Found 142 structures.
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1. Compound ID: 98
L-gro-a-D-manHepp-(1-7)-+
|
a-D-Galp-(1-6)-+ | a-Kdop-(2-4)-+
| | |
a-D-GlcpNAc-(1-2)-a-D-Glcp-(1-2)-a-D-Galp-(1-3)-a-D-Galp-(1-3)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-a-Kdop-(2--/lipid A/ |
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Structure type: oligomer
Aglycon: lipid A
Compound class: LPS
Contained glycoepitopes: IEDB_115013,IEDB_130645,IEDB_130650,IEDB_130659,IEDB_130670,IEDB_130693,IEDB_134624,IEDB_136906,IEDB_137472,IEDB_140088,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_190606,IEDB_2189047,IEDB_226811,IEDB_742246,IEDB_918313,IEDB_983931,SB_163,SB_192,SB_7,SB_87
The structure is contained in the following publication(s):
- Article ID: 18
Borrelli S, Hegedus O, Shaw DH, Jansson P, Lindberg AA "The tetrasaccharide L-a-D-heptose1->2-L-a-D-heptose1->3-L-a-D-heptose1->(3-deoxy-D-manno-octulosonic acid) and phosphate in lipid A define the conserved epitope in Haemophilus lipopolysaccharides recognized by a monoclonal antibody" -
Infection and Immunity 63 (1995) 3683-3692
A murine monoclonal antibody, MAHI 3 (immunoglobulin G2b), that is broadly reactive with Haemophilus influenzae lipopolysaccharides (LPSs) but nonreactive with all enterobacterial LPSs tested was generated by fusing mouse myeloma cells with spleen cells of BALB/c mice immunized with azide-killed H. influenzae RM.7004. MAHI 3 bound to all H. influenzae, all other human Haemophilus spp., all Bordetella pertussis and Bordetella parapertussis, and all Aeromonas spp. tested but not to any Neisseria or Moraxella catarrhalis strains, as determined by enzyme immunoassay, colony dot immunoblotting, and immunoblotting. In an inhibition enzyme immunoassay, MAHI 3 reacted with all 45 H. influenzae LPSs tested but not with the LPS from the rough mutant I69 Rd-/b+, which has only 3-deoxy-D-manno-octulosonic acid (P) [Kdop(P)] and lipid A. The antibody was not inhibited by H. influenzae lipid A or lipid-free polysaccharide isolated after mild acid hydrolysis. Only native LPSs show positive inhibitory activity, indicating that part of lipid A is involved in the binding of MAHI 3. From the results, it is indicated that the structural element recognized by MAHI 3 is Hep α1→2 Hep α1→3 Hep α1→ Kdo together with part of lipid A, including the phosphate
Lipopolysaccharide, Haemophilus, L-glycero-D-manno-heptose, lipopolysaccharides, LPS, core, tetrasaccharide, acid, Kdo, antibodies, antibody, conserved, epitope, lipid, lipid A, monoclonal, monoclonal antibodies, monoclonal antibody, phosphate, recognition
NCBI PubMed ID: 7543887Journal NLM ID: 0246127Publisher: American Society for Microbiology
Correspondence: Pererk.Jansson@kfc.m13.hs.sll.se
Institutions: Department of Immunology, Microbiology, Pathology and Infectious Diseases, Karolinska Institute, Huddinge, Sweden
Methods: de-O-acylation, SDS-PAGE, alkaline de-O-N-acylation, dephosphorylation, chemical analysis, EIA, inhibition studies, dot immunoblotting
- Article ID: 4317
Huang JX, Azad MA, Yuriev E, Baker MA, Nation RL, Li J, Cooper MA, Velkov T "Molecular Characterization of Lipopolysaccharide Binding to Human a-1-Acid Glycoprotein" -
Journal of Lipids 2012 (2012) 475153
The ability of AGP to bind circulating lipopolysaccharide (LPS) in plasma is believed to help reduce the proinflammatory effect of bacterial lipid A molecules. Here, for the first time we have characterized human AGP binding characteristics of the LPS from a number of pathogenic Gram-negative bacteria: Escherichia coli, Salmonella typhimurium, Klebsiella pneumonia, Pseudomonas aeruginosa, and Serratia marcescens. The binding affinity and structure activity relationships (SAR) of the AGP-LPS interactions were characterized by surface plasma resonance (SPR). In order to dissect the contribution of the lipid A, core oligosaccharide and O-antigen polysaccharide components of LPS, the AGP binding affinity of LPS from smooth strains, were compared to lipid A, Kdo2-lipid A, R(a), R(d), and R(e) rough LPS mutants. The SAR analysis enabled by the binding data suggested that, in addition to the important role played by the lipid A and core components of LPS, it is predominately the unique species- and strain-specific carbohydrate structure of the O-antigen polysaccharide that largely determines the binding affinity for AGP. Together, these data are consistent with the role of AGP in the binding and transport of LPS in plasma during acute-phase inflammatory responses to invading Gram-negative bacteria.
