Show legend Show as text

Structure type: oligomer
Aglycon: lipid A
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_115009,IEDB_116046,IEDB_120354,IEDB_123890,IEDB_130650,IEDB_130651,IEDB_136044,IEDB_136906,IEDB_137338,IEDB_137472,IEDB_137777,IEDB_137779,IEDB_138949,IEDB_1391964,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_140624,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_144998,IEDB_146664,IEDB_148488,IEDB_151528,IEDB_152217,IEDB_190606,IEDB_2189047,IEDB_423106,IEDB_742247,IEDB_983931,SB_165,SB_166,SB_167,SB_178,SB_187,SB_192,SB_195,SB_31,SB_6,SB_62,SB_7,SB_88

• Article ID: 244
  Griffin R, Cox AD, Makepeace K, Richards JC, Moxon ER, Hood DW "The role of lex2 in lipopolysaccharide biosynthesis in Haemophilus influenzae strains RM7004 and RM153" - Microbiology (2003) 3165-3175
  

  The locus lex2, comprising lex2A and lex2B, contributes to the phase-variable expression of lipopolysaccharide (LPS) of Haemophilus influenzae and was found to be present in 74 % of strains investigated. lex2A contains 5'-GCAA repeats which vary in number from 4 to 46 copies between strains. The locus was cloned from the serotype b strains RM7004 and RM153 and showed >99 % nucleotide sequence identity between these strains and the published lex2 sequence. Disruption of the lex2B gene in strain RM7004 resulted in truncation of some LPS glycoforms, shown by gel fractionation, with only one glycoform reacting with a digalactoside-specific monoclonal antibody, 4C4, compared with four LPS glycoforms in the more elongated LPS of the parent strain. Mass spectrometry and NMR analyses of LPS from the lex2B mutant revealed loss of the terminal digalactoside as well as the second β-glucose extending from the first heptose of the inner core. The authors conclude that Lex2B is the β-(1-4)-glucosyltransferase that adds the second β-glucose to the first β-glucose as part of the oligosaccharide extension from the first heptose of the LPS of strain RM7004. Investigation of the expression of the lex2 locus indicated that the genes are co-transcribed and that both reading frames are required for addition of this second β-glucose in a phase-variable manner

  Lipopolysaccharide, biosynthesis, Haemophilus influenzae, core, lipopolysaccharide biosynthesis, monoclonal antibodies, inner core

  NCBI PubMed ID: 14600228
  Journal NLM ID: 0376646
  Publisher: Washington, DC: Kluwer Academic/Plenum Publishers
  Correspondence: dhood@molbiol.ox.ac.uk
  Institutions: Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK. Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada K1A 0R6
  
   
  
  Haemophilus influenzae RM7004
  CSDB ID 6866 (all data & tools)

Show legend Show as text

Structure type: oligomer
Aglycon: lipid A
Compound class: LPS
Contained glycoepitopes: IEDB_115009,IEDB_116046,IEDB_120354,IEDB_123890,IEDB_130648,IEDB_130650,IEDB_130651,IEDB_136044,IEDB_136906,IEDB_137338,IEDB_137472,IEDB_137473,IEDB_137777,IEDB_137779,IEDB_138949,IEDB_1391964,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_140624,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_146664,IEDB_151528,IEDB_152217,IEDB_190606,IEDB_2189047,IEDB_241118,IEDB_423106,IEDB_742247,IEDB_983931,SB_165,SB_166,SB_167,SB_178,SB_187,SB_192,SB_195,SB_21,SB_31,SB_6,SB_62,SB_7,SB_88

• Article ID: 964
  Mansson M, Hood DW, Moxon ER, Schweda EK "Structural characterization of a novel branching pattern in the lipopolysaccharide from nontypeable Haemophilus influenzae" - European Journal of Biochemistry 270(14) (2003) 2979-2991
  

  Lipopolysaccharide, Haemophilus, Haemophilus influenzae, structural, characterization, pattern

  Journal NLM ID: 0107600
  Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
  Correspondence: elke.schweda@kfc.ki.se
  Institutions: University College of south Stockholm, Clinical Research Centre, Huddinge, Sweden
  Methods: NMR
  
   
  
  Haemophilus influenzae 981
  CSDB ID 3403 (all data & tools)

Show legend Show as text

Structure type: oligomer
Aglycon: lipid A
Compound class: LPS
Contained glycoepitopes: IEDB_120354,IEDB_123890,IEDB_130650,IEDB_130651,IEDB_136044,IEDB_136906,IEDB_137338,IEDB_137472,IEDB_137777,IEDB_137779,IEDB_138949,IEDB_1391964,IEDB_139428,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_146664,IEDB_151528,IEDB_152217,IEDB_190606,IEDB_2189046,IEDB_2189047,IEDB_423106,IEDB_742247,IEDB_983931,SB_165,SB_166,SB_167,SB_178,SB_187,SB_192,SB_195,SB_31,SB_6,SB_62,SB_7,SB_88

• Article ID: 964
  Mansson M, Hood DW, Moxon ER, Schweda EK "Structural characterization of a novel branching pattern in the lipopolysaccharide from nontypeable Haemophilus influenzae" - European Journal of Biochemistry 270(14) (2003) 2979-2991
  

  Lipopolysaccharide, Haemophilus, Haemophilus influenzae, structural, characterization, pattern

  Journal NLM ID: 0107600
  Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
  Correspondence: elke.schweda@kfc.ki.se
  Institutions: University College of south Stockholm, Clinical Research Centre, Huddinge, Sweden
  Methods: NMR
  
   
  
  Haemophilus influenzae 981
  CSDB ID 3502 (all data & tools)

