. 6, D-37077 Gottingen, Germany, Zentrum fur Medizin and Biowissenschaften, Borstel, Germany
Methods: sugar analysis
- Article ID: 1777
Knirel YA, Kochetkov NK "The structure of lipopolysaccharides of gram-negative bacteria. III. The structure of O-antigens: A review" -
Biochemistry (Moscow) 59(12) (1994) 1325-1383
This review summarizes data on the composition and structure of the O-antigens, the polysaccharide chains of the outer-membrane lipopolysaccharides (LPS) of Gram-negative bacteria defining the immunospecificity of these microbial cells. Special reference is given to some structural features of the O-antigens, such as the presence of unique monosaccharides and noncarbohydrate components, masked regularity, and the occurrence in one microorganism of LPS with structurally different polysaccharide chains. Antigenic relationships between microorganisms belonging to different taxonomic groups are discussed.
structure, O-antigen, chemical composition, bacterial lipopolysaccharides, Salmonella livingstone C1
NCBI PubMed ID: 7533007Journal NLM ID: 0376536Publisher: Nauka/Interperiodica
Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
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3. Compound ID: 2979
|
b-D-GlcpNAc-(1-4)-+
|
-3)-a-D-Rhap-(1-3)-a-D-Rhap-(1-2)-a-D-Rhap-(1-2)-a-D-Rhap-(1- |
Show graphically |
Structure type: polymer chemical repeating unit
Trivial name: D-rhamnan
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_1394181,IEDB_141807,IEDB_144827,IEDB_144829,IEDB_145005,IEDB_145006,IEDB_145008,IEDB_145010,IEDB_151531
The structure is contained in the following publication(s):
- Article ID: 1071
Ovod V, Rudolph K, Knirel YA, Krohn K "Immunochemical characterization of O polysaccharides composing the a-D-rhamnose backbone of lipopolysaccharide of Pseudomonas syringae and classification of bacteria into serogroups O1 and O2 with monoclonal antibodies" -
Journal of Bacteriology 178 (1996) 6459-6465
Murine monoclonal antibodies (MAbs) reacting with Pseudomonas syringae lipopolysaccharide (LPS) O polysaccharides (OPS) composed of tetra- and tri-α-D-rhamnose repeats in the backbone [3)D-Rha(α1-3)D-Rha(α1-2)D-Rha(α1-2)D-Rha(α1] and [3)D-Rha(α1-3)D-Rha(α1-2)D-Rha(α1] were generated and used for immunochemical analysis and for serological classification of the bacteria. A total of 195 of 358 P. syringae strains tested representing 21 pathovars were shown to share a common epitope, 1a, and were classified into serogroup O1. All strains with pathovars aptata, glycinea, japonica, phaseolicola, and pisi, most of the strains with pathovars atrofaciens and striafaciens, and half of the strains with pathovar syringae were classified into serotypes O1a', O1b, O1c, and O1d within serogroup O1. Serogroup-specific epitope 1a was inferred to be related to the (α1-2)D-Rha(α1-3) site of the OPS backbone. The serotype-specific epitopes 1b, 1c, 1d, and 1a' were inferred as relating to the immunodominant lateral (α1-3)D-Rha, (β1-4)D-GlcNAc, and (α1-4)D-Fuc substituents and backbone-located site (α1-3)D-Rha(α1-2), respectively, of OPSs that share the common tetra-D-rhamnose repeats in the backbone. A total of 7.3% of the strains studied, all with pathovars morsprunorum and lapsa, were classified as serotypes O2a and O2d within serogroup 02. Serotype-specific epitope 2a was inferred as being related to the backbone-located site D-Rha(α1-3)D-Rha and epitope 2d to the immunodominant lateral (α1-4)D-Fuc residue of OPS consisting of tri-D-rhamnose repeats in the backbone. Epitope 2d alternated with 2a within the same LPS molecule and did not cross-react with epitope 1d. Serotypes O2a and O2d were observed in some strains correlating with the coexpression of the two chemotypes of OPS by the same strain. The serogroup O1-specific MAb Ps1a reacted weakly but definitely with all strains from serogroup 02. We propose serological formulas for serogroups O1 and 02 as well as for individual strains within these serogroups.
Lipopolysaccharide, LPS, characterization, polysaccharide, polysaccharides, Pseudomonas, antibodies, antibody, epitope, monoclonal, monoclonal antibodies, monoclonal antibody, O-polysaccharide, O polysaccharide, bacteria, serogroup, backbone, immunochemical, Pseudomonas syringae, classification, D-rhamnose
NCBI PubMed ID: 8932301Journal NLM ID: 2985120RPublisher: American Society for Microbiology
Correspondence: Ltvlov@uta.fi
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Medical Technology, University of Tampere, Tampere, Finland, Department of Microbiology and Immunology, University of Kiev, Kiev, Ukraine, Institut fur Pflanzenpathologie und Pflanzenschutz der Georg-August-Universitat, Gottingen, Germany
Methods: serological methods
- Article ID: 1073
Ovod V, Knirel Y, Krohn K "Demonstration of the immunochemical diversity of O-chains of lipopolysaccharide of Pseudomonas syringae and inferring of the serogroup- and serotype-specific epitopes with monoclonal antibodies" -
Proceedings of International Conference on Pseudomonas syringae Pathovars and Related Pathogens (5th : 1995 : Berlin, Germany) (1997) Vol. 9, 532-537
Using serogroup- and serotyppe-specific murine monoclonal antibodies (MAbs) to Pseudomonas syringae lipopolysacharide (LPS) O-polysaccharides (OPS) (=O chains) with elucidated primary chemical structure of the O-repeating units, a rather high diversity of the OPS-related epitopes was demonstrated and most of them were inferred. The immunogenic properties of the O-serogroup- and O-serotype-specific epitopes were shown to depend on the nature and the number of sugar residues in the O-repeat as well as on the arrangement of the monosaccharides and the mode of linkages between them.