O-antigen, lipid A, gram negative bacteria, lipopolysaccharide-binding, AGP binding, SPR
NCBI PubMed ID: 23316371Publication DOI: 10.1155/2012/475153Journal NLM ID: 101553819Publisher: Cairo: Hindawi Pub Corp
Correspondence: Tony Velkov
Institutions: Institute for Molecular Bioscience, The University of Queensland, 306 Carmody Road, St. Lucia, QLD 4072, Australia, Drug Development and Innovation, Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, VIC 3052, Australia, Medicinal Chemistry, Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, VIC 3052, Australia, Priority Research Centre in Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
Methods: molecular modeling, fluorescence spectroscopy, surface plasmon resonance (SPR)
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2. Compound ID: 99
L-gro-a-D-manHepp-(1-7)-+
|
a-D-Galp-(1-6)-+ | a-Kdop-(2-4)-+
| | |
a-D-Glcp-(1-2)-a-D-Galp-(1-3)-a-D-Galp-(1-3)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-a-Kdop-(2--/lipid A/ |
Show graphically |
Structure type: oligomer
Aglycon: lipid A
Contained glycoepitopes: IEDB_115013,IEDB_130645,IEDB_130650,IEDB_130659,IEDB_130670,IEDB_134624,IEDB_136906,IEDB_137472,IEDB_140088,IEDB_141794,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_190606,IEDB_2189047,IEDB_226811,IEDB_742246,IEDB_918313,IEDB_983931,SB_163,SB_192,SB_7,SB_87
The structure is contained in the following publication(s):
- Article ID: 18
Borrelli S, Hegedus O, Shaw DH, Jansson P, Lindberg AA "The tetrasaccharide L-a-D-heptose1->2-L-a-D-heptose1->3-L-a-D-heptose1->(3-deoxy-D-manno-octulosonic acid) and phosphate in lipid A define the conserved epitope in Haemophilus lipopolysaccharides recognized by a monoclonal antibody" -
Infection and Immunity 63 (1995) 3683-3692
A murine monoclonal antibody, MAHI 3 (immunoglobulin G2b), that is broadly reactive with Haemophilus influenzae lipopolysaccharides (LPSs) but nonreactive with all enterobacterial LPSs tested was generated by fusing mouse myeloma cells with spleen cells of BALB/c mice immunized with azide-killed H. influenzae RM.7004. MAHI 3 bound to all H. influenzae, all other human Haemophilus spp., all Bordetella pertussis and Bordetella parapertussis, and all Aeromonas spp. tested but not to any Neisseria or Moraxella catarrhalis strains, as determined by enzyme immunoassay, colony dot immunoblotting, and immunoblotting. In an inhibition enzyme immunoassay, MAHI 3 reacted with all 45 H. influenzae LPSs tested but not with the LPS from the rough mutant I69 Rd-/b+, which has only 3-deoxy-D-manno-octulosonic acid (P) [Kdop(P)] and lipid A. The antibody was not inhibited by H. influenzae lipid A or lipid-free polysaccharide isolated after mild acid hydrolysis. Only native LPSs show positive inhibitory activity, indicating that part of lipid A is involved in the binding of MAHI 3. From the results, it is indicated that the structural element recognized by MAHI 3 is Hep α1→2 Hep α1→3 Hep α1→ Kdo together with part of lipid A, including the phosphate
Lipopolysaccharide, Haemophilus, L-glycero-D-manno-heptose, lipopolysaccharides, LPS, core, tetrasaccharide, acid, Kdo, antibodies, antibody, conserved, epitope, lipid, lipid A, monoclonal, monoclonal antibodies, monoclonal antibody, phosphate, recognition
NCBI PubMed ID: 7543887Journal NLM ID: 0246127Publisher: American Society for Microbiology
Correspondence: Pererk.Jansson@kfc.m13.hs.sll.se
Institutions: Department of Immunology, Microbiology, Pathology and Infectious Diseases, Karolinska Institute, Huddinge, Sweden
Methods: de-O-acylation, SDS-PAGE, alkaline de-O-N-acylation, dephosphorylation, chemical analysis, EIA, inhibition studies, dot immunoblotting
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3. Compound ID: 100
L-gro-a-D-manHepp-(1-7)-+
|
a-D-Galp-(1-6)-+ | a-Kdop-(2-4)-+
| | |
a-D-Galp-(1-3)-a-D-Galp-(1-3)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-a-Kdop-(2--/lipid A/ |
Show graphically |
Structure type: oligomer
Aglycon: lipid A
Contained glycoepitopes: IEDB_115013,IEDB_130645,IEDB_130650,IEDB_130659,IEDB_130670,IEDB_134624,IEDB_136906,IEDB_137472,IEDB_140088,IEDB_141794,IEDB_151528,IEDB_190606,IEDB_2189047,IEDB_226811,IEDB_742246,IEDB_918313,SB_163,SB_7,SB_87
The structure is contained in the following publication(s):
- Article ID: 18
Borrelli S, Hegedus O, Shaw DH, Jansson P, Lindberg AA "The tetrasaccharide L-a-D-heptose1->2-L-a-D-heptose1->3-L-a-D-heptose1->(3-deoxy-D-manno-octulosonic acid) and phosphate in lipid A define the conserved epitope in Haemophilus lipopolysaccharides recognized by a monoclonal antibody" -
Infection and Immunity 63 (1995) 3683-3692
A murine monoclonal antibody, MAHI 3 (immunoglobulin G2b), that is broadly reactive with Haemophilus influenzae lipopolysaccharides (LPSs) but nonreactive with all enterobacterial LPSs tested was generated by fusing mouse myeloma cells with spleen cells of BALB/c mice immunized with azide-killed H. influenzae RM.7004. MAHI 3 bound to all H. influenzae, all other human Haemophilus spp., all Bordetella pertussis and Bordetella parapertussis, and all Aeromonas spp. tested but not to any Neisseria or Moraxella catarrhalis strains, as determined by enzyme immunoassay, colony dot immunoblotting, and immunoblotting. In an inhibition enzyme immunoassay, MAHI 3 reacted with all 45 H. influenzae LPSs tested but not with the LPS from the rough mutant I69 Rd-/b+, which has only 3-deoxy-D-manno-octulosonic acid (P) [Kdop(P)] and lipid A. The antibody was not inhibited by H. influenzae lipid A or lipid-free polysaccharide isolated after mild acid hydrolysis. Only native LPSs show positive inhibitory activity, indicating that part of lipid A is involved in the binding of MAHI 3. From the results, it is indicated that the structural element recognized by MAHI 3 is Hep α1→2 Hep α1→3 Hep α1→ Kdo together with part of lipid A, including the phosphate
Lipopolysaccharide, Haemophilus, L-glycero-D-manno-heptose, lipopolysaccharides, LPS, core, tetrasaccharide, acid, Kdo, antibodies, antibody, conserved, epitope, lipid, lipid A, monoclonal, monoclonal antibodies, monoclonal antibody, phosphate, recognition
NCBI PubMed ID: 7543887Journal NLM ID: 0246127Publisher: American Society for Microbiology
Correspondence: Pererk.Jansson@kfc.m13.hs.sll.se
Institutions: Department of Immunology, Microbiology, Pathology and Infectious Diseases, Karolinska Institute, Huddinge, Sweden
Methods: de-O-acylation, SDS-PAGE, alkaline de-O-N-acylation, dephosphorylation, chemical analysis, EIA, inhibition studies, dot immunoblotting
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4. Compound ID: 128
b-D-Galp-(1-6)-b-D-Galp-(1-4)-+
|
-3)-a-D-Galp-(1-3)-a-L-Rhap-(1-2)-a-L-Rhap-(1-2)-a-D-Galp-(1-3)-a-D-Galp-(1- |