Show legend Show as text

Structure type: oligomer
Contained glycoepitopes: IEDB_130650,IEDB_130651,IEDB_136044,IEDB_136906,IEDB_137338,IEDB_137340,IEDB_137472,IEDB_137779,IEDB_138949,IEDB_1391964,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_141794,IEDB_141807,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_144998,IEDB_146664,IEDB_148488,IEDB_151528,IEDB_151531,IEDB_152217,IEDB_190606,IEDB_2189047,IEDB_423106,IEDB_742247,IEDB_983931,SB_165,SB_166,SB_167,SB_178,SB_187,SB_192,SB_195,SB_31,SB_6,SB_62,SB_7,SB_88

• Article ID: 974
  Masoud H, Martin A, Thibault P, Moxon ER, Richards JC "Structure of extended lipopolysaccharide glycoforms containing two globotriose units in Haemophilus influenzae serotype b(RM7004)" - Biochemistry 42(15) (2003) 4463-4475
  

  Lipopolysaccharide (LPS) is a major virulence determinant of the human bacterial pathogen Haemophilus influenzae. Structural elucidation of the LPS from H. influenzae type b(RM7004) was achieved by using electrospray ionization mass spectrometry (ESI-MS) and high-field NMR techniques on delipidated LPS and core oligosaccharide samples of LPS. It was found that the organism elaborates a series of related LPS glycoforms having a common inner-core structure, but differing in the number and position of attached hexose residues. LPS glycoforms containing between four and nine hexose residues were structurally characterized. The inner-core element was determined to be L-α-D-Hepp-(1→2)-[PEA→6]-L-α-D-Hepp-(1→3)-[β-D-Glcp-(1→4)]-L-α-D-Hepp-(1→5)-[P→4]-α-KDOp-(2→, a structural feature which has been identified in every H. influenzae strain investigated to date. Two major groups of isomeric glycoforms were characterized in which the terminal Hepp residue of the inner-core element was either substituted at the O-2 position with a β-D-Galp residue or not. The structures of the major LPS glycoforms were found to have oligosaccharide chain extensions from O-3 of the middle Hepp residue. Glycoforms containing five and six hexose residues were most abundant and were shown to carry the tetrasaccharide unit α-D-Galp-(1→4)-β-D-Galp-(1→4)-β-D-Glcp-(1→4)-α-D-Glcp at the O-3 position of the middle heptose. This tetrasaccharide displays the globoside trisaccharide (globotriose) as a terminal epitope, a structure that is found on many human cells (P(k) blood group antigen) and which is thought to be an important virulence determinant for H. influenzae. LPS glycoforms were characterized that had further chain extension from the β-D-Glcp-(1→ residue of the proximal Hepp. In the fully extended LPS (Hex9/Hex8' glycoforms), both the proximal and middle heptose residues carried tetrasaccharide chains displaying terminal globotriose epitopes. In addition, the LPS was found to carry phosphorylcholine and O-acetyl groups.

  Lipopolysaccharide, NMR, Haemophilus influenzae, Haemophilus influenzae type b

  NCBI PubMed ID: 12693942
  Publication DOI: 10.1021/bi026632a
  Journal NLM ID: 0370623
  Publisher: American Chemical Society
  Correspondence: Jim.Richards@nrc.ca
  Institutions: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Canada, Department of Biological Sciences, Faculty of Science, University of Jordan, Amman, Jordan, and Molecular Infectious Diseases Group, Department of Paediatrics, Institute for Molecular Medicine, John Radcliffe Hospital, Headington, Oxford 0X3 3DU, United Kingdom
  Methods: NMR
  
   
  
  Haemophilus influenzae b (RM7004)
  CSDB ID 3414 (all data & tools)

Show legend Show as text

Structure type: oligomer
Contained glycoepitopes: IEDB_130650,IEDB_130651,IEDB_136044,IEDB_136906,IEDB_137338,IEDB_137340,IEDB_137472,IEDB_137779,IEDB_138949,IEDB_1391964,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_141794,IEDB_141807,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_152217,IEDB_190606,IEDB_2189047,IEDB_423106,IEDB_742247,IEDB_983931,SB_165,SB_166,SB_167,SB_178,SB_187,SB_192,SB_195,SB_31,SB_6,SB_62,SB_7,SB_88

• Article ID: 974
  Masoud H, Martin A, Thibault P, Moxon ER, Richards JC "Structure of extended lipopolysaccharide glycoforms containing two globotriose units in Haemophilus influenzae serotype b(RM7004)" - Biochemistry 42(15) (2003) 4463-4475
  

  Lipopolysaccharide (LPS) is a major virulence determinant of the human bacterial pathogen Haemophilus influenzae. Structural elucidation of the LPS from H. influenzae type b(RM7004) was achieved by using electrospray ionization mass spectrometry (ESI-MS) and high-field NMR techniques on delipidated LPS and core oligosaccharide samples of LPS. It was found that the organism elaborates a series of related LPS glycoforms having a common inner-core structure, but differing in the number and position of attached hexose residues. LPS glycoforms containing between four and nine hexose residues were structurally characterized. The inner-core element was determined to be L-α-D-Hepp-(1→2)-[PEA→6]-L-α-D-Hepp-(1→3)-[β-D-Glcp-(1→4)]-L-α-D-Hepp-(1→5)-[P→4]-α-KDOp-(2→, a structural feature which has been identified in every H. influenzae strain investigated to date. Two major groups of isomeric glycoforms were characterized in which the terminal Hepp residue of the inner-core element was either substituted at the O-2 position with a β-D-Galp residue or not. The structures of the major LPS glycoforms were found to have oligosaccharide chain extensions from O-3 of the middle Hepp residue. Glycoforms containing five and six hexose residues were most abundant and were shown to carry the tetrasaccharide unit α-D-Galp-(1→4)-β-D-Galp-(1→4)-β-D-Glcp-(1→4)-α-D-Glcp at the O-3 position of the middle heptose. This tetrasaccharide displays the globoside trisaccharide (globotriose) as a terminal epitope, a structure that is found on many human cells (P(k) blood group antigen) and which is thought to be an important virulence determinant for H. influenzae. LPS glycoforms were characterized that had further chain extension from the β-D-Glcp-(1→ residue of the proximal Hepp. In the fully extended LPS (Hex9/Hex8' glycoforms), both the proximal and middle heptose residues carried tetrasaccharide chains displaying terminal globotriose epitopes. In addition, the LPS was found to carry phosphorylcholine and O-acetyl groups.