Lipopolysaccharide, LPS, structure, strain, Pseudomonas, chain, group, antibodies, antibody, epitope, monoclonal, monoclonal antibodies, monoclonal antibody, epitopes, O-polysaccharide, serogroup, immunochemical, O-chain, pathogen, pathogens, pathovar, Pseudomonas syringae, classification, diversity, serotype-specific
Publisher: Kluwer Academic Publishers, The Netherlands
Correspondence: knirel@ioc.ac.ru
Editors: Rudolph K, Burr TJ, Mansfield JW, Stead DE, Vivian A, von Kietzell J
Institutions: Institute of Medical Technology, University of Tampere, Tampere, Finland, N.D. Zelinsy Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
- Article ID: 1320
Zdorovenko GM, Shashkov AS, Zdorovenko EL, Kocharova NA, Yakovleva LM, Knirel YA, Rudolph K "Characterization of the lipopolysaccharide and structure of the O-specific polysaccharide of the bacterium Pseudomonas syringae pv. atrofaciens IMV 948" -
Biochemistry (Moscow) 66(4) (2001) 369-377
Lipopolysaccharide (LPS) was isolated from the phytopathogenic bacterium Pseudomonas syringae pv. atrofaciens IMV 948 by mild extraction of the microbial cells with saline, and the properties, composition, and structure of the LPS were studied. The LPS showed low toxicity in D- galactosamine-sensitized mice and low biological activity in plants. Structural components of LPS--lipid A, core oligosaccharide, and O-specific polysaccharide (OPS)--were obtained by mild acid degradation and characterized. The lipid A contained fatty acids 3-HO-C10:0, C12:0, 2-HO-C12:0, 3-HO-C12:0, C16:0, C16:1, C18:0, and C18:1, as well as components of the hydrophilic moiety: GlcN, ethanolamine, phosphate, and phosphoethanolamine. The LPS core contained components typical of pseudomonads: glucose, rhamnose (Rha), L-glycero-D-manno-heptose, GlcN, GalN, 2-keto-3-deoxy-D-manno-octonic acid, alanine, and phosphate. The OPS consisted of L-Rha and D-GlcNAc in the ratio 4 : 1 and was structurally heterogeneous. The main pentasaccharide repeating unit of the OPS has the following structure: [structure see text]. Immunochemical studies showed that P. syringae pv. atrofaciens IMV 948 is serologically separate from other P. syringae strains, including those that have structurally similar OPS
Lipopolysaccharide, structure, Pseudomonas syringae, composition, immunochemistry, omposition
NCBI PubMed ID: 11403642Journal NLM ID: 0376536Publisher: Nauka/Interperiodica
Correspondence: zdorov@i.kiev.ua
Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kiev, 252143, Ukraine, Institut fur Pflazenpathologie und Pfalnzenschutz, Georg-August-Universitat, Gottingen, 37077, Germany
Methods: NMR-2D, methylation, NMR
- Article ID: 1452
Corsaro MM, De Castro C, Molinaro A, Parrilli M "Structure of lipopolysaccharides from phytopathogenic Gram-negative bacteria" -
Book: Recent Research Developments in Phytochemistry (2001) Vol. 5, 119-138
This review collects the structural data of lipopolysaccharide components arising from all phytopathogenic bacteria so far investigated. The structural approaches and the main biological role of these macromolecules are also reported.
Lipopolysaccharide, lipopolysaccharides, structure, core, lipid A, O-polysaccharide, gram negative bacteria
WWW link: https://books.google.ru/books/about/Recent_Research_Developments_in_Phytoche.html?id=5CJacgAACAAJ&redir_esc=yPublisher: Research Signpost, Trivandrum, India
Editors: Pandalai SG
Institutions: Dipartimento di Chimica Organica e Biochimica, Complesso Universitario Monte S.Angelo Via Cintia, 4, 80126 Napoli, Italy
- Article ID: 1465
Knirel YA, Zdorovenko GM "Structures of O-polysaccharide chains of lipopolysaccharides as the basis for classification of Pseudomonas syringae and related strains" -
Book: Pseudomonas Syringae Pathovars and Related Pathogens (series: Developments in Plant Pathology) (1997) 475-480
The O-polysaccharides of various serogroups of P. syringae were found to have similar structures with the main chain of a rhamnan which may carry a monosaccharide side chain of D-rhamnose, D-fucose, 2-acetamido-2-deoxy-D-glucose or 3-acetamido-3,6-dideoxy-D-galactose. The relationship between the serological specificity and the host-plant specificity of P. syringae and the structures of the O-polysaccharides is discussed.