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Structure type: polymer chemical repeating unit
Compound class: EPS
Contained glycoepitopes: IEDB_115013,IEDB_130645,IEDB_130669,IEDB_133754,IEDB_136044,IEDB_136105,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_151528,IEDB_153201,IEDB_156493,IEDB_190606,IEDB_225177,IEDB_742246,IEDB_885823,IEDB_918313,SB_165,SB_166,SB_187,SB_195,SB_7,SB_87,SB_88
The structure is contained in the following publication(s):
- Article ID: 25
Bubb WA, Urashima T, Fujiwara R, Shinnai T, Ariga H "Structural characterization of the extracellular polysaccharide produced by Streptococcus thermophilus OR 901" -
Carbohydrate Research 301(1-2) (1997) 41-50
The exocellular polysaccharide of Streptococcus thermophilus OR 901, isolated from partially deproteinised whey, is a heteropolymer of D-galactopyranose and L-rhamnopyranose residues in the molar ratio 5:2. The structure was established by methylation analysis and 1D and 2D NMR spectroscopy of the native polysaccharide, in combination with characterisation of oligosaccharide fragments, obtained by partial acid hydrolysis, using methylation analysis and 1D 1H NMR spectroscopy. The polysaccharide has a branched heptasaccharide repeating unit with the following structure: [structure]
Lactic acid bacteria, Streptococcus thermophilus, Exopolysaccharide structure, NMR data
NCBI PubMed ID: 9228738Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: wab@biochem.usyd.edu.au
Institutions: Department of Biochemistry, University of Sydney, NSW, Australia
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, acid hydrolysis
- Article ID: 61
Faber EJ, Zoon P, Kamerling JP, Vliegenthart JFG "The exopolysaccharides produced by Streptococcus thermophilus Rs and Sts have the same repeating unit but differ in viscosity of their milk cultures" -
Carbohydrate Research 310(4) (1998) 269-276
The polysaccharides produced by Streptococcus thermophilus Rs and Sts in skimmed milk consist of D-Gal and L-Rha in a molar ratio of 5:2. Linkage analysis and 1D/2D NMR (1H and 13C) studies revealed that both polysaccharides have the same branched heptasaccharide repeating unit: [formula: see text] Remarkably, the two strains differ in their effects on the viscosity of stirred milk cultures. The milk culture of S. thermophilus Rs is non-ropy and affords 135 mg/L polysaccharide with an average molecular mass of 2.6 x 10(3) kDa. In contrast, the milk culture of S. thermophilus Sts is ropy and produces 127 mg/L polysaccharide with an average molecular mass of 3.7 x 10(3) kDa. Permeability measurements of non-stirred milk cultures of both strains suggest that both strains have a similar effect on the protein-polysaccharide network. Therefore, the only clear difference between both strains, which may cause the difference in ropiness of the milk cultures, is the difference in molecular mass of the polysaccharide
Streptococcus thermophilus, exopolysaccharide, ropiness, permeability, viscosity
NCBI PubMed ID: 9821263Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: kame@boc.chem.uu.nl
Institutions: Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, Utrecht, The Netherlands
Methods: NMR-2D, methylation, NMR, sugar analysis
- Article ID: 921
Leeflang BR, Faber EJ, Erbel P, Vliegenthart JFG "Structure elucidation of glycoprotein glycans and of polysaccharides by NMR spectroscopy" -
Journal of Biotechnology 77(1) (2000) 115-122
The applicability of 1H NMR spectroscopy for the determination of the primary and tertiary structure of carbohydrate-containing molecules is demonstrated. For classes of known compounds the characterization can be based on chemical shifts observed in 1D NMR spectra with or without the aid of a computer database. For more complex structure determinations 2D NMR techniques are required. Here the application of 2D NMR is demonstrated for the primary structure determination of two bacterial exopolysaccharides, for the spatial structure determination of a disaccharide and a glycoprotein hormone.
NMR, structure, polysaccharide, polysaccharides, NMR spectroscopy, glycan, structure elucidation, spectroscopy, elucidation, glycoprotein, glycoprotein glycans
NCBI PubMed ID: 10674218Journal NLM ID: 8411927Publisher: Amsterdam: Elsevier
Correspondence: b.r.leeflang@chem.uu.nl
Institutions: Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, The Netherlands
Methods: NMR-2D, NMR
- Article ID: 3405
Gorska S, Grycko P, Rybka J, Gamian A "Exopolysaccharides of lactic acid bacteria: structure and biosynthesis" -
Postȩpy Higieny i Medycyny Doświadczalnej [Polish] 61 (2007) 805-818
The group of lactic acid bacteria (LABs) includes four genera: Lactobacillus, Leuconostoc, Pediococcus, and Streptococcus. The most characteristic feature of this group of microorganisms is the production of lactic acid as a main product of carbohydrate metabolism. LABs are responsible for the fermentation of alimentary products and they also produce a variety of agents, among them exopolysaccharides (EPSs), which inhibit the growth of pathogenic bacteria. In this article on the different types of EPSs produced by LABs, data concerning their structure and biosynthesis are presented
biosynthesis, structure, Streptococcus, Lactic acid bacteria, exopolysaccharides, Lactococcus, Lactobacillus, Leuconostoc
NCBI PubMed ID: 18097339Journal NLM ID: 0421052Publisher: Warszawa: Panstwowy Zaklad Wydawnictw Lekarskich
Institutions: Laboratorium Mikrobiologii Lekarskiej, Instytut Immunologii i Terapii Doswiadczalnej PAN im. L. Hirszfelda we Wroclawiu
- Article ID: 5533
Zhou Y, Cui Y, Qu X "Exopolysaccharides of lactic acid bacteria: Structure, bioactivity and associations: A review" -
Carbohydrate Polymers 207 (2019) 317-332
The ability to exhibit various bioactivities is widespread in exopolysaccharide (EPS) of lactic acid bacteria (LAB), and it has been admittedly associated with large structural variability of these polymers. Exceptional bioactivities such as cholesterol-lowering, immunomodulating, antioxidant, antiviral and anticoagulant effects render these biopolymers vast commercial value for global market and application potentials in medicine sector. Therefore, an elaborate understanding of structure-to-function associations will be prerequisite to search natural and artificial EPSs for new applications in functional food, health and medicine fields. In this review, it is presented a significant overview of the latest advances in the field of EPS from genes to application. This review emphasized in the general biosynthesis pathway together with genetic modules, multiple structures, functions, and respective functional mechanisms of LAB-derived EPSs, and the relationships between their structure and bioactivity, which will help to exploit new bioactive drugs from LAB-derived EPS.
