Found 12 structures.
Displayed structures from 1 to 12
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1. Compound ID: 86
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Subst-(1-5)-+
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a-Kdop-(2-4)-a-Ko-(2--/lipid A/
Subst = core oligosaccharide;
Ko = D-glycero-a-D-talo-oct-2-ulosonic acid |
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Structure type: oligomer
Aglycon: lipid A
Contained glycoepitopes: IEDB_130650,IEDB_135608,IEDB_135609
The structure is contained in the following publication(s):
- Article ID: 17
Bode CE, Brabetz W, Brade H "Cloning and characterization of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) transferase genes (kdtA) from Acinetobacter baumannii and Acinetobacter haemolyticus" -
European Journal of Biochemistry 254(2) (1998) 404-412
3-Deoxy-D-manno-oct-2-ulosonic acid (Kdo) transferases (KdtA) are multifunctional glycosyltransferases with primary structures of low similarity. Totally degenerated primers were deduced from two stretches of identical amino acids between known KdtA sequences and used to amplify by PCR a kdtA-specific fragment from Acinetobacter baumannii ATCC 15308 DNA which was then applied as a probe for the cloning and sequencing of the complete Kdo transferase gene. With conserved PCR primers for this structural gene from A. baumannii ATCC 15308, also kdtA genes of A. baumannii ATCC 19606 and A. haemolyticus ATCC 17906 were obtained, cloned from the chromosome and sequenced. The genes coded for proteins with similarities to known Kdo transferases. Within the genus Acinetobacter, the identity and similarity of the deduced amino acid sequences were 71% and 84.5%, respectively. The kdtA sequences of both A. baumannii strains were identical and possessed a TTG start codon, whereas ATG was found in the case of A. haemolyticus. The genes from Acinetobacter and kdtA from Escherichia coli K-12 were expressed in the Gram-positive bacterium Corynebacterium glutamicum. In vitro tests confirmed the function of the gene products as Kdo transferases, which transferred mainly two Kdo residues to a synthetic lipid A precursor of E. coli. Also, no differences between the cloned kdtA genes from A. baumanniii, A. haemnolyticus and E. coli were observed when tetraacyl or hexaacyl lipid A were tested, since all transferases acted more efficiently on the former. With limiting amounts of acceptor, all Kdo transferases were able to transfer a third Kdo residue with varying efficiency
gene, characterization, 3-deoxy-D-manno-oct-2-ulosonic acid, acid, Acinetobacter, Acinetobacter baumannii, Acinetobacter haemolyticus, cloning, Kdo, lipopolysaccharide biosynthesis, shuttle plasmid, transferase
NCBI PubMed ID: 9660198Publication DOI: 10.1046/j.1432-1327.1998.2540404.xJournal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Correspondence: hbrade@fz-borstel.de
Institutions: Division of Medical and Biochemical Microbiology, Research Center Borstel, Center for Medicine and Biosciences, Borstel, Germany
Methods: PCR, DNA sequencing, DNA cloning
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2. Compound ID: 735
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a-Kdop-(2-4)-+
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P-6)-+ | P-4)-+
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a-D-3dlyxHepp-ulosaric-(2-6)-b-D-Glcp-(1-4)-a-D-3dlyxHepp-ulosaric-(2-6)-a-D-Glcp-(1-6)-a-D-Glcp-(1-6)-b-D-Glcp-(1-4)-a-D-Glcp-(1-5)-a-Kop-(2-6)-b-D-GlcpN-(1-6)-a-D-GlcpN-(1-P |
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Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130650,IEDB_135394,IEDB_135608,IEDB_135609,IEDB_137340,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_144999,IEDB_146664,IEDB_151531,IEDB_241118,IEDB_983931,SB_192
The structure is contained in the following publication(s):
- Article ID: 195
Vinogradov EV, Müller-Loennies S, Petersen BO, Meshkov S, Thomas-Oates JE, Holst O, Brade H "Structural investigation of the lipopolysaccharide from Acinetobacter haemolyticus strain NCTC 10305 (ATCC 17906, DNA group 4)" -
European Journal of Biochemistry 247(1) (1997) 82-90
The structure of the lipopolysaccharide (LPS) from Acinetobacer haemolyticus strain NCTC 10305 (DNA group 4) was elucidated by means of analytical chemistry, NMR spectroscopy and fast-atom-bombardment mass spectrometry. Several oligosaccharides were obtained after deacylation or successive de-O-acylation, dephosphorylation, reduction, and de-N-acylation of LPS. In the major fraction of the LPS, the core is attached to the lipid A through D-glycero-D-talo-2-octulopyranosonic acid (Ko), whereas in a minor fraction (<20%) Ko is replaced by 3-deoxy-D-manno-octulopyranosonic acid (Kdo). The structures of the phosphorylated carbohydrate backbones of these LPS fractions are [see formula in text] with Dha = 3-deoxy-D-lyxo-2-heptulosaric acid, Sug = sugar and is Ko in a major fraction and Kdo in a minor fraction. All sugar residues have the D-configuration and are present in the pyranose form. Mass spectrometry of de-O-acylated LPS revealed the presence of and additional hexose residue in minor amounts, the position and nature of which could not be identified.