Lipopolysaccharide, structure, O-antigen, O-polysaccharide, serological specificity, Pseudomonas syringae, Serogrouping, Host-plant specificity
Publication DOI: 10.1007/978-94-011-5472-7_85Publisher: Springer Netherlands
Editors: Rudolph K, Burr TJ, Mansfield JW, Stead D, Vivian A, von Kietzell J
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Leninsky Pr. 47, Moscow B-334, Russia, D.K. Zabolotny Institute of Microbiology and Virology, Zabolotnogo 154, Kiev-143, Ukraine
- Article ID: 1834
Knirel YA, Zdorovenko GM, Dashunin VM, Yakovleva LM, Shashkov AS, Zakharova IY, Gvozdyak RI, Kochetkov NK "Antigenic polysaccharides of bacteria. 15. Structure of the repeating unit of O-specific polysaccharide chain of Pseudomonas wieringae lipopolysaccharide" -
Bioorganicheskaya Khimia = Bioorganic Chemistry [Russian] 12(9) (1986) 1253-1262
No abstract available
Journal NLM ID: 7804941WWW link: http://www.rjbc.ru/arc/12/9/1253-1262.pdfPublisher: Moskva: Nauka
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the USSR, Moscow, Russia
Methods: 13C NMR, 1H NMR
- Article ID: 2789
Ovod V, Ashorn P, Yakovleva L, Krohn K "Classification of Pseudomonas syringae with monoclonal antibodies against the core and O-side chains of the lipopolysaccharide" -
Phytopathology 85 (1995) 226-232
Journal NLM ID: 9427222Publisher: American Phytopathological Society
- Article ID: 5242
Zdorovenko EL, Besarab NV, Shashkov AS, Novik GI, Shirokov AA, Burov AM, Knirel YA "Investigation of O-polysaccharides from bacterial strains of Pseudomonas genus as potential receptors of bacteriophage BIM BV-45." -
International Journal of Biological Macromolecules 118 (2018) 1065-1072
The structure of potential bacteriophage receptors located on cell walls of Gram-negative bacteria deposited at Belarusian collection of microorganisms was investigated. Studies by 1D and 2D 1H and 13C NMR spectroscopy enabled to elucidate the structure of the O-specific polysaccharides (OPS) constituting lipopolysaccharide (LPS) of some Pseudomonas species. The capacity of bacteriophage to adsorb to LPS molecules was tested.
Pseudomonas, NMR spectroscopy, Bacteriophages, electron microscopy, Adsorption, O-polysaccharides (OPS)
Publication DOI: 10.1016/j.ijbiomac.2018.06.165Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: zdorovenkoe@mail.ru
Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Microbiology, National Academy of Sciences of Belarus, 220141 Minsk, Belarus, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 410049, Saratov, Russian Federation
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, acid hydrolysis, mild acid hydrolysis, GC, GPC, analysis of bacteriophage morphology, analysis of the bacteriophages adsorption
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4. Compound ID: 3001
|
b-D-GlcpNAc-(1-4)-+
|
-3)-b-L-Rhap-(1-4)-a-L-Rhap-(1-3)-a-D-Rhap-(1- |
Show graphically |
Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_135813,IEDB_136105,IEDB_137340,IEDB_1394181,IEDB_141807,IEDB_145008,IEDB_151531,IEDB_225177,IEDB_885823
The structure is contained in the following publication(s):
- Article ID: 1073
Ovod V, Knirel Y, Krohn K "Demonstration of the immunochemical diversity of O-chains of lipopolysaccharide of Pseudomonas syringae and inferring of the serogroup- and serotype-specific epitopes with monoclonal antibodies" -
Proceedings of International Conference on Pseudomonas syringae Pathovars and Related Pathogens (5th : 1995 : Berlin, Germany) (1997) Vol. 9, 532-537
Using serogroup- and serotyppe-specific murine monoclonal antibodies (MAbs) to Pseudomonas syringae lipopolysacharide (LPS) O-polysaccharides (OPS) (=O chains) with elucidated primary chemical structure of the O-repeating units, a rather high diversity of the OPS-related epitopes was demonstrated and most of them were inferred. The immunogenic properties of the O-serogroup- and O-serotype-specific epitopes were shown to depend on the nature and the number of sugar residues in the O-repeat as well as on the arrangement of the monosaccharides and the mode of linkages between them.
Lipopolysaccharide, LPS, structure, strain, Pseudomonas, chain, group, antibodies, antibody, epitope, monoclonal, monoclonal antibodies, monoclonal antibody, epitopes, O-polysaccharide, serogroup, immunochemical, O-chain, pathogen, pathogens, pathovar, Pseudomonas syringae, classification, diversity, serotype-specific
Publisher: Kluwer Academic Publishers, The Netherlands
Correspondence: knirel@ioc.ac.ru
Editors: Rudolph K, Burr TJ, Mansfield JW, Stead DE, Vivian A, von Kietzell J
Institutions: Institute of Medical Technology, University of Tampere, Tampere, Finland, N.D. Zelinsy Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
- Article ID: 1452
Corsaro MM, De Castro C, Molinaro A, Parrilli M "Structure of lipopolysaccharides from phytopathogenic Gram-negative bacteria" -
Book: Recent Research Developments in Phytochemistry (2001) Vol. 5, 119-138
This review collects the structural data of lipopolysaccharide components arising from all phytopathogenic bacteria so far investigated. The structural approaches and the main biological role of these macromolecules are also reported.
Lipopolysaccharide, lipopolysaccharides, structure, core, lipid A, O-polysaccharide, gram negative bacteria
WWW link: https://books.google.ru/books/about/Recent_Research_Developments_in_Phytoche.html?id=5CJacgAACAAJ&redir_esc=yPublisher: Research Signpost, Trivandrum, India
Editors: Pandalai SG
Institutions: Dipartimento di Chimica Organica e Biochimica, Complesso Universitario Monte S.Angelo Via Cintia, 4, 80126 Napoli, Italy
- Article ID: 1777
Knirel YA, Kochetkov NK "The structure of lipopolysaccharides of gram-negative bacteria. III. The structure of O-antigens: A review" -
Biochemistry (Moscow) 59(12) (1994) 1325-1383
This review summarizes data on the composition and structure of the O-antigens, the polysaccharide chains of the outer-membrane lipopolysaccharides (LPS) of Gram-negative bacteria defining the immunospecificity of these microbial cells. Special reference is given to some structural features of the O-antigens, such as the presence of unique monosaccharides and noncarbohydrate components, masked regularity, and the occurrence in one microorganism of LPS with structurally different polysaccharide chains. Antigenic relationships between microorganisms belonging to different taxonomic groups are discussed.