biosynthesis, structure, exopolysaccharide, mechanism, bioactivity, Structure-to-function association
NCBI PubMed ID: 30600013Publication DOI: 10.1016/j.carbpol.2018.11.093Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Y. Cui
Institutions: Department of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, China, Institute of Microbiology, Heilongjiang Academy of Sciences, Harbin, China
- Article ID: 5791
Knirel YA, Van Calsteren M "Bacterial exopolysaccharides" -
Book: Comprehensive Glycoscience: From Chemistry to Systems Biology. Reference Module in Chemistry, Molecular Sciences and Chemical Engineering (2021) 1-75
Bacterial extracellular polysaccharides are known as a cell-bound capsule, a sheath, or a slime, which is excreted into the environment. They play an important role in virulence of medical bacteria and plant-to-symbiont interaction and are used for serotyping of bacteria and production of vaccines. Some exopolysaccharides have commercial applications in industry, and claims of health benefits have been documented for an increasing number of them. Exopolysaccharides have diverse composition and structure, and some contain sugar and non-sugar components that are found in bacterial carbohydrates only. The present article provides an updated collection of the data on exopolysaccharides of various classes of gram-negative and gram-positive bacteria reported until the end of 2019. When known, biosynthesis pathways of exopolysaccharides are treated in a summary manner. References are made to structure and biosynthesis relatedness between exopolysaccharides of different bacterial taxa as well as between bacterial polysaccharides and mammalian glycosaminoglycans.
polysaccharide structure, Gram-negative bacteria, capsule, Biofilm, polysaccharide biosynthesis, gram-positive bacteria, Monosaccharide composition, Bacterial exopolysaccharide, non-sugar component
Publication DOI: 10.1016/B978-0-12-819475-1.00005-5Publisher: Elsevier
Correspondence: marie-rose.vancalsteren@canada.ca; yknirel@gmail.com
Editors: Barchi J, Kamerling H
Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Saint-Hyacinthe Research and Development Centre, Agriculture and Agri-Food Canada, Saint-Hyacinthe, QC, Canada
- Article ID: 5880
De Vuyst L, De Vin F "Exopolysaccharides from Lactic Acid Bacteria" -
Book: Comprehensive Glycoscience: From Chemistry to Systems Biology. Reference Module in Chemistry, Molecular Sciences and Chemical Engineering (2007) 477-519
carbohydrates, polysaccharides, Lactic acid bacteria, exopolysaccharides, glycolipids, glycoproteins, Glycomics
Publication DOI: 10.1016/B978-044451967-2/00129-XPublisher: Elsevier
Correspondence: ldvuyst@vub.ac.be
Editors: Barchi J, Kamerling H
Institutions: Department of Applied Biological Sciences and Engineering, Research Group of Industrial Microbiology and Food Biotechnology, Vrije Universiteit Brussel, Brussels, Belgium
- Article ID: 6166
Xu Z, Guo O, Zhang H, Xiong Z, Zhang X, Ai L "Structural characterisation of EPS of Streptococcus thermophilus S-3 and its application in milk fermentation" -
International Journal of Biological Macromolecules 178 (2021) 263-269
The application of Streptococcus thermophilus S-3 into yogurt production was studied and the structural properties of the generated exopolysaccharides (EPS-S3) were characterized. The proposed structure of EPS-S3 was obtained. EPS-S3 contained a high ratio of N-Acetyl-galactosamine with the Mw of 574 kDa, which was higher than that of AR333 (314 kD) leading to higher apparent viscosity. Streptococcus thermophilus strain S-3 was co-cultured with Lactobacillus delbrueckii for yogut production which highly increased the acidifying rate and post-acidification rate. The quality of the co-cultured yogurts in terms of apparent viscosity, syneresis capacity, water holding capacity and rheological properties were much better than that by using Lactobacillus bulgaricus only. The production mechanism of EPS-S3 from gene regulated level was also discussed which is helpful to facilitate the application of Streptococcus thermophilus strain into milk production.
NMR, Streptococcus thermophilus, exopolysaccharides, co-culture
NCBI PubMed ID: 33639187Publication DOI: 10.1016/j.ijbiomac.2021.02.173Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: ailianzhong@163.com
Institutions: Shanghai Engineering Research Center of Food Microbiology, School of Medical Instruments and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China, State Key Laboratory of Dairy Biotechnology, Technology Center Bright Dairy & Food Co., Ltd, Shanghai 200436, China, State Key Laboratory of Food Nutrition and Safety, College of Food Science and Technology, Tianjin University of Science and Technology, Tianjin, China
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, acid hydrolysis, HPLC, function analysis of gene clusters, yogurt production, yogurt quality evaluation
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5. Compound ID: 129
Structure type: oligomer
Contained glycoepitopes: IEDB_115013,IEDB_130645,IEDB_136044,IEDB_136095,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_149558,IEDB_151528,IEDB_190606,IEDB_742246,IEDB_918313,IEDB_918314,SB_165,SB_166,SB_187,SB_195,SB_7,SB_87,SB_88
The structure is contained in the following publication(s):
- Article ID: 25
Bubb WA, Urashima T, Fujiwara R, Shinnai T, Ariga H "Structural characterization of the extracellular polysaccharide produced by Streptococcus thermophilus OR 901" -
Carbohydrate Research 301(1-2) (1997) 41-50
The exocellular polysaccharide of Streptococcus thermophilus OR 901, isolated from partially deproteinised whey, is a heteropolymer of D-galactopyranose and L-rhamnopyranose residues in the molar ratio 5:2. The structure was established by methylation analysis and 1D and 2D NMR spectroscopy of the native polysaccharide, in combination with characterisation of oligosaccharide fragments, obtained by partial acid hydrolysis, using methylation analysis and 1D 1H NMR spectroscopy. The polysaccharide has a branched heptasaccharide repeating unit with the following structure: [structure]
Lactic acid bacteria, Streptococcus thermophilus, Exopolysaccharide structure, NMR data
NCBI PubMed ID: 9228738Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: wab@biochem.usyd.edu.au
Institutions: Department of Biochemistry, University of Sydney, NSW, Australia
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, acid hydrolysis
- Article ID: 1679
Avila JL, Rojas M, Rodas A, Convit J "Parasitic oligosaccharide residues recognized by patients with mucocutaneous and localized cutaneous leishmaniasis" -
American Journal of Tropical Medicine and Hygiene 47 (1992) 284-290
Humoral immune responses were studied in 118 Venezuelan patients with either active mucocutaneous (MCL) or localized cutaneous leishmaniasis (LCL). Most patients had elevated antibody levels to the six promastigote oligosaccharide residues studied: galactosyl(α 1-2)galactose, galactosyl(α 1-3)galactose, galactosyl(α 1-6)galactose, galactosyl(α 1-3)mannose, galactofuranosyl(β1-3)mannose, and galactocerebroside. Significantly higher antibody levels were found in patients with MCL against galactosyl(α 1-3)galactose and Leishmania tropica glycoinositol phospholipid (GIPL)-1, GIPL-2, and GIPL-3 compared with patients with LCL. For both clinical forms of American cutaneous leishmaniasis (ACL), the most reactive antigen was galactosyl(α 1-3)galactose, with elevated levels found in 63% and 79% of MCL and LCL patients, respectively. In patients with MCL and LCL, no significant relationship was found between antibody levels against a given oligosaccharide residue and clinical parameters such as age, leishmanin diameter, number of skin lesions, or time of evolution. It is noteworthy that 33% and 15% of MCL and LCL patients, respectively, did not have elevated antibody levels against the six different oligosaccharide residues studied. This suggests the presence of a subpopulation of non-humoral immunoreactive ACL patients. The relationship between abnormal levels of oligosaccharide antibodies and the final outcome of the disease remains to be established.