  Lipopolysaccharide, NMR, Haemophilus influenzae, Haemophilus influenzae type b

  NCBI PubMed ID: 12693942
  Publication DOI: 10.1021/bi026632a
  Journal NLM ID: 0370623
  Publisher: American Chemical Society
  Correspondence: Jim.Richards@nrc.ca
  Institutions: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Canada, Department of Biological Sciences, Faculty of Science, University of Jordan, Amman, Jordan, and Molecular Infectious Diseases Group, Department of Paediatrics, Institute for Molecular Medicine, John Radcliffe Hospital, Headington, Oxford 0X3 3DU, United Kingdom
  Methods: NMR
  
   
  
  Haemophilus influenzae b (RM7004)
  CSDB ID 3562 (all data & tools)

Show legend Show as text

Structure type: oligomer
Aglycon: lipid A
Contained glycoepitopes: IEDB_120354,IEDB_123890,IEDB_130650,IEDB_130651,IEDB_136044,IEDB_136906,IEDB_137338,IEDB_137472,IEDB_137777,IEDB_137779,IEDB_138949,IEDB_1391964,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_144998,IEDB_146664,IEDB_148488,IEDB_151528,IEDB_152217,IEDB_190606,IEDB_2189047,IEDB_423106,IEDB_742247,IEDB_983931,SB_165,SB_166,SB_167,SB_178,SB_187,SB_192,SB_195,SB_31,SB_6,SB_62,SB_7,SB_88

• Article ID: 974
  Masoud H, Martin A, Thibault P, Moxon ER, Richards JC "Structure of extended lipopolysaccharide glycoforms containing two globotriose units in Haemophilus influenzae serotype b(RM7004)" - Biochemistry 42(15) (2003) 4463-4475
  

  Lipopolysaccharide (LPS) is a major virulence determinant of the human bacterial pathogen Haemophilus influenzae. Structural elucidation of the LPS from H. influenzae type b(RM7004) was achieved by using electrospray ionization mass spectrometry (ESI-MS) and high-field NMR techniques on delipidated LPS and core oligosaccharide samples of LPS. It was found that the organism elaborates a series of related LPS glycoforms having a common inner-core structure, but differing in the number and position of attached hexose residues. LPS glycoforms containing between four and nine hexose residues were structurally characterized. The inner-core element was determined to be L-α-D-Hepp-(1→2)-[PEA→6]-L-α-D-Hepp-(1→3)-[β-D-Glcp-(1→4)]-L-α-D-Hepp-(1→5)-[P→4]-α-KDOp-(2→, a structural feature which has been identified in every H. influenzae strain investigated to date. Two major groups of isomeric glycoforms were characterized in which the terminal Hepp residue of the inner-core element was either substituted at the O-2 position with a β-D-Galp residue or not. The structures of the major LPS glycoforms were found to have oligosaccharide chain extensions from O-3 of the middle Hepp residue. Glycoforms containing five and six hexose residues were most abundant and were shown to carry the tetrasaccharide unit α-D-Galp-(1→4)-β-D-Galp-(1→4)-β-D-Glcp-(1→4)-α-D-Glcp at the O-3 position of the middle heptose. This tetrasaccharide displays the globoside trisaccharide (globotriose) as a terminal epitope, a structure that is found on many human cells (P(k) blood group antigen) and which is thought to be an important virulence determinant for H. influenzae. LPS glycoforms were characterized that had further chain extension from the β-D-Glcp-(1→ residue of the proximal Hepp. In the fully extended LPS (Hex9/Hex8' glycoforms), both the proximal and middle heptose residues carried tetrasaccharide chains displaying terminal globotriose epitopes. In addition, the LPS was found to carry phosphorylcholine and O-acetyl groups.

  Lipopolysaccharide, NMR, Haemophilus influenzae, Haemophilus influenzae type b

  NCBI PubMed ID: 12693942
  Publication DOI: 10.1021/bi026632a
  Journal NLM ID: 0370623
  Publisher: American Chemical Society
  Correspondence: Jim.Richards@nrc.ca
  Institutions: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Canada, Department of Biological Sciences, Faculty of Science, University of Jordan, Amman, Jordan, and Molecular Infectious Diseases Group, Department of Paediatrics, Institute for Molecular Medicine, John Radcliffe Hospital, Headington, Oxford 0X3 3DU, United Kingdom
  Methods: NMR
  
   
  
  Haemophilus influenzae b (RM7004)
  CSDB ID 3573 (all data & tools)