structure, O-antigen, chemical composition, bacterial lipopolysaccharides, Salmonella livingstone C1
NCBI PubMed ID: 7533007Journal NLM ID: 0376536Publisher: Nauka/Interperiodica
Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
- Article ID: 2190
Vinogradov EV, Shashkov AS, Knirel YA, Zdorovenko GM, Solyanik LP, Gubanova NY, Yakovleva LM "Somatic antigens of pseudomonads: structure of the O-specific polysaccharide chain of Pseudomonas syringae pv. tabaci 225 (serogroup VIII) lipopolysaccharide" -
Carbohydrate Research 212 (1991) 307-311
No abstract available
NCBI PubMed ID: 1720348Publication DOI: 10.1016/0008-6215(91)84071-lJournal NLM ID: 0043535Publisher: Elsevier
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the U.S.S.R., Moscow
Methods: 13C NMR, methylation, GLC-MS, GLC, de-N-acetylation/deamination
- Article ID: 3338
Spitali M, Smith ARW "Structure of the Lipopolysaccharide Side Chain of Pseudomonas syringae pv. tabaci Strain NCPPB 79 (?CFBP 1615), in Relation to O-serogroup" -
Phytopathologische Zeitschrift = Journal of Phytopathology 155(1) (2007) 1-7
The lipopolysaccharide (LPS) side chain from Pseudomonas syringae pv. tabaci strain NCPPB 79 (=CFBP 1615) contained l- and d-rhamnose, and GlcNAc. Using methylation analysis, periodate oxidation, Smith degradation and 1H- and 13C-nuclear magnetic resonance spectroscopy, the repeat unit was found to have the structure: 3)[bGlcNac(1-4)]-a-D-Rhap(1-3)-b-L-Rhap(1-4)-a-L-Rhap(1- . This structure is correlated with a previously proposed serogrouping system. The involvement of LPS generally in plant disease is briefly discussed.
Lipopolysaccharide, structure, strain, side chain, Pseudomonas syringae, Serogrouping, Pseudomonas syringae pv. tabaci
Publication DOI: 10.1111/j.1439-0434.2006.01159.xJournal NLM ID: 9875585Publisher: Berlin: Parey
Correspondence: SmaA672@aol.com
Institutions: Department of Life Sciences, University of Greenwich at Medway, Central Avenue, Chatham Maritime, Kent ME4 4TB, UK
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, acid hydrolysis, GC, Smith degradation, NMR-1D, serological methods, periodate oxidation
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5. Compound ID: 3372
|
b-D-GlcNAc-(1-4)-+
|
-3)-a-D-Rhap-(1-3)-a-D-Rhap-(1-2)-a-D-Rhap-(1-2)-a-D-Rhap-(1- |
Show graphically |
Structure type: polymer chemical repeating unit
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_1394181,IEDB_141807,IEDB_144827,IEDB_144829,IEDB_145005,IEDB_145006,IEDB_145008,IEDB_145010,IEDB_151531
The structure is contained in the following publication(s):
- Article ID: 1247
Spitali M, Rees N, Wait R, Smith ARW "Structures of lipopolysaccharide side-chains of NCPPB 871, the neopathotype strain of Pseudomonas syringae pv aptata, strain 299A of pv pisi and NCPPB 52, the neopathotype strain of pv phaseolicola, in relation to O-serogroup" -
Phytopathologische Zeitschrift = Journal of Phytopathology 143(11-12) (1995) 663-669
The side-chains of lipopolysaccharides from Pseudomonas syringae pvs. aptata, pisi and phaseolicola contain D-rhamnose. Using 'H- and I3C-nuclear magnetic resonance spectroscopy and methylation analysis, the side-chains of NCPPB 871, the neopathotype strain of pv. aptata, and strain 299 A of pv. pisi race 1 were found to be of identical structure: [formula: see text]. Side-chains from NCPPB 52, the neopathotype strain of pv. phaseolicola, were based on the same backbone structure, but were substituted to the extent of approximately 67+ACU- with branch D-fucose residues: [formula: see text]. Side-chain structures are correlated with a previously proposed ser-ogrouping system.