NCBI PubMed ID: 1524141Journal NLM ID: 0370507Publisher: Northbrook, IL: American Society of Tropical Medicine and Hygiene
Institutions: Instituto de Biomedicina, Caracas, Venezuela
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6. Compound ID: 132
Structure type: oligomer
Contained glycoepitopes: IEDB_115013,IEDB_130645,IEDB_136044,IEDB_136095,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_149558,IEDB_151528,IEDB_190606,IEDB_742246,IEDB_918313,IEDB_918314,SB_165,SB_166,SB_187,SB_195,SB_7,SB_87,SB_88
The structure is contained in the following publication(s):
- Article ID: 25
Bubb WA, Urashima T, Fujiwara R, Shinnai T, Ariga H "Structural characterization of the extracellular polysaccharide produced by Streptococcus thermophilus OR 901" -
Carbohydrate Research 301(1-2) (1997) 41-50
The exocellular polysaccharide of Streptococcus thermophilus OR 901, isolated from partially deproteinised whey, is a heteropolymer of D-galactopyranose and L-rhamnopyranose residues in the molar ratio 5:2. The structure was established by methylation analysis and 1D and 2D NMR spectroscopy of the native polysaccharide, in combination with characterisation of oligosaccharide fragments, obtained by partial acid hydrolysis, using methylation analysis and 1D 1H NMR spectroscopy. The polysaccharide has a branched heptasaccharide repeating unit with the following structure: [structure]
Lactic acid bacteria, Streptococcus thermophilus, Exopolysaccharide structure, NMR data
NCBI PubMed ID: 9228738Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: wab@biochem.usyd.edu.au
Institutions: Department of Biochemistry, University of Sydney, NSW, Australia
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, acid hydrolysis
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7. Compound ID: 710
P-4)-+ P-4)-+
| |
D-GalaNAc-(1-4:1-6)-a-D-Galp-(1-6)-a-D-Galp-(1-3)-a-D-Galp-(1--P--3)--D-gro-a-D-manHepp-(1-5)-a-Kdop8N-(2-6)-b-D-GlcpN-(1-6)-a-D-GlcpN-(1-P
xDGalaN = S-D-GalN (open chain) |
Show graphically |
Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_115013,IEDB_130645,IEDB_134624,IEDB_135394,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_141807,IEDB_145001,IEDB_151528,IEDB_151531,IEDB_190606,IEDB_2189046,IEDB_742246,IEDB_918313,SB_163,SB_7,SB_87
The structure is contained in the following publication(s):
- Article ID: 189
Vinogradov E, Korenevsky A, Beveridge TJ "The structure of the rough-type lipopolysaccharide from Shewanella oneidensis MR-1, containing 8-amino-8-deoxy-Kdo and an open-chain form of 2-acetamido-2-deoxy-D-galactose" -
Carbohydrate Research 338(19) (2003) 1991-1997
The LPS from Shewanella oneidensis strain MR-1 was analysed by chemical methods and by NMR spectroscopy and mass spectrometry. The LPS contained no polysaccharide O-chain, and its carbohydrate backbone had the following structure: (1S)-GalNAco-(1→4,6)-α-Gal-(1→6)-α-Gal-(1→3)-α-Gal-(1-P-3)-α-DDHep-(1→5)-α-8-aminoKdo4R-(2→6)-β-GlcN4P-(1→6)-α-GlcN1P, where R is P or xXEtNPP. There are several novel aspects to this LPS. It contains a novel linking unit between the core polysaccharide and lipid A moieties, namely 8-amino-3,8-dideoxy-D-manno-octulosonic acid (8-aminoKdo) and a residue of 2-acetamido-2-deoxy-D-galactose (N-acetylgalactosamine, GalNAco) in an open-chain form, linked as cyclic acetal to O-4 and O-6 of D-galactopyranose. The structure contains a phosphodiester linkage between the α-D-galactopyranose and D-glycero-D-manno-heptose (DDHep) residues.
LPS, core, Kdo, Shewanella
NCBI PubMed ID: 14499575Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: evguenii.vinogradov@nrc-cnrc.gc.ca
Institutions: Institute for Biological Sciences, National Research Council (NRC), 100 Sussex Dr., Ottawa ON K1A 0R6 Canada, Department of Microbiology, College of Biological Science, University of Guelph, Guelph ON N1G 2W1 Canada
Methods: NMR-2D, NMR, chemical methods
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8. Compound ID: 712
D-GalaNAc-(1-4:1-6)-a-D-Galp-(1-6)-a-D-Galp-(1-3)-a-D-Gal
xDGalaN = S-D-GalN (open chain) |
Show graphically |
Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_115013,IEDB_130645,IEDB_134624,IEDB_136906,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_151528,IEDB_190606,IEDB_742246,IEDB_918313,SB_163,SB_7,SB_87
The structure is contained in the following publication(s):
- Article ID: 189
Vinogradov E, Korenevsky A, Beveridge TJ "The structure of the rough-type lipopolysaccharide from Shewanella oneidensis MR-1, containing 8-amino-8-deoxy-Kdo and an open-chain form of 2-acetamido-2-deoxy-D-galactose" -
Carbohydrate Research 338(19) (2003) 1991-1997
The LPS from Shewanella oneidensis strain MR-1 was analysed by chemical methods and by NMR spectroscopy and mass spectrometry. The LPS contained no polysaccharide O-chain, and its carbohydrate backbone had the following structure: (1S)-GalNAco-(1→4,6)-α-Gal-(1→6)-α-Gal-(1→3)-α-Gal-(1-P-3)-α-DDHep-(1→5)-α-8-aminoKdo4R-(2→6)-β-GlcN4P-(1→6)-α-GlcN1P, where R is P or xXEtNPP. There are several novel aspects to this LPS. It contains a novel linking unit between the core polysaccharide and lipid A moieties, namely 8-amino-3,8-dideoxy-D-manno-octulosonic acid (8-aminoKdo) and a residue of 2-acetamido-2-deoxy-D-galactose (N-acetylgalactosamine, GalNAco) in an open-chain form, linked as cyclic acetal to O-4 and O-6 of D-galactopyranose. The structure contains a phosphodiester linkage between the α-D-galactopyranose and D-glycero-D-manno-heptose (DDHep) residues.