Show legend Show as text

Structure type: oligomer
Aglycon: lipid A
Contained glycoepitopes: IEDB_120354,IEDB_123890,IEDB_130650,IEDB_130651,IEDB_136044,IEDB_136906,IEDB_137338,IEDB_137472,IEDB_137777,IEDB_137779,IEDB_138949,IEDB_1391964,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_152217,IEDB_190606,IEDB_2189047,IEDB_423106,IEDB_742247,IEDB_983931,SB_165,SB_166,SB_167,SB_178,SB_187,SB_192,SB_195,SB_31,SB_6,SB_62,SB_7,SB_88

• Article ID: 974
  Masoud H, Martin A, Thibault P, Moxon ER, Richards JC "Structure of extended lipopolysaccharide glycoforms containing two globotriose units in Haemophilus influenzae serotype b(RM7004)" - Biochemistry 42(15) (2003) 4463-4475
  

  Lipopolysaccharide (LPS) is a major virulence determinant of the human bacterial pathogen Haemophilus influenzae. Structural elucidation of the LPS from H. influenzae type b(RM7004) was achieved by using electrospray ionization mass spectrometry (ESI-MS) and high-field NMR techniques on delipidated LPS and core oligosaccharide samples of LPS. It was found that the organism elaborates a series of related LPS glycoforms having a common inner-core structure, but differing in the number and position of attached hexose residues. LPS glycoforms containing between four and nine hexose residues were structurally characterized. The inner-core element was determined to be L-α-D-Hepp-(1→2)-[PEA→6]-L-α-D-Hepp-(1→3)-[β-D-Glcp-(1→4)]-L-α-D-Hepp-(1→5)-[P→4]-α-KDOp-(2→, a structural feature which has been identified in every H. influenzae strain investigated to date. Two major groups of isomeric glycoforms were characterized in which the terminal Hepp residue of the inner-core element was either substituted at the O-2 position with a β-D-Galp residue or not. The structures of the major LPS glycoforms were found to have oligosaccharide chain extensions from O-3 of the middle Hepp residue. Glycoforms containing five and six hexose residues were most abundant and were shown to carry the tetrasaccharide unit α-D-Galp-(1→4)-β-D-Galp-(1→4)-β-D-Glcp-(1→4)-α-D-Glcp at the O-3 position of the middle heptose. This tetrasaccharide displays the globoside trisaccharide (globotriose) as a terminal epitope, a structure that is found on many human cells (P(k) blood group antigen) and which is thought to be an important virulence determinant for H. influenzae. LPS glycoforms were characterized that had further chain extension from the β-D-Glcp-(1→ residue of the proximal Hepp. In the fully extended LPS (Hex9/Hex8' glycoforms), both the proximal and middle heptose residues carried tetrasaccharide chains displaying terminal globotriose epitopes. In addition, the LPS was found to carry phosphorylcholine and O-acetyl groups.

  Lipopolysaccharide, NMR, Haemophilus influenzae, Haemophilus influenzae type b

  NCBI PubMed ID: 12693942
  Publication DOI: 10.1021/bi026632a
  Journal NLM ID: 0370623
  Publisher: American Chemical Society
  Correspondence: Jim.Richards@nrc.ca
  Institutions: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Canada, Department of Biological Sciences, Faculty of Science, University of Jordan, Amman, Jordan, and Molecular Infectious Diseases Group, Department of Paediatrics, Institute for Molecular Medicine, John Radcliffe Hospital, Headington, Oxford 0X3 3DU, United Kingdom
  Methods: NMR
  
   
  
  Haemophilus influenzae b (RM7004)
  CSDB ID 3594 (all data & tools)

Show legend Show as text

Structure type: oligomer
Aglycon: lipid A
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_115009,IEDB_116046,IEDB_120354,IEDB_123890,IEDB_130650,IEDB_130651,IEDB_136044,IEDB_136906,IEDB_137338,IEDB_137472,IEDB_137777,IEDB_137779,IEDB_138949,IEDB_1391964,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_140624,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_144998,IEDB_146664,IEDB_148488,IEDB_151528,IEDB_152217,IEDB_190606,IEDB_2189047,IEDB_423106,IEDB_742247,IEDB_983931,SB_165,SB_166,SB_167,SB_178,SB_187,SB_192,SB_195,SB_31,SB_6,SB_62,SB_7,SB_88

• Article ID: 1514
  Griffin R, Cox AD, Makepeace K, Richards JC, Moxon ER, Hood DW "Elucidation of the Monoclonal Antibody 5G8-Reactive, Virulence-Associated Lipopolysaccharide Epitope of Haemophilus influenzae and Its Role in Bacterial Resistance to Complement-Mediated Killing" - Infection and Immunity 73(4) (2005) 2213-2221
  

  The phase-variable locus lex2 is required for expression of a Haemophilus influenzae lipopolysaccharide (LPS) epitope of previously unknown structure. This epitope, which is reactive with monoclonal antibody (MAb) 5G8, has been associated with virulence of type b strains. When strain RM118 (from the same source as strain Rd), in which the lex2 locus and MAb 5G8 reactivity are absent, was transformed with lex2 DNA, transformants that were reactive with MAb 5G8 were obtained. Surprisingly, the 5G8 reactivity of these transformants was phase variable, although the lex2 locus lacked tetrameric repeats and was constitutively expressed. This phase variation was shown to be the result of phase-variable expression of phosphorylcholine (PCho) such that MAb 5G8 reacted only in the absence of PCho. Structural analysis showed that, compared to RM118, the lex2 transformant had acquired a tetrasaccharide, Gal-α1,4-Gal-β1,4-Glc-β1,4-Glc-β1,4, linked to the proximal heptose (HepI). A terminal GalNAc was detected in a minority of glycoforms. LPS derived from a mutant of RM7004, a virulent type b strain which naturally expresses lex2 and has LPS containing the same tetrasaccharide linked to HepI as the sole oligosaccharide extension from the inner core, confirmed that GalNAc is not a part of the MAb 5G8-reactive epitope. Thus, MAb 5G8 specifically binds to the structure Gal-α1,4-Gal-β1,4-Glc-β1,4-Glc-β attached via a 1,4 linkage to HepI of H. influenzae LPS, and we show that the ability to synthesize this novel tetrasaccharide was associated with enhanced bacterial resistance to complement-mediated killing