Lipopolysaccharide, structure, strain, Pseudomonas, side chain, Pseudomonas syringae, O-serotype
Journal NLM ID: 9875585Publisher: Berlin: Parey
Institutions: School of Biological and Chemical Sciences, University of Greenwich, London SE18 6PF, UK, Public Health Laboratory Service, Centre for Applied Microbiology Research, Porton Down, Salisbury SP4 OJG, UK, School of Biological and Chemical Sciences, University of Greenwich, London SE18 6PF, UK, Public Health Laboratory Service, Centre for Applied Microbiology Research, Porton Down, Salisbury SP4 OJG, UK
Methods: 13C NMR, 1H NMR, methylation, serological methods
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6. Compound ID: 3460
|
b-D-GlcpNAc-(1-4)-+
|
-3)-a-L-Rhap-(1-4)-b-L-Rhap-(1-3)-a-D-Rhap-(1- |
Show graphically |
Structure type: polymer chemical repeating unit
Trivial name: rhamnan
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_135813,IEDB_136105,IEDB_137340,IEDB_1394181,IEDB_141807,IEDB_145008,IEDB_151531,IEDB_225177,IEDB_885823
The structure is contained in the following publication(s):
- Article ID: 1318
Zdorovenko GM, Solyanic LP, Yakovleva LM, Paramonov NA "Characterization of O-antigens from different strains of Pseudomonas syringae pv. tabaci" -
Biochemistry (Moscow) 62(1) (1997) 28-37
O-Antigens (lipopolysaccharides, LPS) were isolated by NaCl extraction from microbial biomass of Pseudomonas syringae pv. tabaci and purified by ultracentrifugation. Individual structural components of the LPS macromolecule (O-specific polysaccharide (O-PS), core oligosaccharide, and lipid A) were obtained and characterized. Fatty acids 3-OH-C10:0, C12:0, 2-OH-C12:0, 3-OH-C12:0, C16:1, C16:0, C18:1, and C18:0 were identified in the lipid A composition. Glucosamine, ethanolamine, and phosphoethanolamine were found in the hydrophilic part of the lipid A macromolecule in all strains tested. Lipid A preparations contained phosphorus and amino acids. Rhamnose, glucose, glucosamine, 2-keto-3-deoxyoctulosonic acid, heptose, alanine, and phosphorus were identified as the main core components. The strains differed in O-PS structure. We describe the O-chain of LPS in strain P-28. It contains repeating units of the following structure: [formula: see text] The O-PS structures of LPS from strains P-28 and 225 are identical, however, they differ substantially from that of strain 223. Both structures from strains 223 and 225 were reported previously. Antibodies to antigenic epitopes of O-PS, core, and lipid A were revealed in O-serum against the whole bacterial cells. Correlation of O-PS structure with the serological grouping of strains was observed
Lipopolysaccharide, core, O-antigen, lipid A, Pseudomonas syringae, O-antigenlipopolysaccharide, O-specific polyaccharide, composition and structure of lipoplysaccharide
NCBI PubMed ID: 9113726Journal NLM ID: 0376536Publisher: Nauka/Interperiodica
Correspondence: zdorov@i.kiev.ua
Institutions: Zabolotnyi Institute of Microbiology and Virology, National Academy of Sciences of Ukraine
Methods: NMR-2D, methylation, NMR
- Article ID: 1465
Knirel YA, Zdorovenko GM "Structures of O-polysaccharide chains of lipopolysaccharides as the basis for classification of Pseudomonas syringae and related strains" -
Book: Pseudomonas Syringae Pathovars and Related Pathogens (series: Developments in Plant Pathology) (1997) 475-480
The O-polysaccharides of various serogroups of P. syringae were found to have similar structures with the main chain of a rhamnan which may carry a monosaccharide side chain of D-rhamnose, D-fucose, 2-acetamido-2-deoxy-D-glucose or 3-acetamido-3,6-dideoxy-D-galactose. The relationship between the serological specificity and the host-plant specificity of P. syringae and the structures of the O-polysaccharides is discussed.
Lipopolysaccharide, structure, O-antigen, O-polysaccharide, serological specificity, Pseudomonas syringae, Serogrouping, Host-plant specificity
Publication DOI: 10.1007/978-94-011-5472-7_85Publisher: Springer Netherlands
Editors: Rudolph K, Burr TJ, Mansfield JW, Stead D, Vivian A, von Kietzell J
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Leninsky Pr. 47, Moscow B-334, Russia, D.K. Zabolotny Institute of Microbiology and Virology, Zabolotnogo 154, Kiev-143, Ukraine
- Article ID: 3313
Zdorovenko GM, Zdorovenko EL, Varbanets LD "Composition, structure, and biological properties of lipopolysaccharides from different strains of Pseudomonas syringae pv. atrofaciens" -
Mikrobiologiia = Microbiology [Russian] 76(6) (2007) 683-697
The composition, structure, and certain biological properties of lipopolysaccharides (LPS) isolated from six strains of bacteria Pseudomonas syringae pv.atrofaciens pathogenic for grain-crops (wheat, rye) are presented. The LPS-protein complexes were isolated by a sparing procedure (extraction from microbial cells with a weak salt solution). They reacted with the homologous O sera and contained one to three antigenic determinants. Against the cells of warm-blooded animals (mice, humans) they exhibited the biological activity typical of endotoxins (stimulation of cytokine production, mitogenetic activity, etc.). The LCD of the biovar type strain was highly toxic to mice sensitized with D-galactosamine. The structural components of LPS macromolecules obtained by mild acidic degradation were characterized: lipid A, core oligosaccharide, and O-specific polysaccharide (OPS). Fatty acids 3-HO-C10:0, C12:0, 2-HO-C12:0, 3-HO-C12:0, C16:0, C16:1, C18:0, and C18:1 were identified in lipid A of all the strains, as well as the components of the hydrophilic part: glucosamine (GlcN), ethanolamine (EtN), phosphate, and phosphoethanolamine (EtN-P). In the core LPS, glucose (Glc), rhamnose(Rha), L-glycero-D-manno-heptose (Hep), GlcN, galactosamine (GalN), 2-keto-3-deoxy-D-mannooctonoi acid (KDO), alanine (Ala), and phosphate were present. The O chain of all the strains consisted of repeated elements containing a linear chain of three to four L- (two strains) or D-Rha (four strains) residues supplemented with a single residue of 3-acetamido-3,6-dideoxy-D-galactose (D-Fucp3Nac), N-acetyl-D-glucosamine(D-GlcpNAc), D-fucose (D-Fucf), or D-Rhap (strain-dependent) as a side substituent. In different strains the substitution position for Rha residues in the repeated components of the major rhamnan chain was also different.One strain exhibited a unique type of O-chain heterogeneity. Immunochemical investigation of the LPS antigenic properties revealed the absence of close serological relations between the strains of one pathovar; this finding correlates with the differences in their OPS structure. Resemblance between the investigated strains and other P.syringae strains with similar LPS structures was revealed. The results of LPS analysis indicate the absence of correlation between the OPS structure and the pathovar affiliation of the strains.