LPS, core, Kdo, Shewanella
NCBI PubMed ID: 14499575Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: evguenii.vinogradov@nrc-cnrc.gc.ca
Institutions: Institute for Biological Sciences, National Research Council (NRC), 100 Sussex Dr., Ottawa ON K1A 0R6 Canada, Department of Microbiology, College of Biological Science, University of Guelph, Guelph ON N1G 2W1 Canada
Methods: NMR-2D, NMR, chemical methods
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9. Compound ID: 714
Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_115013,IEDB_130645,IEDB_134624,IEDB_136044,IEDB_136095,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_149558,IEDB_151528,IEDB_190606,IEDB_742246,IEDB_918313,IEDB_918314,SB_163,SB_165,SB_166,SB_187,SB_195,SB_7,SB_87,SB_88
The structure is contained in the following publication(s):
- Article ID: 189
Vinogradov E, Korenevsky A, Beveridge TJ "The structure of the rough-type lipopolysaccharide from Shewanella oneidensis MR-1, containing 8-amino-8-deoxy-Kdo and an open-chain form of 2-acetamido-2-deoxy-D-galactose" -
Carbohydrate Research 338(19) (2003) 1991-1997
The LPS from Shewanella oneidensis strain MR-1 was analysed by chemical methods and by NMR spectroscopy and mass spectrometry. The LPS contained no polysaccharide O-chain, and its carbohydrate backbone had the following structure: (1S)-GalNAco-(1→4,6)-α-Gal-(1→6)-α-Gal-(1→3)-α-Gal-(1-P-3)-α-DDHep-(1→5)-α-8-aminoKdo4R-(2→6)-β-GlcN4P-(1→6)-α-GlcN1P, where R is P or xXEtNPP. There are several novel aspects to this LPS. It contains a novel linking unit between the core polysaccharide and lipid A moieties, namely 8-amino-3,8-dideoxy-D-manno-octulosonic acid (8-aminoKdo) and a residue of 2-acetamido-2-deoxy-D-galactose (N-acetylgalactosamine, GalNAco) in an open-chain form, linked as cyclic acetal to O-4 and O-6 of D-galactopyranose. The structure contains a phosphodiester linkage between the α-D-galactopyranose and D-glycero-D-manno-heptose (DDHep) residues.
LPS, core, Kdo, Shewanella
NCBI PubMed ID: 14499575Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: evguenii.vinogradov@nrc-cnrc.gc.ca
Institutions: Institute for Biological Sciences, National Research Council (NRC), 100 Sussex Dr., Ottawa ON K1A 0R6 Canada, Department of Microbiology, College of Biological Science, University of Guelph, Guelph ON N1G 2W1 Canada
Methods: NMR-2D, NMR, chemical methods
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10. Compound ID: 715
EtN-(1---P---P---4)-+ P-4)-+
| |
D-GalaNAc-(1-4:1-6)-a-D-Galp-(1-6)-a-D-Galp-(1-3)-a-D-Galp-(1--P--3)--D-gro-a-D-manHepp-(1-5)-a-Kdop8N-(2-6)-b-D-GlcpN-(1-6)-a-D-GlcpN-(1-P
xDGalaN = S-D-GalN (open chain) |
Show graphically |
Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_115013,IEDB_120354,IEDB_123890,IEDB_130645,IEDB_134624,IEDB_135394,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_141807,IEDB_145001,IEDB_151528,IEDB_151531,IEDB_190606,IEDB_2189046,IEDB_742246,IEDB_918313,SB_163,SB_7,SB_87
The structure is contained in the following publication(s):
- Article ID: 189
Vinogradov E, Korenevsky A, Beveridge TJ "The structure of the rough-type lipopolysaccharide from Shewanella oneidensis MR-1, containing 8-amino-8-deoxy-Kdo and an open-chain form of 2-acetamido-2-deoxy-D-galactose" -
Carbohydrate Research 338(19) (2003) 1991-1997
The LPS from Shewanella oneidensis strain MR-1 was analysed by chemical methods and by NMR spectroscopy and mass spectrometry. The LPS contained no polysaccharide O-chain, and its carbohydrate backbone had the following structure: (1S)-GalNAco-(1→4,6)-α-Gal-(1→6)-α-Gal-(1→3)-α-Gal-(1-P-3)-α-DDHep-(1→5)-α-8-aminoKdo4R-(2→6)-β-GlcN4P-(1→6)-α-GlcN1P, where R is P or xXEtNPP. There are several novel aspects to this LPS. It contains a novel linking unit between the core polysaccharide and lipid A moieties, namely 8-amino-3,8-dideoxy-D-manno-octulosonic acid (8-aminoKdo) and a residue of 2-acetamido-2-deoxy-D-galactose (N-acetylgalactosamine, GalNAco) in an open-chain form, linked as cyclic acetal to O-4 and O-6 of D-galactopyranose. The structure contains a phosphodiester linkage between the α-D-galactopyranose and D-glycero-D-manno-heptose (DDHep) residues.