Lipopolysaccharide, NMR, structure, Acinetobacter, core-lipid A region
NCBI PubMed ID: 9249012Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Institutions: Department of Chemistry, Carlsberg Laboratory, Copenhagen, Denmark, Division of Medical and Microbiology, Center for Medicine and Biosciences, Research Center Borstel, Germany, Insitute of Chemical Physics, Russian Academy of Sciences, Moscow, Russia, Department of Mass Spectrometry, Bijvoet Center for Biomolecular Research, Universiteit Utrecht, The Netherlands
Methods: NMR-2D, FAB-MS, NMR, analytical methods
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3. Compound ID: 739
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a-Kdop-(2-4)-+ P-4)-+
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a-D-3dlyxHepp-ulosaric-(2-6)-b-D-Glcp-(1-4)-a-D-3dlyxHepp-ulosaric-(2-6)-a-D-Glcp-(1-6)-a-D-Glcp-(1-6)-b-D-Glcp-(1-4)-a-D-Glcp-(1-5)-a-Kop-(2-6)-b-D-GlcpN-(1-6)-a-D-GlcpN-(1-P |
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Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130650,IEDB_135394,IEDB_135608,IEDB_135609,IEDB_137340,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_983931,SB_192
The structure is contained in the following publication(s):
- Article ID: 195
Vinogradov EV, Müller-Loennies S, Petersen BO, Meshkov S, Thomas-Oates JE, Holst O, Brade H "Structural investigation of the lipopolysaccharide from Acinetobacter haemolyticus strain NCTC 10305 (ATCC 17906, DNA group 4)" -
European Journal of Biochemistry 247(1) (1997) 82-90
The structure of the lipopolysaccharide (LPS) from Acinetobacer haemolyticus strain NCTC 10305 (DNA group 4) was elucidated by means of analytical chemistry, NMR spectroscopy and fast-atom-bombardment mass spectrometry. Several oligosaccharides were obtained after deacylation or successive de-O-acylation, dephosphorylation, reduction, and de-N-acylation of LPS. In the major fraction of the LPS, the core is attached to the lipid A through D-glycero-D-talo-2-octulopyranosonic acid (Ko), whereas in a minor fraction (<20%) Ko is replaced by 3-deoxy-D-manno-octulopyranosonic acid (Kdo). The structures of the phosphorylated carbohydrate backbones of these LPS fractions are [see formula in text] with Dha = 3-deoxy-D-lyxo-2-heptulosaric acid, Sug = sugar and is Ko in a major fraction and Kdo in a minor fraction. All sugar residues have the D-configuration and are present in the pyranose form. Mass spectrometry of de-O-acylated LPS revealed the presence of and additional hexose residue in minor amounts, the position and nature of which could not be identified.