  Lipopolysaccharide, Haemophilus, Haemophilus influenzae, LPS, oligosaccharide, structure, core, heptose, tetrasaccharide, Phase variation, expression, Bacterial, microbiology, phase, role, DNA, strain, structural, terminal, variation, virulence, analysis, structural analysis, linked, molecular, locus, antibodies, antibody, epitope, monoclonal, monoclonal antibodies, monoclonal antibody, type, enhanced, infection, level, MAb, mutant, linkage, inner core, elucidation, resistance, ability, killing, phosphorylcholine, virulent, variable, reactivity, source, absence

  NCBI PubMed ID: 15784565
  Journal NLM ID: 0246127
  Publisher: American Society for Microbiology
  Correspondence: r.griffin@imperial.ac.uk
  Institutions: Centre for Molecular Microbiology and Infection, Level 3, Flowers Building, Imperial College, London SW7 2AZ, United Kingdom
  Methods: serological methods, genetic methods
  
   
  
  Haemophilus influenzae RM7004
  CSDB ID 10048 (all data & tools)

Show legend Show as text

Structure type: oligomer
Aglycon: lipid A
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130650,IEDB_130651,IEDB_136044,IEDB_136906,IEDB_137338,IEDB_137472,IEDB_137777,IEDB_137779,IEDB_138949,IEDB_1391964,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_144998,IEDB_146664,IEDB_148488,IEDB_151528,IEDB_152217,IEDB_190606,IEDB_2189047,IEDB_423106,IEDB_742247,IEDB_983931,SB_165,SB_166,SB_167,SB_178,SB_187,SB_192,SB_195,SB_31,SB_6,SB_62,SB_7,SB_88

• Article ID: 1614
  Griffin R, Bayliss CD, Herbert MA, Cox AD, Makepeace K, Richards JC, Hood DW, Moxon ER "Digalactoside expression in the lipopolysaccharide of Haemophilus influenzae and its role in intravascular survival" - Infection and Immunity 73(10) (2005) 7022-7026
  

  Digalactoside (galα-1-4 galβ) structures of the lipopolysaccharide (LPS) of Haemophilus influenzae are implicated in virulence. A confounding factor is that tetranucleotide repeats within the lic2A, lgtC, and lex2 genes mediate phase-variable expression of the digalactosides. By deleting these repeats, we constructed recombinant strains of RM153 constitutively expressing either one or two LPS digalactosides. Expression of two digalactosides, rather than one, was associated with increased virulence of H. influenzae in vivo.

  Lipopolysaccharide, Haemophilus, Haemophilus influenzae, LPS, structure, disease, expression, gene, role, strain, virulence, group, molecular, factor, infectious disease, medicine, Infectious, recombinant, in vivo, survival

  NCBI PubMed ID: 16177385
  Journal NLM ID: 0246127
  Publisher: American Society for Microbiology
  Correspondence: r.griffin@imperial.ac.uk
  Institutions: Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, UK
  Methods: genetic methods
  
   
  
  Haemophilus influenzae b RM7004
  CSDB ID 10544 (all data & tools)

Show legend Show as text

Structure type: fragment of a bigger structure
Aglycon: (1->4) core Hep I
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130648,IEDB_130651,IEDB_136044,IEDB_136906,IEDB_137338,IEDB_137472,IEDB_137473,IEDB_1391964,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_146664,IEDB_151528,IEDB_152217,IEDB_190606,IEDB_423106,IEDB_742247,IEDB_983931,SB_165,SB_166,SB_167,SB_178,SB_187,SB_192,SB_195,SB_21,SB_31,SB_6,SB_62,SB_7,SB_88

• Article ID: 3345
  Schweda EK, Richards JC, Hood DW, Moxon ER "Expression and structural diversity of the lipopolysaccharide of Haemophilus influenzae: implication in virulence" - International Journal of Medical Microbiology 297(5) (2007) 297-306
  

  Lipopolysaccharide (LPS) is a major virulence determinant of the human bacterial pathogen Haemophilus influenzae. A characteristic feature of H. influenzae LPS is the extensive intra- and inter-strain heterogeneity of glycoform structure which is key to the role of the molecule in both commensal and disease-causing behaviour of the bacterium. Through the combination of genetics and detailed structural analyses, H. influenzae is an exemplar Gram-negative bacterium for which now the most extensive and detailed LPS structural data and functional correlates are available. LPS from H. influenzae consists of a conserved glucose-substituted triheptosyl inner-core moiety L-α-D-Hepp-(1→2)-[PEtn→6]-L-α-D-Hepp-(1→3)-[β-D-Glcp-(1→4)]-L-α-D-Hepp linked to lipid A via Kdo 4-phosphate. The inner-core unit provides the template for attachment of oligosaccharide- and non-carbohydrate substituents. Here, the structure, genetics and expression of LPS glycoforms in the outer core are reviewed as well as their implication on virulence

  Lipopolysaccharide, Haemophilus influenzae, molecular mimicry, Phosphocholine, sialic acid, Digalactoside

