CSDB glycosyltransferase search

4 glycosyltransferase activities have been found.
Please note that CSDB GT database covers only a few species: Arabidopsis thaliana, Escherichia coli, Saccharomyces cerevisiae, Acinetobacter baumannii.

Enzyme / Gene Activity Object References
Enzyme name: ugt51p
UniProt ID: Q06321*
CAZy family: GT1

Gene name: ATG26/UGT51
Gene GenBank ID: 850886*
Synthesized dimer: bDGlcp(1-3)Subst // Subst = β-sitosterol = SMILES O{3}[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])[C@@]2([H])CC[C@@]4(C)[C@]3([H])CC[C@]4([C@@H](C)CC[C@@H](CC)C(C)C)[H]


Donor (ID 33616): aDGlcp(1-P-P-5)xXnucU

Acceptor (ID 34896): Subst // Subst = β-sitosterol = SMILES O{3}[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])[C@@]2([H])CC[C@@]4(C)[C@]3([H])CC[C@]4([C@@H](C)CC[C@@H](CC)C(C)C)[H]

Status: semi-direct evidence in vivo
Confirmation methods: mutation; expression in E. coli; in vitro (homogenate); in vitro (membrane preparation)
ID: 299
Notes: UGT51 gene encodes UDP-glucose:sterol glucosyltransferase which can use different
sterols such as cholesterol, sitosterol, and ergosterol as sugar acceptors
Organism (ID 17220): Saccharomyces cerevisiae UTL-7A
Expressed in: E. coli

Full structure (ID 20490):

CSDB ID(s): 42839, 44557, 44558, 44559, 45044, 48154, 48199, 50368, 60932, 61476, 61969, 62123, 62299, 62387, 62464, 62469, 62504, 62746, 62753, 63027, 63214, 63289, 63445, 63676, 63755, 63892, 64042, 64128, 64648, 64665, 64819, 64883, 65044, 65049, 65223, 65264, 65268, 65276, 65365, 65400, 65452, 65589, 66158, 66192, 66656, 66748, 67062, 67301, 67327, 67759, 67799, 67955, 68341, 68586, 68733, 68900, 101110, 101132, 108826, 121975, 121976, 121977, 145377, 147448, 201132, 299859

Molecule role: membrane glycolipid

Warnecke et al. 1999
DOI: 10.1074/jbc.274.19.13048
Synthesized dimer: bDGlcp(1-3)Subst // Subst = β-sitosterol = SMILES O{3}[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])[C@@]2([H])CC[C@@]4(C)[C@]3([H])CC[C@]4([C@@H](C)CC[C@@H](CC)C(C)C)[H]


Donor (ID 33616): aDGlcp(1-P-P-5)xXnucU

Acceptor (ID 34896): Subst // Subst = β-sitosterol = SMILES O{3}[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])[C@@]2([H])CC[C@@]4(C)[C@]3([H])CC[C@]4([C@@H](C)CC[C@@H](CC)C(C)C)[H]

Status: semi-direct evidence in vivo
Confirmation methods: mutation; expression in E. coli; in vitro (homogenate); in vitro (membrane preparation)
ID: 512
Notes: UGT51 gene encodes UDP-glucose:sterol glucosyltransferase which can use different
sterols such as cholesterol, sitosterol, and ergosterol as sugar acceptors
Organism (ID 12218): Avena sativa
Expressed in: E. coli

Full structure (ID 20490):

CSDB ID(s): 42839, 45044, 48154, 48199, 50368, 61476, 61969, 62123, 62299, 62387, 62464, 62469, 62504, 62746, 62753, 63027, 63214, 63289, 63445, 63676, 63755, 63892, 64042, 64128, 64648, 64665, 64819, 64883, 65044, 65049, 65223, 65264, 65268, 65276, 65365, 65400, 65452, 65589, 66158, 66192, 66656, 66748, 67062, 67301, 67327, 67759, 67799, 67955, 68341, 68586, 68733, 68900, 290698, 299859

Molecule role: membrane glycolipid

Synthesized dimer: bDGlcp(1-3)Subst // Subst = β-sitosterol = SMILES O{3}[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])[C@@]2([H])CC[C@@]4(C)[C@]3([H])CC[C@]4([C@@H](C)CC[C@@H](CC)C(C)C)[H]


Donor (ID 33616): aDGlcp(1-P-P-5)xXnucU

Acceptor (ID 34896): Subst // Subst = β-sitosterol = SMILES O{3}[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])[C@@]2([H])CC[C@@]4(C)[C@]3([H])CC[C@]4([C@@H](C)CC[C@@H](CC)C(C)C)[H]

Status: semi-direct evidence in vivo
Confirmation methods: mutation; expression in E. coli; in vitro (homogenate); in vitro (membrane preparation)
ID: 517
Notes: UGT51 gene encodes UDP-glucose:sterol glucosyltransferase which can use different
sterols such as cholesterol, sitosterol, and ergosterol as sugar acceptors
Organism (ID 9097): Dictyostelium discoideum
Expressed in: E. coli

Full structure (ID 20490):

CSDB ID(s): 42839, 45044, 48154, 48199, 50368, 61476, 61969, 62123, 62299, 62387, 62464, 62469, 62504, 62746, 62753, 63027, 63214, 63289, 63445, 63676, 63755, 63892, 64042, 64128, 64648, 64665, 64819, 64883, 65044, 65049, 65223, 65264, 65268, 65276, 65365, 65400, 65452, 65589, 66158, 66192, 66656, 66748, 67062, 67301, 67327, 67759, 67799, 67955, 68341, 68586, 68733, 68900, 290698, 299859

Molecule role: membrane glycolipid

Synthesized dimer: bDGlcp(1-3)Subst // Subst = β-sitosterol = SMILES O{3}[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])[C@@]2([H])CC[C@@]4(C)[C@]3([H])CC[C@]4([C@@H](C)CC[C@@H](CC)C(C)C)[H]


Donor (ID 33616): aDGlcp(1-P-P-5)xXnucU

Acceptor (ID 34896): Subst // Subst = β-sitosterol = SMILES O{3}[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])[C@@]2([H])CC[C@@]4(C)[C@]3([H])CC[C@]4([C@@H](C)CC[C@@H](CC)C(C)C)[H]

Status: semi-direct evidence in vivo
Confirmation methods: mutation; expression in E. coli; in vitro (homogenate); in vitro (membrane preparation)
ID: 522
Notes: UGT51 gene encodes UDP-glucose:sterol glucosyltransferase which can use different
sterols such as cholesterol, sitosterol, and ergosterol as sugar acceptors
Organism (ID 10327): Pichia pastoris
Expressed in: E. coli

Full structure (ID 20490):

CSDB ID(s): 42839, 45044, 48154, 48199, 50368, 61476, 61969, 62123, 62299, 62387, 62464, 62469, 62504, 62746, 62753, 63027, 63214, 63289, 63445, 63676, 63755, 63892, 64042, 64128, 64648, 64665, 64819, 64883, 65044, 65049, 65223, 65264, 65268, 65276, 65365, 65400, 65452, 65589, 66158, 66192, 66656, 66748, 67062, 67301, 67327, 67759, 67799, 67955, 68341, 68586, 68733, 68900, 290698, 299859

Molecule role: membrane glycolipid

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