Lipopolysaccharide, NMR, structure, Acinetobacter, core-lipid A region
NCBI PubMed ID: 9249012Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Institutions: Department of Chemistry, Carlsberg Laboratory, Copenhagen, Denmark, Division of Medical and Microbiology, Center for Medicine and Biosciences, Research Center Borstel, Germany, Insitute of Chemical Physics, Russian Academy of Sciences, Moscow, Russia, Department of Mass Spectrometry, Bijvoet Center for Biomolecular Research, Universiteit Utrecht, The Netherlands
Methods: NMR-2D, FAB-MS, NMR, analytical methods
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4. Compound ID: 741
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a-Kdop-(2-4)-+
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P-6)-+ | P-4)-+
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a-D-Glcp-(1-6)-a-D-Glcp-(1-6)-b-D-Glcp-(1-4)-a-D-Glcp-(1-5)-a-Kop-(2-6)-b-D-GlcpN-(1-6)-a-D-GlcpN-(1-P |
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Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130650,IEDB_135394,IEDB_135608,IEDB_135609,IEDB_137340,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_144999,IEDB_146664,IEDB_151531,IEDB_241118,IEDB_983931,SB_192
The structure is contained in the following publication(s):
- Article ID: 195
Vinogradov EV, Müller-Loennies S, Petersen BO, Meshkov S, Thomas-Oates JE, Holst O, Brade H "Structural investigation of the lipopolysaccharide from Acinetobacter haemolyticus strain NCTC 10305 (ATCC 17906, DNA group 4)" -
European Journal of Biochemistry 247(1) (1997) 82-90
The structure of the lipopolysaccharide (LPS) from Acinetobacer haemolyticus strain NCTC 10305 (DNA group 4) was elucidated by means of analytical chemistry, NMR spectroscopy and fast-atom-bombardment mass spectrometry. Several oligosaccharides were obtained after deacylation or successive de-O-acylation, dephosphorylation, reduction, and de-N-acylation of LPS. In the major fraction of the LPS, the core is attached to the lipid A through D-glycero-D-talo-2-octulopyranosonic acid (Ko), whereas in a minor fraction (<20%) Ko is replaced by 3-deoxy-D-manno-octulopyranosonic acid (Kdo). The structures of the phosphorylated carbohydrate backbones of these LPS fractions are [see formula in text] with Dha = 3-deoxy-D-lyxo-2-heptulosaric acid, Sug = sugar and is Ko in a major fraction and Kdo in a minor fraction. All sugar residues have the D-configuration and are present in the pyranose form. Mass spectrometry of de-O-acylated LPS revealed the presence of and additional hexose residue in minor amounts, the position and nature of which could not be identified.
Lipopolysaccharide, NMR, structure, Acinetobacter, core-lipid A region
NCBI PubMed ID: 9249012Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Institutions: Department of Chemistry, Carlsberg Laboratory, Copenhagen, Denmark, Division of Medical and Microbiology, Center for Medicine and Biosciences, Research Center Borstel, Germany, Insitute of Chemical Physics, Russian Academy of Sciences, Moscow, Russia, Department of Mass Spectrometry, Bijvoet Center for Biomolecular Research, Universiteit Utrecht, The Netherlands
Methods: NMR-2D, FAB-MS, NMR, analytical methods
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5. Compound ID: 744
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a-D-Glcp-(1-6)-b-D-Glcp-(1-4)-a-D-Glcp-(1-5)-+ P-4)-+
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a-D-Glcp-(1-6)-b-D-Glcp-(1-3)-b-D-Galp-(1-7)-a-Kdop-(2-4)-a-Kop-(2-6)-b-D-GlcpN-(1-6)-a-D-GlcpN-(1-P |
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Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130650,IEDB_135394,IEDB_135608,IEDB_135609,IEDB_136044,IEDB_137340,IEDB_137472,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_190606,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 196
Vinogradov EV, Bock K, Petersen BO, Holst O, Brade H "The structure of the carbohydrate backbone of the lipopolysaccharide from Acinetobacter strain ATCC 17905" -
European Journal of Biochemistry 243(1-2) (1997) 122-127
The structure of the carbohydrate backbone of the lipopolysaccharide from Acinetobacter strain ATCC 17905 was studied. After deacylation of the lipopolysaccharide, a mixture of two compounds (ratio approximately 2:1) was isolated by high-performance anion-exchange chromatography, the structures of which were determined by NMR spectroscopy and electrospray-mass spectrometry as: [see formula in text] [Sug, 3-deoxy-D-manno-2-octulopyranosonic acid (Kdo) in oligosaccharide 1 (major portion) and D-glycero-D-talo-2-octulopyranosonic acid (Ko) in oligosaccharide 2 (minor portion)]. All monosaccharide residues also possess th D-cofiguration and are present in the pyranose form.