  NCBI PubMed ID: 17452015
  Publication DOI: 10.1016/j.ijmm.2007.03.007
  Journal NLM ID: 100898849
  Publisher: Jena, Germany: Urban & Fischer Verlag
  Correspondence: elke.schweda@crc.ki.se
  Institutions: Clinical Research Centre, Karolinska Institutet and University College of South Stockholm, NOVUM, S-141 86 Huddinge, Sweden
  
   
  
  Haemophilus influenzae
  CSDB ID 21824 (all data & tools)

Show legend Show as text

Structure type: fragment of a bigger structure
Aglycon: (1->4) core Hep I
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130648,IEDB_130651,IEDB_136044,IEDB_136906,IEDB_137338,IEDB_137472,IEDB_137473,IEDB_1391964,IEDB_139428,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_146664,IEDB_151528,IEDB_152217,IEDB_190606,IEDB_2189046,IEDB_423106,IEDB_742247,IEDB_983931,SB_165,SB_166,SB_167,SB_178,SB_187,SB_192,SB_195,SB_21,SB_31,SB_6,SB_62,SB_7,SB_88

• Article ID: 3345
  Schweda EK, Richards JC, Hood DW, Moxon ER "Expression and structural diversity of the lipopolysaccharide of Haemophilus influenzae: implication in virulence" - International Journal of Medical Microbiology 297(5) (2007) 297-306
  

  Lipopolysaccharide (LPS) is a major virulence determinant of the human bacterial pathogen Haemophilus influenzae. A characteristic feature of H. influenzae LPS is the extensive intra- and inter-strain heterogeneity of glycoform structure which is key to the role of the molecule in both commensal and disease-causing behaviour of the bacterium. Through the combination of genetics and detailed structural analyses, H. influenzae is an exemplar Gram-negative bacterium for which now the most extensive and detailed LPS structural data and functional correlates are available. LPS from H. influenzae consists of a conserved glucose-substituted triheptosyl inner-core moiety L-α-D-Hepp-(1→2)-[PEtn→6]-L-α-D-Hepp-(1→3)-[β-D-Glcp-(1→4)]-L-α-D-Hepp linked to lipid A via Kdo 4-phosphate. The inner-core unit provides the template for attachment of oligosaccharide- and non-carbohydrate substituents. Here, the structure, genetics and expression of LPS glycoforms in the outer core are reviewed as well as their implication on virulence

  Lipopolysaccharide, Haemophilus influenzae, molecular mimicry, Phosphocholine, sialic acid, Digalactoside

  NCBI PubMed ID: 17452015
  Publication DOI: 10.1016/j.ijmm.2007.03.007
  Journal NLM ID: 100898849
  Publisher: Jena, Germany: Urban & Fischer Verlag
  Correspondence: elke.schweda@crc.ki.se
  Institutions: Clinical Research Centre, Karolinska Institutet and University College of South Stockholm, NOVUM, S-141 86 Huddinge, Sweden
  
   
  
  Haemophilus influenzae
  CSDB ID 21825 (all data & tools)

Show legend Show as text

Structure type: oligomer
Trivial name: disialylated glycoform
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_115009,IEDB_116046,IEDB_120354,IEDB_123890,IEDB_130648,IEDB_130651,IEDB_130676,IEDB_130679,IEDB_136044,IEDB_136794,IEDB_136906,IEDB_137338,IEDB_137472,IEDB_137473,IEDB_137779,IEDB_1391964,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_140624,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_150937,IEDB_151528,IEDB_152217,IEDB_153198,IEDB_153199,IEDB_190606,IEDB_2189047,IEDB_241118,IEDB_423106,IEDB_742247,IEDB_858580,IEDB_983931,SB_116,SB_165,SB_166,SB_167,SB_170,SB_171,SB_172,SB_178,SB_187,SB_192,SB_195,SB_21,SB_31,SB_35,SB_37,SB_39,SB_42,SB_6,SB_61,SB_62,SB_68,SB_7,SB_76,SB_84,SB_88

• Article ID: 4125
  Twelkmeyer B, Burstrom PK, Li J, Richard ME, Hood DW, Schweda EK "Expression of a new disialyllacto structure in the lipopolysaccharide of non-typeable Haemophilus influenzae" - Carbohydrate Research 346(13) (2011) 1885-1897
  

  The structure of lipopolysaccharide (LPS) expressed by non-typeable Haemophilus influenzae (NTHi) strains 1008 and 1247 has been investigated by mass spectrometry and NMR analyses on O-deacylated LPS and core oligosaccharide material. Both strains express the conserved triheptosyl inner core, [L-α-D-Hepp-(1→2)-[PEtn→6]-L-α-D-Hepp-(1→3)-L-α-D-Hepp-(1→5)-[PPEtn→4]-α-Kdo-(2→6)-Lipid A] with PCho→6)-β-D-Glcp (GlcI) substituting the proximal heptose (HepI) at O-4. Strain 1247 expresses the common structural motifs of H. influenzae; globotetraose [β-D-GalpNAc-(1→3)-α-D-Galp-(1→4)-β-D-Galp-(1→4)-β-D-Glcp-(1→] and its truncated versions globoside [α-D-Galp-(1→4)-β-D-Galp-(1→4)-β-D-Glcp-(1→] and lactose [β-D-Galp-(1→4)-β-D-Glcp-(1→] linked to the terminal heptose of the inner core and GlcI. A genetically distinct NTHi strain, 1008, expresses identical structures to strain 1247 with the exception that it lacks GalNAc. A lpsA mutant of strain 1247 expressed LPS of reduced complexity that facilitated unambiguous structural determination of the oligosaccharide from HepI. By CE-ESI-MS/MS we identified disialylated glycoforms indicating disialyllactose [α-Neu5Ac-(2→8)-α-Neu5Ac-(2→3)-β-D-Gal-(1→4)-β-D-Glcp-(1→] as an extension from GlcI which is a novel finding for NTHi LPS.