LPS, core, Kdo, Shewanella
NCBI PubMed ID: 14499575Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: evguenii.vinogradov@nrc-cnrc.gc.ca
Institutions: Institute for Biological Sciences, National Research Council (NRC), 100 Sussex Dr., Ottawa ON K1A 0R6 Canada, Department of Microbiology, College of Biological Science, University of Guelph, Guelph ON N1G 2W1 Canada
Methods: NMR-2D, NMR, chemical methods
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11. Compound ID: 1025
L-Fucp-(1-3)-+
|
b-D-Galp-(1-6)-b-D-Galp-(1-4)-+ |
| |
-2)-a-D-Galp-(1-3)-a-D-Galp-(1-3)-a-D-Galp-(1-3)-a-L-Rhap-(1-2)-a-L-Rhap-(1- |
Show graphically |
Structure type: polymer chemical repeating unit
Compound class: EPS, CPS
Contained glycoepitopes: IEDB_115013,IEDB_130645,IEDB_130669,IEDB_133754,IEDB_136044,IEDB_136045,IEDB_136105,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_142489,IEDB_144562,IEDB_151528,IEDB_152214,IEDB_153201,IEDB_156493,IEDB_174333,IEDB_190606,IEDB_225177,IEDB_742246,IEDB_885823,IEDB_918313,SB_165,SB_166,SB_187,SB_195,SB_7,SB_86,SB_87,SB_88
The structure is contained in the following publication(s):
- Article ID: 311
Low D, Ahlgren JA, Horne D, McMahon DJ, Oberg CJ, Broadbent JR "Role of Streptococcus thermophilus MR-1C capsular exopolysaccharide in cheese moisture retention" -
Applied and Environmental Microbiology 64(6) (1998) 2147-2151
Recent work by our group has shown that an exopolysaccharide (EPS)-producing starter pair, Streptococcus thermophilus MR-1C and Lactobacillus delbrueckii subsp. bulgaricus MR-1R, can significantly increase moisture retention in low-fat mozzarella (D. B. Perry, D. J. McMahon, and C. J. Oberg, J. Dairy Sci. 80:799-805, 1997). The objectives of this study were to determine whether MR-1C, MR-1R, or both of these strains are required for enhanced moisture retention and to establish the role of EPS in this phenomenon. Analysis of low-fat mozzarella made with different combinations of MR-1C, MR-1R, and the non-EPS-producing starter culture strains S. thermophilus TA061 and Lactobacillus helveticus LH100 showed that S. thermophilus MR-1C was responsible for the increased cheese moisture level. To investigate the role of the S. thermophilus MR-1C EPS in cheese moisture retention, the epsE gene in this bacterium was inactivated by gene replacement. Low-fat mozzarella made with L. helveticus LH100 plus the non-EPS-producing mutant S. thermophilus DM10 had a significantly lower moisture content than did cheese made with strains LH100 and MR-1C, which confirmed that the MR-1C capsular EPS was responsible for the water-binding properties of this bacterium in cheese. Chemical analysis of the S. thermophilus MR-1C EPS indicated that the polymer has a novel basic repeating unit composed of D-galactose, L-rhamnose, and L-fucose in a ratio of 5:2:1.
capsular, Streptococcus, Streptococcus thermophilus, exopolysaccharide, retention
NCBI PubMed ID: 9603827Journal NLM ID: 7605801Publisher: American Society for Microbiology
Correspondence: Broadbnt@cc.usu.edu
Institutions: Department of Nutrition and Food Sciences, Utah State University, Logan, Utah 84322-8700, Biopolymer Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604, and Department of Microbiology, Weber State University, Ogden, Utah 84408-2506
- Article ID: 5880
De Vuyst L, De Vin F "Exopolysaccharides from Lactic Acid Bacteria" -
Book: Comprehensive Glycoscience: From Chemistry to Systems Biology. Reference Module in Chemistry, Molecular Sciences and Chemical Engineering (2007) 477-519
carbohydrates, polysaccharides, Lactic acid bacteria, exopolysaccharides, glycolipids, glycoproteins, Glycomics
Publication DOI: 10.1016/B978-044451967-2/00129-XPublisher: Elsevier
Correspondence: ldvuyst@vub.ac.be
Editors: Barchi J, Kamerling H
Institutions: Department of Applied Biological Sciences and Engineering, Research Group of Industrial Microbiology and Food Biotechnology, Vrije Universiteit Brussel, Brussels, Belgium
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12. Compound ID: 1164
Structure type: polymer chemical repeating unit
Compound class: EPS, O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115013,IEDB_130645,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_151528,IEDB_190606,IEDB_742246,IEDB_918313,SB_7,SB_87
The structure is contained in the following publication(s):
- Article ID: 353
Poveda A, Santamaria M, Bernabé M, Rivera A, Corzo J, Jiménez-Barbero J "Solution conformation and dynamics of an extracellular polysaccharide isolated from Bradyrhyzobium as deduced from 1H NMR off resonance ROESY and 13C NMR relaxation measurements" -
Carbohydrate Research 304(3-4) (1997) 219-228
The conformational and dynamical features of an extracellular branched deacetylated polysaccharide isolated from Bradyrhizobium (Chamaecytisus proliferus) have been investigated by homo and heteronuclear NMR methods. 1H NMR cross relaxation rates have been obtained for this polysaccharide through regular NOESY and ROESY spectra as well as by modern off resonance ROESY techniques. Local proton-proton correlation times as well as interproton distances have been obtained. 13C NMR relaxation parameters (T1, T2, NOE) have also been measured at two different magnetic fields and interpreted using different approximations based on the Lipari and Szabo model free approach. The analysis of the data indicates the existence of important flexibility for the different linkages of the polysaccharide. Motions in the range of several ns contribute to the relaxation of the macromolecule, although faster internal motions in the 600-800 ps time scales are also present. These time scales indicate that segmental motions as well as internal motions around the glycosidic linkages are the major sources of relaxation for this molecule at 299 K.