Lipopolysaccharide, structure, lipid A, core oligosaccharide, O-specific polysaccharide, biological activity, immunochemistry, Pseudomonas syringae pv.atrofaciens
NCBI PubMed ID: 18297868Journal NLM ID: 0376652Publisher: Moskva: Izdatelstvo Nauka
Correspondence: alz@i.com.ua; evelina@ioc.ac.ru
Institutions: Zabolotnyi Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kiev, Zelinskii Institute of Organic Chemistry, Russian Academy of Sciences, Moscow
Methods: 13C NMR, 1H NMR, GLC-MS, sugar analysis, serological methods
- Article ID: 3968
Zdorovenko GM, Zdorovenko EL "Pseudomonas syringae lipopolysaccharides: Immunochemical characteristics and structure as a basis for strain classification" -
Mikrobiologiia = Microbiology [Russian] 79(1) (2010) 47-57
Lipopolysaccharide (LPS) preparations of 34 Pseudomonas syringae strains of 19 pathovars were prepared by saline extraction from wet cells and purified by repeated ultracentrifugation. The preparations reacted with homologous O-antisera, obtained by rabbit immunization with heat-killed bacterial cells. Through inhibition of homologous reactions between LPS preparations of heterologous strains (enzyme immunoassay, EIA), it was established for the first time that high serological affinity between strains is observed only if their LPS contains O-specific polysaccharide chains (OPS) comprised of completely identical rather than partially similar units. The central linear part of the OPS was found to be serologically inert when shielded with side groups. Data on immunochemical characteristics of the LPS and OPS structure are analyzed in relation to the design of P. syringae classification scheme.
Lipopolysaccharide, structure, O-specific polysaccharide, Pseudomonas syringae, classification, immunochemistry
NCBI PubMed ID: 20411661Publication DOI: 10.1134/S0026261710010078Journal NLM ID: 0376652Publisher: Moskva: Izdatelstvo Nauka
Correspondence: evelina@ioc.ac.ru
Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Zabolotnyi Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, ul. Zabolotnogo 154, Kyiv, 03143 Ukraine
Methods: partial acid hydrolysis, EIA, serological methods, de-N-acetylation/deamination
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7. Compound ID: 4836
Structure type: oligomer
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_1394181,IEDB_141807,IEDB_145008,IEDB_145010,IEDB_151531
The structure is contained in the following publication(s):
- Article ID: 1834
Knirel YA, Zdorovenko GM, Dashunin VM, Yakovleva LM, Shashkov AS, Zakharova IY, Gvozdyak RI, Kochetkov NK "Antigenic polysaccharides of bacteria. 15. Structure of the repeating unit of O-specific polysaccharide chain of Pseudomonas wieringae lipopolysaccharide" -
Bioorganicheskaya Khimia = Bioorganic Chemistry [Russian] 12(9) (1986) 1253-1262
No abstract available
Journal NLM ID: 7804941WWW link: http://www.rjbc.ru/arc/12/9/1253-1262.pdfPublisher: Moskva: Nauka
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the USSR, Moscow, Russia
Methods: 13C NMR, 1H NMR
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8. Compound ID: 5441
Structure type: fragment of a bigger structure
Contained glycoepitopes: IEDB_135813,IEDB_136105,IEDB_137340,IEDB_1394181,IEDB_141807,IEDB_145008,IEDB_151531,IEDB_225177,IEDB_885823
The structure is contained in the following publication(s):
- Article ID: 2276
Daffé M, McNeil M, Brennan PJ "Major structural features of the cell wall arabinogalactans of Mycobacterium, Rhodococcus, and Nocardia spp." -
Carbohydrate Research 249 (1993) 383-398
The cell wall arabinogalactans of strains of Mycobacterium, Rhodococcus, and Nocardia were per-O-methylated, partially hydrolyzed with acid, and the resulting oligosaccharides were reduced and per-O-ethylated to yield per-O-alkylated oligoglycosyl alditol fragments. Analyses of these fragments by gas chromatography-mass spectrometry and of the intact solubilized polysaccharides by 1H and 13C NMR revealed the major structural features of the different arabinogalactans from representatives of the different genera. All of the mycobacterial products contained a homogalactan segment of alternating 5-linked α-Galactofuranosyl (Galf) and 6-linked β-Galf residues. The arabinan segment consisted of three major domains, linear 5-linked α-arabinofuranosyl (Araf) residues and branched (3→5)-linked Araf units substituted with either 5-linked Araf or the disaccharide β-Araf-(1→2)-α-Araf at both branched positions. The recognition of these features in in vivo grown Mycobacterium leprae is an important development. The arabinan from strains of Nocardia contains a nonreducing-end motif composed of the linear trisaccharide, β-Araf-(1→2)-α-Araf-(1→5)-Araf, attached to linear 5-linked α-Araf units. The galactan segment of the arabinogalactan of Nocardia sp. is composed of linear 5-linked β-Galf units substituted in part at O-6 with terminal β-glucosyl units. The two representative strains of Rhodococcus also differed in the composition of the galactan moiety; in addition to the 5-linked Galf, 2- and 3-linked β-Galf units are present. The reducing end of the galactans, and therefore, apparently, of the entire arabinogalactans from all species from all genera, are apparently composed of the unit, rhamnosyl-(1→3)-N-acetyl-glucosamine, which, in turn, is apparently attached to peptidoglycan via phosphodiester linkage.