  Lipopolysaccharide, Haemophilus influenzae, otitis media, Digalactoside, disialyllactoside

  NCBI PubMed ID: 21683945
  Publication DOI: 10.1016/j.carres.2011.05.020
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: E.K.H. Schweda
  Institutions: Karolinska Institutet, Clinical Research Centre, Huddinge, Sweden
  Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, HF solvolysis, ESI-MS, mild acid hydrolysis, de-O-acylation with hydrazine, composition analysis, NMR-1D, alkaline deacylation, HPAEC-PAD, CE-ESI-MS/MS, CE-ESI-MS, LC-ESI-MS
  
   
  
  Haemophilus influenzae NTHi 1247lpsA
  CSDB ID 26373 (all data & tools)

Show legend Show as text

Structure type: oligomer
Trivial name: HexNAcHex4 glycoform
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_115009,IEDB_116046,IEDB_120354,IEDB_123890,IEDB_130648,IEDB_130651,IEDB_136044,IEDB_136906,IEDB_137338,IEDB_137472,IEDB_137473,IEDB_137779,IEDB_1391964,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_140624,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_146664,IEDB_151528,IEDB_152217,IEDB_190606,IEDB_2189047,IEDB_241118,IEDB_423106,IEDB_742247,IEDB_983931,SB_165,SB_166,SB_167,SB_178,SB_187,SB_192,SB_195,SB_21,SB_31,SB_6,SB_61,SB_62,SB_7,SB_88

• Article ID: 4125
  Twelkmeyer B, Burstrom PK, Li J, Richard ME, Hood DW, Schweda EK "Expression of a new disialyllacto structure in the lipopolysaccharide of non-typeable Haemophilus influenzae" - Carbohydrate Research 346(13) (2011) 1885-1897
  

  The structure of lipopolysaccharide (LPS) expressed by non-typeable Haemophilus influenzae (NTHi) strains 1008 and 1247 has been investigated by mass spectrometry and NMR analyses on O-deacylated LPS and core oligosaccharide material. Both strains express the conserved triheptosyl inner core, [L-α-D-Hepp-(1→2)-[PEtn→6]-L-α-D-Hepp-(1→3)-L-α-D-Hepp-(1→5)-[PPEtn→4]-α-Kdo-(2→6)-Lipid A] with PCho→6)-β-D-Glcp (GlcI) substituting the proximal heptose (HepI) at O-4. Strain 1247 expresses the common structural motifs of H. influenzae; globotetraose [β-D-GalpNAc-(1→3)-α-D-Galp-(1→4)-β-D-Galp-(1→4)-β-D-Glcp-(1→] and its truncated versions globoside [α-D-Galp-(1→4)-β-D-Galp-(1→4)-β-D-Glcp-(1→] and lactose [β-D-Galp-(1→4)-β-D-Glcp-(1→] linked to the terminal heptose of the inner core and GlcI. A genetically distinct NTHi strain, 1008, expresses identical structures to strain 1247 with the exception that it lacks GalNAc. A lpsA mutant of strain 1247 expressed LPS of reduced complexity that facilitated unambiguous structural determination of the oligosaccharide from HepI. By CE-ESI-MS/MS we identified disialylated glycoforms indicating disialyllactose [α-Neu5Ac-(2→8)-α-Neu5Ac-(2→3)-β-D-Gal-(1→4)-β-D-Glcp-(1→] as an extension from GlcI which is a novel finding for NTHi LPS.

  Lipopolysaccharide, Haemophilus influenzae, otitis media, Digalactoside, disialyllactoside

  NCBI PubMed ID: 21683945
  Publication DOI: 10.1016/j.carres.2011.05.020
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: E.K.H. Schweda
  Institutions: Karolinska Institutet, Clinical Research Centre, Huddinge, Sweden
  Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, HF solvolysis, ESI-MS, mild acid hydrolysis, de-O-acylation with hydrazine, composition analysis, NMR-1D, alkaline deacylation, HPAEC-PAD, CE-ESI-MS/MS, CE-ESI-MS, LC-ESI-MS
  
   
  
  Haemophilus influenzae NTHi 1247lpsA
  CSDB ID 26977 (all data & tools)

Show legend Show as text

Structure type: oligomer
Aglycon: lipid A
Trivial name: HexNAcHex7 glycoform
Compound class: LPS
Contained glycoepitopes: IEDB_115009,IEDB_116046,IEDB_120354,IEDB_123890,IEDB_130648,IEDB_130650,IEDB_130651,IEDB_136044,IEDB_136906,IEDB_137338,IEDB_137472,IEDB_137473,IEDB_137777,IEDB_137779,IEDB_138949,IEDB_1391964,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_140624,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_146664,IEDB_151528,IEDB_152217,IEDB_190606,IEDB_2189047,IEDB_241118,IEDB_423106,IEDB_742247,IEDB_983931,SB_165,SB_166,SB_167,SB_178,SB_187,SB_192,SB_195,SB_21,SB_31,SB_6,SB_62,SB_7,SB_88

• Article ID: 4125
  Twelkmeyer B, Burstrom PK, Li J, Richard ME, Hood DW, Schweda EK "Expression of a new disialyllacto structure in the lipopolysaccharide of non-typeable Haemophilus influenzae" - Carbohydrate Research 346(13) (2011) 1885-1897
  