Lipopolysaccharide, NMR, Acinetobacter, core-lipid A region
NCBI PubMed ID: 9030730Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Institutions: Department of Chemistry, Carlsberg Laboratory, Copenhagen, Denmark, Division of Biochemical Microbiology, Center for Medicine und Biosciences, Research Center Borstel, Germany
Methods: NMR-2D, NMR, ESI-MS
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6. Compound ID: 776
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b-D-GlcpNAc-(1-3)-+ b-D-Glcp-(1-4)-+ a-Kdop-(2-4)-+
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b-D-Galp-(1-7)-L-gro-a-D-manHepp-(1-7)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-a-Kop-(2--/lipid A/ |
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Structure type: oligomer
Aglycon: lipid A
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130650,IEDB_130670,IEDB_135608,IEDB_135609,IEDB_135813,IEDB_136044,IEDB_137340,IEDB_137472,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_146664,IEDB_151531,IEDB_190606,IEDB_2189047,IEDB_226811,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 201
Vinogradov EV, Lindner B, Kocharova NA, Senchenkova SN, Shashkov AS, Knirel YA, Holst O, Gremyakova TA, Shaikhutdinova RZ, Anisimov AP "The core structure of the lipopolysaccharide from the causative agent o plague, Yersinia pestis" -
Carbohydrate Research 337 (2002) 755-777
The rough-type lipopolysaccharide (LPS) of the plague pathogen, Yersinia pestis, was studied afer mild-acid and strong-alkaline degradations by chemical analyses, NmR spectroscopy and eslectrospray-ionization mass spectrometry, and the following structure of the core region was determined: [see formula in text] where L-a-D-Hep stands for L-glycero-a-D-manno-heptose, Sug1 for either 3-deoxy-a-D-manno-oct-2-ulosonic acid (a-Kdo) ore D-glycero-a-D-talo-oct-2-ulosonic acid (a-Ko), and Sug2 for either b-D-galactose or D-glycero-a-D-manno-heptose. A minority of the LPS molecules lacks GlcNAc
Lipopolysaccharide, core structure, ersinia pestis
NCBI PubMed ID: 11856300Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: knirel@ioc.ac.ru
Institutions: Research Center Borstel, Center for Medicine and Biosciences, Borstel, Germany, N.L. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, State Research Centre for Applied Microgiology, Obolensk, Moscow Region 142279, Russia, N.L. Zelinskty Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
Methods: NMR-2D, NMR, chemical analysis, ESI-MS
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7. Compound ID: 778
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b-D-GlcpNAc-(1-3)-+ b-D-Glcp-(1-4)-+ a-Kdop-(2-4)-+
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D-gro-a-D-manHepp-(1-7)-L-gro-a-D-manHepp-(1-7)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-a-Kop-(2--/lipid A/ |
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Structure type: oligomer
Aglycon: lipid A
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130650,IEDB_130670,IEDB_135608,IEDB_135609,IEDB_135813,IEDB_137340,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_141807,IEDB_142488,IEDB_146664,IEDB_151531,IEDB_2189046,IEDB_2189047,IEDB_226811,IEDB_983931,SB_192
The structure is contained in the following publication(s):
- Article ID: 201
Vinogradov EV, Lindner B, Kocharova NA, Senchenkova SN, Shashkov AS, Knirel YA, Holst O, Gremyakova TA, Shaikhutdinova RZ, Anisimov AP "The core structure of the lipopolysaccharide from the causative agent o plague, Yersinia pestis" -
Carbohydrate Research 337 (2002) 755-777
The rough-type lipopolysaccharide (LPS) of the plague pathogen, Yersinia pestis, was studied afer mild-acid and strong-alkaline degradations by chemical analyses, NmR spectroscopy and eslectrospray-ionization mass spectrometry, and the following structure of the core region was determined: [see formula in text] where L-a-D-Hep stands for L-glycero-a-D-manno-heptose, Sug1 for either 3-deoxy-a-D-manno-oct-2-ulosonic acid (a-Kdo) ore D-glycero-a-D-talo-oct-2-ulosonic acid (a-Ko), and Sug2 for either b-D-galactose or D-glycero-a-D-manno-heptose. A minority of the LPS molecules lacks GlcNAc