NMR, dynamics, relaxation, off resonance ROESY, model free approach
NCBI PubMed ID: 9468627Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: iqojj01@pinarl.csic.es
Institutions: Sercicio lnterdepartamental de lnvestigacidn, Universidad Autonoma de Madrid, Cantoblanco 28049 Madrid, Spain, Departamento de Bioqulmica y Biologfa Molecular, Universidad de la Laguna, 38071 Tenerife, Spain, Instituto Quimica Organica, CSIC, Juan de la Cierva 3, 28006 Madrid, Spain, Centro de lnvestigacion Basica, Smith Kline Beecham, Tres Cantos, Madrid, Spain
Methods: NMR-2D, NMR
- Article ID: 3540
Sahly H, Keisari Y, Crouch E, Sharon N, Ofek I "Recognition of bacterial surface polysaccharides by lectins of the innate immune system and its contribution to defense against infection: the case of pulmonary pathogens" -
Infection and Immunity 76(4) (2008) 1322-1332
no abstract available
recognition, surface polysaccharide, glycoepitope, innate immune system, lung lectins
NCBI PubMed ID: 18086817Journal NLM ID: 0246127Publisher: American Society for Microbiology
Correspondence: sahly@labor-lademannbogen.de
Institutions: Institute of Immunology, Clinical Pathology and Molecular Medicine, Lademannbogen 61-63, 22339 Hamburg, Germany
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13. Compound ID: 1226
Structure type: fragment of a bigger structure
Compound class: O-antigen
Contained glycoepitopes: IEDB_115013,IEDB_130645,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_149558,IEDB_151528,IEDB_156494,IEDB_190606,IEDB_2229966,IEDB_742246,IEDB_918313,IEDB_918314,SB_165,SB_166,SB_187,SB_195,SB_7,SB_87,SB_88
The structure is contained in the following publication(s):
- Article ID: 380
Skurnik M, Zhang L "Molecular genetics and biochemistry of Yersinia lipopolysaccharide" -
APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica 104(12) (1996) 849-872
Studies on the molecular genetics of bacterial LPS serve at least two main purposes: (i) to help develop an understanding of the biology, biochemistry and genetics of this bacterial surface macromolecule, and (ii) to provide a basis for both vaccine development and virulence experiments. Both of these goals have been the driving force in studies of Yersinia LPS carried out during the last decade. Here we will review the progress made in the molecular genetics and biochemistry of Yersinia LPS. A deep understanding has been achieved with respect to Y. enterocolitica serotype O:3, reaching as far as a detailed analysis of the gene clusters directing the biosynthesis of the outer core oligosaccharide and of the O-ag. The O-ag gene clusters of Y. enterocolitica serotype O:8 and Y. pseudotuberculosis serotypes O:2a and O:5a have also been cloned and partially characterized LPS biosynthesis of these Yersinia species includes examples of the two major variations recognized in the biosynthesis of this macromolecule: (i) homopolymeric or O-antigen polymerase-independent biosynthesis, and (ii) heteropolymeric or O-antigen polymerase-dependent biosynthesis.
Lipopolysaccharide, genetic, gene, genetics, O-antigen, biochemistry, Yersinia, molecular genetics
NCBI PubMed ID: 9048864Publication DOI: 10.1111/j.1699-0463.1996.tb04951.xJournal NLM ID: 8803400Publisher: Copenhagen: Munksgaard
Institutions: Turku Centre for Biotechnology, University of Turku, Finland, department of Medical Microbiology, University of Turku, Turku, Finland
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14. Compound ID: 1227
Structure type: fragment of a bigger structure
Compound class: O-antigen
Contained glycoepitopes: IEDB_115013,IEDB_130645,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_139421,IEDB_141794,IEDB_149558,IEDB_151528,IEDB_156494,IEDB_190606,IEDB_2229966,IEDB_742246,IEDB_918313,IEDB_918314,SB_165,SB_166,SB_187,SB_195,SB_7,SB_87,SB_88
The structure is contained in the following publication(s):
- Article ID: 380
Skurnik M, Zhang L "Molecular genetics and biochemistry of Yersinia lipopolysaccharide" -
APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica 104(12) (1996) 849-872
Studies on the molecular genetics of bacterial LPS serve at least two main purposes: (i) to help develop an understanding of the biology, biochemistry and genetics of this bacterial surface macromolecule, and (ii) to provide a basis for both vaccine development and virulence experiments. Both of these goals have been the driving force in studies of Yersinia LPS carried out during the last decade. Here we will review the progress made in the molecular genetics and biochemistry of Yersinia LPS. A deep understanding has been achieved with respect to Y. enterocolitica serotype O:3, reaching as far as a detailed analysis of the gene clusters directing the biosynthesis of the outer core oligosaccharide and of the O-ag. The O-ag gene clusters of Y. enterocolitica serotype O:8 and Y. pseudotuberculosis serotypes O:2a and O:5a have also been cloned and partially characterized LPS biosynthesis of these Yersinia species includes examples of the two major variations recognized in the biosynthesis of this macromolecule: (i) homopolymeric or O-antigen polymerase-independent biosynthesis, and (ii) heteropolymeric or O-antigen polymerase-dependent biosynthesis.
Lipopolysaccharide, genetic, gene, genetics, O-antigen, biochemistry, Yersinia, molecular genetics
NCBI PubMed ID: 9048864Publication DOI: 10.1111/j.1699-0463.1996.tb04951.xJournal NLM ID: 8803400Publisher: Copenhagen: Munksgaard
Institutions: Turku Centre for Biotechnology, University of Turku, Finland, department of Medical Microbiology, University of Turku, Turku, Finland
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15. Compound ID: 1840
Structure type: polymer chemical repeating unit
Compound class: EPS
Contained glycoepitopes: IEDB_115013,IEDB_130645,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_151528,IEDB_151770,IEDB_151771,IEDB_190606,IEDB_742246,IEDB_918313,SB_7,SB_87
The structure is contained in the following publication(s):
- Article ID: 579
D'Haeze W, Glushka J, De Rycke R, Holsters M, Carlson RW "Structural characterization of extracellular polysaccharides of Azorhizobium caulinodans and importance for nodule initiation on Sesbania rostrata" -
Molecular Microbiology 52(2) (2004) 485-500
Summary During lateral root base nodulation, the microsymbiont Azorhizobium caulinodans enters its host plant, Sesbania rostrata, via the formation of outer cortical infection pockets, a process that is characterized by a massive production of H(2)O(2). Infection threads guide bacteria from infection pockets towards nodule primordia. Previously, two mutants were constructed that produce lipopolysaccharides (LPSs) similar to one another but different from the wild-type LPS, and that are affected in extracellular polysaccharide (EPS) production. Mutant ORS571-X15 was blocked at the infection pocket stage and unable to produce EPS. The other mutant, ORS571-oac2, was impaired in the release from infection threads and was surrounded by a thin layer of EPS in comparison to the wild-type strain that produced massive amounts of EPS. Structural characterization revealed that EPS purified from cultured and nodule bacteria was a linear homopolysaccharide of α-1,3-linked 4,6-O-(1-carboxyethylidene)-d-galactosyl residues. In situ H(2)O(2) localization demonstrated that increased EPS production during early stages of invasion prevented the incorporation of H(2)O(2) inside the bacteria, suggesting a role for EPS in protecting the microsymbiont against H(2)O(2). In addition, ex planta assays confirmed a positive correlation between increased EPS production and enhanced protection against H(2)O(2).
extracellular polysaccharide, EPS, Azorhizobium, Azorhizobium caulinodans, EPS production
NCBI PubMed ID: 15066035Journal NLM ID: 8712028Publisher: Blackwell Publishing
Correspondence: wim@ccrc.uga.edu
Institutions: Complex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Road, Athens, GA 30602-4712, USA
Methods: 13C NMR, 1H NMR, GLC-MS, PAGE
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Next 15 structure(s)
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