NCBI PubMed ID: 8275507Publication DOI: 10.1016/0008-6215(93)84102-CJournal NLM ID: 0043535Publisher: Elsevier
Institutions: Department of Microbiology, Colorado State University, Fort Collins 80523
Methods: 13C NMR, 1H NMR, GLC-MS
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9. Compound ID: 7305
|
?%b-D-GlcpNAc-(1-4)-+
|
-2)-a-D-Rhap-(1-3)-a-D-Rhap-(1-3)-a-D-Rhap-(1-2)-a-D-Rhap-(1- |
Show graphically |
Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_1394181,IEDB_141807,IEDB_144827,IEDB_144829,IEDB_145005,IEDB_145006,IEDB_145008,IEDB_145010,IEDB_151531
The structure is contained in the following publication(s):
- Article ID: 3313
Zdorovenko GM, Zdorovenko EL, Varbanets LD "Composition, structure, and biological properties of lipopolysaccharides from different strains of Pseudomonas syringae pv. atrofaciens" -
Mikrobiologiia = Microbiology [Russian] 76(6) (2007) 683-697
The composition, structure, and certain biological properties of lipopolysaccharides (LPS) isolated from six strains of bacteria Pseudomonas syringae pv.atrofaciens pathogenic for grain-crops (wheat, rye) are presented. The LPS-protein complexes were isolated by a sparing procedure (extraction from microbial cells with a weak salt solution). They reacted with the homologous O sera and contained one to three antigenic determinants. Against the cells of warm-blooded animals (mice, humans) they exhibited the biological activity typical of endotoxins (stimulation of cytokine production, mitogenetic activity, etc.). The LCD of the biovar type strain was highly toxic to mice sensitized with D-galactosamine. The structural components of LPS macromolecules obtained by mild acidic degradation were characterized: lipid A, core oligosaccharide, and O-specific polysaccharide (OPS). Fatty acids 3-HO-C10:0, C12:0, 2-HO-C12:0, 3-HO-C12:0, C16:0, C16:1, C18:0, and C18:1 were identified in lipid A of all the strains, as well as the components of the hydrophilic part: glucosamine (GlcN), ethanolamine (EtN), phosphate, and phosphoethanolamine (EtN-P). In the core LPS, glucose (Glc), rhamnose(Rha), L-glycero-D-manno-heptose (Hep), GlcN, galactosamine (GalN), 2-keto-3-deoxy-D-mannooctonoi acid (KDO), alanine (Ala), and phosphate were present. The O chain of all the strains consisted of repeated elements containing a linear chain of three to four L- (two strains) or D-Rha (four strains) residues supplemented with a single residue of 3-acetamido-3,6-dideoxy-D-galactose (D-Fucp3Nac), N-acetyl-D-glucosamine(D-GlcpNAc), D-fucose (D-Fucf), or D-Rhap (strain-dependent) as a side substituent. In different strains the substitution position for Rha residues in the repeated components of the major rhamnan chain was also different.One strain exhibited a unique type of O-chain heterogeneity. Immunochemical investigation of the LPS antigenic properties revealed the absence of close serological relations between the strains of one pathovar; this finding correlates with the differences in their OPS structure. Resemblance between the investigated strains and other P.syringae strains with similar LPS structures was revealed. The results of LPS analysis indicate the absence of correlation between the OPS structure and the pathovar affiliation of the strains.
Lipopolysaccharide, structure, lipid A, core oligosaccharide, O-specific polysaccharide, biological activity, immunochemistry, Pseudomonas syringae pv.atrofaciens
NCBI PubMed ID: 18297868Journal NLM ID: 0376652Publisher: Moskva: Izdatelstvo Nauka
Correspondence: alz@i.com.ua; evelina@ioc.ac.ru
Institutions: Zabolotnyi Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kiev, Zelinskii Institute of Organic Chemistry, Russian Academy of Sciences, Moscow
Methods: 13C NMR, 1H NMR, GLC-MS, sugar analysis, serological methods
- Article ID: 3968
Zdorovenko GM, Zdorovenko EL "Pseudomonas syringae lipopolysaccharides: Immunochemical characteristics and structure as a basis for strain classification" -
Mikrobiologiia = Microbiology [Russian] 79(1) (2010) 47-57
Lipopolysaccharide (LPS) preparations of 34 Pseudomonas syringae strains of 19 pathovars were prepared by saline extraction from wet cells and purified by repeated ultracentrifugation. The preparations reacted with homologous O-antisera, obtained by rabbit immunization with heat-killed bacterial cells. Through inhibition of homologous reactions between LPS preparations of heterologous strains (enzyme immunoassay, EIA), it was established for the first time that high serological affinity between strains is observed only if their LPS contains O-specific polysaccharide chains (OPS) comprised of completely identical rather than partially similar units. The central linear part of the OPS was found to be serologically inert when shielded with side groups. Data on immunochemical characteristics of the LPS and OPS structure are analyzed in relation to the design of P. syringae classification scheme.