  The structure of lipopolysaccharide (LPS) expressed by non-typeable Haemophilus influenzae (NTHi) strains 1008 and 1247 has been investigated by mass spectrometry and NMR analyses on O-deacylated LPS and core oligosaccharide material. Both strains express the conserved triheptosyl inner core, [L-α-D-Hepp-(1→2)-[PEtn→6]-L-α-D-Hepp-(1→3)-L-α-D-Hepp-(1→5)-[PPEtn→4]-α-Kdo-(2→6)-Lipid A] with PCho→6)-β-D-Glcp (GlcI) substituting the proximal heptose (HepI) at O-4. Strain 1247 expresses the common structural motifs of H. influenzae; globotetraose [β-D-GalpNAc-(1→3)-α-D-Galp-(1→4)-β-D-Galp-(1→4)-β-D-Glcp-(1→] and its truncated versions globoside [α-D-Galp-(1→4)-β-D-Galp-(1→4)-β-D-Glcp-(1→] and lactose [β-D-Galp-(1→4)-β-D-Glcp-(1→] linked to the terminal heptose of the inner core and GlcI. A genetically distinct NTHi strain, 1008, expresses identical structures to strain 1247 with the exception that it lacks GalNAc. A lpsA mutant of strain 1247 expressed LPS of reduced complexity that facilitated unambiguous structural determination of the oligosaccharide from HepI. By CE-ESI-MS/MS we identified disialylated glycoforms indicating disialyllactose [α-Neu5Ac-(2→8)-α-Neu5Ac-(2→3)-β-D-Gal-(1→4)-β-D-Glcp-(1→] as an extension from GlcI which is a novel finding for NTHi LPS.

  Lipopolysaccharide, Haemophilus influenzae, otitis media, Digalactoside, disialyllactoside

  NCBI PubMed ID: 21683945
  Publication DOI: 10.1016/j.carres.2011.05.020
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: E.K.H. Schweda
  Institutions: Karolinska Institutet, Clinical Research Centre, Huddinge, Sweden
  Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, HF solvolysis, ESI-MS, mild acid hydrolysis, de-O-acylation with hydrazine, composition analysis, NMR-1D, alkaline deacylation, HPAEC-PAD, CE-ESI-MS/MS, CE-ESI-MS, LC-ESI-MS
  
   
  
  Haemophilus influenzae NTHi 1247
  CSDB ID 26993 (all data & tools)

Show legend Show as text

Structure type: oligomer
Aglycon: lipid A
Trivial name: Hex7 glycoform
Compound class: LPS
Contained glycoepitopes: IEDB_115009,IEDB_116046,IEDB_120354,IEDB_123890,IEDB_130650,IEDB_130651,IEDB_136044,IEDB_136906,IEDB_137338,IEDB_137472,IEDB_137777,IEDB_137779,IEDB_138949,IEDB_1391964,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_140624,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_146664,IEDB_151528,IEDB_152217,IEDB_190606,IEDB_2189047,IEDB_241118,IEDB_423106,IEDB_742247,IEDB_983931,SB_165,SB_166,SB_167,SB_178,SB_187,SB_192,SB_195,SB_31,SB_6,SB_62,SB_7,SB_88

• Article ID: 4125
  Twelkmeyer B, Burstrom PK, Li J, Richard ME, Hood DW, Schweda EK "Expression of a new disialyllacto structure in the lipopolysaccharide of non-typeable Haemophilus influenzae" - Carbohydrate Research 346(13) (2011) 1885-1897
  

  The structure of lipopolysaccharide (LPS) expressed by non-typeable Haemophilus influenzae (NTHi) strains 1008 and 1247 has been investigated by mass spectrometry and NMR analyses on O-deacylated LPS and core oligosaccharide material. Both strains express the conserved triheptosyl inner core, [L-α-D-Hepp-(1→2)-[PEtn→6]-L-α-D-Hepp-(1→3)-L-α-D-Hepp-(1→5)-[PPEtn→4]-α-Kdo-(2→6)-Lipid A] with PCho→6)-β-D-Glcp (GlcI) substituting the proximal heptose (HepI) at O-4. Strain 1247 expresses the common structural motifs of H. influenzae; globotetraose [β-D-GalpNAc-(1→3)-α-D-Galp-(1→4)-β-D-Galp-(1→4)-β-D-Glcp-(1→] and its truncated versions globoside [α-D-Galp-(1→4)-β-D-Galp-(1→4)-β-D-Glcp-(1→] and lactose [β-D-Galp-(1→4)-β-D-Glcp-(1→] linked to the terminal heptose of the inner core and GlcI. A genetically distinct NTHi strain, 1008, expresses identical structures to strain 1247 with the exception that it lacks GalNAc. A lpsA mutant of strain 1247 expressed LPS of reduced complexity that facilitated unambiguous structural determination of the oligosaccharide from HepI. By CE-ESI-MS/MS we identified disialylated glycoforms indicating disialyllactose [α-Neu5Ac-(2→8)-α-Neu5Ac-(2→3)-β-D-Gal-(1→4)-β-D-Glcp-(1→] as an extension from GlcI which is a novel finding for NTHi LPS.

  Lipopolysaccharide, Haemophilus influenzae, otitis media, Digalactoside, disialyllactoside

  NCBI PubMed ID: 21683945
  Publication DOI: 10.1016/j.carres.2011.05.020
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: E.K.H. Schweda
  Institutions: Karolinska Institutet, Clinical Research Centre, Huddinge, Sweden
  Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, HF solvolysis, ESI-MS, mild acid hydrolysis, de-O-acylation with hydrazine, composition analysis, NMR-1D, alkaline deacylation, HPAEC-PAD, CE-ESI-MS/MS, CE-ESI-MS, LC-ESI-MS
  
   
  
  Haemophilus influenzae NTHi 1008
  CSDB ID 26994 (all data & tools)