Lipopolysaccharide, core structure, ersinia pestis
NCBI PubMed ID: 11856300Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: knirel@ioc.ac.ru
Institutions: Research Center Borstel, Center for Medicine and Biosciences, Borstel, Germany, N.L. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, State Research Centre for Applied Microgiology, Obolensk, Moscow Region 142279, Russia, N.L. Zelinskty Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
Methods: NMR-2D, NMR, chemical analysis, ESI-MS
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8. Compound ID: 815
Structure type: oligomer
Contained glycoepitopes: IEDB_130650,IEDB_135608,IEDB_135609
The structure is contained in the following publication(s):
- Article ID: 219
Wimmer N, Brade H, Kosma P "Synthesis of neoglycoproteins containing D-glycero-D-talo-oct-2-ulopyranosylonic acid (Ko) ligands corresponding to core units from Burkholderia and Acinetobacter lipopolysaccharide" -
Carbohydrate Research 329(3) (2000) 549-560
Glycal esters of Kdo derivatives were converted into 2,3-anhydro intermediates, which were transformed into D-glycero-D-talo-oct-2-ulopyranosylonic acid (Ko), as well as 3-O- and 4-O-p-nitrobenzoyl-Ko derivatives. The exo-allyl orthoester derivative, methyl {5,7,8-tri-O-acetyl-4-O-(4-nitrobenzoyl)-2,3-O-[(1-exo-allyloxy)-ethylidene]-Dglycero-b-D-talo-oct-2-ulopyranos}onate, prepared from the 4-O-pNBz-protected Ko derivative, was elaborated into the a-Ko allyl ketoside, the reducing disaccharide a-Kdop-(2→4)-Ko and the disaccharide a-Kdop-(2→4)-Kop-(2→OAll). Conversely, methyl[4,5,7,8-tetra-O-acetyl-3-O-(4-nitrobenzoyl)-a-D-glycero-D-talo-2-octulopyranosyl bromide] onate [Carbohydr. Res., 244 (1993) 69-84], was coupled with a Kdo acceptor to give the disaccharide a-Kop-(2→4)-Kdop-(2→OAll) after orthoester rearrangement and deprotection. The allyl glycosides were treated with cysteamine and converted into neoglycoproteins. The ligands correspond to inner core units from Acinetobacter haemolyticus and Burkholderia cepacia lipopolysaccharides.
Lipopolysaccharide, synthesis, core, Burkholderia, acid, Acinetobacter, epitope, epitopes, specific, Ko, ligand, Ligands, neoglycoprotein, neoglycoproteins
NCBI PubMed ID: 11128584Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: pkosma@edv2.boku.ac.at
Institutions: Institute of Chemistry, University of Agricultural Sciences, Muthgasse 18, A- 1190 Vienna, Austria, Research Center Borstel, D- 23845 Borstel, Germany
Methods: chemical methods
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9. Compound ID: 816
Structure type: oligomer
Contained glycoepitopes: IEDB_130650,IEDB_135608,IEDB_135609
The structure is contained in the following publication(s):
- Article ID: 219
Wimmer N, Brade H, Kosma P "Synthesis of neoglycoproteins containing D-glycero-D-talo-oct-2-ulopyranosylonic acid (Ko) ligands corresponding to core units from Burkholderia and Acinetobacter lipopolysaccharide" -
Carbohydrate Research 329(3) (2000) 549-560
Glycal esters of Kdo derivatives were converted into 2,3-anhydro intermediates, which were transformed into D-glycero-D-talo-oct-2-ulopyranosylonic acid (Ko), as well as 3-O- and 4-O-p-nitrobenzoyl-Ko derivatives. The exo-allyl orthoester derivative, methyl {5,7,8-tri-O-acetyl-4-O-(4-nitrobenzoyl)-2,3-O-[(1-exo-allyloxy)-ethylidene]-Dglycero-b-D-talo-oct-2-ulopyranos}onate, prepared from the 4-O-pNBz-protected Ko derivative, was elaborated into the a-Ko allyl ketoside, the reducing disaccharide a-Kdop-(2→4)-Ko and the disaccharide a-Kdop-(2→4)-Kop-(2→OAll). Conversely, methyl[4,5,7,8-tetra-O-acetyl-3-O-(4-nitrobenzoyl)-a-D-glycero-D-talo-2-octulopyranosyl bromide] onate [Carbohydr. Res., 244 (1993) 69-84], was coupled with a Kdo acceptor to give the disaccharide a-Kop-(2→4)-Kdop-(2→OAll) after orthoester rearrangement and deprotection. The allyl glycosides were treated with cysteamine and converted into neoglycoproteins. The ligands correspond to inner core units from Acinetobacter haemolyticus and Burkholderia cepacia lipopolysaccharides.