Lipopolysaccharide, structure, O-specific polysaccharide, Pseudomonas syringae, classification, immunochemistry
NCBI PubMed ID: 20411661Publication DOI: 10.1134/S0026261710010078Journal NLM ID: 0376652Publisher: Moskva: Izdatelstvo Nauka
Correspondence: evelina@ioc.ac.ru
Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Zabolotnyi Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, ul. Zabolotnogo 154, Kyiv, 03143 Ukraine
Methods: partial acid hydrolysis, EIA, serological methods, de-N-acetylation/deamination
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10. Compound ID: 7366
Structure type: oligomer
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_1394181,IEDB_141807,IEDB_145008,IEDB_151531,IEDB_225177,IEDB_885823
The structure is contained in the following publication(s):
- Article ID: 3338
Spitali M, Smith ARW "Structure of the Lipopolysaccharide Side Chain of Pseudomonas syringae pv. tabaci Strain NCPPB 79 (?CFBP 1615), in Relation to O-serogroup" -
Phytopathologische Zeitschrift = Journal of Phytopathology 155(1) (2007) 1-7
The lipopolysaccharide (LPS) side chain from Pseudomonas syringae pv. tabaci strain NCPPB 79 (=CFBP 1615) contained l- and d-rhamnose, and GlcNAc. Using methylation analysis, periodate oxidation, Smith degradation and 1H- and 13C-nuclear magnetic resonance spectroscopy, the repeat unit was found to have the structure: 3)[bGlcNac(1-4)]-a-D-Rhap(1-3)-b-L-Rhap(1-4)-a-L-Rhap(1- . This structure is correlated with a previously proposed serogrouping system. The involvement of LPS generally in plant disease is briefly discussed.
Lipopolysaccharide, structure, strain, side chain, Pseudomonas syringae, Serogrouping, Pseudomonas syringae pv. tabaci
Publication DOI: 10.1111/j.1439-0434.2006.01159.xJournal NLM ID: 9875585Publisher: Berlin: Parey
Correspondence: SmaA672@aol.com
Institutions: Department of Life Sciences, University of Greenwich at Medway, Central Avenue, Chatham Maritime, Kent ME4 4TB, UK
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, acid hydrolysis, GC, Smith degradation, NMR-1D, serological methods, periodate oxidation
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11. Compound ID: 10092
|
Galp-(1-3)-Rhap-(1-3)-GlcpNAc-(1-4)-+
|
-2)-Rhap-(1-2)-Rhap-(1-?)-D-Glc-ol-(1-P- |
Show graphically |
Structure type: polymer chemical repeating unit
Compound class: CPS
Contained glycoepitopes: IEDB_114708,IEDB_133754,IEDB_135813,IEDB_136044,IEDB_136105,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_1394181,IEDB_141794,IEDB_141807,IEDB_145008,IEDB_151528,IEDB_151531,IEDB_190606,IEDB_225177,IEDB_885823,SB_165,SB_166,SB_187,SB_195,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 4194
Pritchard DG, Gray BM, Dillon HC "Characterization of the group-specific polysaccharide of group B Streptococcus" -
Archives of Biochemistry and Biophysics 235 (1984) 385-392
The group-specific polysaccharide of the group B Streptococcus was isolated by nitrous acid extraction followed by gel filtration on Sepharose 6B and chromatography on DEAE-Bio-Gel A. It was composed of rhamnose, galactose, N-acetylglucosamine, and glucitol phosphate. Mild periodate oxidation of the polysaccharide resulted in a rapid reduction in molecular weight, indicating that the glucitol was located in the backbone of the polymer. High-resolution 31P NMR showed the presence of a single type of phosphodiester bond in the molecule. Methylation analysis and several specific chemical degradations were done to determine sugar linkages. The basic structure of the group B polysaccharide consists of a backbone of 2-linked rhamnose, 2,4-linked rhamnose, and glucitol phosphate, and side chains of rhamnose(1→3)galactose(1→3)N-acetylglucosamine linked to the 4-position of a rhamnose in the backbone.
NCBI PubMed ID: 6097185Publication DOI: 10.1016/0003-9861(84)90211-XJournal NLM ID: 0372430Institutions: Departments of Microbiology and Pediatrics, University of Alabama in Birmingham, Birmingham, Alabama, U.S.A.
Methods: gel filtration, methylation, partial acid hydrolysis, GC-MS, TLC, 31P NMR, GC, radiolabeling, periodate oxidation
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12. Compound ID: 10364
Structure type: polymer chemical repeating unit
Contained glycoepitopes: IEDB_135813,IEDB_136105,IEDB_137340,IEDB_1394181,IEDB_141807,IEDB_145008,IEDB_151531,IEDB_225177,IEDB_885823
The structure is contained in the following publication(s):
- Article ID: 4306
Greenfield LK, Whitfield C "Synthesis of lipopolysaccharide O-antigens by ABC transporter-dependent pathways" -
Carbohydrate Research 356 (2012) 12-24
The O-polysaccharide (O-PS; O-antigen) of bacterial lipopolysaccharides is made up of repeating units of one or more sugar residues and displays remarkable structural diversity. Despite the structural variations, there are only three strategies for O-PS assembly. The ATP-binding cassette (ABC)-transporter-dependent mechanism of O-PS biosynthesis is widespread. The Escherichia coli O9a and Klebsiella pneumoniae O2a antigens provide prototypes, which are distinguished by the fine details that link glycan polymerization and chain termination at the cytoplasmic face of the inner membrane to its export via the ABC transporter. Here, we describe the current understanding of these processes. Since glycoconjugate assembly complexes that utilize an ABC transporter-dependent pathway are widespread among the bacterial kingdom, the models described here are expected to extend beyond O-PS biosynthesis systems
Lipopolysaccharide, O-polysaccharide, ATP-binding cassette transporter, Escherichia coli O9a, Klebsiella pneumoniae O2a
NCBI PubMed ID: 22475157Publication DOI: 10.1016/j.carres.2012.02.027Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: C. Whitfield
Institutions: Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1
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Total list of corresponding CSDB IDs (record IDs):
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