Lipopolysaccharide, synthesis, core, Burkholderia, acid, Acinetobacter, epitope, epitopes, specific, Ko, ligand, Ligands, neoglycoprotein, neoglycoproteins
NCBI PubMed ID: 11128584Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: pkosma@edv2.boku.ac.at
Institutions: Institute of Chemistry, University of Agricultural Sciences, Muthgasse 18, A- 1190 Vienna, Austria, Research Center Borstel, D- 23845 Borstel, Germany
Methods: chemical methods
- Article ID: 904
Kosma P, Reiter A, Zamyatina A, Wimmer N, Glück A, Brade H "Synthesis of inner core antigens related to Chlamydia, Pseudomonas and Acinetobacter LPS" -
Journal of Endotoxin Research 5(3) (1999) 157-163
synthesis, antigen, core, Pseudomonas, Acinetobacter, antigens, Chlamydia, inner core
Journal NLM ID: 9433350Publisher: Maney Publishing
Institutions: Institute of Chemistry, University of Agricultural Sciences, Vienna, Wien, Austria
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10. Compound ID: 3689
Structure type: oligomer
Aglycon: BSA
Contained glycoepitopes: IEDB_130650,IEDB_135608,IEDB_135609
The structure is contained in the following publication(s):
- Article ID: 1389
Brade L, Gronow S, Wimmer N, Kosma P, Brade H "Monoclonal antibodies against 3-deoxy-a-D-manno-oct-2-ulosonic acid (Kdo) and D-glycero-D-talo-oct-2-ulosonic acid (Ko)" -
Journal of Endotoxin Research 8(5) (2002) 358-364
Monoclonal antibodies (mAbs) were obtained after immunization of mice with neoglycoconjugates containing as a ligand the disaccharide Kdo(2→4)Ko or Ko(2→4)Kdo, representing structural elements of the core region of lipopolysaccharides (LPSs) from Acinetobacter haemolyticus and Burkholderia cepacia, respectively. One antibody, S67-9, bound with high specificity to Ko(2→4)Kdo-BSA showing no cross reactivity with Kdo(2→4)Kdo-BSA and the other antigens tested. A second mAb, S68-12, bound preferentially to Kdo(2→4)Ko-BSA but cross reacted with Ko(2→4)Kdo-BSA and Kdo(2→4)Kdo-BSA. A third mAb, S67-27, was found to bind Kdo monosaccharide. Although mAbs S67-9 and S68-12 did not bind to LPS of Burkholderia or Acinetobacter as expected, the mAbs will be useful tools in studying the biosynthesis of LPS containing Ko.
LPS, Pathogenesis, acid, Kdo, antibodies, antibody, monoclonal, monoclonal antibodies, monoclonal antibody, Ko, D-glycero-D-talo-oct-2-ulosonic acid
NCBI PubMed ID: 12537694Journal NLM ID: 9433350Publisher: Maney Publishing
Correspondence: hbrade@fz-borstel.de
Institutions: Research Center Borstel, Center for Medicine and Biosciences, Borstel, Germany
Methods: serological methods
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11. Compound ID: 9674
Structure type: fragment of a bigger structure
Aglycon: lipid A
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130650,IEDB_135608,IEDB_135609
The structure is contained in the following publication(s):
- Article ID: 4049
Knirel YA, Shevelev SD, Perepelov AV "Higher aldulosonic acids: components of bacterial glycans" -
Mendeleev Communications 21(4) (2011) 173-182
Recent data on the natural occurrence, chemistry, and biochemistry of C8 and C9 aldulosonic acids (3-deoxy-d-manno-oct-2-ulosonic acid, sialic acids, N-acyl derivatives of 5,7-diamino-3,5,7,9-tetradeoxynon-2-ulosonic acids, and some others) as well as on the structures and biological significance of bacterial glycans containing these higher acidic monosaccharides are summarized.
structure, Bacterial, glycan, aldulosonic acid, higher acidic monosaccharides, sialic acids
Publication DOI: 10.1016/j.mencom.2011.07.001Journal NLM ID: 9425965Publisher: Moscow: Academy of Sciences of the USSR; Cambridge,UK : Royal Society of Chemistry
Correspondence: knirel@ioc.ac.ru
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
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12. Compound ID: 11761
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a-Kdop-(2-4)-+
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P-6)-+ | P-4)-+
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a-D-3dlyxHepp-ulosaric-(2-6)-b-D-Glcp-(1-4)-a-D-3dlyxHepp-ulosaric-(2-6)-a-D-Glcp-(1-6)-a-D-Glcp-(1-6)-b-D-Glcp-(1-4)-a-D-Glcp-(1-5)-/Variants 0/-b-D-GlcpN-(1-6)-a-D-GlcpN-(1-P
/Variants 0/ is:
a-Kop-(2-6)-
OR (exclusively)
a-Kdop-(2-6)- |
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Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130650,IEDB_130659,IEDB_135394,IEDB_135608,IEDB_135609,IEDB_137340,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_144999,IEDB_146664,IEDB_150908,IEDB_151531,IEDB_241118,IEDB_983931,SB_192
The structure is contained in the following publication(s):
- Article ID: 4716
Pokorny B, Müller-Loennies S, Kosma P "Synthesis of a-D-glucosyl substituted methyl glycosides of 3-deoxy-a-D-manno- and D-glycero-a-D-talo-oct-2-ulosonic acid (Kdo/Ko) corresponding to inner core fragments of Acinetobacter lipopolysaccharide" -
Carbohydrate Research 391 (2014) 66-81
The α-D-glucopyranosyl-(1→5)-substituted methyl glycosides of 3-deoxy-α-D-manno-oct-2-ulosonic acid (Kdo), 3-deoxy-α-D-lyxo-hept-2-ulosonic acid (Kdh), and d-glycero-α-D-talo-oct-2-ulosonic acid (Ko) were prepared using orthogonally protected glycosyl acceptor derivatives via glycosylation with a torsionally disarmed 4,6-O-benzylidene protected trifluoroacetimidate glucosyl donor followed by global deprotection. The related 6-O-phosphoryl-α-D-glucopyranosyl-(1→5)-substituted Kdo and Kdh derivatives were derived from a benzylidene-protected glucosyl intermediate using phosphoramidite and phosphoryl chloride-based phosphorylation steps, respectively. The deprotected disaccharides serve as ligands to study lectin binding of Acinetobacter lipopolysaccharide core oligosaccharides.
Lipopolysaccharide, Acinetobacter, Kdo, oligosaccharide synthesis, Ko
NCBI PubMed ID: 24785390Publication DOI: 10.1016/j.carres.2014.03.004Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: P. Kosma
Institutions: Department of Chemistry, University of Natural Resources and Life Sciences, Muthgasse 18, A-1190 Vienna, Austria, Research Center Borstel, Parkallee 22, D-23845, Germany
Methods: 13C NMR, NMR, TLC, ESI-MS, chemical synthesis, chemical methods, glycosylation, RP-HPLC, ESI-TOF-MS
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